AQA A-Level Biology Paper 3, June 2025: Question 6

15 marks · Medium difficulty · Extended Answer

Evaluate a dissection drawing of the human gas exchange system, describe the mechanism of inspiration, calculate lung volume from spirometer data, and evaluate clinical trial data for lung disease treatments.

Practise this question

Question

Question 6 consists of multiple parts based on a dissection of the animal gas exchange system and clinical lung conditions. Figure 5 depicts a detailed scientific drawing of the lungs, trachea, ribs, and diaphragm with labels A pointing to the ribs/intercostal muscles and B pointing to the diaphragm. Sub-question 6.1 asks for two ways to improve the scientific drawing. Sub-question 6.2 asks to describe the roles of structures A and B in increasing thoracic cavity volume. Sub-question 6.3 gives the helium dilution formula V2 = V1(C1 - C2)/C2 and requires calculating lung volume in dm³ given V1 = 3250 cm³, C1 = 10%, and C2 = 3.78%. Sub-questions 6.4 and 6.5 present context on asbestosis and ask for two additional diagnostic conditions and advantages of non-biopsy diagnosis. Sub-questions 6.6 and 6.7 present a survival curve (Figure 6) comparing nintedanib and pirfenidone in idiopathic pulmonary fibrosis patients over 2000 days, asking why there was no control group and evaluating whether these drugs should be considered for asbestosis.
Question text

06 A student completed a dissection of an animal gas exchange system.

Figure 5 shows the scientific drawing he produced from his observations.

Figure 5

06.1 Suggest two ways the scientific drawing in Figure 5 could be improved.

Do not refer to adding labels or using a sharp pencil in your answer.

[2 marks]

06.2 Describe the role of structures A and B in increasing the volume in the thoracic cavity.

[2 marks]

06.3 Lung volume can be determined with a spirometer using the helium dilution method.

The lung volume (V2) is calculated using the equation:

V1(C1 − C2)

V2 =

C2

where

C1 = the initial concentration of helium in the spirometer

C2 = the helium concentration in the spirometer when equal with the lungs

V1 = volume of gas in the spirometer.

A student used a spirometer with a volume of 3250 cm3 and an initial concentration of

10% helium. They found C2 was 3.78%

Use the equation to calculate this student’s lung volume in dm3

Show your working.

[2 marks]

20 3

Lung volume = dm

Asbestos is a fibrous mineral. If asbestos is inhaled, it can cause a lung disease

called asbestosis.

The latency period of a disease is the time between exposure to the cause of the

disease and the symptoms occurring. The latency period for asbestosis is

15 to 40 years.

Asbestosis can be diagnosed using a biopsy. This is where a sample of lung cells is

removed from a patient and examined with a microscope.

Asbestosis can also be diagnosed without a biopsy. To diagnose asbestosis without

a biopsy, five conditions must be met. Three of these are given below.

1. A scan of the lungs showing fibrous scar tissue.

2. Reduced gas exchange.

3. Shortness of breath.

These three conditions are also present in many other lung diseases.

06.4 Using the information provided, suggest the other two conditions that must be met

before a diagnosis of asbestosis can be made.

[2 marks]

06.5 Suggest two advantages of diagnosing asbestosis without a biopsy.

[2 marks]

Idiopathic pulmonary fibrosis (IPF) is a lung disease similar to asbestosis. Both

diseases cause:

• the death of alveolar epithelial cells due to DNA damage

• dead cells to be replaced by inelastic, fibrous scar tissue.

*20Currently, there is no cure for asbestosis and no effective treatment to slow its*

progression. There is also no cure for IPF. However, the drugs nintedanib and

pirfenidone can slow the formation of scar tissue and slow the progression of IPF.

In two separate clinical trials, doctors investigated the effect of nintedanib and

pirfenidone on 263 IPF patients.

• 124 patients were treated with nintedanib. 7 of these patients had to stop

treatment due to side effects.

• 139 patients were treated with pirfenidone. 14 of these patients had to stop

treatment due to side effects.

