AQA GCSE Biology Biology Paper 1 (Higher), June 2022: Question 2

12 marks · Standard Demand difficulty · Extended Answer

Identify cell structures in fungal cells, fungal diseases, calculate division frequency, identify protein digestion and food testing reagents, and evaluate the nutritional value of meat versus meat-free burgers.

Practise this question

Question

Question 2 begins with Figure 2 showing a diagram of a fungal cell with an outer cell wall, cell membrane, cytoplasm, nucleus, and large vacuole. Sub-questions include: 02.1 asks to name a structure present in plant and fungal cells but not animal cells; 02.2 asks to identify a fungal disease from Gonorrhoea, Malaria, Measles, and Rose black spot; 02.3 asks to calculate how many times a fungal cell divides in 24 hours if it divides once every 90 minutes; 02.4 asks where protein is digested in the human digestive system; 02.5 asks which reagent tests for protein; and 02.6 presents Table 3 comparing nutritional contents (protein, fibre, fat, carbohydrate, and cholesterol per 100 g) of meat burgers versus meat-free burgers, asking for a 6-mark evaluation of their role in a healthy, balanced diet.
Question text

02 Plant cells and fungal cells are similar in structure.

Figure 2 shows a fungal cell.

Figure 2

02.1 Name one structure in Figure 2 which is present in both plant cells and fungal cells

but not in animal cells.

[1 mark]

02.2 Which disease is caused by a fungus?

[1 mark]

Tick ( ) one box.

Gonorrhoea

Malaria

Measles

Rose black spot 10

02.3 A fungal cell divides once every 90 minutes.

How many times would this fungal cell divide in 24 hours?

[2 marks]

Number of times cell divides in 24 hours =

Some types of fungal cell are grown to produce high-protein food.

The high-protein food can be used to make meat-free burgers.

02.4 Where is protein digested in the human digestive system?

[1 mark]

Tick ( ) one box.

Large intestine

Liver

Salivary glands

Stomach

02.5 Which chemical could be used to test if the burgers contain protein?

[1 mark]

Tick ( ) one box.

Benedict’s reagent

Biuret reagent

Ethanol

Iodine solution 12

02.6 Table 3 shows some information about burgers made from meat and

meat-free burgers.

Table 3

Mass per 100 g of burger

Burgers made Meat-free

from meat burgers

Protein in g 14.0 9.0

*11* Fibre in g 0.9 5.5

Fat in g 16.0 5.2

Carbohydrate in g 15.5 15.1

Cholesterol in mg 120.0 0.0

Evaluate the use of burgers made from meat compared with meat-free burgers in

providing humans with a healthy, balanced diet.

Use information from Table 3 and your own knowledge.

[6 marks]

Mark scheme

Show the mark scheme Mark scheme for Question 2 gives accepted answers: 02.1 accepts '(cell) wall' (ignoring cellulose) or '(large / permanent) vacuole' (1 mark); 02.2 accepts 'rose black spot' (1 mark); 02.3 awards 1 mark for method (24 × 60 / 90 or 24 / 1.5) and 1 mark for answer 16; 02.4 accepts 'stomach' (1 mark); 02.5 accepts 'biuret reagent' (1 mark); 02.6 provides a 3-level descriptor (1–2, 3–4, 5–6 marks) with indicative content comparing fibre, protein, fat, cholesterol, and carbohydrates, linking them to health effects such as CHD, constipation, growth, obesity, and diabetes.

Question 2

AO /

Question Answers Extra information Mark

Spec. Ref.

02.1 (cell) wall ignore cellulose 1 AO3

4.1.1.1

or 4.1.1.2

(large / permanent) vacuole

AO /

Spec. Ref.

02.2 rose black spot 1 AO1

4.3.1.4

AO /

Spec. Ref.

02.3 24 × 60 1440 1 AO2

90 allow 4.1.1.6

4.1.1.1

or

1.5

16 do not accept if a unit is given 1

AO /

Spec. Ref.

02.4 stomach 1 AO1

4.2.2.1

AO /

Spec. Ref.

02.5 biuret reagent 1 AO1

4.2.2.1

RPA4

AO /

Question Answers Mark

Spec. Ref.

