AQA GCSE Biology Biology Paper 1 (Foundation), June 2023: Question 3

15 marks · Low Demand difficulty · Short Answer

Answer questions on the equation and role of oxygen in photosynthesis and respiration, and evaluate experiments investigating factors affecting the rate of photosynthesis.

Practise this question

Question

Question 3 consists of 9 sub-questions about photosynthesis and an experiment measuring oxygen production. It starts with completing a word equation from given words in a box, followed by explaining the cellular use of oxygen. It describes a method using pondweed exposed to different coloured light bulbs, with questions on hazard/risk, controlling water temperature, and explaining the need for temperature control. Table 3 presents the number of bubbles produced in 2 minutes for blue (46), green (8), red (38), and yellow (29) light, accompanied by questions identifying the highest rate and the best display format. Finally, Figure 1 shows a linear graph plotting rate of photosynthesis against light intensity up to 5 thousand lux, asking for a description of the relationship.
Question text

03 Photosynthesis produces oxygen.

03.1 Complete the word equation for photosynthesis.

Choose answers from the box.

[3 marks]

carbon dioxide fat glucose

nitrogen protein water

… + … → … + oxygen

03.2 Explain how oxygen is used in cells.

[2 marks]

A student investigated the effect of light from different coloured light bulbs

on photosynthesis.

The student:

• used pondweed in a beaker of water

• used different coloured light bulbs in a lamp

• counted the number of bubbles of oxygen the pondweed produced in 2 minutes

for each colour of light bulb.

03.3 Give one hazard the student would need to consider when using the apparatus in

this investigation.

Give the risk the hazard would cause.

[2 marks]

Hazard

*10* Risk

03.4 The student needed to keep the temperature of the water in the beaker the same

throughout the investigation.

Describe how the student could keep the temperature of the water the same.

[1 mark]

03.5 The beaker of water contained the pondweed.

Explain why the temperature of the water in the beaker needed to be kept the same

throughout the investigation.

[2 marks]

Table 3 shows the results.

Table 3

Number of bubbles of oxygen

Colour of light bulb

produced in 2 minutes

Blue 46

Green 8

Red 38

Yellow 29

03.6 Which colour of light caused the highest rate of photosynthesis in the pondweed?

[1 mark]

Tick ( ) one box.

Blue

Green

Red

Yellow

03.7 What is the best way to display the data in Table 3?

[1 mark]

Tick ( ) one box.

Bar graph

Line graph

Scatter graph 13

03.8 The student wanted to measure the volume of oxygen the pondweed produced

in 2 minutes.

*12* Name one piece of apparatus the student could use to measure the

volume of oxygen.

[1 mark]

03.9 Another student investigated the effect of light intensity on the rate of photosynthesis.

Figure 1 shows the results.

Figure 1

Describe what Figure 1 shows about the relationship between light intensity and the

rate of photosynthesis.

[2 marks]

Mark scheme

Show the mark scheme The mark scheme provides answers for questions 03.1 to 03.9, totalling 15 marks. 03.1 credits carbon dioxide, water, and glucose (3 marks). 03.2 requires aerobic respiration and releasing/transferring energy (2 marks). 03.3 awards 2 marks for a matched hazard and risk pair (e.g., hot lamp causing burn, broken glass causing cuts, electricity near water causing shock). 03.4 accepts water bath or heat shield (1 mark). 03.5 explains temperature as a limiting factor affecting enzyme activity and changing the bubble count (2 marks). 03.6 is blue (1 mark). 03.7 is bar graph (1 mark). 03.8 accepts measuring cylinder, gas syringe, or burette (1 mark). 03.9 awards 2 marks for stating rate increases as light intensity increases and that it increases linearly/directly proportionally.

Question 3

AO /

Question Answers Extra information Mark

Spec Ref.

03.1 (lhs) allow word(s) take precedence AO1

over formulae 4.4.1.1

carbon dioxide 1

in either order

water 1

(rhs)

glucose 1

AO /

Spec Ref.

