AQA GCSE Biology Biology Paper 2 (Foundation), June 2024: Question 7

9 marks · Standard Demand difficulty · Extended Answer

Analyze an investigation into the effect of chemical Q on root growth in geranium cuttings, calculate concentration differences, and explain why cuttings are preferred over seeds.

Practise this question

Question

Figure 8 displays six test tubes containing geranium stem cuttings left for 10 days in varying concentrations of chemical Q: Tube 1 (0 arbitrary units) shows no roots; Tube 2 (0.01) shows small roots; Tube 3 (0.1) shows extensive root growth; Tube 4 (1) shows fewer roots; Tube 5 (10) shows very small root emergence; Tube 6 (100) shows no roots at all. The questions ask why Tube 1 was used as a control (07.1), to calculate how many times more concentrated chemical Q is in tube 6 than in tube 2 (07.2), to identify the best concentration for root growth via multiple choice (07.3), to provide evidence that high concentrations may be toxic (07.4), and to explain why taking cuttings from plant A is chosen over growing seeds (07.5).
Question text

07 Gardeners can grow plants from:

• seeds

• cuttings taken from adult plants.

A gardener investigated the growth of roots on cuttings from a geranium plant.

This is the method used.

1. Take 6 cuttings from the stems of the same plant.

2. Prepare 6 test tubes, each containing a different concentration of a solution of

chemical Q.

3. Place 1 cutting in each test tube with the cut end of each stem in the solution.

4. Leave the test tubes at room temperature for 10 days.

Figure 8 shows the results.

Figure 8

07.1 Tube 1 contains no chemical Q.

Tube 1 is a control.

Why did the gardener include tube 1 in the investigation?

[1 mark]

*0287*2

. How many times more concentrated is chemical Q in tube 6 than in tube 2?

[2 marks]

Number of times more concentrated =

07.3 What was the best concentration of chemical Q for stimulating root growth?

[1 mark]

Tick ( ) one box.

0.01 arbitrary units

0.1 arbitrary units

1 arbitrary unit

10 arbitrary units

07.4 Give evidence from Figure 8 that a high concentration of chemical Q may be toxic to

geranium plants.

[1 mark]

07.5 The gardener has four types of geranium plant: A, B, C and D.

Plant A produces larger, more brightly-coloured flowers than any of the other plants.

*29* The gardener wants to grow more plants of type A.

Explain why the gardener chooses to take cuttings from plant A instead of growing

seeds from plant A.

[4 marks]

Mark scheme

Show the mark scheme Mark scheme for Question 7: 07.1 awards 1 mark for comparing with other tubes or showing the effect of chemical Q; 07.2 awards 2 marks for 100 / 0.01 = 10 000 / 10^4 times; 07.3 awards 1 mark for 0.1 arbitrary units; 07.4 awards 1 mark for reduced or no root growth at higher concentrations; 07.5 is a 4-mark level of response question with indicative content detailing cuttings being quicker, producing genetically identical clones without variation from gamete fusion or meiosis, and retaining desirable traits like large, brightly-coloured flowers.

Question 7

AO /

Question Answers Extra information Mark

Spec Ref.

07.1 ignore as a control AO2

do not accept control variable 4.6.2.5

to compare (with the other allow to show the difference 1

tubes)

or

to show the effect of chemical Q

AO /

Spec Ref.

07.2 100 1 AO2

0.01 4.6.2.5

10 000 / 104 (times) 1

AO /

Spec Ref.

07.3 0.1 arbitrary units 1 AO3

4.6.2.5

AO /

Spec Ref.

07.4 reduced / no (root) growth at allow no (root) growth in tube 6 / 1 AO3

high(er) concentrations 100 4.6.2.5

allow answer in terms of specific

tubes / concentrations

ignore the higher the

concentration, the fewer roots– – –

grow unqualified

AO /

Question Answers Mark

Spec Ref.

07.5 Level 2: Relevant points (reasons/causes) are identified, given in 3–4 AO2

detail and logically linked to form a clear account.

Level 1: Points are identified and stated simply, but their relevance 1–2 AO3

is not clear and there is no attempt at logical thinking.

No relevant content 0

4.6.1.1

Indicative content 4.6.1.3

4.6.2.1

Taking cuttings: 4.6.2.5

• quicker

o (because) do not have to wait as long for flowers / fruits /

seeds (to form)

• all offspring identical (in appearance) or all have large /

brightly-coloured flowers

o (because) all have the same alleles / genes / DNA

• asexual reproduction or not sexual reproduction or cloning

o (so) no fusing of gametes

o (so) no mixing of alleles / genes from two parents

o (so) offspring are identical (in appearance)

• involves mitosis

o (which) copies genetic material / chromosomes

• does not involve meiosis

o (which) gives different genetic material / chromosomes

• avoids variation in flowers

o due to pollination / cross pollination (from other geraniums)

o (as) no mixing of alleles / genes from two parents

• increased profitability

o less resources needed

o quicker turnover

o consistent quality of plants / flowers

Total Question 7 9

How to answer it

Plant Hormones, Cuttings & Asexual Reproduction

What this question tests

This question assesses experimental design in biology (understanding experimental controls and interpreting concentration effects), quantitative skills (ratio comparisons with decimals), data interpretation from diagrams, and the biological differences between sexual reproduction (seeds) and asexual reproduction / cloning (stem cuttings).

