AQA GCSE Biology Biology Paper 1 (Higher), November 2021: Question 4

14 marks · Standard Demand difficulty · Short Answer

Answer questions on aerobic and anaerobic respiration, identify the products and cellular locations, and explain changes in carbon dioxide concentration in a closed system containing pondweed and a snail.

Practise this question

Question

Exam question starting with parts on respiration equations, organelles, energy uses, and anaerobic respiration. A diagram in Figure 6 shows a boiling tube containing pondweed, a pond snail, and pond water sealed with a carbon dioxide probe leading to a data logger. Figure 7 is a line graph displaying carbon dioxide concentration from day 0 to 15. Carbon dioxide remains constant at 5.0 units for the first 5 days, increases after the tube is covered in black paper on day 5 to 6.5 units, levels off briefly around days 9–11, and increases to 7.4 units by day 15 after the snail dies on day 10.
Question text

04 All living organisms respire.

04.1 What is the chemical equation for aerobic respiration?

[1 mark]

Tick ( ) one box.

6O2 + 6CO2 6H2O + C6H12O6

6H2O + C6H12O6 6H2O + 6CO2

6H2O + 6CO2 6O2 + C6H12O6

6O2 + C6H12O6 6H2O + 6CO2

04.2 Name the sub-cellular structures where aerobic respiration takes place.

[1 mark]

04.3 Energy is released in respiration.

Give two uses of the energy released in respiration.

[2 marks]

04.4 Describe two differences between aerobic and anaerobic respiration in humans.

Do not refer to oxygen in your answer.

[2 marks]

04.5 What are the two products of anaerobic respiration in plant cells?

[2 marks]

Tick ( ) two boxes.

Carbon dioxide

Ethanol

Glucose

Lactic acid

Water

A scientist investigated respiration and photosynthesis using some pondweed and a

pond snail.

Figure 6 shows the apparatus used.

Figure 6

The apparatus was left in a well-lit room for 5 days.

The data logger recorded the concentration of carbon dioxide continuously.

After 5 days, the scientist completely covered the boiling tube with black paper.

The data logger continued to record the concentration of carbon dioxide.15

Figure 7 shows the concentration of carbon dioxide inside the boiling tube over

15 days.

Figure 7

04.6 Explain why the concentration of carbon dioxide in the tube stayed the same between

day 0 and day 5.

[2 marks]

04.7 Suggest why the concentration of carbon dioxide increased between day 5 and

day 10.

[1 mark]

04.8 On day 10, the pond snail died.

Explain why the death of the pond snail caused the concentration of carbon dioxide to

increase after day 10.

[3 marks]

Mark scheme

Show the mark scheme Mark scheme table for question 4. Lists correct answers: 04.1 correct equation '6O2 + C6H12O6 -> 6H2O + 6CO2'; 04.2 'mitochondria'; 04.3 any two from muscle contraction, keeping warm, active transport, building larger molecules; 04.4 any two differences between aerobic and anaerobic respiration in humans; 04.5 'carbon dioxide' and 'ethanol'; 04.6 pondweed taking in CO2 for photosynthesis and organisms respiring producing CO2; 04.7 no light so no photosynthesis while respiration continues; 04.8 decomposition of snail by microorganisms whose respiration releases CO2.

AO /

Question Answers Extra information Mark

Spec. Ref.

04.1 6O2 + C6H12O6 6H2O + 6CO2 1 AO1

4.4.2.1

04.2 mitochondria / mitochondrion 1 AO1

4.1.1.2

04.3 any two from: ignore reference to metabolism 2 AO1

unqualified 4.4.2.1

• movement / muscle allow examples of movement 4.4.2.3

contraction

• keeping warm

• active transport

• building larger molecules allow examples of building

larger molecules e.g. making

(named) proteins / cellulose

allow cell division

ignore growth

04.4 any two from: 2 AO1

• anaerobic produces lactic allow anaerobic creates an 4.4.2.1

acid and aerobic does not oxygen debt and aerobic does

not

• aerobic produces carbon

dioxide and anaerobic does

not

• aerobic produces water and

anaerobic does not

• aerobic occurs (mainly) in the allow anaerobic only occurs in

mitochondria and anaerobic the cytoplasm

does not

• anaerobic releases less allow anaerobic releases less

energy than aerobic ATP (than anaerobic)

do not accept anaerobic

produces / makes / creates less

energy

04.5 carbon dioxide 1 AO1

4.4.2.1

ethanol 1

04.6 pondweed takes in CO2 for 1 AO2

photosynthesis 4.4.2.1

4.4.1.1

snail and pondweed are 1

respiring producing CO2

if no other mark awarded allow

rate of respiration = rate of

photosynthesis for 1 mark

04.7 (no light so) no photosynthesis 1 AO2

or 4.4.2.1

plant is not taking in CO2 4.4.1.1

and

snail and plant are respiring and

so are releasing CO2

04.8 snail is being decayed / ignore being fed on 1 AO3

decomposed / broken down

(by) decomposers / bacteria (in allow fungi / microbes / 1 AO2

pond water / snail) microorganisms

(therefore) respiration (of do not accept anaerobic 1 AO3

decomposers / bacteria) respiration

releases CO2 4.7.2.2

4.4.2.1

Total 14

How to answer it

Respiration & Photosynthesis in an Aquatic Ecosystem

What this question tests

This multi-part question tests core biological recall alongside data interpretation and ecological understanding from Infection and response / Bioenergetics (Topic 4):

  • Recall: Balanced chemical equation for aerobic respiration, sub-cellular site (mitochondria), and biological uses of released energy.
  • Comparison: Key biochemical differences between aerobic and anaerobic respiration in humans and plant fermentation products.
  • Data Analysis & Application (AO2 & AO3): Explaining CO₂ dynamics over time involving photosynthetic uptake, cellular respiration, light deprivation, and microbial decomposition.
Part 04.1 • 1 Mark

Chemical Equation for Aerobic Respiration

Identify the correct balanced symbol equation

✅ Correct Answer

Tick Box 4:

6O₂ + C₆H₁₂O₆ → 6H₂O + 6CO₂

🧠 Exam Technique

Reactants are oxygen and glucose; products are water and carbon dioxide. Notice that Option 1 is unbalanced on hydrogens, Option 2 shows water as a reactant, and Option 3 is photosynthesis in reverse without glucose on the right.

