AQA A-Level Biology Paper 2, June 2025: Question 10
15 marks · Medium difficulty · Extended Answer
Explain animal biodiversity, the impact of abiotic factors on recycling rates, the role of bacteria in the nitrogen cycle, the process of secondary succession, and farming practices preventing succession in tropical rainforests.
Practise this questionQuestion
Question text
10 Read the following passage.
Tropical rainforests are among the most biologically diverse ecosystems in the
world. Their varied plant communities support a high biodiversity of animal
species. Tropical rainforests form in parts of the world with high annual rainfall
and high mean temperatures providing moist, warm conditions. Nutrient
recycling occurs rapidly on the ground of these rainforests. The recycling of 5
nitrogen-containing compounds from dead organic matter sustains the growth
of plants.
Each year, large areas of rainforests are lost due to fires. Natural regeneration
of rainforests occurs slowly by secondary succession. Secondary succession
is similar to primary succession but occurs in areas previously inhabited. 10
There is conflict between the needs of humans and conservation of rainforests.
Deforestation by humans is removing large areas of rainforests. Farming
practices have prevented the natural regeneration of rainforests by secondary
succession.
Use the information in the passage and your own knowledge to answer the
following questions.
10.1 In tropical rainforests, there is a high biodiversity of animal species (lines 1–3).
Explain why.
[3 marks]
10.2 Nutrient recycling occurs rapidly on the ground of tropical rainforests (lines 4–5).
Explain how two abiotic factors in these rainforests enable the rapid rate of recycling.
[2 marks]
10.3 The recycling of nitrogen-containing compounds from dead organic matter sustains
the growth of plants (lines 5–7).
Describe the roles of bacteria in this recycling.
[4 marks]
10.4 Natural regeneration of rainforests occurs by secondary succession (lines 8–10).
Describe the process of secondary succession.
[4 marks]
10.5 Farming practices have prevented the natural regeneration of rainforests by
secondary succession (lines 12–14).
Suggest two farming practices that prevent the natural regeneration of rainforests by
secondary succession.
[2 marks]
Mark scheme
Show the mark scheme
Question Marking Guidance Mark Comments
1. (Large) number/variety of plant 1. Accept high plant
species/populations; species richness or
high index of plant
2. (More/different) habitats/niches; diversity
10.1 3. (Greater) variety/types/sources of food; (3 x
AO2) 2. Ignore
shelter/homes
/environments
3. Ignore ‘more food’
1. (High/optimum) temperature for enzymes
2. Reject ‘water for
/respiration/nitrification/ammonification;
photosynthesis’
2. Water/moisture for metabolic/enzyme
4. Accept:
reactions
saprophytes or
2 max saprotrophs for
OR
10.2 (2 x saprobionts
AO2)
Water for hydrolysis;
3. (Optimum) pH for enzymes/respiration
/nitrification/ammonification;
4. (High) oxygen (concentration) for respiration–A-LEVEL BIOLOGY – 7402/2 –
/nitrification /nitrifying bacteria/ saprobionts;
1. Protein/amino acids/DNA into ammonium
1. Accept any named
/ammonia;
nitrogen containing
compound e.g. urea
2. (By) saprobionts;
2. Accept saprophytes
or saprotrophs
3. Ammonium/ammonia into nitrite and (then) into 1 and 3. Accept marks
nitrate; for conversion even if
incorrect type of
bacteria named as
4. (By) nitrifying bacteria/microorganisms; being involved
2 and 4. Reject marks
4 for type of bacteria if
10.3 (4 x linked to incorrect
AO1) process e.g. nitrite
converted to nitrate by
saprobionts
2 and 4. Accept
correctly named 21
species
Ignore nitrogen
fixation and
denitrification and
bacteria involved.
