AQA GCSE Biology Biology Paper 2 (Foundation), June 2023: Question 9
15 marks · Standard Demand difficulty · Short Answer
Answer questions on plant cloning, environmental variation, sex determination using a Punnett square, and selective breeding in chickens.
Practise this questionQuestion
Question text
09 Reproduction can produce offspring which are:
• genetically different
or
• genetically identical.
Farmers grow tomato plants in greenhouses.
The tomatoes are sold in supermarkets.
09.1 Suggest one advantage of growing tomato plants that are genetically different.
[1 mark]
09.2 Suggest one advantage of growing tomato plants that are genetically identical.
[1 mark]
09.3 Scientists can grow genetically identical tomato plants using tissue culture.
What is tissue culture?
[1 mark]
09.4 Genetically identical tomato plants growing in the same garden do not all grow to the
same height.
Give one reason why.
[1 mark]
The sex of dogs is determined by X and Y chromosomes in the same way as
in humans.
09.5 Complete the Punnett square diagram in Figure 15 to show the inheritance of sex
in dogs.
Use the symbols X and Y.
[3 marks]
Figure 15
Female
Male
09.6 A female dog gave birth to six offspring.
Why would you expect there to be three male offspring and three female offspring?
Use your answer to Question 09.5.
[1 mark]
Farmers keep chickens for:
• meat production
• egg production.
Some varieties of chicken grow more quickly and are more suitable for
meat production.
Other varieties of chicken produce more eggs.
A farmer keeps two varieties of chicken, A and B.
The farmer investigated the growth rates and egg-production rates of both varieties.
Figure 16 shows the results.
Figure 16
09.7 Suggest two control variables the farmer should have used in this investigation.
[2 marks]
09.8 Figure 16 shows mean values from 500 chickens of each variety.
Give the reason the farmer used a large number of chickens.
*42* [1 mark]
09.9 The farmer wants to produce a new variety of chicken that is good for both meat
production and egg production.
Describe how selective breeding of chicken varieties A and B can produce the new
variety of chicken.
[4 marks]
Mark scheme
Show the mark scheme
Question 9
AO /
Question Answers Extra information Mark
Spec. Ref.
09.1 any one from: 1 AO2
• variation of a named / allow eg different flavour / colour 4.6.1.3
described (desirable)
characteristic
• not all susceptible to the
same disease / pathogen
• maintain / increase gene pool
allow different customer
preferences
AO /
Spec. Ref.
09.2 any one from: 1 AO2
• they have the same named allow eg all high yield or all 4.6.1.3
/(desirable) characteristic(s) disease-resistant or same
(desirable) flavour
• they grow at the same rate
• they ready to harvest at same
time
AO /
Spec. Ref.
09.3 (a group of) cells are grown (into ignore clones 1 AO1
a new organism) 4.6.2.5
AO /
Spec. Ref.
09.4 any one from: 1 AO2
4.6.2.1
different 4.7.1.2
• water allow rain
• minerals / ions allow named example
ignore nutrients
• light ignore sun unqualified
• herbivores allow named example
• disease allow named example
• plant density
• soil pH
allow different temperature
allow different environmental
conditions
AO /
Spec. Ref.
09.5 male gametes = X + Y if neither mark awarded, allow 1 AO1
1 mark for male = X + X and
female gametes = X + X female = X + Y 1 AO1
offspring genotypes correctly allow correct for chromosome 1 AO2
derived from gametes assignment in mp1 & mp2
4.6.1.8
AO /
Spec. Ref.
09.6 any one from: 1 AO2
4.6.1.8
• half are XX and half are XY
• equal probability of X or Y
sperm fertilising an egg
• (the Punnett square shows)
50% (chance of) male /
female
AO /
Spec. Ref.
09.7 any two from: 2 AO2
• temperature 4.6.2.3
• type / amount of food allow (volume / amount of) water
• light
• whether chickens are kept
indoors or outdoors 25
• amount of movement / space allow same stocking density
(allowed) allow same number of each type
ignore same number unqualified
• time of year
allow mass at start
allow age of chicken(s)
allow same medication or all
healthy
AO /
Spec. Ref.
09.8 any one from: 1 AO2
4.6.2.3
• (more) valid / representative allow can calculate a valid mean
or
reduce the effect of
anomalies
• (more) accurate mean allow (more) accurate results
allow (more) reliable mean /
results
AO /
Spec. Ref.
09.9 breed best of A and B (together) allow cross / mate best of A and 1 AO2
B
select offspring with highest egg allow select the best offspring 1 AO1
numbers and heaviest / fastest for both desired characteristics
growing
breed (these) offspring together 1 AO1
repeat over many / several do not accept reference to 1 AO1
generations repeated breeding of the original
parents 4.6.2.3
Total Question 9 15
How to answer it
Genetics, Inheritance & Selective Breeding
What this question tests
This exam question evaluates core knowledge from Infection and Response and Inheritance, Variation and Evolution (AQA Biology Topics 4.6 and 4.7):
- Commercial pros and cons of sexual reproduction (genetic variation) vs asexual reproduction/cloning (genetic uniformity).
