AQA GCSE Biology Biology Paper 1 (Foundation), June 2022: Question 3
8 marks · Standard Demand difficulty · Short Answer
Analyse data on the effect of carbon dioxide concentration on the rate of photosynthesis in tomato plants, identify control variables, and explain limiting factors.
Practise this questionQuestion
Question text
03 A scientist investigated the rate of photosynthesis of one type of tomato plant.
The tomato plants were grown in a greenhouse.
Table 2 shows the results.
Table 2
Percentage (%)
Rate of photosynthesis
concentration of
in arbitrary units
carbon dioxide in the air
0.00 0
0.02 5
0.04 16
0.06 19
0.08 20
0.10 20
0.12 20
03.1 Give two control variables the scientist should have used in the investigation.
[2 marks]
03.2 Which range of carbon dioxide concentrations caused the rate of photosynthesis to
change the most?
[1 mark]
Tick ( ) one box.
From 0.00% to 0.02%
From 0.02% to 0.04%
From 0.04% to 0.06%
From 0.06% to 0.08% 11
03.3 How could the scientist have improved the validity of the results?
[1 mark]
Tick ( ) one box.
Repeat each reading three times and calculate a mean.
Use concentrations of carbon dioxide above 0.12%.
Use different tomato plants for each concentration.
03.4 Explain the change in the rate of photosynthesis when the concentration of
carbon dioxide increased between 0.00% to 0.08%.
[2 marks]
03.5 A farmer decided not to use a concentration of carbon dioxide higher than 0.08%
to grow tomato plants.
Suggest two reasons for the farmer’s decision.
Use information from Table 2 and your own knowledge.
[2 marks]
Mark scheme
Show the mark scheme
Question 3
AO /
Question Answers Extra information Mark
Spec. Ref.
03.1 any two from: 2 AO3
4.4.1.2
• temperature RPA6
• size of tomato plants or size / allow age of plant
number of leaves
• light
• (volume of) water allow (amount of) water
• (amount / type of) fertiliser / allow (type of) compost / soil
minerals / ions / nutrients allow named example of mineral
(given to plants) ion such as nitrate / magnesium
• time before rate readings are ignore time unqualified
taken
ignore type of tomato plant
ignore type of greenhouse
AO /
Spec. Ref.
03.2 from 0.02% to 0.04% 1 AO3
4.4.1.2
AO /
Spec. Ref.
03.3 repeat each reading three times 1 AO3
and calculate a mean 4.4.1.2
RPA6
AO /
Spec. Ref.
03.4 (the rate of photosynthesis) ignore values 1 AO2
increases 4.4.1.1
4.4.1.2
(because) carbon dioxide is 1
needed for photosynthesis
allow 2 marks for (there is) more
carbon dioxide for (more)
photosynthesis
AO /
Spec. Ref.
03.5 any two from: 2 AO3
4.4.1.2
• it would not increase the rate allow it would not change the
(of photosynthesis) rate (of photosynthesis)
allow photosynthesis would not
increase
• it would not increase the
growth of tomatoes
• it would cost more allow idea of profit will not
increase
allow reference to avoiding
global warming
Total Question 3 8
How to answer it
Photosynthesis & Limiting Factors: Tomato Plant Investigation
This question assesses your understanding of AQA Biology Topic 4.4 (Bioenergetics). You need to identify control variables to ensure a fair test, interpret rate data from a table, select methods to increase experimental validity, explain the biological effect of a limiting reactant (CO₂), and evaluate the economic costs and benefits for commercial crop growers.
Control Variables in Plant Experiments
Give two control variables the scientist should have used in the investigation.
✅ Correct Answers (Choose any two)
- Temperature
- Light intensity (or light wavelength / duration)
- Volume of water (or amount of water)
- Size / age of tomato plants (or number/surface area of leaves)
- Amount / type of fertiliser / mineral ions (e.g. nitrate, magnesium) or compost type
- Time before rate readings are recorded
🧠 Exam Technique
Read the question stem carefully: it already states "one type of tomato plant" and that they are in a "greenhouse". Never give a variable that has already been explicitly stated as fixed in the text!
