AQA GCSE Biology Biology Paper 1 (Foundation), June 2022: Question 3

8 marks · Standard Demand difficulty · Short Answer

Analyse data on the effect of carbon dioxide concentration on the rate of photosynthesis in tomato plants, identify control variables, and explain limiting factors.

Practise this question

Question

Question 3 presents Table 2 showing percentage concentration of carbon dioxide in air (from 0.00% to 0.12% in 0.02% increments) and the corresponding rate of photosynthesis in arbitrary units (0, 5, 16, 19, 20, 20, 20). It contains five sub-questions: 03.1 asks for two control variables (2 marks); 03.2 is a multiple choice question asking which carbon dioxide range caused the rate of photosynthesis to change the most (1 mark); 03.3 asks how to improve the validity of the results with three tick-box options (1 mark); 03.4 asks to explain the change in rate between 0.00% and 0.08% (2 marks); and 03.5 asks for two reasons why a farmer decided not to use a carbon dioxide concentration higher than 0.08% (2 marks).
Question text

03 A scientist investigated the rate of photosynthesis of one type of tomato plant.

The tomato plants were grown in a greenhouse.

Table 2 shows the results.

Table 2

Percentage (%)

Rate of photosynthesis

concentration of

in arbitrary units

carbon dioxide in the air

0.00 0

0.02 5

0.04 16

0.06 19

0.08 20

0.10 20

0.12 20

03.1 Give two control variables the scientist should have used in the investigation.

[2 marks]

03.2 Which range of carbon dioxide concentrations caused the rate of photosynthesis to

change the most?

[1 mark]

Tick ( ) one box.

From 0.00% to 0.02%

From 0.02% to 0.04%

From 0.04% to 0.06%

From 0.06% to 0.08% 11

03.3 How could the scientist have improved the validity of the results?

[1 mark]

Tick ( ) one box.

Repeat each reading three times and calculate a mean.

Use concentrations of carbon dioxide above 0.12%.

Use different tomato plants for each concentration.

03.4 Explain the change in the rate of photosynthesis when the concentration of

carbon dioxide increased between 0.00% to 0.08%.

[2 marks]

03.5 A farmer decided not to use a concentration of carbon dioxide higher than 0.08%

to grow tomato plants.

Suggest two reasons for the farmer’s decision.

Use information from Table 2 and your own knowledge.

[2 marks]

Mark scheme

Show the mark scheme Mark scheme for Question 3 with answers, extra information, marks, and AO/spec references. 03.1 allows temperature, plant size/leaves, light, water volume, or fertiliser (2 marks, AO3). 03.2 answer is 'from 0.02% to 0.04%' (1 mark, AO3). 03.3 answer is 'repeat each reading three times and calculate a mean' (1 mark, AO3). 03.4 requires (the rate of photosynthesis) increases (1 mark) because carbon dioxide is needed for photosynthesis (1 mark, AO2). 03.5 awards 2 marks for any two of: would not increase the rate of photosynthesis/growth, or it would cost more / waste money (AO3). Total: 8 marks.

Question 3

AO /

Question Answers Extra information Mark

Spec. Ref.

03.1 any two from: 2 AO3

4.4.1.2

• temperature RPA6

• size of tomato plants or size / allow age of plant

number of leaves

• light

• (volume of) water allow (amount of) water

• (amount / type of) fertiliser / allow (type of) compost / soil

minerals / ions / nutrients allow named example of mineral

(given to plants) ion such as nitrate / magnesium

• time before rate readings are ignore time unqualified

taken

ignore type of tomato plant

ignore type of greenhouse

AO /

Spec. Ref.

03.2 from 0.02% to 0.04% 1 AO3

4.4.1.2

AO /

Spec. Ref.

03.3 repeat each reading three times 1 AO3

and calculate a mean 4.4.1.2

RPA6

AO /

Spec. Ref.

03.4 (the rate of photosynthesis) ignore values 1 AO2

increases 4.4.1.1

4.4.1.2

(because) carbon dioxide is 1

needed for photosynthesis

allow 2 marks for (there is) more

carbon dioxide for (more)

photosynthesis

AO /

Spec. Ref.

03.5 any two from: 2 AO3

4.4.1.2

• it would not increase the rate allow it would not change the

(of photosynthesis) rate (of photosynthesis)

allow photosynthesis would not

increase

• it would not increase the

growth of tomatoes

• it would cost more allow idea of profit will not

increase

allow reference to avoiding

global warming

Total Question 3 8

How to answer it

Photosynthesis & Limiting Factors: Tomato Plant Investigation

📌 What this question tests

This question assesses your understanding of AQA Biology Topic 4.4 (Bioenergetics). You need to identify control variables to ensure a fair test, interpret rate data from a table, select methods to increase experimental validity, explain the biological effect of a limiting reactant (CO₂), and evaluate the economic costs and benefits for commercial crop growers.

Question 03.1 • 2 Marks

Control Variables in Plant Experiments

Give two control variables the scientist should have used in the investigation.