– A-LEVEL BIOLOGY – 7402/2 –
1. (Colonisation by) pioneer species; 1. 1. Accept (as
secondary
succession)
2. (Pioneers/species/organisms) change the ‘Seeds/spores
environment/habitat/conditions/factors; (present)’
2.Accept (as
3. (Environment becomes) less hostile for
secondary
other/new species
succession)
‘Soil/humus or
OR
organic matter is
already present
(Environment becomes) more suitable for
other/new species
2. Accept example of
change e.g. forms
OR
soil/humus/organic
4 max
matter/ nutrients
(Environment becomes) less suitable for (3 x
10.4
previous species; AO1, 1
22 2. Must convey idea
x AO2)
of change being
4. Change/increase in diversity/biodiversity;
caused by
pioneers/species/
5. (To) climax community (of rainforest);
organisms
3. Accept ‘favourable’
or ‘less harsh’ for
‘suitable’ but ignore
‘more hospitable’ or
‘more habitable’
3. Accept previous
species out-
competed.
– A-LEVEL BIOLOGY4. Ignore increase in–7402/2–
genetic diversity
1. Growing crops; 1 Accept specific
examples e.g.
2. Animal farming growing maize and
accept ‘monoculture’
OR
2. Accept examples
2 max e.g. cattle farming
(Land used for) grazing;
10.5 (2 x
3. Ploughing (land); AO2)
4. Accept insecticides
4. (Applying) weedkillers/herbicides/pesticides;
5. Reject burning fuel
5. Burning (fields/plants); 23
6. Building related to farming (barns/greenhouses);
How to answer it
Tropical Rainforest Ecosystems: Nutrient Cycling & Succession
What this question tests
This synoptic question links biodiversity, microbial nutrient cycling, and ecological succession within the context of tropical conservation and human intervention.
- Ecosystem interactions: Linking plant species diversity to animal niches and food supplies (AO2).
- Environmental factors on rates: How abiotic factors (temperature, moisture, O₂) alter metabolic rates in decomposers (AO2).
- Nitrogen cycle: Specific roles of saprobionts (ammonification) and nitrifying bacteria (nitrification) (AO1).
- Secondary succession: Sequential mechanism from pioneer colonisation to climax community (AO1/AO2).
- Anthropogenic impact: Explaining specific agricultural management practices that arrest succession (AO2).
Animal Biodiversity in Rainforests
Explain why in tropical rainforests, there is a high biodiversity of animal species.
✅ Mark Scheme Points
- (Large) number/variety of plant species (or plant populations).
- Creates more/different habitats or niches.
- Provides a (greater) variety/types/sources of food.
🧠 Exam Technique
Always build the ecological chain of causation for animal biodiversity:
High plant diversity → creates diverse microhabitats/niches + varied food types → supports many different adapted animal species.
❌ Common Errors & Examiner Traps
- Writing "more food": Examiner explicitly notes Ignore 'more food'. It is about variety of food, not sheer volume.
- Vague words for niche: Ignore 'shelter', 'homes', or 'environments'. Use the precise biological terms: niches or habitats .
💡 Key Knowledge
A niche is the role of an organism within its environment, including its interactions with biotic and abiotic factors. Varied plant architectures provide diverse vertical layers (canopy, understorey) creating numerous specialised niches.
Abiotic Factors & Rate of Recycling
Explain how two abiotic factors in these rainforests enable the rapid rate of recycling.
✅ Mark Scheme Points (Any Two)
- High/optimum temperature: Increases kinetic energy for faster enzyme-controlled reactions / respiration / ammonification / nitrification.
- Water/moisture: Required for metabolic/hydrolysis reactions or as a medium for enzyme action.
- Oxygen concentration: Required for aerobic respiration in decomposers/nitrifying bacteria.
- Near-neutral/optimum pH: Prevents enzyme denaturation in decomposers.
🧠 Exam Technique
This is a two-part explanation for each factor: Name the abiotic factor + Link it mechanistically to decomposer physiology (enzymes, respiration, or hydrolysis).
❌ Common Errors & Examiner Traps
- "Water for photosynthesis": REJECTED . The question asks about nutrient recycling on the ground (decomposition), not primary production!
- Leaving factor unqualified: Just stating "warmth" or "rain" without linking it to enzyme activity or chemical reactions scores 0.
💡 Key Knowledge
Saprobionts decompose dead organic matter via extracellular digestion: secreting enzymes (hydrolases) directly onto substrates. Both heat (kinetic energy) and water (medium & reactant for hydrolysis) directly control this rate.
