AQA GCSE Biology Biology Paper 1 (Higher), 2024: Question 7

17 marks · Standard Demand difficulty · Extended Answer

Explain and interpret investigations into photosynthesis, including factors needed for starch production, chlorophyll deficiency, limiting factors, measurement of oxygen production, and the inverse square law for light intensity.

Practise this question

Question

A multi-part exam question on photosynthesis labelled Question 7, worth 17 marks in total. The first section gives a table of three leaves from the same plant tested after 48 hours: one leaf had both surfaces covered with black paper and showed no starch, one had both surfaces covered and sealed with transparent plastic and showed no starch, and one was not covered and showed starch present; students are asked to explain the results. A second section shows a diagram of a variegated leaf with green and white regions, asks for expected starch-test results for green and white parts, and then asks for explanations, the mineral ion deficiency causing yellow leaves, and the scientific term for yellow leaves. A later section asks how oxygen production could be used to measure rate of photosynthesis, shows a graph of rate of photosynthesis against light intensity with four curves for different carbon dioxide concentrations and temperatures (0.4% CO2 and 25°C, 0.4% CO2 and 15°C, 0.04% CO2 and 25°C, 0.04% CO2 and 15°C), labels point A on the rising part of the highest curve, and asks about limiting factors and the effects of temperature and carbon dioxide concentration. The final part states that light from a source obeys the inverse square law and asks students to tick which expression shows this, with options including light intensity proportional to 1 over distance squared.
Question text

07 A student investigated the effect of different factors on photosynthesis.

The student used three leaves growing on the same plant.

Each leaf was treated in a different way.

After 48 hours the student tested each leaf for starch.

Table 3 shows the results.

Table 3

Leaf tested Treatment Result after 48 hours

Upper and lower surfaces covered

1 No starch present

with black paper

Upper and lower surfaces covered

2 No starch present

and sealed with transparent plastic

3 Not covered Starch present

07.1 Explain the results for the three leaves.

[5 marks]

In another investigation the student used a different type of plant.

The plant was left uncovered in the light for 48 hours.

After 48 hours the student tested a leaf from the plant for starch.

Figure 6 shows the leaf before it was tested for starch.

Figure 6

07.2 Complete Table 4 to show the results you would expect for the starch test on the leaf

in Figure 6.

[1 mark]

Table 4

Part of leaf

Result after 48 hours

tested

Green

White

07.3 Explain the results you gave in Question 07.2.

[2 marks]

In some leaves, the green parts become yellow because of an ion deficiency.

07.4 Which ion is deficient in a plant with yellow leaves?

[1 mark]

07.5 Give the scientific term that describes the yellow colour of the leaves.

[1 mark]

07.6 The rate of photosynthesis is affected by different factors.

How could the oxygen produced during photosynthesis be used to measure

the rate of photosynthesis?

[1 mark]

Light, carbon dioxide and temperature are limiting factors of photosynthesis.

Figure 7 shows how the rate of photosynthesis is affected by light, carbon dioxide

and temperature.

Figure 7

07.7 At point A on Figure 7, light is a limiting factor.

What is meant by a ‘limiting factor’?

[1 mark]

07.8 Explain the effect of increasing temperature and increasing carbon dioxide

concentration on the rate of photosynthesis shown in Figure 7.

[4 marks]

07.9 Photosynthesis investigations often use a light source.

The spreading out of light from a source obeys the inverse square law.

The inverse square law links light intensity to distance from the light source.

Which of the following shows the inverse square law?

