AQA GCSE Biology Biology Paper 1 (Higher), 2024: Question 7
17 marks · Standard Demand difficulty · Extended Answer
Explain and interpret investigations into photosynthesis, including factors needed for starch production, chlorophyll deficiency, limiting factors, measurement of oxygen production, and the inverse square law for light intensity.
Practise this questionQuestion
Question text
07 A student investigated the effect of different factors on photosynthesis.
The student used three leaves growing on the same plant.
Each leaf was treated in a different way.
After 48 hours the student tested each leaf for starch.
Table 3 shows the results.
Table 3
Leaf tested Treatment Result after 48 hours
Upper and lower surfaces covered
1 No starch present
with black paper
Upper and lower surfaces covered
2 No starch present
and sealed with transparent plastic
3 Not covered Starch present
07.1 Explain the results for the three leaves.
[5 marks]
In another investigation the student used a different type of plant.
The plant was left uncovered in the light for 48 hours.
After 48 hours the student tested a leaf from the plant for starch.
Figure 6 shows the leaf before it was tested for starch.
Figure 6
07.2 Complete Table 4 to show the results you would expect for the starch test on the leaf
in Figure 6.
[1 mark]
Table 4
Part of leaf
Result after 48 hours
tested
Green
White
07.3 Explain the results you gave in Question 07.2.
[2 marks]
In some leaves, the green parts become yellow because of an ion deficiency.
07.4 Which ion is deficient in a plant with yellow leaves?
[1 mark]
07.5 Give the scientific term that describes the yellow colour of the leaves.
[1 mark]
07.6 The rate of photosynthesis is affected by different factors.
How could the oxygen produced during photosynthesis be used to measure
the rate of photosynthesis?
[1 mark]
Light, carbon dioxide and temperature are limiting factors of photosynthesis.
Figure 7 shows how the rate of photosynthesis is affected by light, carbon dioxide
and temperature.
Figure 7
07.7 At point A on Figure 7, light is a limiting factor.
What is meant by a ‘limiting factor’?
[1 mark]
07.8 Explain the effect of increasing temperature and increasing carbon dioxide
concentration on the rate of photosynthesis shown in Figure 7.
[4 marks]
07.9 Photosynthesis investigations often use a light source.
The spreading out of light from a source obeys the inverse square law.
The inverse square law links light intensity to distance from the light source.
Which of the following shows the inverse square law?
[1 mark]
Tick ( ) one box.
light intensity ∝ 2
distance
light intensity ∝ distance2
2 ∝ distance
(light intensity)
2 ∝ 2
(light intensity) distance
Mark scheme
Show the mark scheme
Question 7
AO /
Question Answers Extra information Mark
Spec Ref.
07.1 ignore reference to water
Leaf 1 or covered with black
paper
no light so no photosynthesis ignore reference to carbon 1 AO3
(occurs) dioxide
Leaf 2 or covered with
transparent plastic
no carbon dioxide so no 1 AO3
photosynthesis
Leaf 3 or not covered
light and carbon dioxide present ignore no limiting factors 1 AO3
so leaf can photosynthesise
for either Leaf 1 / 2
(so) glucose not made 1 AO2
allow converse for Leaf 3
(and therefore) glucose / sugar 1 AO2
cannot be converted to starch
4.4.1.1
4.4.1.2
if neither marking points 4 and 5
4.4.1.3
awarded, allow starch
(previously present) has been
broken down for 1 mark
AO /
Spec Ref.
07.2 both required for 1 mark AO3
4.4.1.2
(green) starch / present / allow blue-black / black or 1
positive dark blue
and
(white) no starch or not presentMARKallow yellow / orange / brownSCHEME– – –
or negative
AO /
Spec Ref.
07.3 green part contains chlorophyll ignore chloroplasts 1 AO2
and white part does not
(so) light is absorbed by green allow (so) light is absorbed by 1 AO1
part (but not by white part) so chlorophyll / chloroplasts so
photosynthesis occurs and photosynthesis occurs and 4.4.1.1
starch can be formed starch can be formed 4.4.1.2
allow converse for white part
ignore colours of starch test if
referenced
AO /
Spec Ref.
07.4 magnesium allow Mg / Mg2+ 1 AO1
allow nitrate / iron 4.3.3.1
allow other correct named ions
AO /
Spec Ref.
07.5 chlorosis 1 AO1
4.3.3.1
AO /
Spec Ref.
