AQA GCSE Biology Biology Paper 2 (Foundation), June 2023: Question 9

15 marks · Standard Demand difficulty · Short Answer

Answer questions on plant cloning, environmental variation, sex determination using a Punnett square, and selective breeding in chickens.

Practise this question

Question

Question 9 consists of nine parts. Parts 09.1 to 09.4 ask about advantages of genetically different versus identical tomato plants, the definition of tissue culture, and why genetically identical plants differ in height. Part 09.5 displays an empty 2x2 Punnett square labelled Female and Male for sex inheritance in dogs, followed by part 09.6 asking why three males and three females are expected from six offspring. Part 09.7 to 09.9 provide Figure 16 containing two bar charts comparing varieties A and B for mean increase in body mass (Growth: A is approx 326 g, B is approx 373 g) and mean number of eggs laid per month (Egg production: A is approx 18.9, B is approx 16.8), followed by questions on control variables, sample size, and selective breeding over generations.
Question text

09 Reproduction can produce offspring which are:

• genetically different

or

• genetically identical.

Farmers grow tomato plants in greenhouses.

The tomatoes are sold in supermarkets.

09.1 Suggest one advantage of growing tomato plants that are genetically different.

[1 mark]

09.2 Suggest one advantage of growing tomato plants that are genetically identical.

[1 mark]

09.3 Scientists can grow genetically identical tomato plants using tissue culture.

What is tissue culture?

[1 mark]

09.4 Genetically identical tomato plants growing in the same garden do not all grow to the

same height.

Give one reason why.

[1 mark]

The sex of dogs is determined by X and Y chromosomes in the same way as

in humans.

09.5 Complete the Punnett square diagram in Figure 15 to show the inheritance of sex

in dogs.

Use the symbols X and Y.

[3 marks]

Figure 15

Female

Male

09.6 A female dog gave birth to six offspring.

Why would you expect there to be three male offspring and three female offspring?

Use your answer to Question 09.5.

[1 mark]

Farmers keep chickens for:

• meat production

• egg production.

Some varieties of chicken grow more quickly and are more suitable for

meat production.

Other varieties of chicken produce more eggs.

A farmer keeps two varieties of chicken, A and B.

The farmer investigated the growth rates and egg-production rates of both varieties.

Figure 16 shows the results.

Figure 16

09.7 Suggest two control variables the farmer should have used in this investigation.

[2 marks]

09.8 Figure 16 shows mean values from 500 chickens of each variety.

Give the reason the farmer used a large number of chickens.

*42* [1 mark]

09.9 The farmer wants to produce a new variety of chicken that is good for both meat

production and egg production.

Describe how selective breeding of chicken varieties A and B can produce the new

variety of chicken.

[4 marks]

Mark scheme

Show the mark scheme Mark scheme for Question 9 detailing answers and marks: 09.1 accepts variation in a named desirable characteristic, not all susceptible to the same disease, or maintaining gene pool (1 mark). 09.2 accepts all having the same desirable characteristic, growing at the same rate, or being ready to harvest at the same time (1 mark). 09.3 accepts growing cells into a new organism (1 mark). 09.4 accepts environmental differences such as water, light, minerals, disease, soil pH, or herbivores (1 mark). 09.5 awards up to 3 marks for correct gametes (male X and Y, female X and X) and offspring genotypes (XX and XY). 09.6 accepts half are XX and half XY, equal probability of X or Y sperm, or 50% chance (1 mark). 09.7 awards 2 marks for control variables such as temperature, food, light, movement space, or time of year. 09.8 awards 1 mark for calculating a valid mean or reducing anomaly effects. 09.9 awards 4 marks for breeding best of A and B, selecting offspring with both high egg numbers and fast growth, breeding these offspring, and repeating over multiple generations.

Question 9

AO /

Question Answers Extra information Mark

Spec. Ref.

09.1 any one from: 1 AO2

• variation of a named / allow eg different flavour / colour 4.6.1.3

described (desirable)

characteristic

• not all susceptible to the

same disease / pathogen

• maintain / increase gene pool

allow different customer

preferences

AO /

Spec. Ref.

