AQA A-Level Biology Paper 2, June 2025: Question 10

15 marks · Medium difficulty · Extended Answer

Explain animal biodiversity, the impact of abiotic factors on recycling rates, the role of bacteria in the nitrogen cycle, the process of secondary succession, and farming practices preventing succession in tropical rainforests.

Practise this question

Question

Question 10 starts with a short passage about tropical rainforests, their high biodiversity, warm and moist climate aiding rapid nutrient recycling, destruction by fires, regeneration by secondary succession, and the conflict with human farming practices. Sub-question 10.1 asks why there is high animal biodiversity in tropical rainforests (3 marks). 10.2 asks to explain how two abiotic factors enable rapid recycling (2 marks). 10.3 asks to describe the roles of bacteria in recycling nitrogen-containing compounds from dead organic matter (4 marks). 10.4 asks to describe the process of secondary succession (4 marks). 10.5 asks to suggest two farming practices preventing natural regeneration by secondary succession (2 marks).
Question text

10 Read the following passage.

Tropical rainforests are among the most biologically diverse ecosystems in the

world. Their varied plant communities support a high biodiversity of animal

species. Tropical rainforests form in parts of the world with high annual rainfall

and high mean temperatures providing moist, warm conditions. Nutrient

recycling occurs rapidly on the ground of these rainforests. The recycling of 5

nitrogen-containing compounds from dead organic matter sustains the growth

of plants.

Each year, large areas of rainforests are lost due to fires. Natural regeneration

of rainforests occurs slowly by secondary succession. Secondary succession

is similar to primary succession but occurs in areas previously inhabited. 10

There is conflict between the needs of humans and conservation of rainforests.

Deforestation by humans is removing large areas of rainforests. Farming

practices have prevented the natural regeneration of rainforests by secondary

succession.

Use the information in the passage and your own knowledge to answer the

following questions.

10.1 In tropical rainforests, there is a high biodiversity of animal species (lines 1–3).

Explain why.

[3 marks]

10.2 Nutrient recycling occurs rapidly on the ground of tropical rainforests (lines 4–5).

Explain how two abiotic factors in these rainforests enable the rapid rate of recycling.

[2 marks]

10.3 The recycling of nitrogen-containing compounds from dead organic matter sustains

the growth of plants (lines 5–7).

Describe the roles of bacteria in this recycling.

[4 marks]

10.4 Natural regeneration of rainforests occurs by secondary succession (lines 8–10).

Describe the process of secondary succession.

[4 marks]

10.5 Farming practices have prevented the natural regeneration of rainforests by

secondary succession (lines 12–14).

Suggest two farming practices that prevent the natural regeneration of rainforests by

secondary succession.

[2 marks]

Mark scheme

Show the mark scheme Mark scheme for Question 10. 10.1 awards 3 marks for large number/variety of plant species, more habitats/niches, and greater variety of food sources. 10.2 awards 2 marks for high temperature for enzymes/respiration/nitrification, and water/moisture for metabolic reactions or hydrolysis, or optimum pH, or high oxygen. 10.3 awards 4 marks for protein/amino acids/DNA into ammonium/ammonia by saprobionts, and ammonium into nitrite then nitrate by nitrifying bacteria. 10.4 awards 4 marks for pioneer species colonising, altering the environment, making it less hostile/more suitable for other species, leading to increased diversity up to a climax community. 10.5 awards 2 marks for two farming practices like grazing, ploughing, herbicide use, burning fields, or growing crops.

Question Marking Guidance Mark Comments

1. (Large) number/variety of plant 1. Accept high plant

species/populations; species richness or

high index of plant

2. (More/different) habitats/niches; diversity

10.1 3. (Greater) variety/types/sources of food; (3 x

AO2) 2. Ignore

shelter/homes

/environments

3. Ignore ‘more food’

1. (High/optimum) temperature for enzymes

2. Reject ‘water for

/respiration/nitrification/ammonification;

photosynthesis’

2. Water/moisture for metabolic/enzyme

4. Accept:

reactions

saprophytes or

2 max saprotrophs for

OR

10.2 (2 x saprobionts

AO2)

Water for hydrolysis;

3. (Optimum) pH for enzymes/respiration

/nitrification/ammonification;

4. (High) oxygen (concentration) for respiration–A-LEVEL BIOLOGY – 7402/2 –

/nitrification /nitrifying bacteria/ saprobionts;

1. Protein/amino acids/DNA into ammonium

1. Accept any named

/ammonia;

nitrogen containing

compound e.g. urea

2. (By) saprobionts;

2. Accept saprophytes

or saprotrophs

3. Ammonium/ammonia into nitrite and (then) into 1 and 3. Accept marks

nitrate; for conversion even if

incorrect type of

bacteria named as

4. (By) nitrifying bacteria/microorganisms; being involved

2 and 4. Reject marks

4 for type of bacteria if

10.3 (4 x linked to incorrect

AO1) process e.g. nitrite

converted to nitrate by

saprobionts

2 and 4. Accept

correctly named 21

species

Ignore nitrogen

fixation and

denitrification and

bacteria involved.