Question 07.1 • 1 Mark

Purpose of an Experimental Control

Explaining why Tube 1 (0 arbitrary units of chemical Q) was set up

✅ Correct Answer

Either of the following:

  • To compare with the other tubes (to see how roots grow without the chemical).
  • To show the effect of chemical Q on root growth.

🧠 Exam Technique

When asked why a "zero concentration" or untreated setup is included, examiners want the word compare or an explicit statement about identifying the effect of the independent variable.

❌ Common Errors

  • Writing "as a control" (simply repeating the question gets 0 marks).
  • Calling it a "control variable" (a control tube is a baseline comparison, not a variable being kept constant).
  • Vague claims like "to make it a fair test".

💡 Key Knowledge

A control experiment provides a baseline measurement. It proves that any observed root growth in tubes 2–6 was caused specifically by chemical Q, rather than just water or room temperature.

Mark allocation: 1 mark for stating that it acts as a comparison or shows the specific effect of chemical Q.
Question 07.2 • 2 Marks

Relative Concentration Calculation

Calculating how many times more concentrated Tube 6 is than Tube 2

📐 Step-by-Step Calculation

  1. Identify values from Figure 8:
    Concentration in Tube 6 = 100 arbitrary units
    Concentration in Tube 2 = 0.01 arbitrary units
  2. Set up division ratio:
    100 ÷ 0.01 (1 mark)
  3. Calculate the final value:
    10 000 or 10⁴ times (1 mark)

❌ Common Calculation Traps

  • Subtracting instead of dividing: 100 - 0.01 = 99.99 (gets 0 marks; "how many times more" means a ratio/division).
  • Dividing upside down: 0.01 ÷ 100 = 0.0001 .
  • Miscounting zeros when dividing by a decimal: 100 ÷ 0.01 is equivalent to 100 × 100 = 10 000 , not 1 000.
Mark allocation: [1 mark] for correct substitution 100 / 0.01 ; [1 mark] for final answer of 10 000 .
Question 07.3 • 1 Mark

Optimum Concentration for Root Growth

Identifying the most effective concentration from visual evidence

✅ Correct Answer

Tick the second box:

☑ 0.1 arbitrary units

🧠 Exam Technique

Scan Figure 8 for the stem showing the greatest root development. Tube 3 visibly possesses the longest, most extensive cluster of roots, which matches a concentration of 0.1 arbitrary units.

Mark allocation: 1 mark for correctly ticking 0.1 arbitrary units only.
Question 07.4 • 1 Mark

Interpreting Toxicity at High Concentrations

Providing observational evidence that chemical Q can be harmful

✅ Correct Answer

  • Reduced / no root growth at high(er) concentrations.
  • OR specifically stating: no roots grew in Tube 6 / at concentration 100.

❌ Common Errors

  • Stating unqualified trends like "the higher the concentration, the fewer roots grow" (this is false across the whole range because roots increase up to 0.1).
  • Saying "the plant died" without referring to the observed evidence (root growth).
Mark allocation: 1 mark for linking high concentration (or 100 units / Tube 6) to reduced or absent root formation.
Question 07.5 • 4 Marks

Cuttings vs Seeds: Asexual vs Sexual Reproduction

Explaining why taking cuttings is advantageous for preserving desirable floral traits

✅ Key Points Needed (Indicative Content)

  • Genetically Identical Offspring: Cuttings are clones produced by asexual reproduction involving mitosis.
  • Phenotype Guarantee: All new plants will inherit the same alleles/genes as Plant A, guaranteeing large, brightly-coloured flowers.
  • No Variation: Seeds are produced by sexual reproduction (involving meiosis and fertilisation/pollination), which mixes alleles and causes variation—flowers might end up small or dull.
  • Speed: Growing cuttings is quicker because you don't need to wait for seed formation, pollination, or germination.

🧠 Level 2 Marking Criteria (3–4 marks)

To reach Level 2, you must give reasons and clearly link them to biological causes:

  • Link cuttings to clones / identical alleles → ensuring desired flower appearance.
  • Link seeds to sexual reproduction / mixing of alleles → causing unpredictable variation.

💡 Biology in Context

Cuttings (Asexual Reproduction):

  • 1 parent plant
  • Cell division: Mitosis only
  • Offspring: Clones (no genetic variation)

Seeds (Sexual Reproduction):

  • Gamete fusion (pollen + ovule)
  • Cell division: Meiosis to make gametes
  • Offspring show genetic variation

❌ Common Errors

  • Confusing mitosis and meiosis (cuttings grow into plants by mitosis).
  • Saying "cuttings produce identical traits" without explaining why (they have the exact same genes/alleles).
  • Forgetting to explain why seeds are disadvantageous here (cross-pollination causes variation).
Examiner Insight: Top answers clearly contrast both methods: "Taking cuttings is asexual reproduction involving mitosis, so all offspring are genetically identical clones with the exact same alleles. This guarantees the flowers will be large and brightly coloured. Seeds result from sexual reproduction and pollination, which introduces genetic variation so the flowers may lose these traits."

Topics

Biology · B6: Inheritance, Variation and Evolution

Question and mark scheme from the AQA GCSE Biology examination, Biology Paper 2 (Foundation), June 2024. QuestionVault is an independent revision resource; questions remain the copyright of the awarding body.