AQA GCSE Biology Biology Paper 1 (Higher), November 2021: Question 4
14 marks · Standard Demand difficulty · Short Answer
Answer questions on aerobic and anaerobic respiration, identify the products and cellular locations, and explain changes in carbon dioxide concentration in a closed system containing pondweed and a snail.
Practise this questionQuestion
Question text
04 All living organisms respire.
04.1 What is the chemical equation for aerobic respiration?
[1 mark]
Tick ( ) one box.
6O2 + 6CO2 6H2O + C6H12O6
6H2O + C6H12O6 6H2O + 6CO2
6H2O + 6CO2 6O2 + C6H12O6
6O2 + C6H12O6 6H2O + 6CO2
04.2 Name the sub-cellular structures where aerobic respiration takes place.
[1 mark]
04.3 Energy is released in respiration.
Give two uses of the energy released in respiration.
[2 marks]
04.4 Describe two differences between aerobic and anaerobic respiration in humans.
Do not refer to oxygen in your answer.
[2 marks]
04.5 What are the two products of anaerobic respiration in plant cells?
[2 marks]
Tick ( ) two boxes.
Carbon dioxide
Ethanol
Glucose
Lactic acid
Water
A scientist investigated respiration and photosynthesis using some pondweed and a
pond snail.
Figure 6 shows the apparatus used.
Figure 6
The apparatus was left in a well-lit room for 5 days.
The data logger recorded the concentration of carbon dioxide continuously.
After 5 days, the scientist completely covered the boiling tube with black paper.
The data logger continued to record the concentration of carbon dioxide.15
Figure 7 shows the concentration of carbon dioxide inside the boiling tube over
15 days.
Figure 7
04.6 Explain why the concentration of carbon dioxide in the tube stayed the same between
day 0 and day 5.
[2 marks]
04.7 Suggest why the concentration of carbon dioxide increased between day 5 and
day 10.
[1 mark]
04.8 On day 10, the pond snail died.
Explain why the death of the pond snail caused the concentration of carbon dioxide to
increase after day 10.
[3 marks]
Mark scheme
Show the mark scheme
AO /
Question Answers Extra information Mark
Spec. Ref.
04.1 6O2 + C6H12O6 6H2O + 6CO2 1 AO1
4.4.2.1
04.2 mitochondria / mitochondrion 1 AO1
4.1.1.2
04.3 any two from: ignore reference to metabolism 2 AO1
unqualified 4.4.2.1
• movement / muscle allow examples of movement 4.4.2.3
contraction
• keeping warm
• active transport
• building larger molecules allow examples of building
larger molecules e.g. making
(named) proteins / cellulose
allow cell division
ignore growth
04.4 any two from: 2 AO1
• anaerobic produces lactic allow anaerobic creates an 4.4.2.1
acid and aerobic does not oxygen debt and aerobic does
not
• aerobic produces carbon
dioxide and anaerobic does
not
• aerobic produces water and
anaerobic does not
• aerobic occurs (mainly) in the allow anaerobic only occurs in
mitochondria and anaerobic the cytoplasm
does not
• anaerobic releases less allow anaerobic releases less
energy than aerobic ATP (than anaerobic)
do not accept anaerobic
produces / makes / creates less
energy
04.5 carbon dioxide 1 AO1
4.4.2.1
ethanol 1
04.6 pondweed takes in CO2 for 1 AO2
photosynthesis 4.4.2.1
4.4.1.1
snail and pondweed are 1
respiring producing CO2
if no other mark awarded allow
rate of respiration = rate of
photosynthesis for 1 mark
04.7 (no light so) no photosynthesis 1 AO2
or 4.4.2.1
plant is not taking in CO2 4.4.1.1
and
snail and plant are respiring and
so are releasing CO2
04.8 snail is being decayed / ignore being fed on 1 AO3
decomposed / broken down
(by) decomposers / bacteria (in allow fungi / microbes / 1 AO2
pond water / snail) microorganisms
(therefore) respiration (of do not accept anaerobic 1 AO3
decomposers / bacteria) respiration
releases CO2 4.7.2.2
4.4.2.1
Total 14
How to answer it
Respiration & Photosynthesis in an Aquatic Ecosystem
What this question tests
This multi-part question tests core biological recall alongside data interpretation and ecological understanding from Infection and response / Bioenergetics (Topic 4):
- Recall: Balanced chemical equation for aerobic respiration, sub-cellular site (mitochondria), and biological uses of released energy.
- Comparison: Key biochemical differences between aerobic and anaerobic respiration in humans and plant fermentation products.