– A-LEVEL BIOLOGY – 7402/2 –

1. (Colonisation by) pioneer species; 1. 1. Accept (as

secondary

succession)

2. (Pioneers/species/organisms) change the ‘Seeds/spores

environment/habitat/conditions/factors; (present)’

2.Accept (as

3. (Environment becomes) less hostile for

secondary

other/new species

succession)

‘Soil/humus or

OR

organic matter is

already present

(Environment becomes) more suitable for

other/new species

2. Accept example of

change e.g. forms

OR

soil/humus/organic

4 max

matter/ nutrients

(Environment becomes) less suitable for (3 x

10.4

previous species; AO1, 1

22 2. Must convey idea

x AO2)

of change being

4. Change/increase in diversity/biodiversity;

caused by

pioneers/species/

5. (To) climax community (of rainforest);

organisms

3. Accept ‘favourable’

or ‘less harsh’ for

‘suitable’ but ignore

‘more hospitable’ or

‘more habitable’

3. Accept previous

species out-

competed.

– A-LEVEL BIOLOGY4. Ignore increase in–7402/2–

genetic diversity

1. Growing crops; 1 Accept specific

examples e.g.

2. Animal farming growing maize and

accept ‘monoculture’

OR

2. Accept examples

2 max e.g. cattle farming

(Land used for) grazing;

10.5 (2 x

3. Ploughing (land); AO2)

4. Accept insecticides

4. (Applying) weedkillers/herbicides/pesticides;

5. Reject burning fuel

5. Burning (fields/plants); 23

6. Building related to farming (barns/greenhouses);

How to answer it

Tropical Rainforest Ecosystems: Nutrient Cycling & Succession

What this question tests

This synoptic question links biodiversity, microbial nutrient cycling, and ecological succession within the context of tropical conservation and human intervention.

  • Ecosystem interactions: Linking plant species diversity to animal niches and food supplies (AO2).
  • Environmental factors on rates: How abiotic factors (temperature, moisture, O₂) alter metabolic rates in decomposers (AO2).
  • Nitrogen cycle: Specific roles of saprobionts (ammonification) and nitrifying bacteria (nitrification) (AO1).
  • Secondary succession: Sequential mechanism from pioneer colonisation to climax community (AO1/AO2).
  • Anthropogenic impact: Explaining specific agricultural management practices that arrest succession (AO2).
Question 10.1 • 3 Marks

Animal Biodiversity in Rainforests

Explain why in tropical rainforests, there is a high biodiversity of animal species.

✅ Mark Scheme Points

  1. (Large) number/variety of plant species (or plant populations).
  2. Creates more/different habitats or niches.
  3. Provides a (greater) variety/types/sources of food.

🧠 Exam Technique

Always build the ecological chain of causation for animal biodiversity:

High plant diversity → creates diverse microhabitats/niches + varied food types → supports many different adapted animal species.

❌ Common Errors & Examiner Traps

  • Writing "more food": Examiner explicitly notes Ignore 'more food'. It is about variety of food, not sheer volume.
  • Vague words for niche: Ignore 'shelter', 'homes', or 'environments'. Use the precise biological terms: niches or habitats .

💡 Key Knowledge

A niche is the role of an organism within its environment, including its interactions with biotic and abiotic factors. Varied plant architectures provide diverse vertical layers (canopy, understorey) creating numerous specialised niches.

Mark Allocation: 3 marks (AO2). 1 mark per bullet clearly stated.
Question 10.2 • 2 Marks

Abiotic Factors & Rate of Recycling

Explain how two abiotic factors in these rainforests enable the rapid rate of recycling.

✅ Mark Scheme Points (Any Two)

  • High/optimum temperature: Increases kinetic energy for faster enzyme-controlled reactions / respiration / ammonification / nitrification.
  • Water/moisture: Required for metabolic/hydrolysis reactions or as a medium for enzyme action.
  • Oxygen concentration: Required for aerobic respiration in decomposers/nitrifying bacteria.
  • Near-neutral/optimum pH: Prevents enzyme denaturation in decomposers.

🧠 Exam Technique

This is a two-part explanation for each factor: Name the abiotic factor + Link it mechanistically to decomposer physiology (enzymes, respiration, or hydrolysis).

❌ Common Errors & Examiner Traps

  • "Water for photosynthesis": REJECTED . The question asks about nutrient recycling on the ground (decomposition), not primary production!
  • Leaving factor unqualified: Just stating "warmth" or "rain" without linking it to enzyme activity or chemical reactions scores 0.

💡 Key Knowledge

Saprobionts decompose dead organic matter via extracellular digestion: secreting enzymes (hydrolases) directly onto substrates. Both heat (kinetic energy) and water (medium & reactant for hydrolysis) directly control this rate.