The doctors recorded how many days each patient lived after treatment.

Figure 6 shows their results.

Figure 6

06.6 Suggest why neither clinical trial had a control group.

[1 mark]

06.7 The doctors concluded that both drugs increased life expectancy in patients with IPF

compared with survival data for previous patients with IPF.

Using all the information, evaluate whether nintedanib and pirfenidone should be

considered as treatments for asbestosis.

[4 marks]

Section B

Answer one question.

You are advised to spend no more than 45 minutes on this section.

Mark scheme

Show the mark scheme The mark scheme provides criteria for Question 6 across all seven parts: 06.1 awards 2 marks for drawing improvements such as removing shading, drawing continuous lines, equal ribs, or adding a scale bar. 06.2 awards 2 marks for A contracting and pulling ribs up/out, and B contracting and flattening/moving down. 06.3 awards 2 marks for calculating 5.35 dm³ (accepting around 5.34788...). 06.4 awards 2 marks for history of exposure to asbestos and long latency period of 15–40 years. 06.5 awards 2 marks for advantages of non-biopsy (less invasive/painful, less risk of infection or further scar tissue, cheaper, quicker). 06.6 awards 1 mark for ethical reasons (unethical not to treat terminal patients). 06.7 awards up to 4 marks for evaluation points for and against considering the drugs for asbestosis.

Question Marking Guidance Mark Comments

1. Remove shading/hatching;

2. Have the same number of ribs on each side

OR

Make the sizes of the muscles/ribs more

accurate on each side;

3. Use single/continuous/joined line

OR

Do not use sketching

2 max

06.1 OR (2 x

AO3)

No crossing or hanging lines;

4. Not have the label line (for B) crossing/

obscuring the drawing;

5. (Add a) scale (bar); 5. Ignore (add)

magnification

6. Simplify the trachea/bronchus/bronchioles/

alveoli/structure 6. Accept reduce the

amount of detail

OR

Draw (only) the outline of tissues;

1. A contracts and pulls ribs up/out; If ‘contracts’ is not

mentioned, allow 1

mark for correct

2. B contracts and moves down; movement of both

structures

1. For A accept

(external)

intercostal muscle

2 For A reject

1.

06.2 (2 x internal intercostal

AO1) muscle

2. For B accept

diaphragm

2. For moves down

accept ‘flattens’

Answer of 5(.347883598) = 2 marks;; Accept any correct

rounding

Evidence of correct answer but incorrect rounding

e.g. 5.34 = 1 mark

OR

Evidence of 5347.883598 (answer in cm3)

= 1 mark

06.3 (2 x

OR AO2)

Evidence of numbers 5347883598 (ignore position

of decimal point, incorrect order of magnitude)

= 1mark

OR

Evidence of 20 215/20.215 (calculated the

numerator) = 1 mark;

1. (Known) contact with asbestos; 1. Accept

descriptions of

2. A latency period of 15–40 years contact with e.g.

exposure to

18 OR 1. Accept ‘(fibrous)

mineral’ for

A long latency period 2 'asbestos’

06.4 (2 x

OR AO3) 2. Accept a long time

between exposure

(Diagnosis) at least 15 years after; and symptoms

2. Accept any

number of given

years between 15

and 40 for latency

period/diagnosis

Accept converse for all

1. Less painful/invasive;

mark points in the

context of a biopsy

2. Less (risk of further) lung damage

1. Accept no surgery

OR (needed)

2 max

06.5 Less (risk of more) scar tissue; (2 x

AO3)

3. Less risk of infection/death;

4. Cheaper;

5. Quicker (procedure/results/recovery);

Unethical not to treat (control group); 1

06.6 (1 x

AO3)

Ignore answers

Max 3 for reasons FOR or AGAINST

relating to sample size

FOR (no mark)

1. Both (diseases) involve the formation of scar 9. Accept any

tissue reference to side

OR effects for or

against for 1 mark

Both (diseases) have similar symptoms 19

OR

(Drug/s) can slow the formation of scar tissue

OR

(Drug/s) can slow the symptoms/progression;