07.6 allow answers in terms of a AO1
specific time 4.4.1.2
4.4.1.1
(measure the) volume (of ignore measure the amount (of 1 RPA6
oxygen) released / produced in oxygen) released in a given time
a given time
or
(count / number of) bubbles
released / produced in a given MARK SCHEME – – –
time
AO /
Spec Ref.
07.7 (a factor that) if increased would allow answers in terms of 1 AO1
increase the rate (of a reaction) (a) named factor(s) 4.4.1.2
or 4.4.1.1
(a factor that) prevents the rate allow (a factor that) prevents the
(of a reaction) increasing maximum rate (of a reaction)
being reached
AO /
Spec Ref.
07.8 increasing temperature while allow increasing the carbon 1 AO3
keeping the carbon dioxide dioxide (concentration) while 4.4.1.2
(concentration) constant keeping temperature constant 4.4.1.1
increases the rate (of increases the rate (of
photosynthesis) photosynthesis)
increasing the temperature allow increasing the temperature 1
increases the movement of the increases the kinetic energy of
molecules / particles / substrate the molecules / particles /
or substrate
increasing the temperature allow increasing the temperature
increases the rate of enzyme increases the frequency of
activity collisions between molecules /
particles
increasing carbon dioxide 1
concentration increases (the
concentration of) substrate /
reactants
all rates plateau at a certain allow all rates plateau at a 1
point due to another factor being certain point due to chlorophyll
limiting being limiting
do not accept all rates plateau
at a certain point due to light
being limiting
AO /
Spec Ref.
07.9 1 1 AO2
light intensity ∝ 2 4.4.1.2
distance
Total Question 7 17
How to answer it
Photosynthesis Factors and Starch Test
This question checks your ability to explain photosynthesis experiments, interpret leaf test results, identify plant mineral deficiencies, use the idea of limiting factors, and read a simple inverse square law equation. You need short, accurate biology explanations linked to evidence from the question.
Question focus
Photosynthesis investigations: starch test results, chlorophyll and mineral deficiency, oxygen measurements, limiting factors, and light intensity.
Part (a) — Explain the results for the three leaves [5 marks]
✅ Correct answers
- Leaf 1: covered with black paper, so no light reached the leaf.
- Without light, no photosynthesis happened.
- So no glucose was made, and therefore no starch was present.
- Leaf 2: covered with transparent plastic, so light could get in, but the leaf had no carbon dioxide.
- Without carbon dioxide, no photosynthesis happened.
- So no glucose was made and no starch was present.
- Leaf 3: not covered, so it had light and carbon dioxide.
- Therefore it could photosynthesise and make glucose, which was stored as starch.
💡 Key knowledge
- Photosynthesis needs light and carbon dioxide.
- Starch is made when glucose is produced and then stored.
- Black paper blocks light completely.
- Transparent plastic lets light through, but in this question it was used to remove carbon dioxide.
🧠 Exam technique
- Link each treatment to the missing factor.
- Then explain the consequence: no photosynthesis → no glucose → no starch.
- For Leaf 3, make the positive explanation: conditions allow photosynthesis, so starch is present.
- Use the exact wording “no starch present” or “starch present” for the test result.
❌ Common errors
- Saying only “it had no water” is not needed and does not score here.
- Writing “no photosynthesis because no starch” is backwards.
- Mixing up the roles of light and carbon dioxide.
- For Leaf 2, some students wrongly said the plastic blocked light. The question says transparent plastic.
Part (b) — Complete Table 4 for the green and white parts [1 mark]
✅ Correct answers
- Green part: starch present / positive test / blue-black colour.
- White part: no starch present / negative test / no blue-black colour.
💡 Key knowledge
- Green parts contain chlorophyll, so they can photosynthesise.
- White parts do not contain chlorophyll, so they cannot photosynthesise.
🧠 Exam technique
- This is a 1-mark table completion question.
- Write a clear starch test result for each part.
❌ Common errors
- Giving one answer for both rows.
- Writing only “yes” or “no” without saying starch.
- Confusing green with white parts.
Part (c) — Explain the results in Question 07.2 [2 marks]
✅ Correct answer
- The green part contains chlorophyll and the white part does not.
- So light is absorbed by the green part, photosynthesis occurs, and starch can be formed.
- The white part cannot photosynthesise, so no starch is made there.
💡 Key knowledge
- Chlorophyll is the green pigment needed for photosynthesis.
- Without chlorophyll, the leaf cannot capture light energy effectively.
🧠 Exam technique
- You get one mark for chlorophyll in the green part and one for linking that to photosynthesis and starch.
- Use “because” or “so” to show cause and effect.
