AQA GCSE Biology Biology Paper 2 (Foundation), June 2022: Question 4

10 marks · Standard Demand difficulty · Short Answer

Analyze the ecological effects of untreated sewage entering a river using population curves and indicator species data.

Practise this question

Question

Question 4 features Figure 6, a line graph showing the number of bacteria, protozoa, and green algae as distance down a river increases, with markers where sewage entered the river, Site A, and Site B. Below are questions 04.1 to 04.4 asking to identify the organism that increases most rapidly, evidence that protozoa feed on bacteria, the process using oxygen, and why oxygen increases with green algae. Then Table 2 provides counts of five animal species at Sites A and B, followed by an incomplete grouped bar chart (Figure 7) where students must plot bars for sludge worm and bloodworm in 04.5, and give evidence regarding oxygen levels in 04.6.
Question text

04 Rivers are sometimes polluted with untreated sewage.

Figure 6 shows some changes that occurred when untreated sewage entered a river.

Figure 6

04.1 Which type of organism had the most rapid increase in numbers when sewage

entered the river?

[1 mark]

Tick ( ) one box.

Bacteria

Green algae

Protozoa 15

04.2 Protozoa are single-celled organisms.

Describe two ways Figure 6 shows that the protozoa in the river feed on bacteria.

[2 marks]

04.3 When sewage enters a river, the concentration of dissolved oxygen decreases.

The decrease in oxygen concentration is caused by organisms in the water.

What process in living organisms uses oxygen?

[1 mark]

04.4 As the numbers of green algae in the river increase, the concentration of dissolved

oxygen increases.

Explain why the concentration of dissolved oxygen increases.

[2 marks]

Scientists counted the numbers of five different animals in the river at sites A and B,

shown in Figure 6 on page 14.

Table 2 shows the results.

Table 2

Number of animals

Animal

Site A Site B

Sludge worm 80 2

Bloodworm 36 8

Water louse 10 55

Freshwater shrimp 5 75

Mayfly nymph 0 15

Figure 7 shows some of the data from Table 2.

Figure 7

04.5 Complete Figure 7.

You should use data from Table 2 for the sludge worm and the bloodworm.

*16* [2 marks]

04.6 The concentration of oxygen in the water at site A is much lower than at site B.

• Sludge worms live in places which have a low concentration of oxygen.

• Mayfly nymphs need a high concentration of oxygen.

Give evidence from Table 2 for the difference in oxygen concentration at

sites A and B.

Refer to sludge worms and to mayfly nymphs in your answer.

[2 marks]

Mark scheme

Show the mark scheme Mark scheme for Question 4 outlines the criteria for parts 04.1 through 04.6: 04.1 accepts bacteria (1 mark); 04.2 accepts bacteria increasing before protozoa or protozoa decreasing as bacteria decrease (2 marks); 04.3 accepts aerobic respiration (1 mark); 04.4 credits algae photosynthesising and releasing oxygen (2 marks); 04.5 awards 1 mark for correct bar heights and 1 mark for correct shading/key (2 marks); 04.6 awards marks for comparative statements showing more sludge worms at A than B and no mayfly nymphs at A compared to B (2 marks). Total = 10 marks.

Question 4

AO /

Question Answers Extra information Mark

Spec. Ref.

04.1 bacteria 1 AO3

4.7.1.2

4.7.3.2

AO /

Spec. Ref.

04.2 any two from: 2 AO3

4.7.1.1

• bacteria increase before allow protozoa increase after 4.7.1.3

protozoa increase bacteria increase 4.7.3.2

or

when bacteria are high,

protozoa increase

• as protozoa increase, bacteria

decrease

• (after site A) as bacteria allow when bacteria are low,

decrease, protozoa also protozoa are low

decrease

AO /

Spec. Ref.

04.3 (aerobic) respiration do not accept anaerobic 1 AO1

respiration 4.7.1.2

4.7.3.2

4.4.2.1

AO /

Spec. Ref.

04.4 (algae carry out) photosynthesis 1 AO2

4.7.3.2

(which) produces oxygen allow algae produce oxygen 1 4.4.1.1

AO /

Spec. Ref.

04.5 bars plotted correctly allow a tolerance of ± ½ a small 1 AO2

square 4.7.1.2

ignore column widths 4.7.3.2

suitable shading 1

AO /

Spec. Ref.

answers must be comparative

04.6 AO3

more sludge worms at A (than allow fewer sludge worms at B 1 4.7.1.2

at B) (than at A) 4.7.3.2

allow high number of sludge

worms at A and low number at

B

no mayfly nymphs at A and allow more mayfly nymphs at B

mayfly nymphs present at B (than at A)

Total Question 4 10

How to answer it

River Pollution: Sewage, Oxygen Levels & Bioindicator Species

📋 What this question tests

This question assesses your understanding of water pollution by sewage, changes in aquatic communities, cellular processes that consume and release oxygen (aerobic respiration and photosynthesis), interpreting line graphs showing predator-prey relationships, accurate bar chart plotting, and using indicator species to evaluate dissolved oxygen levels.