2. Both drugs equally as effective; 2. Must be clearly

stated and not

3. (Investigated) in humans/people; inferred

4. Long-term effects are known

OR

(It is) a long-term investigation;

AGAINST (no mark) 4 max

06.7 5. Is not a cure (4 x

AO3)

OR

Only reduces the formation of scar tissue;

6. Neither drug affects the cause/DNA damage;

7. Formation of scar tissue might

not be the same (for asbestosis)

OR

(Only) investigated in IPF

OR

Not investigated in asbestosis

OR

Needs investigating in asbestosis;

8. No control (group) to compare with 8. Accept

descriptions of

OR this, e.g. do not

know percentage

No group without treatment/drugs to compare of patients still

with alive without

treatment.

Question 7 Level of response marking guidance

Level of response marking instructions

Level of response mark schemes are broken down into levels, each of which has a descriptor. The

descriptor for the level shows the average performance for the level. There are marks in each level.

Before you apply the mark scheme to a student’s answer read through the answer and annotate it (as

instructed) to show the qualities that are being looked for. You can then apply the mark scheme.

Step 1 Determine a level

Start at the lowest level of the mark scheme and use it as a ladder to see whether the answer meets the

descriptor for that level. The descriptor for the level indicates the different qualities that might be seen in

the student’s answer for that level. If it meets the lowest level then go to the next one and decide if it

meets this level, and so on, until you have a match between the level descriptor and the answer. With

practice and familiarity you will find that for better answers you will be able to quickly skip through the

lower levels of the mark scheme.

When assigning a level you should look at the overall quality of the answer and not look to pick holes in

small and specific parts of the answer where the student has not performed quite as well as the rest. If

the answer covers different aspects of different levels of the mark scheme you should use a best fit

approach for defining the level and then use the variability of the response to help decide the mark within

the level, ie if the response is predominantly level 3 with a small amount of level 4 material it would be

placed in level 3 but be awarded a mark near the top of the level because of the level 4 content.

Step 2 Determine a mark

Once you have assigned a level you need to decide on the mark. The descriptors on how to allocate

marks can help with this. The exemplar materials used during standardisation will help. There will be an

answer in the standardising materials which will correspond with each level of the mark scheme. This

answer will have been awarded a mark by the Lead Examiner. You can compare the student’s answer

with the example to determine if it is the same standard, better or worse than the example. You can then

use this to allocate a mark for the answer based on the Lead Examiner’s mark on the example.

You may well need to read back through the answer as you apply the mark scheme to clarify points and

assure yourself that the level and the mark are appropriate.

Indicative content in the mark scheme is provided as a guide for examiners. It is not intended to be

exhaustive and you must credit other valid points. Students do not have to cover all of the points

mentioned in the Indicative content to reach the highest level of the mark scheme.

An answer which contains nothing of relevance to the question must be awarded no marks.

How to answer it

Gas Exchange System: Anatomy, Mechanics & Clinical Evaluation

📋 What this question tests

This question assesses required practical skills in biological drawing (rules and conventions), mechanics of pulmonary ventilation (role of intercostal muscles and diaphragm in inspiration), substitution and unit conversion calculations (spirometry and lung dilution), interpreting medical diagnostic criteria without biopsy, ethical considerations in clinical trials, and critical evaluation of clinical trial data regarding drug efficacy for fibrotic lung diseases.

Part 06.1

Improving a Scientific Drawing of the Gas Exchange System

Biological Drawing Conventions (2 Marks)

✅ Any TWO Mark Scheme Points

  • Remove shading / hatching.
  • Add a scale bar (Note: do not credit just "magnification").
  • Use single, continuous, unsketchy lines (no overlapping or hanging lines).
  • Ensure anatomical symmetry/accuracy (e.g. equal number of ribs on both sides, accurate muscle size).
  • Do not allow label lines to cross over or obscure parts of the drawing (e.g. line for B).
  • Simplify internal structure (only draw tissue outlines / reduce excess internal lines of bronchioles/alveoli).

