AQA GCSE Biology Biology Paper 1 (Foundation), June 2025: Question 1

12 marks · Low Demand difficulty · Short Answer

Identify animal and plant cell structures, state functions of specialised cells, and determine methods and directions of substance transport across cells.

Practise this question

Question

Question 1 contains parts 01.1 to 01.8 concerning cell biology. 01.1 features Figure 1, a microscopic image of an animal cell with cell membrane and nucleus labelled, asking to identify Part X as cytoplasm, mitochondrion, or vacuole. 01.2 asks for one structure found in the nucleus. 01.3 asks for one plant cell part not found in animal cells. 01.4 asks which substance root hair cells absorb from soil: glucose, protein, or water. 01.5 asks for the function of a sperm cell. 01.6 requires drawing lines from Nerve cell and Xylem cell to organism types: Animals, Bacteria, Fungi, Plants. 01.7 asks to select two processes moving substances into/out of cells from active transport, diffusion, mitosis, mutation, and respiration. 01.8 asks to tick the direction of movement for carbon dioxide, oxygen, and water between blood and muscle cells in Table 1.
Question text

01 This question is about cells.

01.1 Figure 1 shows an animal cell seen through a microscope.

Figure 1

What is part X in Figure 1?

[1 mark]

Tick ( ) one box.

Cytoplasm

Mitochondrion

Vacuole

01.2 Name one structure found in the nucleus.

[1 mark]

01.3 Name one cell part that is found in a plant cell but is not found in an animal cell.

[1 mark]

01.4 Root hair cells are found in plants.

Which substance do root hair cells absorb from the soil?

[1 mark]

Tick ( ) one box.

Glucose

Protein

Water

01.5 A sperm cell is a specialised cell.

What is the function of a sperm cell?

[1 mark]

01.6 Draw one line from each specialised cell to the type of organism where the

specialised cell is found.

[2 marks]

01.7 The cell membrane controls which substances move into and out of cells.

Which two processes move substances into and out of cells?

[2 marks]

Tick ( ) two boxes.

Active transport

Diffusion

Mitosis

Mutation

Respiration 5

01.8 Table 1 shows substances that are exchanged between the blood and muscle cells.

Complete Table 1 to show the direction of movement of each substance.

[3 marks]

Tick ( ) one box in each row.

Table 1

Direction of movement of substance

*04* From blood to

Substance

ONLY from blood ONLY from muscle muscle cells AND

to muscle cells cells to blood from muscle cells

to blood

Carbon dioxide

Oxygen

Water

Mark scheme

Show the mark scheme Mark scheme for Question 1: 01.1 cytoplasm (1 mark). 01.2 chromosome(s) or gene(s), allowing DNA, allele, or genetic material (1 mark). 01.3 cell wall, permanent vacuole, or chloroplast (1 mark). 01.4 water (1 mark). 01.5 fertilisation, swimming to egg, or providing chromosomes/DNA (1 mark). 01.6 Nerve cell linked to Animals, Xylem cell linked to Plants (2 marks). 01.7 active transport and diffusion (2 marks). 01.8 Carbon dioxide moving only from muscle cells to blood, Oxygen moving only from blood to muscle cells, and Water moving from blood to muscle cells and vice versa (3 marks). Total marks: 12.

Question 1

AO /

Question Answers Extra information Mark

Spec Ref.

01.1 cytoplasm 1 AO1

4.1.1.2

AO /

Spec Ref.

01.2 any one from: 1 AO1

• chromosome(s) 4.1.1.1

• gene(s) 4.1.1.2

allow DNA / allele 4.1.2.1

allow genetic material

ignore genetic information

AO /

Spec Ref.

01.3 any one from: 1 AO1

• (cell) wall ignore cellulose 4.1.1.2

• (permanent / large) vacuole

• chloroplast ignore chlorophyll

AO /

Spec Ref.

01.4 water 1 AO1

4.1.3.3

4.2.3.2

AO /

Spec Ref.

01.5 any one from: 1 AO1

4.1.1.3

• fertilisation allow to fuse / join with an egg

allow to penetrate the egg

• to swim to the egg allow to reach the egg

ignore swim unqualified

• to provide chromosomes / allow to provide genetic material

DNA for the egg / information for the egg

• to carry chromosomes / DNA

from the male

AO /

Question Answers Mark

Spec Ref.

01.6 AO1

4.1.1.3

4.2.3.1

4.2.3.2 7

do not accept more than one line from a box on the left

AO /

Spec Ref.

01.7 active transport 1 AO1

4.1.3.1

diffusion 1 4.1.3.3

4.1.1.2

AO /

Spec Ref.

01.8 AO2

Direction of movement of substance 4.1.3.1

From blood 4.1.3.2

ONLY from ONLY from to muscle 4.4.2.1

Substance

blood to muscle cells cells AND

muscle cells to blood from muscle

cells to blood

Carbon 1

dioxide

Oxygen 1

Water 1

do not accept more than one tick in any row

Total Question 1 12

How to answer it

Cell Biology: Structure, Specialisation & Transport

📋 What this question tests

This Foundation-tier question assesses your core knowledge across cell biology (Specification 4.1):

  • Identifying basic eukaryotic cell parts (nucleus, cytoplasm, cell membrane).
  • Distinguishing between animal and plant sub-cellular structures.
  • Recalling functions of specialised cells (sperm, nerve, xylem, root hair).
  • Identifying transport processes across cell membranes (active transport, diffusion).
  • Applying knowledge of respiration and osmosis to exchange surfaces (muscle and blood).

