AQA GCSE Biology Biology Paper 2 (Foundation), June 2024: Question 7
9 marks · Standard Demand difficulty · Extended Answer
Analyze an investigation into the effect of chemical Q on root growth in geranium cuttings, calculate concentration differences, and explain why cuttings are preferred over seeds.
Practise this questionQuestion
Question text
07 Gardeners can grow plants from:
• seeds
• cuttings taken from adult plants.
A gardener investigated the growth of roots on cuttings from a geranium plant.
This is the method used.
1. Take 6 cuttings from the stems of the same plant.
2. Prepare 6 test tubes, each containing a different concentration of a solution of
chemical Q.
3. Place 1 cutting in each test tube with the cut end of each stem in the solution.
4. Leave the test tubes at room temperature for 10 days.
Figure 8 shows the results.
Figure 8
07.1 Tube 1 contains no chemical Q.
Tube 1 is a control.
Why did the gardener include tube 1 in the investigation?
[1 mark]
*0287*2
. How many times more concentrated is chemical Q in tube 6 than in tube 2?
[2 marks]
Number of times more concentrated =
07.3 What was the best concentration of chemical Q for stimulating root growth?
[1 mark]
Tick ( ) one box.
0.01 arbitrary units
0.1 arbitrary units
1 arbitrary unit
10 arbitrary units
07.4 Give evidence from Figure 8 that a high concentration of chemical Q may be toxic to
geranium plants.
[1 mark]
07.5 The gardener has four types of geranium plant: A, B, C and D.
Plant A produces larger, more brightly-coloured flowers than any of the other plants.
*29* The gardener wants to grow more plants of type A.
Explain why the gardener chooses to take cuttings from plant A instead of growing
seeds from plant A.
[4 marks]
Mark scheme
Show the mark scheme
Question 7
AO /
Question Answers Extra information Mark
Spec Ref.
07.1 ignore as a control AO2
do not accept control variable 4.6.2.5
to compare (with the other allow to show the difference 1
tubes)
or
to show the effect of chemical Q
AO /
Spec Ref.
07.2 100 1 AO2
0.01 4.6.2.5
10 000 / 104 (times) 1
AO /
Spec Ref.
07.3 0.1 arbitrary units 1 AO3
4.6.2.5
AO /
Spec Ref.
07.4 reduced / no (root) growth at allow no (root) growth in tube 6 / 1 AO3
high(er) concentrations 100 4.6.2.5
allow answer in terms of specific
tubes / concentrations
ignore the higher the
concentration, the fewer roots– – –
grow unqualified
AO /
Question Answers Mark
Spec Ref.
07.5 Level 2: Relevant points (reasons/causes) are identified, given in 3–4 AO2
detail and logically linked to form a clear account.
Level 1: Points are identified and stated simply, but their relevance 1–2 AO3
is not clear and there is no attempt at logical thinking.
No relevant content 0
4.6.1.1
Indicative content 4.6.1.3
4.6.2.1
Taking cuttings: 4.6.2.5
• quicker
o (because) do not have to wait as long for flowers / fruits /
seeds (to form)
• all offspring identical (in appearance) or all have large /
brightly-coloured flowers
o (because) all have the same alleles / genes / DNA
• asexual reproduction or not sexual reproduction or cloning
o (so) no fusing of gametes
o (so) no mixing of alleles / genes from two parents
o (so) offspring are identical (in appearance)
• involves mitosis
o (which) copies genetic material / chromosomes
• does not involve meiosis
o (which) gives different genetic material / chromosomes
• avoids variation in flowers
o due to pollination / cross pollination (from other geraniums)
o (as) no mixing of alleles / genes from two parents
• increased profitability
o less resources needed
o quicker turnover
o consistent quality of plants / flowers
Total Question 7 9
How to answer it
Plant Hormones, Cuttings & Asexual Reproduction
What this question tests
This question assesses experimental design in biology (understanding experimental controls and interpreting concentration effects), quantitative skills (ratio comparisons with decimals), data interpretation from diagrams, and the biological differences between sexual reproduction (seeds) and asexual reproduction / cloning (stem cuttings).