02.6 Level 3: A judgement, strongly linked and logically supported by a 5-6 AO3

sufficient range of correct reasons, is given.

Level 2: Some logically linked reasons are given. There may also 3-4 AO2

be a simple judgement.

Level 1: Relevant points are made. They are not logically linked. 1-2 AO1

No relevant content. 0 4.2.2.1

4.2.2.4

Indicative content 4.2.2.5

4.2.2.6

• meat-free burgers contain more fibre

• aids digestion or prevents constipation

• meat burgers contain more protein

• for growth

• meat burgers contain more fat

• can cause CHD or heart attack or narrowing of arteries

• may lead to needing a stent

• may lead to obesity

• obesity is a risk factor for (type 2) diabetes

• meat burgers contain more cholesterol

• can cause narrowing of arteries or CHD or heart attack

• may lead to needing a stent

• may need to take statins

• both burgers have similar amounts of carbohydrate

• good for providing energy

• no information on vitamins / minerals provided for either burger

• meat burgers require animals to be farmed

• increase in methane in atmosphere

• (methane) contributes to global warming

• meat burgers require animals to be slaughtered

• ethical issues

12 • some people won’t eat meat-free burgers

• (because) some people don’t like the idea of eating fungus

• (because) some people prefer the taste of meat

For Level 2, comparisons and linked reasons using own knowledge

are required.

Total Question 2 12

How to answer it

Fungal Biology, Food Tests & Nutritional Evaluation

📋 What this question tests

This question assesses core knowledge of cell ultrastructure, common plant pathogens, cell division rate calculations, human digestive enzymes and organs, food testing biochemical reagents, and an extended evaluation of diet and health using data analysis and biological reasoning.

Question 02.1 • 1 Mark

Cell Ultrastructure Comparison

Identifying shared structures between plant and fungal cells

✅ Correct Answer

Either of the following:

  • (cell) wall
  • (large / permanent) vacuole

💡 Key Knowledge

  • Fungal cells have: nucleus, cytoplasm, cell membrane, mitochondria, ribosomes, a vacuole, and a cell wall (made of chitin, not cellulose).
  • Animal cells have: nucleus, cytoplasm, cell membrane, mitochondria, ribosomes. They lack a cell wall and permanent vacuole.

❌ Common Errors

  • Writing chloroplast: Fungal cells never contain chloroplasts because they do not photosynthesise!
  • The mark scheme notes: ignore "cellulose" (as fungal cell walls are chitin), but writing "cellulose cell wall" can risk losing the mark if contradictions are penalised. Keep it simple: cell wall.

🧠 Exam Technique

Carefully check the diagram provided (Figure 2). You can visibly identify the thick outer boundary (the cell wall), the inner cell membrane, a nucleus, and a large round central vacuole.

Question 02.2 • 1 Mark

Pathogens & Communicable Diseases

Classifying diseases by pathogen type

✅ Correct Answer

Tick: Rose black spot

💡 Key Knowledge: GCSE Pathogen Recall

  • Gonorrhoea: Caused by a bacterium (STD).
  • Malaria: Caused by a protist (vector: mosquito).
  • Measles: Caused by a virus (spread by droplets).
  • Rose black spot: Caused by a fungus (spread by water and wind).
Question 02.3 • 2 Marks

Cell Division Rate Calculation

Calculating how many times a cell divides in a 24-hour period

📐 Step-by-Step Calculation

  1. Step 1: Match the time units.
    Convert 24 hours into minutes:
    24 × 60 = 1440 minutes
    (Alternatively, convert 90 minutes to hours: 90 ÷ 60 = 1.5 hours)
  2. Step 2: Divide the total time by the division time.
    1440 ÷ 90 = 16
    (or 24 ÷ 1.5 = 16)
  3. Final Answer: 16

❌ Common Errors & Examiner Traps

  • Incompatible units: Dividing 90 ÷ 24 or 24 ÷ 90 without converting hours into minutes.
  • Adding units: The mark scheme states: "do not accept if a unit is given" (e.g. writing "16 hours" or "16 minutes" instead of just 16).
  • Confusing number of divisions with final cell count: The question asks how many times it divides (16), not how many cells are produced (2¹⁶).
Mark Breakdown:
• [1 mark] for correct working: (24 × 60) ÷ 90 or 24 ÷ 1.5
• [1 mark] for the correct final number: 16
Question 02.4 & 02.5 • 2 Marks

Digestive System & Food Testing

Protein digestion sites and biochemical food tests

✅ 02.4: Where is protein digested?