✅ Correct Answers (Choose any two)

  • Temperature
  • Light intensity (or light wavelength / duration)
  • Volume of water (or amount of water)
  • Size / age of tomato plants (or number/surface area of leaves)
  • Amount / type of fertiliser / mineral ions (e.g. nitrate, magnesium) or compost type
  • Time before rate readings are recorded

🧠 Exam Technique

Read the question stem carefully: it already states "one type of tomato plant" and that they are in a "greenhouse". Never give a variable that has already been explicitly stated as fixed in the text!

❌ Common Errors

  • Writing just "time" unqualified (you must state time before readings are taken or duration of exposure).
  • Writing "type of tomato plant" (already controlled in the question stem).
  • Writing "greenhouse" (not a measurable variable).
  • Stating "carbon dioxide" (this is the independent variable, not a control variable!).
Question 03.2 • 1 Mark

Analysing Changes in Rate from Table Data

Which range of carbon dioxide concentrations caused the rate of photosynthesis to change the most?

✅ Correct Answer

[✓] From 0.02% to 0.04%

Award 1 mark for the correct box ticked.

📐 Step-by-Step Data Check

Calculate the difference (increase in rate) across each interval:

  • 0.00% → 0.02%: 5 − 0 = +5
  • 0.02% → 0.04%: 16 − 5 = +11 (Largest increase!)
  • 0.04% → 0.06%: 19 − 16 = +3
  • 0.06% → 0.08%: 20 − 19 = +1

🧠 Exam Technique

Never rely on a quick glance. Always do quick mental or written subtractions in the margin for each interval given in the options to avoid picking the initial or steepest apparent step by mistake.

Question 03.3 • 1 Mark

Improving Experimental Validity & Repeatability

How could the scientist have improved the validity of the results?

✅ Correct Answer

[✓] Repeat each reading three times and calculate a mean.

Award 1 mark for the correct box ticked.

💡 Key Knowledge: Why Repeats Matter

Repeating measurements and calculating a mean:

  • Allows you to identify and discard anomalies.
  • Reduces the effect of random measurement errors.
  • Increases the reliability and validity of your conclusion.

❌ Why the other choices are wrong

  • "Use concentrations of carbon dioxide above 0.12%": This only extends the range; it does not check whether current values are accurate.
  • "Use different tomato plants for each concentration": This introduces plant-to-plant genetic variation as an uncontrolled variable, which actually reduces validity.
Question 03.4 • 2 Marks

Explaining the Trend: Carbon Dioxide as a Limiting Factor

Explain the change in the rate of photosynthesis when the concentration of carbon dioxide increased between 0.00% to 0.08%.

✅ Correct Marking Points

  • Mark 1 (Observation/Trend): The rate of photosynthesis increases.
  • Mark 2 (Biological Reason): (Because) carbon dioxide is a reactant / needed for photosynthesis (or CO₂ was the limiting factor).
Alternative: Award 2 marks directly for stating: "(there is) more carbon dioxide for (more) photosynthesis".

💡 Chemical Context

Recall the word and chemical equations for photosynthesis:

carbon dioxide + water → glucose + oxygen

6CO₂ + 6H₂O → C₆H₁₂O₆ + 6O₂

Between 0.00% and 0.08%, carbon dioxide is the limiting factor. Providing more reactant molecules allows photosynthesis to proceed faster.

🧠 "Explain" Command Word Strategy

An explain question always needs two parts:

  1. Say what happens: The rate increases.
  2. Say why it happens: Because CO₂ is required as a reactant.

Examiner note: Do not just quote numbers from the table—the mark scheme specifically states "ignore values" here.

Question 03.5 • 2 Marks

Commercial Decisions: Economics vs Plant Yield

Suggest two reasons for the farmer's decision not to use a concentration of carbon dioxide higher than 0.08%.

✅ Correct Answers (Any two from)

  • Rate does not increase: It would not increase/change the rate of photosynthesis (the rate stays at 20 arbitrary units).
  • Growth/yield does not increase: It would not increase the yield / growth of tomatoes.
  • Economic cost: It would cost more money / extra gas is expensive / profit will not increase (waste of money).
  • Also allowed: Avoiding unnecessary carbon emissions / global warming.

💡 Limiting Factors in Commercial Farming

Look at Table 2 above 0.08%:

  • At 0.08%: Rate = 20
  • At 0.10%: Rate = 20
  • At 0.12%: Rate = 20

Above 0.08%, carbon dioxide is no longer the limiting factor. Light intensity or temperature is now limiting the rate. Spending money to pump in more CO₂ gives zero extra tomato crop!

❌ Common Errors

  • Vague statements like "it damages the plant" (CO₂ at 0.10% is not toxic to tomato plants).
  • Saying "the rate decreases" (look closely at the table—it levels off at 20, it does not drop!).
  • Failing to mention the cost/money/profit aspect when the question explicitly asks about a commercial farmer.

Topics

Biology · B4: Bioenergetics

Question and mark scheme from the AQA GCSE Biology examination, Biology Paper 1 (Foundation), June 2022. QuestionVault is an independent revision resource; questions remain the copyright of the awarding body.