09.2 any one from: 1 AO2

• they have the same named allow eg all high yield or all 4.6.1.3

/(desirable) characteristic(s) disease-resistant or same

(desirable) flavour

• they grow at the same rate

• they ready to harvest at same

time

AO /

Spec. Ref.

09.3 (a group of) cells are grown (into ignore clones 1 AO1

a new organism) 4.6.2.5

AO /

Spec. Ref.

09.4 any one from: 1 AO2

4.6.2.1

different 4.7.1.2

• water allow rain

• minerals / ions allow named example

ignore nutrients

• light ignore sun unqualified

• herbivores allow named example

• disease allow named example

• plant density

• soil pH

allow different temperature

allow different environmental

conditions

AO /

Spec. Ref.

09.5 male gametes = X + Y if neither mark awarded, allow 1 AO1

1 mark for male = X + X and

female gametes = X + X female = X + Y 1 AO1

offspring genotypes correctly allow correct for chromosome 1 AO2

derived from gametes assignment in mp1 & mp2

4.6.1.8

AO /

Spec. Ref.

09.6 any one from: 1 AO2

4.6.1.8

• half are XX and half are XY

• equal probability of X or Y

sperm fertilising an egg

• (the Punnett square shows)

50% (chance of) male /

female

AO /

Spec. Ref.

09.7 any two from: 2 AO2

• temperature 4.6.2.3

• type / amount of food allow (volume / amount of) water

• light

• whether chickens are kept

indoors or outdoors 25

• amount of movement / space allow same stocking density

(allowed) allow same number of each type

ignore same number unqualified

• time of year

allow mass at start

allow age of chicken(s)

allow same medication or all

healthy

AO /

Spec. Ref.

09.8 any one from: 1 AO2

4.6.2.3

• (more) valid / representative allow can calculate a valid mean

or

reduce the effect of

anomalies

• (more) accurate mean allow (more) accurate results

allow (more) reliable mean /

results

AO /

Spec. Ref.

09.9 breed best of A and B (together) allow cross / mate best of A and 1 AO2

B

select offspring with highest egg allow select the best offspring 1 AO1

numbers and heaviest / fastest for both desired characteristics

growing

breed (these) offspring together 1 AO1

repeat over many / several do not accept reference to 1 AO1

generations repeated breeding of the original

parents 4.6.2.3

Total Question 9 15

How to answer it

Genetics, Inheritance & Selective Breeding

What this question tests

This exam question evaluates core knowledge from Infection and Response and Inheritance, Variation and Evolution (AQA Biology Topics 4.6 and 4.7):

  • Commercial pros and cons of sexual reproduction (genetic variation) vs asexual reproduction/cloning (genetic uniformity).
  • Definition of tissue culture and factors causing environmental variation in plants.
  • Constructing a Punnett square for sex determination and interpreting probabilities.
  • Scientific practical design: identifying control variables and understanding the purpose of large sample sizes.
  • A structured 4-mark method for selective breeding.
Questions 09.1 & 09.2

Advantages of Genetic Diversity vs Uniformity

Commercial Crop Production in Greenhouses

✅ Correct Answers

09.1 (Genetically Different):

  • Not all plants are susceptible to the same disease / pathogen.
  • Produces variation in characteristics (e.g. flavours, colours) to meet different consumer preferences.
  • Maintains / increases the gene pool.

09.2 (Genetically Identical):

  • All plants share the same desirable trait (e.g. high yield, great taste).
  • They grow at the same rate and are ready to harvest at the exact same time.

❌ Common Errors

  • Vague statements: Saying "they are stronger" or "they taste better" without specifying consistency or variation.
  • Confusing the two: Believing identical plants resist diseases better (the opposite is true: if one gets sick, all are vulnerable).
Examiner Insight: Keep commercial farming in mind. Farmers want identical crops for predictable harvesting and uniform supermarket sales, but want diversity to avoid an entire crop being wiped out by a single infection.
Questions 09.3 & 09.4

Tissue Culture & Environmental Variation

Cloning Techniques and Plant Growth Factors

💡 Key Knowledge

Tissue culture: Growing a new organism using a small group of cells or tissue taken from a parent plant, placed on an agar medium with nutrients and hormones under sterile conditions.