❌ Common Errors
- Writing just "time" unqualified (you must state time before readings are taken or duration of exposure).
- Writing "type of tomato plant" (already controlled in the question stem).
- Writing "greenhouse" (not a measurable variable).
- Stating "carbon dioxide" (this is the independent variable, not a control variable!).
Analysing Changes in Rate from Table Data
Which range of carbon dioxide concentrations caused the rate of photosynthesis to change the most?
✅ Correct Answer
[✓] From 0.02% to 0.04%
📐 Step-by-Step Data Check
Calculate the difference (increase in rate) across each interval:
- 0.00% → 0.02%: 5 − 0 = +5
- 0.02% → 0.04%: 16 − 5 = +11 (Largest increase!)
- 0.04% → 0.06%: 19 − 16 = +3
- 0.06% → 0.08%: 20 − 19 = +1
🧠 Exam Technique
Never rely on a quick glance. Always do quick mental or written subtractions in the margin for each interval given in the options to avoid picking the initial or steepest apparent step by mistake.
Improving Experimental Validity & Repeatability
How could the scientist have improved the validity of the results?
✅ Correct Answer
[✓] Repeat each reading three times and calculate a mean.
💡 Key Knowledge: Why Repeats Matter
Repeating measurements and calculating a mean:
- Allows you to identify and discard anomalies.
- Reduces the effect of random measurement errors.
- Increases the reliability and validity of your conclusion.
❌ Why the other choices are wrong
- "Use concentrations of carbon dioxide above 0.12%": This only extends the range; it does not check whether current values are accurate.
- "Use different tomato plants for each concentration": This introduces plant-to-plant genetic variation as an uncontrolled variable, which actually reduces validity.
Explaining the Trend: Carbon Dioxide as a Limiting Factor
Explain the change in the rate of photosynthesis when the concentration of carbon dioxide increased between 0.00% to 0.08%.
✅ Correct Marking Points
- Mark 1 (Observation/Trend): The rate of photosynthesis increases.
- Mark 2 (Biological Reason): (Because) carbon dioxide is a reactant / needed for photosynthesis (or CO₂ was the limiting factor).
💡 Chemical Context
Recall the word and chemical equations for photosynthesis:
carbon dioxide + water → glucose + oxygen
6CO₂ + 6H₂O → C₆H₁₂O₆ + 6O₂
Between 0.00% and 0.08%, carbon dioxide is the limiting factor. Providing more reactant molecules allows photosynthesis to proceed faster.
🧠 "Explain" Command Word Strategy
An explain question always needs two parts:
- Say what happens: The rate increases.
- Say why it happens: Because CO₂ is required as a reactant.
Examiner note: Do not just quote numbers from the table—the mark scheme specifically states "ignore values" here.
Commercial Decisions: Economics vs Plant Yield
Suggest two reasons for the farmer's decision not to use a concentration of carbon dioxide higher than 0.08%.
✅ Correct Answers (Any two from)
- Rate does not increase: It would not increase/change the rate of photosynthesis (the rate stays at 20 arbitrary units).
- Growth/yield does not increase: It would not increase the yield / growth of tomatoes.
- Economic cost: It would cost more money / extra gas is expensive / profit will not increase (waste of money).
- Also allowed: Avoiding unnecessary carbon emissions / global warming.
💡 Limiting Factors in Commercial Farming
Look at Table 2 above 0.08%:
- At 0.08%: Rate = 20
- At 0.10%: Rate = 20
- At 0.12%: Rate = 20
Above 0.08%, carbon dioxide is no longer the limiting factor. Light intensity or temperature is now limiting the rate. Spending money to pump in more CO₂ gives zero extra tomato crop!
❌ Common Errors
- Vague statements like "it damages the plant" (CO₂ at 0.10% is not toxic to tomato plants).
- Saying "the rate decreases" (look closely at the table—it levels off at 20, it does not drop!).
- Failing to mention the cost/money/profit aspect when the question explicitly asks about a commercial farmer.
Topics
Biology · B4: Bioenergetics
Question and mark scheme from the AQA GCSE Biology examination, Biology Paper 1 (Foundation), June 2022. QuestionVault is an independent revision resource; questions remain the copyright of the awarding body.