Mark Allocation: 2 marks max (AO2). 1 mark per explained factor.
Question 10.3 • 4 Marks

Bacterial Roles in the Nitrogen Cycle

Describe the roles of bacteria in recycling nitrogen-containing compounds from dead organic matter.

✅ Mark Scheme Breakdown (4 Marks)

  • Mark 1 (Conversion): Proteins / amino acids / DNA converted into ammonium / ammonia.
  • Mark 2 (Bacteria): Carried out by saprobionts (saprotrophic bacteria/fungi).
  • Mark 3 (Conversion): Ammonium / ammonia oxidised into nitrite, and then into nitrate.
  • Mark 4 (Bacteria): Carried out by nitrifying bacteria.

💡 Key Knowledge: The Two Recycling Steps

  1. Ammonification:
    Organic N (proteins/DNA/urea) → NH₄⁺ (by saprobionts)
  2. Nitrification:
    NH₄⁺ → NO₂⁻ (Nitrosomonas) → NO₃⁻ (Nitrobacter) (by aerobic nitrifying bacteria)

❌ Major Traps: Irrelevant Nitrogen Stages

  • Writing about Nitrogen Fixation or Denitrification: The mark scheme states Ignore nitrogen fixation and denitrification. The stem specifies recycling from dead organic matter.
  • Mismatched organisms: Saying "nitrite converted to nitrate by saprobionts" forfeits bacterial marks. Keep conversions strictly matched to organism names.

🧠 Exam Technique

Pair every biological chemical change directly with the specific class of microorganism performing it. State the specific ions: NH₄⁺ , NO₂⁻ , NO₃⁻ .

Mark Allocation: 4 marks (4 × AO1). Independent marks for conversions and naming correct bacteria.
Question 10.4 • 4 Marks

Process of Secondary Succession

Describe the process of secondary succession.

✅ Mark Scheme Points (Any 4 of 5)

  1. Colonisation by pioneer species (or germination of existing seeds/spores).
  2. Pioneers / organisms change the abiotic environment (e.g. add organic matter / humus / nutrients / form deeper soil).
  3. Environment becomes less hostile / more suitable for new/adapted species (OR less suitable for previous species / previous species outcompeted).
  4. Change / increase in biodiversity.
  5. Reaches a stable climax community.

🧠 Succession Model: "The Golden Formula"

Always structure succession questions using these 5 sequential steps:

  1. Pioneer arrives.
  2. Pioneer changes abiotic environment.
  3. Environment becomes less hostile for successors / previous outcompeted.
  4. Biodiversity increases.
  5. Climax community reached.

❌ Common Errors

  • Confusing Primary with Secondary: In secondary succession, soil is already present. You do not need lichens breaking down bare rock!
  • Vague words: Saying the environment becomes "more hospitable" or "habitable" is ignored. Use the syllabus term: less hostile or more suitable.
  • "Genetic diversity": Stating "increase in genetic diversity" will not score for species biodiversity.

💡 Why Secondary Succession is Faster

Because soil, seeds, root fragments, and soil microbes already exist following disturbances like fires, secondary succession bypasses the lengthy soil-formation stage required in primary succession.

Mark Allocation: 4 marks max (3 × AO1, 1 × AO2).
Question 10.5 • 2 Marks

Agricultural Interruption of Succession

Suggest two farming practices that prevent the natural regeneration of rainforests by secondary succession.

✅ Mark Scheme Options (Any Two)

  • Growing crops / Monoculture: Continuous harvesting and weeding prevents establishment of native seedlings.
  • Animal farming / Livestock grazing: Animals eat young tree shoots/seedlings, trampling ground.
  • Ploughing / Tilling: Mechanically destroys roots, seeds, and emergent pioneer plants.
  • Application of herbicides / weedkillers: Kills broadleaf colonisers and tree saplings.
  • Controlled burning / Clearing fields: Destroys emerging vegetation before trees can establish.
  • Erecting farm buildings / structures: Covers soil completely with concrete/barns.

🧠 Exam Technique & Traps

❌ Reject: "Burning Fuel"

The mark scheme explicitly states: Reject burning fuel. You must state burning plants/fields (e.g. slash-and-burn farming).

Make sure your suggestions are genuine agricultural actions that continually disrupt pioneer plant survival (deflected succession / plagioclimax).

Mark Allocation: 2 marks (2 × AO2). 1 mark per distinct farming practice.

Topics

Biology · 3.4 Genetic information, variation and relationships between organisms · 3.5 Energy transfers in and between organisms (A-level only) · 3.7 Genetics, populations, evolution and ecosystems (A-level only)

Question and mark scheme from the AQA A-Level Biology examination, Paper 2, June 2025. QuestionVault is an independent revision resource; questions remain the copyright of the awarding body.