Purpose of an Experimental Control
Explaining why Tube 1 (0 arbitrary units of chemical Q) was set up
✅ Correct Answer
Either of the following:
- To compare with the other tubes (to see how roots grow without the chemical).
- To show the effect of chemical Q on root growth.
🧠 Exam Technique
When asked why a "zero concentration" or untreated setup is included, examiners want the word compare or an explicit statement about identifying the effect of the independent variable.
❌ Common Errors
- Writing "as a control" (simply repeating the question gets 0 marks).
- Calling it a "control variable" (a control tube is a baseline comparison, not a variable being kept constant).
- Vague claims like "to make it a fair test".
💡 Key Knowledge
A control experiment provides a baseline measurement. It proves that any observed root growth in tubes 2–6 was caused specifically by chemical Q, rather than just water or room temperature.
Relative Concentration Calculation
Calculating how many times more concentrated Tube 6 is than Tube 2
📐 Step-by-Step Calculation
- Identify values from Figure 8:
Concentration in Tube 6 = 100 arbitrary units
Concentration in Tube 2 = 0.01 arbitrary units - Set up division ratio:
100 ÷ 0.01 (1 mark) - Calculate the final value:
10 000 or 10⁴ times (1 mark)
❌ Common Calculation Traps
- Subtracting instead of dividing: 100 - 0.01 = 99.99 (gets 0 marks; "how many times more" means a ratio/division).
- Dividing upside down: 0.01 ÷ 100 = 0.0001 .
- Miscounting zeros when dividing by a decimal: 100 ÷ 0.01 is equivalent to 100 × 100 = 10 000 , not 1 000.
Optimum Concentration for Root Growth
Identifying the most effective concentration from visual evidence
✅ Correct Answer
Tick the second box:
☑ 0.1 arbitrary units
🧠 Exam Technique
Scan Figure 8 for the stem showing the greatest root development. Tube 3 visibly possesses the longest, most extensive cluster of roots, which matches a concentration of 0.1 arbitrary units.
Interpreting Toxicity at High Concentrations
Providing observational evidence that chemical Q can be harmful
✅ Correct Answer
- Reduced / no root growth at high(er) concentrations.
- OR specifically stating: no roots grew in Tube 6 / at concentration 100.
❌ Common Errors
- Stating unqualified trends like "the higher the concentration, the fewer roots grow" (this is false across the whole range because roots increase up to 0.1).
- Saying "the plant died" without referring to the observed evidence (root growth).
Cuttings vs Seeds: Asexual vs Sexual Reproduction
Explaining why taking cuttings is advantageous for preserving desirable floral traits
✅ Key Points Needed (Indicative Content)
- Genetically Identical Offspring: Cuttings are clones produced by asexual reproduction involving mitosis.
- Phenotype Guarantee: All new plants will inherit the same alleles/genes as Plant A, guaranteeing large, brightly-coloured flowers.
- No Variation: Seeds are produced by sexual reproduction (involving meiosis and fertilisation/pollination), which mixes alleles and causes variation—flowers might end up small or dull.
- Speed: Growing cuttings is quicker because you don't need to wait for seed formation, pollination, or germination.
🧠 Level 2 Marking Criteria (3–4 marks)
To reach Level 2, you must give reasons and clearly link them to biological causes:
- Link cuttings to clones / identical alleles → ensuring desired flower appearance.
- Link seeds to sexual reproduction / mixing of alleles → causing unpredictable variation.
💡 Biology in Context
Cuttings (Asexual Reproduction):
- 1 parent plant
- Cell division: Mitosis only
- Offspring: Clones (no genetic variation)
Seeds (Sexual Reproduction):
- Gamete fusion (pollen + ovule)
- Cell division: Meiosis to make gametes
- Offspring show genetic variation
❌ Common Errors
- Confusing mitosis and meiosis (cuttings grow into plants by mitosis).
- Saying "cuttings produce identical traits" without explaining why (they have the exact same genes/alleles).
- Forgetting to explain why seeds are disadvantageous here (cross-pollination causes variation).
Topics
Biology · B6: Inheritance, Variation and Evolution
Question and mark scheme from the AQA GCSE Biology examination, Biology Paper 2 (Foundation), June 2024. QuestionVault is an independent revision resource; questions remain the copyright of the awarding body.