Phenotype = Genotype + Environment: Clones have identical genotypes, but their final appearance (phenotype) is altered by differences in micro-environment.

✅ Accepted Answers

09.3 Definition:

(A group of) cells are grown (into a new plant/organism)

09.4 Reason for variation in height:

  • Different exposure to light / shading.
  • Different amounts of water (or rain).
  • Different amounts of mineral ions (e.g. nitrates/magnesium).
  • Different soil pH, spacing/plant density, or attack by pests/disease.

❌ Mark Scheme Traps

  • 09.3: Writing simply "making clones" is ignored because it does not define the actual process.
  • 09.4: Writing "nutrients" or "sun" without further qualification is often rejected. Always specify minerals/ions and light.
Questions 09.5 & 09.6

Inheritance of Sex

Punnett Square & Probability of Offspring Sex

📐 Completed Punnett Square (09.5)

Female
X X
Male X XX XX
Y XY XY

1 mark: Male gametes (X, Y) | 1 mark: Female gametes (X, X) | 1 mark: 4 correct offspring cells

🧠 Exam Technique (09.6)

Question: Why expect 3 male and 3 female puppies from a litter of 6?

  • State the ratio or percentage shown by the grid: 50% / 1:1 probability.
  • Mention that half of the offspring genotypes are XX (female) and half are XY (male).
  • Alternatively: There is an equal probability of an X sperm or a Y sperm fertilising the egg.
Questions 09.7 & 09.8

Experimental Design: Chicken Investigation

Control Variables and Large Sample Sizes

💡 Control Variables (09.7)

To ensure a valid comparison between chicken varieties A and B, all other conditions must be controlled (choose any two):

  • Type or amount of food / water
  • Housing conditions (indoor vs outdoor temperature, space/stocking density)
  • Light intensity or day length
  • Age or starting mass of the chickens
  • Health status / vaccination history

🧠 Why Sample 500 Chickens? (09.8)

Sample size questions appear frequently across GCSE papers. Memorise these exact phrases:

  • Reduce the effect of anomalies / random variations
  • To calculate a more representative / valid / accurate mean

❌ Common Misconceptions

  • Never say "to prevent anomalies". A large sample does not stop anomalies happening; it reduces their effect on the mean.
  • Avoid simply writing "to make it fair". "Fair test" is KS3 terminology—use valid or accurate.
Question 09.9 (4 Marks)

Selective Breeding Method

Producing a Chicken Good for Both Meat & Eggs

💡 The 4-Step Selective Breeding Recipe

AQA questions on selective breeding almost always follow this 4-step sequence:

  1. Identify & Breed: Select parents from existing stock that show the desired traits and breed them together.
  2. Select Offspring: Choose the best offspring that show a combination of both desired traits.
  3. Breed Offspring: Mate these chosen offspring with each other.
  4. Repeat: Continue this process over many generations until all individuals display the desired characteristics.

✅ Model Answer (Full 4 Marks)

  • Step 1: Cross / breed the best of variety A (high egg production) with the best of variety B (high growth rate / meat) [1 mark].
  • Step 2: From the offspring, select those that produce the highest number of eggs and have the heaviest body mass [1 mark].
  • Step 3: Breed these selected offspring together [1 mark].
  • Step 4: Repeat this process over many generations [1 mark].

❌ What Loses the Final Mark?

Students often write: "Keep repeating by breeding the original parents again."

This gets 0 marks for step 4! You must state that you breed the offspring together and repeat over several / many generations.

Topics

Biology · B6: Inheritance, Variation and Evolution · B7: Ecology

Question and mark scheme from the AQA GCSE Biology examination, Biology Paper 2 (Foundation), June 2023. QuestionVault is an independent revision resource; questions remain the copyright of the awarding body.