Bacterial Roles in the Nitrogen Cycle
Describe the roles of bacteria in recycling nitrogen-containing compounds from dead organic matter.
✅ Mark Scheme Breakdown (4 Marks)
- Mark 1 (Conversion): Proteins / amino acids / DNA converted into ammonium / ammonia.
- Mark 2 (Bacteria): Carried out by saprobionts (saprotrophic bacteria/fungi).
- Mark 3 (Conversion): Ammonium / ammonia oxidised into nitrite, and then into nitrate.
- Mark 4 (Bacteria): Carried out by nitrifying bacteria.
💡 Key Knowledge: The Two Recycling Steps
- Ammonification:
Organic N (proteins/DNA/urea) → NH₄⁺ (by saprobionts) - Nitrification:
NH₄⁺ → NO₂⁻ (Nitrosomonas) → NO₃⁻ (Nitrobacter) (by aerobic nitrifying bacteria)
❌ Major Traps: Irrelevant Nitrogen Stages
- Writing about Nitrogen Fixation or Denitrification: The mark scheme states Ignore nitrogen fixation and denitrification. The stem specifies recycling from dead organic matter.
- Mismatched organisms: Saying "nitrite converted to nitrate by saprobionts" forfeits bacterial marks. Keep conversions strictly matched to organism names.
🧠 Exam Technique
Pair every biological chemical change directly with the specific class of microorganism performing it. State the specific ions: NH₄⁺ , NO₂⁻ , NO₃⁻ .
Process of Secondary Succession
Describe the process of secondary succession.
✅ Mark Scheme Points (Any 4 of 5)
- Colonisation by pioneer species (or germination of existing seeds/spores).
- Pioneers / organisms change the abiotic environment (e.g. add organic matter / humus / nutrients / form deeper soil).
- Environment becomes less hostile / more suitable for new/adapted species (OR less suitable for previous species / previous species outcompeted).
- Change / increase in biodiversity.
- Reaches a stable climax community.
🧠 Succession Model: "The Golden Formula"
Always structure succession questions using these 5 sequential steps:
- Pioneer arrives.
- Pioneer changes abiotic environment.
- Environment becomes less hostile for successors / previous outcompeted.
- Biodiversity increases.
- Climax community reached.
❌ Common Errors
- Confusing Primary with Secondary: In secondary succession, soil is already present. You do not need lichens breaking down bare rock!
- Vague words: Saying the environment becomes "more hospitable" or "habitable" is ignored. Use the syllabus term: less hostile or more suitable.
- "Genetic diversity": Stating "increase in genetic diversity" will not score for species biodiversity.
💡 Why Secondary Succession is Faster
Because soil, seeds, root fragments, and soil microbes already exist following disturbances like fires, secondary succession bypasses the lengthy soil-formation stage required in primary succession.
Agricultural Interruption of Succession
Suggest two farming practices that prevent the natural regeneration of rainforests by secondary succession.
✅ Mark Scheme Options (Any Two)
- Growing crops / Monoculture: Continuous harvesting and weeding prevents establishment of native seedlings.
- Animal farming / Livestock grazing: Animals eat young tree shoots/seedlings, trampling ground.
- Ploughing / Tilling: Mechanically destroys roots, seeds, and emergent pioneer plants.
- Application of herbicides / weedkillers: Kills broadleaf colonisers and tree saplings.
- Controlled burning / Clearing fields: Destroys emerging vegetation before trees can establish.
- Erecting farm buildings / structures: Covers soil completely with concrete/barns.
🧠 Exam Technique & Traps
❌ Reject: "Burning Fuel"
The mark scheme explicitly states: Reject burning fuel. You must state burning plants/fields (e.g. slash-and-burn farming).
Make sure your suggestions are genuine agricultural actions that continually disrupt pioneer plant survival (deflected succession / plagioclimax).
Topics
Biology · 3.4 Genetic information, variation and relationships between organisms · 3.5 Energy transfers in and between organisms (A-level only) · 3.7 Genetics, populations, evolution and ecosystems (A-level only)
Question and mark scheme from the AQA A-Level Biology examination, Paper 2, June 2025. QuestionVault is an independent revision resource; questions remain the copyright of the awarding body.