03.2 (used in aerobic) respiration do not accept anaerobic 1 AO1

4.4.1.2

to release / transfer energy allow for energy 1 4.4.2.1

allow (respiration) by

mitochondria

do not accept energy created /

made / produced

AO /

Spec Ref.

03.3 mark in pairs AO2

4.4.1.2

any one pair from:

RPA6

• bulb / lamp is hot 1

(so) may burn you / skin 1

• glassware is breakable (1)

(so) may cut skin (1)

• electricity in close proximity to allow named example of

water (1) electrical item in close proximity

to water

(so) may get electric shock (1)

• scissors / scalpel (to cut

pondweed) are sharp (1)

may cut skin (1) 11

AO /

Spec Ref.

03.4 (use a) water bath allow put beaker into (another) 1 AO2

beaker of water 4.4.1.2

RPA6

allow other correct methods eg

heat shield

allow use an LED

AO /

Spec Ref.

03.5 (change in) temperature affects / allow temperature is a limiting 1 AO2

changes (the rate of) factor (of photosynthesis)

photosynthesis

allow temperature affects

enzyme (activity)

allow temperature affects rate of

reaction

(so) will affect / change the allow (so) will affect results 1 AO3

number of bubbles (produced) 4.4.1.2

allow (so) will affect validity

RPA6

12 ignore to make it fair

ignore so the results will be the

same

AO /

Spec Ref.

03.6 blue 1 AO3

4.4.1.2

RPA6

AO /

Spec Ref.

03.7 bar graph 1 AO3

4.4.1.2

RPA6

AO /

Spec Ref.

03.8 any one from: 1 AO3

4.4.1.2

• measuring cylinder

RPA6

• (gas) syringe

• burette

AO /

Spec Ref.

03.9 as light (intensity) increases, allow positive correlation 1 AO3

rate / photosynthesis increases 4.4.1.2

(rate / photosynthesis increases) allow rate / photosynthesis 1

at the same rate as light increases linearly / evenly

(intensity) allow description of shape of line

eg straight / linear 13

allow rate is (directly)

proportional to light (intensity)

for 2 marks

Total Question 3 15

How to answer it

Photosynthesis: Equation, Cellular Use & Required Practical Investigation

📋 What this question tests

This 15-mark question assesses fundamental recall and practical skills from AQA Biology Topic 4 (Bioenergetics) and Required Practical 6 (Investigating Photosynthesis):

  • Chemical Equation: Recalling reactants and products for photosynthesis.
  • Cell Biology Link: Explaining the cellular role of oxygen in aerobic respiration.
  • Working Scientifically (Lab Safety & Apparatus): Identifying hazards vs risks, controlling variables, and choosing appropriate gas-measuring equipment.
  • Data Handling: Interpreting tables, selecting the correct chart type for categorical data, and describing linear relationships on a graph.
Part 03.1 • Word Equation

Completing the Photosynthesis Word Equation

3 marks • AO1 recall

✅ Correct Answer

carbon dioxide + water → glucose + oxygen

  • Left side: carbon dioxide [1 mark]
  • Left side: water [1 mark] (either order on LHS)
  • Right side: glucose [1 mark]

❌ Common Errors

  • Writing chemical formulas instead of words when words are provided in the box (e.g., writing CO₂ instead of selecting from the given words).
  • Confusing photosynthesis with respiration and putting glucose on the left.
  • Guessing unrelated nutrients like protein or fat.
Part 03.2 • Cellular Function

How Oxygen Is Used in Cells

2 marks • AO1 recall & explanation

✅ Mark Scheme Breakdown

  • Mark 1: Used in (aerobic) respiration.
  • Mark 2: To release energy (or transfer energy / for energy).