[1 mark]

Tick ( ) one box.

light intensity ∝ 2

distance

light intensity ∝ distance2

2 ∝ distance

(light intensity)

2 ∝ 2

(light intensity) distance

Mark scheme

Show the mark scheme The mark scheme for Question 7 is presented in a table with columns for question number, answers, extra information, marks, and AO/specification references. It awards marks for explaining that black paper prevents light so no photosynthesis occurs, transparent plastic prevents carbon dioxide so no photosynthesis occurs, and the uncovered leaf can photosynthesise so glucose and then starch are formed; for the variegated leaf it states starch is present in green parts and absent in white parts because only green parts contain chlorophyll and absorb light. Additional answers include magnesium as the deficient ion, chlorosis as the yellowing term, measuring oxygen volume or bubble count per unit time, defining a limiting factor as something that increases rate when increased or prevents the maximum rate being reached, explaining that increasing temperature and carbon dioxide concentration increase photosynthesis until another factor becomes limiting, and selecting light intensity proportional to 1 over distance squared. The specification references mainly cite 4.4.1.1 and 4.4.1.2 for photosynthesis and RP6 for measuring photosynthesis rate, with 4.3.3.1 for mineral deficiency and chlorosis.

Question 7

AO /

Question Answers Extra information Mark

Spec Ref.

07.1 ignore reference to water

Leaf 1 or covered with black

paper

no light so no photosynthesis ignore reference to carbon 1 AO3

(occurs) dioxide

Leaf 2 or covered with

transparent plastic

no carbon dioxide so no 1 AO3

photosynthesis

Leaf 3 or not covered

light and carbon dioxide present ignore no limiting factors 1 AO3

so leaf can photosynthesise

for either Leaf 1 / 2

(so) glucose not made 1 AO2

allow converse for Leaf 3

(and therefore) glucose / sugar 1 AO2

cannot be converted to starch

4.4.1.1

4.4.1.2

if neither marking points 4 and 5

4.4.1.3

awarded, allow starch

(previously present) has been

broken down for 1 mark

AO /

Spec Ref.

07.2 both required for 1 mark AO3

4.4.1.2

(green) starch / present / allow blue-black / black or 1

positive dark blue

and

(white) no starch or not presentMARKallow yellow / orange / brownSCHEME– – –

or negative

AO /

Spec Ref.

07.3 green part contains chlorophyll ignore chloroplasts 1 AO2

and white part does not

(so) light is absorbed by green allow (so) light is absorbed by 1 AO1

part (but not by white part) so chlorophyll / chloroplasts so

photosynthesis occurs and photosynthesis occurs and 4.4.1.1

starch can be formed starch can be formed 4.4.1.2

allow converse for white part

ignore colours of starch test if

referenced

AO /

Spec Ref.

07.4 magnesium allow Mg / Mg2+ 1 AO1

allow nitrate / iron 4.3.3.1

allow other correct named ions

AO /

Spec Ref.

07.5 chlorosis 1 AO1

4.3.3.1

AO /

Spec Ref.

07.6 allow answers in terms of a AO1

specific time 4.4.1.2

4.4.1.1

(measure the) volume (of ignore measure the amount (of 1 RPA6

oxygen) released / produced in oxygen) released in a given time

a given time

or

(count / number of) bubbles

released / produced in a given MARK SCHEME – – –

time

AO /

Spec Ref.

07.7 (a factor that) if increased would allow answers in terms of 1 AO1

increase the rate (of a reaction) (a) named factor(s) 4.4.1.2

or 4.4.1.1

(a factor that) prevents the rate allow (a factor that) prevents the

(of a reaction) increasing maximum rate (of a reaction)

being reached

AO /

Spec Ref.

07.8 increasing temperature while allow increasing the carbon 1 AO3

keeping the carbon dioxide dioxide (concentration) while 4.4.1.2

(concentration) constant keeping temperature constant 4.4.1.1

increases the rate (of increases the rate (of

photosynthesis) photosynthesis)

increasing the temperature allow increasing the temperature 1

increases the movement of the increases the kinetic energy of

molecules / particles / substrate the molecules / particles /

or substrate

increasing the temperature allow increasing the temperature

increases the rate of enzyme increases the frequency of

activity collisions between molecules /

particles

increasing carbon dioxide 1

concentration increases (the

concentration of) substrate /

reactants

all rates plateau at a certain allow all rates plateau at a 1

point due to another factor being certain point due to chlorophyll

limiting being limiting

do not accept all rates plateau

at a certain point due to light

being limiting

AO /

Spec Ref.