❌ Common Errors & Disallowed Points

  • Ignored by instruction: The question explicitly says "Do not refer to adding labels or using a sharp pencil". Writing these scores 0.
  • Magnification vs Scale: Stating "add magnification" is not credited; biological drawings of dissections require an actual scale bar.
  • Vague comments: Saying "make it neater" or "draw it clearer" receives no credit. You must name the specific convention (e.g. "no shading").
Mark Allocation: 2 marks maximum [AO3 practical skills]. Each distinct valid biological drawing rule gives 1 mark.
Part 06.2

Mechanics of Inspiration: Structures A and B

Role of Intercostal Muscles and Diaphragm (2 Marks)

💡 Structure Identification

  • A: External intercostal muscles (attached to ribs).
  • B: Diaphragm (muscular sheet at the base of the thorax).

✅ Model Answer

  • Structure A (external intercostal muscles): contracts, pulling the ribs upwards and outwards. [1 mark]
  • Structure B (diaphragm): contracts and moves down / flattens. [1 mark]

🧠 Exam Technique: Always Mention the Action

Muscles only do one active thing: they contract. Many students describe the direction of movement (e.g. "ribs move up and out") but forget to state that A and B actively contract.

Examiner note: If "contracts" is missing from both, the mark scheme allows a maximum of 1 mark for stating the correct movement of both structures.

❌ Costly Mistakes

  • Stating that internal intercostal muscles contract during inspiration (they contract during forced expiration).
  • Stating that the diaphragm "relaxes" or "moves up into a dome shape" (that happens during expiration, which decreases volume).
Mark Allocation: 1 mark for A contracting & pulling ribs up/out; 1 mark for B contracting & flattening/moving down. (2 × AO1)
Part 06.3

Helium Dilution Calculation

Calculating Lung Volume (V2) with Unit Conversion (2 Marks)

📐 Step-by-Step Calculation

Equation: V2 = [V1 × (C1 − C2)] / C2

Given values:

  • Initial volume, V1 = 3250 cm³
  • Initial helium concentration, C1 = 10%
  • Equilibrium concentration, C2 = 3.78%

Step 1: Calculate the numerator
C1 − C2 = 10 − 3.78 = 6.22
V1 × (C1 − C2) = 3250 × 6.22 = 20 215

Step 2: Calculate V2 in cm³
V2 = 20 215 / 3.78 = 5347.883598... cm³

Step 3: Convert cm³ to dm³ (Required by answer line!)
Since 1 dm³ = 1000 cm³ , divide by 1000:
5347.883598 / 1000 = 5.35 dm³ (or 5.348 dm³ / 5.3 dm³)

Final Answer: 5.35 dm³

❌ Common Pitfalls

  • Forgetting unit conversion: Leaving the answer as 5348 cm³ caps your mark at 1 mark. The answer line specifically asks for dm³ .
  • Incorrect rounding: Writing 5.34 instead of correctly rounding 5.3478... up to 5.35 loses 1 mark if not showing full working.
Mark Allocation: 2 marks for final correct answer in dm³ (accept any correct rounding, e.g. 5.35, 5.348, 5.3). 1 mark partial credit for 5347.88... (answer in cm³), showing numerator 20 215, or incorrect rounding like 5.34.
Part 06.4

Diagnostic Criteria for Asbestosis

Differentiating from Other Lung Diseases (2 Marks)

💡 Context from the Stem

Three conditions are shared with other diseases: fibrous scar tissue scan, reduced gas exchange, and shortness of breath. The other two conditions must be uniquely specific to asbestosis as described in the text:

  1. Exposure to the specific causative agent (asbestos).
  2. The unique incubation/latency timeframe of the disease.

✅ Mark Scheme Answers (Any TWO)

