AQA GCSE Biology Biology Paper 1 (Foundation), June 2023: Question 3
15 marks · Low Demand difficulty · Short Answer
Answer questions on the equation and role of oxygen in photosynthesis and respiration, and evaluate experiments investigating factors affecting the rate of photosynthesis.
Practise this questionQuestion
Question text
03 Photosynthesis produces oxygen.
03.1 Complete the word equation for photosynthesis.
Choose answers from the box.
[3 marks]
carbon dioxide fat glucose
nitrogen protein water
… + … → … + oxygen
03.2 Explain how oxygen is used in cells.
[2 marks]
A student investigated the effect of light from different coloured light bulbs
on photosynthesis.
The student:
• used pondweed in a beaker of water
• used different coloured light bulbs in a lamp
• counted the number of bubbles of oxygen the pondweed produced in 2 minutes
for each colour of light bulb.
03.3 Give one hazard the student would need to consider when using the apparatus in
this investigation.
Give the risk the hazard would cause.
[2 marks]
Hazard
*10* Risk
03.4 The student needed to keep the temperature of the water in the beaker the same
throughout the investigation.
Describe how the student could keep the temperature of the water the same.
[1 mark]
03.5 The beaker of water contained the pondweed.
Explain why the temperature of the water in the beaker needed to be kept the same
throughout the investigation.
[2 marks]
Table 3 shows the results.
Table 3
Number of bubbles of oxygen
Colour of light bulb
produced in 2 minutes
Blue 46
Green 8
Red 38
Yellow 29
03.6 Which colour of light caused the highest rate of photosynthesis in the pondweed?
[1 mark]
Tick ( ) one box.
Blue
Green
Red
Yellow
03.7 What is the best way to display the data in Table 3?
[1 mark]
Tick ( ) one box.
Bar graph
Line graph
Scatter graph 13
03.8 The student wanted to measure the volume of oxygen the pondweed produced
in 2 minutes.
*12* Name one piece of apparatus the student could use to measure the
volume of oxygen.
[1 mark]
03.9 Another student investigated the effect of light intensity on the rate of photosynthesis.
Figure 1 shows the results.
Figure 1
Describe what Figure 1 shows about the relationship between light intensity and the
rate of photosynthesis.
[2 marks]
Mark scheme
Show the mark scheme
Question 3
AO /
Question Answers Extra information Mark
Spec Ref.
03.1 (lhs) allow word(s) take precedence AO1
over formulae 4.4.1.1
carbon dioxide 1
in either order
water 1
(rhs)
glucose 1
AO /
Spec Ref.
03.2 (used in aerobic) respiration do not accept anaerobic 1 AO1
4.4.1.2
to release / transfer energy allow for energy 1 4.4.2.1
allow (respiration) by
mitochondria
do not accept energy created /
made / produced
AO /
Spec Ref.
03.3 mark in pairs AO2
4.4.1.2
any one pair from:
RPA6
• bulb / lamp is hot 1
(so) may burn you / skin 1
• glassware is breakable (1)
(so) may cut skin (1)
• electricity in close proximity to allow named example of
water (1) electrical item in close proximity
to water
(so) may get electric shock (1)
• scissors / scalpel (to cut
pondweed) are sharp (1)
may cut skin (1) 11
AO /
Spec Ref.
03.4 (use a) water bath allow put beaker into (another) 1 AO2
beaker of water 4.4.1.2
RPA6
allow other correct methods eg
heat shield
allow use an LED
AO /
Spec Ref.
03.5 (change in) temperature affects / allow temperature is a limiting 1 AO2
changes (the rate of) factor (of photosynthesis)
photosynthesis
allow temperature affects
enzyme (activity)
allow temperature affects rate of
reaction
(so) will affect / change the allow (so) will affect results 1 AO3
number of bubbles (produced) 4.4.1.2
allow (so) will affect validity
RPA6
12 ignore to make it fair
ignore so the results will be the
same
AO /
Spec Ref.
03.6 blue 1 AO3
4.4.1.2
RPA6
AO /
Spec Ref.
03.7 bar graph 1 AO3
4.4.1.2
RPA6
AO /
Spec Ref.
03.8 any one from: 1 AO3
4.4.1.2
• measuring cylinder
RPA6
• (gas) syringe
• burette
AO /
Spec Ref.
03.9 as light (intensity) increases, allow positive correlation 1 AO3
rate / photosynthesis increases 4.4.1.2
(rate / photosynthesis increases) allow rate / photosynthesis 1
at the same rate as light increases linearly / evenly
(intensity) allow description of shape of line
eg straight / linear 13
allow rate is (directly)
proportional to light (intensity)
for 2 marks
Total Question 3 15
How to answer it
Photosynthesis: Equation, Cellular Use & Required Practical Investigation
This 15-mark question assesses fundamental recall and practical skills from AQA Biology Topic 4 (Bioenergetics) and Required Practical 6 (Investigating Photosynthesis):
- Chemical Equation: Recalling reactants and products for photosynthesis.