- Definition of tissue culture and factors causing environmental variation in plants.
- Constructing a Punnett square for sex determination and interpreting probabilities.
- Scientific practical design: identifying control variables and understanding the purpose of large sample sizes.
- A structured 4-mark method for selective breeding.
Advantages of Genetic Diversity vs Uniformity
Commercial Crop Production in Greenhouses
✅ Correct Answers
09.1 (Genetically Different):
- Not all plants are susceptible to the same disease / pathogen.
- Produces variation in characteristics (e.g. flavours, colours) to meet different consumer preferences.
- Maintains / increases the gene pool.
09.2 (Genetically Identical):
- All plants share the same desirable trait (e.g. high yield, great taste).
- They grow at the same rate and are ready to harvest at the exact same time.
❌ Common Errors
- Vague statements: Saying "they are stronger" or "they taste better" without specifying consistency or variation.
- Confusing the two: Believing identical plants resist diseases better (the opposite is true: if one gets sick, all are vulnerable).
Tissue Culture & Environmental Variation
Cloning Techniques and Plant Growth Factors
💡 Key Knowledge
Tissue culture: Growing a new organism using a small group of cells or tissue taken from a parent plant, placed on an agar medium with nutrients and hormones under sterile conditions.
Phenotype = Genotype + Environment: Clones have identical genotypes, but their final appearance (phenotype) is altered by differences in micro-environment.
✅ Accepted Answers
09.3 Definition:
(A group of) cells are grown (into a new plant/organism)
09.4 Reason for variation in height:
- Different exposure to light / shading.
- Different amounts of water (or rain).
- Different amounts of mineral ions (e.g. nitrates/magnesium).
- Different soil pH, spacing/plant density, or attack by pests/disease.
❌ Mark Scheme Traps
- 09.3: Writing simply "making clones" is ignored because it does not define the actual process.
- 09.4: Writing "nutrients" or "sun" without further qualification is often rejected. Always specify minerals/ions and light.
Inheritance of Sex
Punnett Square & Probability of Offspring Sex
📐 Completed Punnett Square (09.5)
| Female | |||
|---|---|---|---|
| X | X | ||
| Male | X | XX | XX |
| Y | XY | XY |
1 mark: Male gametes (X, Y) | 1 mark: Female gametes (X, X) | 1 mark: 4 correct offspring cells
🧠 Exam Technique (09.6)
Question: Why expect 3 male and 3 female puppies from a litter of 6?
- State the ratio or percentage shown by the grid: 50% / 1:1 probability.
- Mention that half of the offspring genotypes are XX (female) and half are XY (male).
- Alternatively: There is an equal probability of an X sperm or a Y sperm fertilising the egg.
Experimental Design: Chicken Investigation
Control Variables and Large Sample Sizes
💡 Control Variables (09.7)
To ensure a valid comparison between chicken varieties A and B, all other conditions must be controlled (choose any two):
- Type or amount of food / water
- Housing conditions (indoor vs outdoor temperature, space/stocking density)
- Light intensity or day length
- Age or starting mass of the chickens
- Health status / vaccination history
🧠 Why Sample 500 Chickens? (09.8)
Sample size questions appear frequently across GCSE papers. Memorise these exact phrases:
- Reduce the effect of anomalies / random variations
- To calculate a more representative / valid / accurate mean
❌ Common Misconceptions
- Never say "to prevent anomalies". A large sample does not stop anomalies happening; it reduces their effect on the mean.
- Avoid simply writing "to make it fair". "Fair test" is KS3 terminology—use valid or accurate.
Selective Breeding Method
Producing a Chicken Good for Both Meat & Eggs
💡 The 4-Step Selective Breeding Recipe
AQA questions on selective breeding almost always follow this 4-step sequence:
- Identify & Breed: Select parents from existing stock that show the desired traits and breed them together.
- Select Offspring: Choose the best offspring that show a combination of both desired traits.
- Breed Offspring: Mate these chosen offspring with each other.
- Repeat: Continue this process over many generations until all individuals display the desired characteristics.
✅ Model Answer (Full 4 Marks)
- Step 1: Cross / breed the best of variety A (high egg production) with the best of variety B (high growth rate / meat) [1 mark].
- Step 2: From the offspring, select those that produce the highest number of eggs and have the heaviest body mass [1 mark].
- Step 3: Breed these selected offspring together [1 mark].
- Step 4: Repeat this process over many generations [1 mark].
❌ What Loses the Final Mark?
Students often write: "Keep repeating by breeding the original parents again."
This gets 0 marks for step 4! You must state that you breed the offspring together and repeat over several / many generations.
Topics
Biology · B6: Inheritance, Variation and Evolution · B7: Ecology
Question and mark scheme from the AQA GCSE Biology examination, Biology Paper 2 (Foundation), June 2023. QuestionVault is an independent revision resource; questions remain the copyright of the awarding body.