07.9 1 1 AO2

light intensity ∝ 2 4.4.1.2

distance

Total Question 7 17

How to answer it

Photosynthesis Factors and Starch Test

What this question tests

This question checks your ability to explain photosynthesis experiments, interpret leaf test results, identify plant mineral deficiencies, use the idea of limiting factors, and read a simple inverse square law equation. You need short, accurate biology explanations linked to evidence from the question.

Question focus

Photosynthesis investigations: starch test results, chlorophyll and mineral deficiency, oxygen measurements, limiting factors, and light intensity.

Part (a) — Explain the results for the three leaves [5 marks]

✅ Correct answers

  • Leaf 1: covered with black paper, so no light reached the leaf.
  • Without light, no photosynthesis happened.
  • So no glucose was made, and therefore no starch was present.
  • Leaf 2: covered with transparent plastic, so light could get in, but the leaf had no carbon dioxide.
  • Without carbon dioxide, no photosynthesis happened.
  • So no glucose was made and no starch was present.
  • Leaf 3: not covered, so it had light and carbon dioxide.
  • Therefore it could photosynthesise and make glucose, which was stored as starch.

💡 Key knowledge

  • Photosynthesis needs light and carbon dioxide.
  • Starch is made when glucose is produced and then stored.
  • Black paper blocks light completely.
  • Transparent plastic lets light through, but in this question it was used to remove carbon dioxide.

🧠 Exam technique

  • Link each treatment to the missing factor.
  • Then explain the consequence: no photosynthesis → no glucose → no starch.
  • For Leaf 3, make the positive explanation: conditions allow photosynthesis, so starch is present.
  • Use the exact wording “no starch present” or “starch present” for the test result.

❌ Common errors

  • Saying only “it had no water” is not needed and does not score here.
  • Writing “no photosynthesis because no starch” is backwards.
  • Mixing up the roles of light and carbon dioxide.
  • For Leaf 2, some students wrongly said the plastic blocked light. The question says transparent plastic.
Examiner note: full marks needed separate explanations for each leaf. The best answers clearly stated the missing factor, the effect on photosynthesis, and the starch result.

Part (b) — Complete Table 4 for the green and white parts [1 mark]

✅ Correct answers

  • Green part: starch present / positive test / blue-black colour.
  • White part: no starch present / negative test / no blue-black colour.

💡 Key knowledge

  • Green parts contain chlorophyll, so they can photosynthesise.
  • White parts do not contain chlorophyll, so they cannot photosynthesise.

🧠 Exam technique

  • This is a 1-mark table completion question.
  • Write a clear starch test result for each part.

❌ Common errors

  • Giving one answer for both rows.
  • Writing only “yes” or “no” without saying starch.
  • Confusing green with white parts.

Part (c) — Explain the results in Question 07.2 [2 marks]

✅ Correct answer

  • The green part contains chlorophyll and the white part does not.
  • So light is absorbed by the green part, photosynthesis occurs, and starch can be formed.
  • The white part cannot photosynthesise, so no starch is made there.

💡 Key knowledge

  • Chlorophyll is the green pigment needed for photosynthesis.
  • Without chlorophyll, the leaf cannot capture light energy effectively.

🧠 Exam technique

  • You get one mark for chlorophyll in the green part and one for linking that to photosynthesis and starch.
  • Use “because” or “so” to show cause and effect.

❌ Common errors

  • Saying “white areas are covered by chloroplasts” — the mark scheme focuses on chlorophyll.
  • Forgetting to connect chlorophyll to photosynthesis.

Part (d) — Which ion is deficient in yellow leaves? [1 mark]

✅ Correct answer

Magnesium (also allow Mg or Mg²+ ).

💡 Key knowledge

  • Magnesium is needed to make chlorophyll.
  • Without enough magnesium, leaves can become yellow.

❌ Common errors

  • Writing “nitrate” or “iron” when the question specifically asks for yellow leaves.
  • Giving a description instead of the ion name.

Part (e) — Scientific term for the yellow colour of leaves [1 mark]

✅ Correct answer

Chlorosis

💡 Key knowledge

Chlorosis is the yellowing of leaves, often caused by a lack of chlorophyll due to mineral deficiency.