❌ Common errors
- Saying “white areas are covered by chloroplasts” — the mark scheme focuses on chlorophyll.
- Forgetting to connect chlorophyll to photosynthesis.
Part (d) — Which ion is deficient in yellow leaves? [1 mark]
✅ Correct answer
Magnesium (also allow Mg or Mg²+ ).
💡 Key knowledge
- Magnesium is needed to make chlorophyll.
- Without enough magnesium, leaves can become yellow.
❌ Common errors
- Writing “nitrate” or “iron” when the question specifically asks for yellow leaves.
- Giving a description instead of the ion name.
Part (e) — Scientific term for the yellow colour of leaves [1 mark]
✅ Correct answer
Chlorosis
💡 Key knowledge
Chlorosis is the yellowing of leaves, often caused by a lack of chlorophyll due to mineral deficiency.
❌ Common errors
- Writing “necrosis” — that means tissue death, not yellowing.
- Giving “yellowing” only, without the scientific term.
Part (f) — How could oxygen produced during photosynthesis be used to measure the rate? [1 mark]
✅ Correct answer
Measure the volume of oxygen produced in a given time, or count the number of bubbles produced in a given time.
🧠 Exam technique
- Always include time in the answer.
- Examples: “cm³ of oxygen per minute” or “bubbles per minute”.
❌ Common errors
- Only writing “measure oxygen” — too vague.
- Leaving out the time, so it is not a rate.
Part (g) — What is a limiting factor? [1 mark]
✅ Correct answer
A factor that, if increased, would increase the rate of photosynthesis, or a factor that stops the rate increasing further.
💡 Key knowledge
- Limiting factors can include light intensity, carbon dioxide concentration, and temperature.
- Only one factor limits the rate at a time.
❌ Common errors
- Saying “anything needed for photosynthesis” — too vague.
- Not showing that the factor affects the rate.
Part (h) — Explain the effect of increasing temperature and increasing carbon dioxide concentration shown in Figure 7 [4 marks]
✅ Correct answer
- Increasing the temperature while keeping carbon dioxide concentration constant increases the rate of photosynthesis.
- This is because higher temperature increases the movement/kinetic energy of molecules and the frequency of collisions, so enzyme activity increases.
- Increasing the carbon dioxide concentration while keeping temperature constant also increases the rate of photosynthesis.
- This is because carbon dioxide is a reactant, so more of it increases the rate until another factor becomes limiting.
- All the curves plateau because another factor becomes limiting.
💡 Key knowledge
- Photosynthesis is controlled by several limiting factors.
- Temperature affects enzyme activity.
- Carbon dioxide is a raw material used in photosynthesis.
- When the line levels off, something else is limiting the rate.
🧠 Exam technique
- To get full marks, describe both trends and explain both using biology.
- Use the graph evidence: higher curves are above lower curves.
- Finish by stating why the curves level off.
❌ Common errors
- Saying the plateau happens because “light becomes limiting” in every case — the mark scheme does not accept that here.
- Forgetting to mention enzyme activity or collisions for temperature.
- Saying “carbon dioxide is a catalyst” — it is not.
Part (i) — Inverse square law for light intensity [1 mark]
✅ Correct answer
light intensity ∝ 1 / distance²
📐 Calculations / formula reading
- Remember the inverse square law means intensity gets smaller very quickly as distance increases.
- The correct relationship is:
light intensity ∝ 1 / distance²
❌ Common errors
- Writing 1 / distance instead of 1 / distance² .
- Reversing the relationship.
- Forgetting the square.
Top tips to score well on this question
🧠 Exam technique
- Use the exact science words: chlorophyll, photosynthesis, starch, carbon dioxide, enzyme activity.
- For explain questions, write in a chain: cause → process → result.
- When a graph is involved, refer to what happens at low and high values, and mention the plateau.
- For one-mark answers, keep it short but precise.
💡 Key knowledge
- Light and carbon dioxide are needed for photosynthesis.
- Green parts have chlorophyll; white parts do not.
- Magnesium deficiency causes chlorosis.
- Rates are often measured per unit time.
❌ Common errors to avoid
- Mixing up “starch present” with “glucose present”.
- Forgetting that transparent plastic still lets light through.
- Giving vague answers like “it changed the rate” without saying how.
- Missing the square in the inverse square law.
Topics
Biology · Required Practicals · B4: Bioenergetics · Required Practicals
Question and mark scheme from the AQA GCSE Biology examination, Biology Paper 1 (Higher), 2024. QuestionVault is an independent revision resource; questions remain the copyright of the awarding body.