Tick: Stomach

Protease (specifically pepsin) is produced and works in the stomach (under acidic conditions, pH 2) and also in the small intestine.

✅ 02.5: Test for protein

Tick: Biuret reagent

Biuret turns from pale blue to purple/lilac in the presence of protein.

💡 Quick Recall of Food Test Reagents

  • Sugars (reducing): Benedict's reagent (heated in water bath → turns green/yellow/brick-red).
  • Starch: Iodine solution (turns from orange-brown to blue-black).
  • Lipids (fats): Ethanol (forms a white cloudy emulsion).
  • Protein: Biuret reagent (turns purple).

❌ Common Misconceptions

  • Thinking the liver digests food: The liver produces bile to emulsify fats and neutralise stomach acid, but digestion of food molecules does not happen inside the liver.
  • Thinking the large intestine digests protein: Only water absorption takes place in the large intestine.
Question 02.6 • 6 Marks

Evaluation: Meat vs. Meat-Free Burgers

Evaluating diet and health using provided table data and own biological knowledge

🧠 How to Get 5–6 Marks (Level 3 Criteria)

To reach Level 3, you must:

  • Compare both types of burgers across several nutrients using data from Table 3.
  • Give linked explanations using your own knowledge (e.g., why excess fat or cholesterol is bad, why fibre is good).
  • Give a justified conclusion/judgement based on your points.
Nutrient Meat Burger Meat-Free Burger Biological Importance / Health Impact (Linked Reason)
Protein 14.0 g (Higher) 9.0 g (Lower) Protein is needed for growth and tissue repair. Meat burger provides more.
Fibre 0.9 g (Lower) 5.5 g (Higher) Fibre aids peristalsis / digestion and prevents constipation. Meat-free is better.
Fat 16.0 g (Higher) 5.2 g (Lower) Excess fat causes weight gain, obesity, and increases risk of Type 2 diabetes and CHD. Meat-free has far less fat.
Cholesterol 120.0 mg (High) 0.0 mg (None) Cholesterol causes atherosclerosis (narrowing of coronary arteries) leading to heart attacks / CHD. Meat-free has none.
Carbohydrate 15.5 g 15.1 g Similar in both — both provide an equal amount of energy.

Model Paragraph Structure

1. Advantages of Meat-Free:
"Meat-free burgers have much less fat (5.2 g vs 16.0 g) and zero cholesterol compared to 120 mg in meat. High fat and cholesterol can build up fatty deposits in coronary arteries, causing coronary heart disease (CHD) or heart attacks. Meat-free also has more fibre (5.5 g vs 0.9 g), which aids digestion and prevents constipation."

2. Advantages of Meat Burgers:
"However, meat burgers contain more protein (14.0 g vs 9.0 g), which is essential for muscle growth and repair."

3. Missing Info & Overall Judgement:
"Both have similar carbohydrate levels for energy (around 15 g), but Table 3 lacks data on vitamins and minerals (such as iron). Overall, meat-free burgers are healthier for a balanced diet because they drastically reduce the risk of heart disease and obesity while still providing adequate protein."

❌ Common Errors in 6-Mark Questions

  • Data copying without biology: Simply quoting "Meat has 16g fat and meat-free has 5.2g" without explaining why fat matters to health. Level 1 only!
  • One-sided answers: Only praising meat-free burgers and completely ignoring the higher protein in meat burgers.
  • No conclusion: Forgetting to give a final reasoned judgement on which is better for a balanced diet.

Topics

Biology · Required Practicals · B1: Cell Biology · B2: Organisation · B3: Infection and Response · Required Practicals

Question and mark scheme from the AQA GCSE Biology examination, Biology Paper 1 (Higher), June 2022. QuestionVault is an independent revision resource; questions remain the copyright of the awarding body.