❌ Examiner Traps to Avoid

  • "Energy is made / created / produced": Never say energy is created! The law of conservation of energy applies. Say energy is released or transferred.
  • Do not write anaerobic respiration — oxygen is required specifically for aerobic respiration in the mitochondria.
Part 03.3 • Health & Safety

Hazard and Associated Risk

2 marks • AO2 practical application (marked in pairs)

✅ Acceptable Hazard & Risk Pairs (Choose ONE)

  • Hazard: Lamp / bulb gets hot
    Risk: May burn skin / cause a burn.
  • Hazard: Glassware / beaker is breakable
    Risk: May cut skin.
  • Hazard: Water near electricity / electrical wires
    Risk: Electric shock.
  • Hazard: Scalpel / scissors (to cut pondweed)
    Risk: May cut skin.

🧠 Exam Technique: Hazard vs Risk

Make sure you understand the difference:

  • Hazard: The object or situation with potential to cause harm (e.g. hot bulb).
  • Risk: The injury or damage that could result (e.g. burning fingers).

These marks are awarded in pairs; the risk must match your stated hazard.

Parts 03.4 & 03.5 • Control Variables

Controlling Water Temperature

1 mark (03.4) & 2 marks (03.5) • AO2 / AO3

✅ 03.4 How to Keep Temperature Constant

Give any one practical method [1 mark]:

  • Place the beaker in a water bath.
  • Put the beaker inside a larger beaker of water (heat buffer).
  • Use a heat shield / clear plastic/glass screen between the lamp and beaker.
  • Use an LED bulb (which emits very little heat).

✅ 03.5 Why Temperature Must Be Controlled

  • Mark 1: Temperature affects the rate of photosynthesis / temperature is a limiting factor / temperature affects enzyme activity.
  • Mark 2: Therefore it would change the number of bubbles / affect the results / invalidate the experiment.

❌ Common Misconception: "To make it a fair test"

Writing solely "to make it a fair test" scores 0 marks at GCSE. Always explain the underlying biological reason: temperature affects enzyme activity and reaction rate, which would distort the effect of the light colour.

Parts 03.6 & 03.7 • Data Interpretation

Analysing the Results Table

1 mark each • AO3

✅ 03.6 Highest Photosynthesis Rate

Answer: Blue [1 mark]

From Table 3, blue light produced 46 bubbles in 2 minutes, which is higher than red (38), yellow (29), or green (8).

✅ 03.7 Best Way to Display Data

Answer: Bar graph [1 mark]

  • The independent variable (colour of light) is categoric / discrete data (distinct categories, not continuous numbers).
  • Line graphs and scatter graphs require continuous numerical data on both axes.
Part 03.8 • Apparatus Precision

Measuring the Volume of Oxygen

1 mark • AO3 practical skills

✅ Correct Equipment (Any ONE)

  • (Gas) syringe [1 mark]
  • Measuring cylinder (inverted over water) [1 mark]
  • Burette [1 mark]

🧠 Exam Technique: Volume vs Rate

Counting bubbles only tells you the number of bubbles, not their size or volume. To quantify volume precisely, apparatus with calibrated graduation marks is essential. A beaker or boiling tube alone cannot measure volume accurately.

Part 03.9 • Graph Description

Describing the Relationship from Figure 1

2 marks • AO3 data analysis

✅ Mark Scheme Requirements

  • Mark 1: As light intensity increases, the rate of photosynthesis increases (positive correlation).
  • Mark 2: The increase is at a constant rate / linear / a straight line through the origin.
  • Shortcut: Stating that rate is "directly proportional" to light intensity secures both marks immediately!

🧠 What Top Students Do

A complete description of a graph has two parts:

  1. The overall trend (increases as X increases).
  2. The pattern or proportionality (linear, directly proportional, or levels off). Notice on Figure 1 that the line goes straight from (0,0) to (5,16) without leveling off!
Total for Question 3: 15 marks

Topics

Biology · Required Practicals · B4: Bioenergetics · Required Practicals

Question and mark scheme from the AQA GCSE Biology examination, Biology Paper 1 (Foundation), June 2023. QuestionVault is an independent revision resource; questions remain the copyright of the awarding body.