- Cell Biology Link: Explaining the cellular role of oxygen in aerobic respiration.
- Working Scientifically (Lab Safety & Apparatus): Identifying hazards vs risks, controlling variables, and choosing appropriate gas-measuring equipment.
- Data Handling: Interpreting tables, selecting the correct chart type for categorical data, and describing linear relationships on a graph.
Completing the Photosynthesis Word Equation
3 marks • AO1 recall
✅ Correct Answer
carbon dioxide + water → glucose + oxygen
- Left side: carbon dioxide [1 mark]
- Left side: water [1 mark] (either order on LHS)
- Right side: glucose [1 mark]
❌ Common Errors
- Writing chemical formulas instead of words when words are provided in the box (e.g., writing CO₂ instead of selecting from the given words).
- Confusing photosynthesis with respiration and putting glucose on the left.
- Guessing unrelated nutrients like protein or fat.
How Oxygen Is Used in Cells
2 marks • AO1 recall & explanation
✅ Mark Scheme Breakdown
- Mark 1: Used in (aerobic) respiration.
- Mark 2: To release energy (or transfer energy / for energy).
❌ Examiner Traps to Avoid
- "Energy is made / created / produced": Never say energy is created! The law of conservation of energy applies. Say energy is released or transferred.
- Do not write anaerobic respiration — oxygen is required specifically for aerobic respiration in the mitochondria.
Hazard and Associated Risk
2 marks • AO2 practical application (marked in pairs)
✅ Acceptable Hazard & Risk Pairs (Choose ONE)
- Hazard: Lamp / bulb gets hot
Risk: May burn skin / cause a burn. - Hazard: Glassware / beaker is breakable
Risk: May cut skin. - Hazard: Water near electricity / electrical wires
Risk: Electric shock. - Hazard: Scalpel / scissors (to cut pondweed)
Risk: May cut skin.
🧠 Exam Technique: Hazard vs Risk
Make sure you understand the difference:
- Hazard: The object or situation with potential to cause harm (e.g. hot bulb).
- Risk: The injury or damage that could result (e.g. burning fingers).
These marks are awarded in pairs; the risk must match your stated hazard.
Controlling Water Temperature
1 mark (03.4) & 2 marks (03.5) • AO2 / AO3
✅ 03.4 How to Keep Temperature Constant
Give any one practical method [1 mark]:
- Place the beaker in a water bath.
- Put the beaker inside a larger beaker of water (heat buffer).
- Use a heat shield / clear plastic/glass screen between the lamp and beaker.
- Use an LED bulb (which emits very little heat).
✅ 03.5 Why Temperature Must Be Controlled
- Mark 1: Temperature affects the rate of photosynthesis / temperature is a limiting factor / temperature affects enzyme activity.
- Mark 2: Therefore it would change the number of bubbles / affect the results / invalidate the experiment.
❌ Common Misconception: "To make it a fair test"
Writing solely "to make it a fair test" scores 0 marks at GCSE. Always explain the underlying biological reason: temperature affects enzyme activity and reaction rate, which would distort the effect of the light colour.
Analysing the Results Table
1 mark each • AO3
✅ 03.6 Highest Photosynthesis Rate
Answer: Blue [1 mark]
From Table 3, blue light produced 46 bubbles in 2 minutes, which is higher than red (38), yellow (29), or green (8).
✅ 03.7 Best Way to Display Data
Answer: Bar graph [1 mark]
- The independent variable (colour of light) is categoric / discrete data (distinct categories, not continuous numbers).
- Line graphs and scatter graphs require continuous numerical data on both axes.
Measuring the Volume of Oxygen
1 mark • AO3 practical skills
✅ Correct Equipment (Any ONE)
- (Gas) syringe [1 mark]
- Measuring cylinder (inverted over water) [1 mark]
- Burette [1 mark]
🧠 Exam Technique: Volume vs Rate
Counting bubbles only tells you the number of bubbles, not their size or volume. To quantify volume precisely, apparatus with calibrated graduation marks is essential. A beaker or boiling tube alone cannot measure volume accurately.
Describing the Relationship from Figure 1
2 marks • AO3 data analysis
✅ Mark Scheme Requirements
- Mark 1: As light intensity increases, the rate of photosynthesis increases (positive correlation).
- Mark 2: The increase is at a constant rate / linear / a straight line through the origin.
- Shortcut: Stating that rate is "directly proportional" to light intensity secures both marks immediately!
🧠 What Top Students Do
A complete description of a graph has two parts:
- The overall trend (increases as X increases).
- The pattern or proportionality (linear, directly proportional, or levels off). Notice on Figure 1 that the line goes straight from (0,0) to (5,16) without leveling off!
Topics
Biology · Required Practicals · B4: Bioenergetics · Required Practicals
Question and mark scheme from the AQA GCSE Biology examination, Biology Paper 1 (Foundation), June 2023. QuestionVault is an independent revision resource; questions remain the copyright of the awarding body.