Mark scheme: 1 mark for the fourth checkbox strictly.
Part 04.2 • 1 Mark

Site of Aerobic Respiration

Sub-cellular organelle

✅ Correct Answer

Mitochondria (or mitochondrion)

❌ Common Errors

Writing "nucleus", "ribosome", or "chloroplast". While plant cells have chloroplasts for photosynthesis, aerobic respiration occurs in the mitochondria in all eukaryotic cells.

Mark scheme: 1 mark for mitochondria / mitochondrion.
Part 04.3 • 2 Marks

Uses of Energy in Living Organisms

State two ways organisms use energy released from respiration

✅ Acceptable Answers (Any Two)

  • Movement / muscle contraction
  • Keeping warm / maintaining body temperature
  • Active transport (e.g. absorbing mineral ions against a concentration gradient)
  • Building larger molecules (e.g. protein synthesis, making cellulose or glycogen)
  • Cell division / mitosis

❌ Examiner Watch-outs

  • Do not write vague terms like "growth" or "metabolism" alone without detail.
  • Avoid simply saying "to survive" or "for energy" (circular argument).
Mark scheme: 1 mark for each valid use up to 2 marks.
Part 04.4 • 2 Marks

Aerobic vs Anaerobic Respiration in Humans

Describe two differences (Do NOT refer to oxygen)

✅ Correct Differences (Any Two)

  • Anaerobic produces lactic acid, whereas aerobic does not.
  • Aerobic produces carbon dioxide and water, whereas anaerobic does not.
  • Anaerobic releases much less energy than aerobic (or aerobic releases more energy / ATP).
  • Aerobic occurs in mitochondria; anaerobic occurs only in the cytoplasm.
  • Anaerobic creates an oxygen debt; aerobic does not.

❌ Major Examiner Trap

Never say respiration "produces" or "creates" energy! Energy cannot be created—it is released or transferred. Also, mentioning oxygen scores 0 because the question explicitly says: "Do not refer to oxygen in your answer."

Mark scheme: 2 marks total (1 mark for each valid direct comparison).
Part 04.5 • 2 Marks

Anaerobic Respiration in Plant Cells

Tick two products

✅ Correct Boxes to Tick

  • Carbon dioxide
  • Ethanol

💡 Key Knowledge

Anaerobic respiration in plant and yeast cells is known as fermentation:

Glucose → Ethanol + Carbon dioxide

In contrast, anaerobic respiration in animal muscles produces only lactic acid.

Mark scheme: 1 mark each for Carbon dioxide and Ethanol.
Part 04.6 • 2 Marks

Interpreting Day 0 to 5: Constant CO₂ Levels

Explain why the CO₂ concentration stayed constant

✅ Marking Points

  • Mark 1: Pondweed takes in CO₂ for photosynthesis.
  • Mark 2: Both the snail and pondweed are respiring and producing CO₂.

🧠 Exam Technique

For a steady state, the rate of removal must equal the rate of addition. Mention that both organisms respire; plants respire continuously day and night, not just animals!

Compensation rule: If neither specific point is awarded, stating that the rate of photosynthesis equals the rate of respiration scores 1 mark.

Mark scheme: 2 marks total.
Part 04.7 • 1 Mark

Interpreting Day 5 to 10: Darkness & CO₂ Rise

Suggest why CO₂ concentration increased after covering with black paper

✅ Full Answer (Needs Both Ideas)

With no light, there is no photosynthesis (the plant stops taking in CO₂), and both the snail and plant are still respiring and releasing CO₂.

❌ Common Error

Students often forget that the pondweed continues to respire in the dark. It is not just the snail releasing CO₂; both organisms release it, but none is absorbed because light is blocked.

Mark scheme: 1 mark for linking lack of photosynthesis to ongoing respiration releasing CO₂.
Part 04.8 • 3 Marks

Post-Day 10: Death of the Pond Snail

Explain why the snail's death caused CO₂ concentration to rise further

✅ 3-Step Marking Breakdown

  1. The snail's body is decayed / decomposed / broken down [1 mark]
  2. By decomposers / bacteria / fungi / microorganisms in the water [1 mark]
  3. These microorganisms respire, releasing CO₂ into the tube [1 mark]

🧠 Top Level Logic: The Decomposition Link

Students often lose marks by simply stating "the snail rotted and emitted gas". You must explain how decomposition works:

Dead biomass → Broken down by bacteria/fungi → Microbes respire aerobically → Releases CO₂.

Do NOT accept: Anaerobic respiration or "being fed on by other snails".

Mark scheme: 3 marks total (1 for decay/decomposition, 1 for naming decomposers/microbes, 1 for their respiration releasing CO₂).

Topics

Biology · B1: Cell Biology · B4: Bioenergetics · B7: Ecology

Question and mark scheme from the AQA GCSE Biology examination, Biology Paper 1 (Higher), November 2021. QuestionVault is an independent revision resource; questions remain the copyright of the awarding body.