Question 01.1: Animal Cell Organelles

Identifying Part X in a light microscope image

✅ Correct Answer

Tick: Cytoplasm

[1 mark] · AO1 (Spec 4.1.1.2)

💡 Key Knowledge

Under a school light microscope, animal cells typically reveal three main parts:

  • Nucleus: Dark central spot containing DNA.
  • Cell membrane: The outer boundary.
  • Cytoplasm: The jelly-like fluid filling the space between.

❌ Common Errors

Selecting vacuole: Animal cells do not have permanent vacuoles (only plants do). Mitochondria are far too small to see clearly without an electron microscope.

Question 01.2: Nuclear Structures

Naming a structure found inside the nucleus

✅ Correct Answer (Any one)

  • Chromosome(s)
  • Gene(s)
  • Also accepted: DNA, allele, genetic material
[1 mark] · AO1 (Spec 4.1.1.1, 4.1.2.1)

🧠 Exam Technique: Be Precise

The mark scheme explicitly says "ignore genetic information".

"Information" is an abstract concept, not a physical biological structure or chemical molecule. Always name the actual physical structure: chromosome or DNA.

Question 01.3: Plant vs Animal Cells

Structures present in plant cells but NOT animal cells

✅ Correct Answer (Any one)

  • (Cell) wall
  • (Permanent / large) vacuole
  • Chloroplast
[1 mark] · AO1 (Spec 4.1.1.2)

❌ Common Trap: Structure vs Substance

  • Chloroplast vs Chlorophyll: The chloroplast is the organelle (structure); chlorophyll is the chemical pigment inside it. The mark scheme explicitly states: ignore chlorophyll.
  • Cell wall vs Cellulose: Cell wall is the structure; cellulose is what it is made of. The mark scheme explicitly states: ignore cellulose.

Question 01.4: Root Hair Cells

Identifying absorbed substances from soil

✅ Correct Answer

Tick: Water

[1 mark] · AO1 (Spec 4.1.3.3, 4.2.3.2)

💡 Key Knowledge

Root hair cells absorb two main substances from the soil:

  • Water by osmosis.
  • Mineral ions (such as nitrates and magnesium) by active transport.

Plants make their own glucose via photosynthesis—they never absorb glucose or protein from the soil!

Question 01.5: Function of a Sperm Cell

Specialised cell adaptations & function

✅ Correct Answer (Any one)

  • Fertilisation / to fertilise an egg
  • To swim to the egg (or join / fuse / reach the egg)
  • To carry / provide male genetic material (DNA / chromosomes) to the egg
[1 mark] · AO1 (Spec 4.1.1.3)

🧠 Exam Technique

Saying just "to swim" gets 0 marks (the mark scheme notes "ignore swim unqualified"). You must state the purpose of the movement: to reach or fertilise the egg.

Question 01.6: Specialised Cells & Organisms

Matching specialized cells to their organism type

✅ Correct Answer

  • Nerve cell ➔ Animals [1 mark]
  • Xylem cell ➔ Plants [1 mark]
[2 marks] · AO1 (Spec 4.1.1.3, 4.2.3.1)

💡 Specialised Cell Roles

  • Nerve cell: Long axon to carry electrical impulses throughout an animal's body.
  • Xylem cell: Hollow, lignified dead tubes that carry water and mineral ions up a plant stem.

❌ Line Drawing Rule

The command states "Draw one line from each...". If you draw two lines from "Nerve cell" or "Xylem cell", you forfeit the mark for that box completely.

Question 01.7: Membrane Transport

Identifying transport mechanisms

✅ Correct Answer (Tick two)

  • ☑ Active transport [1 mark]
  • ☑ Diffusion [1 mark]
[2 marks] · AO1 (Spec 4.1.3.1, 4.1.3.3)

💡 Key Processes to Remember

  • Diffusion: Passive movement of particles from high to low concentration.
  • Active Transport: Movement against a concentration gradient using energy from respiration.
  • Osmosis: Diffusion of water molecules across a partially permeable membrane.

❌ Distractors Explained

  • Mitosis = cell division (making identical body cells).
  • Mutation = change in DNA base sequence.
  • Respiration = chemical reaction inside cells releasing energy.

Question 01.8: Exchange Between Blood and Muscle Cells

Completing the direction of substance exchange table

Substance Direction of movement of substance
ONLY from blood to muscle cells ONLY from muscle cells to blood From blood to muscle cells AND from muscle cells to blood
Carbon dioxide ✓ [1 mark]
Oxygen ✓ [1 mark]
Water ✓ [1 mark]

💡 Biological Reasoning

  • Oxygen: High concentration in blood capillaries, continually consumed inside muscle cells for aerobic respiration. Net movement is only blood ➔ muscle.
  • Carbon dioxide: Waste product produced by aerobic respiration in muscle cells. High in muscle, low in blood. Net movement is only muscle ➔ blood.
  • Water: Moves in both directions across cell membranes by osmosis depending on hydration and solute levels.

❌ Common Error on Water

Many students wrongly tick "ONLY from muscle cells to blood" because water is a product of respiration. However, water constantly moves dynamically in both directions across the cell membrane to maintain osmotic balance.

[3 marks total] · AO2 (Spec 4.1.3.1, 4.1.3.2, 4.4.2.1)

Topics

Biology · B1: Cell Biology · B2: Organisation

Question and mark scheme from the AQA GCSE Biology examination, Biology Paper 1 (Foundation), June 2025. QuestionVault is an independent revision resource; questions remain the copyright of the awarding body.