❌ Common errors

  • Writing “necrosis” — that means tissue death, not yellowing.
  • Giving “yellowing” only, without the scientific term.

Part (f) — How could oxygen produced during photosynthesis be used to measure the rate? [1 mark]

✅ Correct answer

Measure the volume of oxygen produced in a given time, or count the number of bubbles produced in a given time.

🧠 Exam technique

  • Always include time in the answer.
  • Examples: “cm³ of oxygen per minute” or “bubbles per minute”.

❌ Common errors

  • Only writing “measure oxygen” — too vague.
  • Leaving out the time, so it is not a rate.

Part (g) — What is a limiting factor? [1 mark]

✅ Correct answer

A factor that, if increased, would increase the rate of photosynthesis, or a factor that stops the rate increasing further.

💡 Key knowledge

  • Limiting factors can include light intensity, carbon dioxide concentration, and temperature.
  • Only one factor limits the rate at a time.

❌ Common errors

  • Saying “anything needed for photosynthesis” — too vague.
  • Not showing that the factor affects the rate.

Part (h) — Explain the effect of increasing temperature and increasing carbon dioxide concentration shown in Figure 7 [4 marks]

✅ Correct answer

  • Increasing the temperature while keeping carbon dioxide concentration constant increases the rate of photosynthesis.
  • This is because higher temperature increases the movement/kinetic energy of molecules and the frequency of collisions, so enzyme activity increases.
  • Increasing the carbon dioxide concentration while keeping temperature constant also increases the rate of photosynthesis.
  • This is because carbon dioxide is a reactant, so more of it increases the rate until another factor becomes limiting.
  • All the curves plateau because another factor becomes limiting.

💡 Key knowledge

  • Photosynthesis is controlled by several limiting factors.
  • Temperature affects enzyme activity.
  • Carbon dioxide is a raw material used in photosynthesis.
  • When the line levels off, something else is limiting the rate.

🧠 Exam technique

  • To get full marks, describe both trends and explain both using biology.
  • Use the graph evidence: higher curves are above lower curves.
  • Finish by stating why the curves level off.

❌ Common errors

  • Saying the plateau happens because “light becomes limiting” in every case — the mark scheme does not accept that here.
  • Forgetting to mention enzyme activity or collisions for temperature.
  • Saying “carbon dioxide is a catalyst” — it is not.

Part (i) — Inverse square law for light intensity [1 mark]

✅ Correct answer

light intensity ∝ 1 / distance²

📐 Calculations / formula reading

  1. Remember the inverse square law means intensity gets smaller very quickly as distance increases.
  2. The correct relationship is:

light intensity ∝ 1 / distance²

❌ Common errors

  • Writing 1 / distance instead of 1 / distance² .
  • Reversing the relationship.
  • Forgetting the square.
Examiner note: this was a simple recall mark, but only the exact inverse square relationship scores.

Top tips to score well on this question

🧠 Exam technique

  • Use the exact science words: chlorophyll, photosynthesis, starch, carbon dioxide, enzyme activity.
  • For explain questions, write in a chain: cause → process → result.
  • When a graph is involved, refer to what happens at low and high values, and mention the plateau.
  • For one-mark answers, keep it short but precise.

💡 Key knowledge

  • Light and carbon dioxide are needed for photosynthesis.
  • Green parts have chlorophyll; white parts do not.
  • Magnesium deficiency causes chlorosis.
  • Rates are often measured per unit time.

❌ Common errors to avoid

  • Mixing up “starch present” with “glucose present”.
  • Forgetting that transparent plastic still lets light through.
  • Giving vague answers like “it changed the rate” without saying how.
  • Missing the square in the inverse square law.

Topics

Biology · Required Practicals · B4: Bioenergetics · Required Practicals

Question and mark scheme from the AQA GCSE Biology examination, Biology Paper 1 (Higher), 2024. QuestionVault is an independent revision resource; questions remain the copyright of the awarding body.