- Data Analysis & Application (AO2 & AO3): Explaining CO₂ dynamics over time involving photosynthetic uptake, cellular respiration, light deprivation, and microbial decomposition.
Chemical Equation for Aerobic Respiration
Identify the correct balanced symbol equation
✅ Correct Answer
Tick Box 4:
6O₂ + C₆H₁₂O₆ → 6H₂O + 6CO₂
🧠 Exam Technique
Reactants are oxygen and glucose; products are water and carbon dioxide. Notice that Option 1 is unbalanced on hydrogens, Option 2 shows water as a reactant, and Option 3 is photosynthesis in reverse without glucose on the right.
Site of Aerobic Respiration
Sub-cellular organelle
✅ Correct Answer
Mitochondria (or mitochondrion)
❌ Common Errors
Writing "nucleus", "ribosome", or "chloroplast". While plant cells have chloroplasts for photosynthesis, aerobic respiration occurs in the mitochondria in all eukaryotic cells.
Uses of Energy in Living Organisms
State two ways organisms use energy released from respiration
✅ Acceptable Answers (Any Two)
- Movement / muscle contraction
- Keeping warm / maintaining body temperature
- Active transport (e.g. absorbing mineral ions against a concentration gradient)
- Building larger molecules (e.g. protein synthesis, making cellulose or glycogen)
- Cell division / mitosis
❌ Examiner Watch-outs
- Do not write vague terms like "growth" or "metabolism" alone without detail.
- Avoid simply saying "to survive" or "for energy" (circular argument).
Aerobic vs Anaerobic Respiration in Humans
Describe two differences (Do NOT refer to oxygen)
✅ Correct Differences (Any Two)
- Anaerobic produces lactic acid, whereas aerobic does not.
- Aerobic produces carbon dioxide and water, whereas anaerobic does not.
- Anaerobic releases much less energy than aerobic (or aerobic releases more energy / ATP).
- Aerobic occurs in mitochondria; anaerobic occurs only in the cytoplasm.
- Anaerobic creates an oxygen debt; aerobic does not.
❌ Major Examiner Trap
Never say respiration "produces" or "creates" energy! Energy cannot be created—it is released or transferred. Also, mentioning oxygen scores 0 because the question explicitly says: "Do not refer to oxygen in your answer."
Anaerobic Respiration in Plant Cells
Tick two products
✅ Correct Boxes to Tick
- Carbon dioxide
- Ethanol
💡 Key Knowledge
Anaerobic respiration in plant and yeast cells is known as fermentation:
Glucose → Ethanol + Carbon dioxide
In contrast, anaerobic respiration in animal muscles produces only lactic acid.
Interpreting Day 0 to 5: Constant CO₂ Levels
Explain why the CO₂ concentration stayed constant
✅ Marking Points
- Mark 1: Pondweed takes in CO₂ for photosynthesis.
- Mark 2: Both the snail and pondweed are respiring and producing CO₂.
🧠 Exam Technique
For a steady state, the rate of removal must equal the rate of addition. Mention that both organisms respire; plants respire continuously day and night, not just animals!
Compensation rule: If neither specific point is awarded, stating that the rate of photosynthesis equals the rate of respiration scores 1 mark.
Interpreting Day 5 to 10: Darkness & CO₂ Rise
Suggest why CO₂ concentration increased after covering with black paper
✅ Full Answer (Needs Both Ideas)
With no light, there is no photosynthesis (the plant stops taking in CO₂), and both the snail and plant are still respiring and releasing CO₂.
❌ Common Error
Students often forget that the pondweed continues to respire in the dark. It is not just the snail releasing CO₂; both organisms release it, but none is absorbed because light is blocked.
Post-Day 10: Death of the Pond Snail
Explain why the snail's death caused CO₂ concentration to rise further
✅ 3-Step Marking Breakdown
- The snail's body is decayed / decomposed / broken down [1 mark]
- By decomposers / bacteria / fungi / microorganisms in the water [1 mark]
- These microorganisms respire, releasing CO₂ into the tube [1 mark]
🧠 Top Level Logic: The Decomposition Link
Students often lose marks by simply stating "the snail rotted and emitted gas". You must explain how decomposition works:
Dead biomass → Broken down by bacteria/fungi → Microbes respire aerobically → Releases CO₂.
Do NOT accept: Anaerobic respiration or "being fed on by other snails".
Topics
Biology · B1: Cell Biology · B4: Bioenergetics · B7: Ecology
Question and mark scheme from the AQA GCSE Biology examination, Biology Paper 1 (Higher), November 2021. QuestionVault is an independent revision resource; questions remain the copyright of the awarding body.