  • History of exposure/contact with asbestos (or fibrous mineral). [1 mark]
  • A latency period of 15 to 40 years (accept: "long latency period", "diagnosis at least 15 years after exposure", or any specific value between 15 and 40 years). [1 mark]
Mark Allocation: 2 marks (2 × AO3). One mark for exposure to asbestos; one mark for latency period of 15–40 years.
Part 06.5

Advantages of Non-Invasive Diagnosis

Diagnosing Without a Biopsy (2 Marks)

✅ Any TWO Advantages

  • Less painful / non-invasive (no surgery required).
  • Less risk of further lung damage / less risk of forming additional scar tissue.
  • Lower risk of infection / complication / death.
  • Cheaper / lower cost for the healthcare provider.
  • Quicker (quicker procedure, faster turnaround of results, or quicker recovery time).

🧠 Exam Technique: Precise Biological Risks

When asked for advantages of avoiding a biopsy, always frame answers around patient trauma (pain, recovery time), surgical complications (infection risk, tissue puncture), and logistics (cost, speed).

Mark Allocation: 2 marks (2 × AO3). Accept converse points stated in the context of taking a biopsy.
Part 06.6

Ethics in Clinical Trials

Absence of a Control Group (1 Mark)

✅ Model Answer

It is unethical not to treat patients with a progressive, fatal condition (or unethical to give a placebo when patients have no other treatment options). [1 mark]

❌ Common Misconceptions

Students often incorrectly claim that "they ran out of patients", "controls weren't needed because they compared the two drugs to each other", or "IPF has no cure so a control is impossible". The mark scheme strictly rewards the ethical imperative: doctors cannot knowingly withhold potentially life-extending care from dying patients.

Mark Allocation: 1 mark (1 × AO3). Must mention ethical reason / unethical not to treat.
Part 06.7

Evaluating New Drug Treatments for Asbestosis

Data Evaluation: FOR vs AGAINST (4 Marks)

🧠 Structure of a 4-Mark Evaluation

You must present arguments FOR and AGAINST using all provided information (text, trial setup, and the Kaplan-Meier survival graph). The mark scheme caps either side at a maximum of 3 marks, meaning to score 4 marks you must include points from both sides.

Arguments FOR (Max 3 marks)

  • Similar pathology: Both diseases involve the formation of inelastic scar tissue / similar symptoms / both drugs slow scar tissue formation.
  • Similar effectiveness: Both drugs are equally effective at prolonging survival (survival curves in Figure 6 are almost identical). (Must be clearly stated).
  • Human trials: Both drugs were investigated in humans (not just animal models or cell cultures).
  • Long-term data: Long-term effects are known / trial followed patients for a long period (up to 2000 days).
  • Acceptable side effect rate: Only a minority had to stop treatment (7/124 for nintedanib and 14/139 for pirfenidone). [Can be credited FOR or AGAINST].

Arguments AGAINST (Max 3 marks)

  • Not a cure: Neither drug cures the disease; they only slow down the progression of scar tissue.
  • Cause remains untreated: Neither drug affects the underlying cause or DNA damage / cell death.
  • Tested on a different disease: Drugs were only investigated in IPF, not in asbestosis patients; scar tissue formation mechanisms might differ.
  • No untreated control group: No control group within the trial to directly compare survival against without treatment.
  • Side effects caused cessation: Some patients suffered side effects severe enough to stop treatment. [Can be credited FOR or AGAINST].

❌ Examiner Warning: Ignore Sample Size

The mark scheme explicitly instructs examiners to ignore answers relating to sample size (e.g. "small sample size of 263 patients" or "not enough patients"). Avoid relying on generic sample size comments in data evaluation questions.

Mark Allocation: 4 marks maximum [4 × AO3]. Max 3 marks for points strictly FOR or strictly AGAINST. At least one point must come from the opposing side for full marks.

Topics

Biology · Practical skills · Required Practicals · 3.3 Organisms exchange substances with their environment · Experimental design · Data analysis · AS practicals (1–6)

Question and mark scheme from the AQA A-Level Biology examination, Paper 3, June 2025. QuestionVault is an independent revision resource; questions remain the copyright of the awarding body.