Question 04.1

Most Rapid Increase After Sewage Discharge

Identifying steep gradients on population line graphs

✅ Correct Answer

Tick (✓) Bacteria

1 Mark: Correct tick placed next to Bacteria.

🧠 Exam Technique

  • "Most rapid increase" means the line with the steepest upward slope immediately following the sewage input point.
  • Look directly at the vertical arrow marked "Sewage entered river". The line for bacteria shoots up almost vertically compared to the other two curves.
Question 04.2

Evidence of Feeding Relationships from Graph Trends

Explaining how Figure 6 shows protozoa feed on bacteria

✅ Correct Answers (Any two from)

  • Bacteria numbers increase before protozoa numbers increase (or protozoa numbers rise as bacteria numbers are high).
  • As the number of protozoa increases, the number of bacteria decreases.
  • After Site A, as bacteria numbers decrease, protozoa numbers also decrease (or when bacteria are low, protozoa are low).
2 Marks: 1 mark for each valid relationship described.

💡 Key Knowledge

  • This is a classic predator-prey curve:
    • Prey (bacteria) multiply first due to rich organic food in sewage.
    • Predator (protozoa) lag behind, then multiply as their food source expands.
    • High predator feeding causes the prey population to crash, which subsequently leads to a crash in predator numbers.

❌ Common Errors

  • Vague statements: Saying "they both go up and down" without linking which happens first.
  • Omitting time lag: Forgetting to mention that the increase in protozoa happens after the bacteria multiply.
Question 04.3

Process Consuming Dissolved Oxygen

Cellular metabolism in aquatic microorganisms

✅ Correct Answer

(aerobic) respiration

1 Mark: Clear naming of respiration.

❌ Common Errors

  • Writing "breathing" (breathing is physical ventilation, not a cellular chemical process).
  • Writing "anaerobic respiration" — the mark scheme explicitly states: do not accept anaerobic respiration because anaerobic means without oxygen!
  • Writing "decomposition" without specifying the process — decomposers break down waste by respiring.
Question 04.4

Increase in Dissolved Oxygen by Green Algae

Linking plant-like organisms to oxygen production

✅ Correct Answer

  • (Green algae carry out) photosynthesis [1 mark]
  • (Photosynthesis) produces oxygen (which dissolves in the water) [1 mark]
2 Marks: 1 mark for identifying photosynthesis + 1 mark for stating it produces/releases oxygen.

🧠 Exam Technique: "Explain why..."

Two-mark explanation structure:

Name the biological process → State what product it releases

Word equation: Carbon dioxide + Water → Glucose + Oxygen

Question 04.5

Completing the Bar Chart (Figure 7)

Plotting paired bar data accurately with a key

📐 Step-by-Step Graph Plotting

  • Scale check: 1 large grid square (10 small subdivisions) = 10 units. Therefore, 1 small square = 1 animal.
  • Sludge worm:
    • Site A (white bar): Draw bar to exactly 80 (8 major lines up).
    • Site B (grey bar): Draw bar to exactly 2 (2 tiny squares up).
  • Bloodworm:
    • Site A (white bar): Draw bar to exactly 36 (3 large squares + 6 small squares up).
    • Site B (grey bar): Draw bar to exactly 8 (8 small squares up, just below the 10 line).
  • Shading: Keep Site A bars clear/white, and shade Site B bars grey to match the key.
Mark 1: All 4 bars plotted within tolerance (±½ small square).
Mark 2: Correct shading applied to match the key (Site A unfilled, Site B shaded).

❌ Common Errors

  • Misreading scale: Plotting 36 at 33 or 38 due to miscounting small squares.
  • Inverted shading: Shading Site A instead of Site B, losing the formatting mark.
  • Drawing single bars: Forgetting to plot both sites for each animal.
Question 04.6

Evidence for Oxygen Difference from Indicator Species

Using comparative data to substantiate biological conclusions

✅ Correct Answer

Must include direct comparisons for both named animals:

  • Sludge worms: There are more sludge worms at Site A than at Site B (or 80 at A compared to only 2 at B) [1 mark].
  • Mayfly nymphs: There are no mayfly nymphs at Site A, but they are present / more at Site B (0 at A compared to 15 at B) [1 mark].
2 Marks: 1 comparative mark for sludge worms + 1 comparative mark for mayfly nymphs.

💡 Key Knowledge: Indicator Species

  • Sludge worms & Bloodworms: Adapted to live in muddy, low-oxygen conditions (heavily polluted water). High numbers indicate low dissolved oxygen.
  • Mayfly nymphs & Freshwater shrimps: Highly sensitive to pollution and require high oxygen levels. Their presence proves clean, well-oxygenated water.

🧠 Exam Technique: Always Be Comparative

  • Examiners insist on comparative language when evaluating two sites.
  • Writing "There are 80 sludge worms at A" is not enough on its own — you must say "There are more sludge worms at A than at B" or state both values explicitly.
  • Make sure you write a sentence for each organism named in the question prompt.

Topics

Biology · B4: Bioenergetics · B7: Ecology

Question and mark scheme from the AQA GCSE Biology examination, Biology Paper 2 (Foundation), June 2022. QuestionVault is an independent revision resource; questions remain the copyright of the awarding body.