AQA GCSE Combined Science: Trilogy Biology Paper 2 (Higher), 2018: Question 1
15 marks · Standard Demand difficulty · Extended Answer
Investigate how light intensity affects the distribution and percentage cover of small plants growing under and around a tree, including classifying factors, identifying equipment, calculating sampled area, and interpreting results.
Practise this questionQuestion
Question text
01 Many biotic and abiotic factors can affect the growth of plants.
01.1 Are the factors in Table 1 biotic or abiotic?
[2 marks]
Tick one box for each factor.
Table 1
Factor Biotic Abiotic
Diseases
Herbivores
Temperature
Water
Two students investigated the effect of light intensity on the distribution of
small plants.
The plants are growing under a tree in a park.
The students made the following hypothesis:
‘As you move outwards from a tree there will be more plant growth.’
01.2 Explain why the students thought their hypothesis would be correct.
[3 marks]
01.3 The students used two pieces of equipment.
Give the scientific name of each piece of equipment.
[2 marks]
A square frame measuring 0.5 m × 0.5 m
An electronic device to measure light intensity
This is the method used.
1. Fix one end of a tape measure at the base of the tree.
2. Fix the other end of the tape measure 11 metres from the tree.
3. At 0 metres put the square frame on the ground.
4. Identify all the plant species growing inside the frame.
5. Estimate and record the percentage cover of each plant species.
6. Measure the light intensity inside the frame.
7. Put the square frame on the ground every 2 metres along the tape to 10 metres.
8. Repeat steps 4 – 6 in every frame.
Figure 1 shows the equipment in this investigation.
Figure 1
01.4 Calculate the total area sampled.
[1 mark]
Total area sampled =4 m2
01.5 The whole investigation was done as quickly as possible on the same day.
Suggest one reason why.
[1 mark]
01.6 Give one way the investigation could be improved.
[1 mark]
Table 2 shows the results.
Table 2
Distance from tree in metres
02 4 6 8 10
Percentage cover of grass 15 50 35 16 15 15
Percentage cover of plantain 0 5 10 40 25 30
Percentage cover of daisy 0 0 0 4 20 10
Percentage cover of clover 1 10 25 40 40 45
Total percentage cover of plants 16 65 70 100 100 100
Light intensity in arbitrary units 37 59 150 175 >200 >200
01.7 Which plant species in Table 2 will only grow at high light intensity?
[1 mark]
01.8 What conclusion can be made about the relationship between light intensity and the
total percentage cover of plants?
Use data from Table 2 in your answer.
[2 marks]
01.9 Light intensity might not be the cause of this pattern of plant distribution.
Suggest one different factor that may cause these results.
Give one reason for your answer.
[2 marks]
Factor
Reason
Mark scheme
Show the mark scheme
AO /
Question Answers Extra information Mark
Spec. Ref.
01.1 Factor Biotic Abiotic allow 1 mark for 2 or 3 correct 2 AO1
4.7.1.3
Diseases 4.7.1.2
Herbivores
Temp
Water
01.2 (leaves block light near tree so) allow low light intensity under 1 AO2
more light (as you move tree 4.7.2.1
outwards) ignore Sun
for photosynthesis allow less photosynthesis under 1 AO1
the tree 4.7.2.1
4.4.1.1
4.4.1.3
(which) produces (more) ignore growth 1 AO1
glucose / proteins (for growth) ignore food 4.7.2.1
4.4.1.1
allow molecules, cell 4.4.1.3
components or other correct
substances instead of proteins
if no other mark awarded allow
less water / ions / minerals /
nutrients under the tree
01.3 in this order AO1
4.7.2.1
quadrat correct spelling only 1
light meter allow lux meter 1
allow light intensity meter
allow light data logger
01.4 1.5(0) (m2 ) allow 15 000 cm2 1 AO2
4.7.2.1
AO /
Spec. Ref.
01.5 to keep light (intensity) as allow the light (intensity) might 1 AO3
similar as possible change 4.7.2.1
ignore references to
temperature 7
ignore weather
ignore Sun
01.6 any one from: 1 AO3
• repeat (investigation) around allow repeat in different 4.7.2.1
the tree directions
• repeat (investigation) for
other trees / areas
• sample every one metre
• count the number of each
species present (rather than
percentage cover)
ignore repeats unqualified
ignore repeat at different times /
days / seasons
ignore different size quadrat
ignore random sampling
01.7 daisy 1 AO3
4.7.2.1
01.8 as light (intensity) increased so ignore directly proportional 1 AO3
did the percentage / cover of ignore positive correlation 4.7.2.1
plants unqualified
up to 100% / maximum at 175 1
(arbitrary units)
ignore distance
AO /
Spec. Ref.
01.9 ignore carbon dioxide 2 AO2
do not accept oxygen 4.7.2.1
any pair from:
• (lack of) water / rain (1) allow soil moisture
because the leaves are
stopping the rain
or
because the roots of the
tree are absorbing it (1) 8
• (lack of) minerals / ions (1) allow magnesium / nitrate /
nutrients
because the tree (roots)
have absorbed them (1)
• temperature (1) allow too cold / cooler
because less thermal energy allow ‘heat’ for thermal energy
from the sun is reaching
under the tree canopy (1)
allow pH / acidity (1)
because (some) fallen leaves
are acidic (1)
Total 15
How to answer it
Plant Distribution Under a Tree
You need to know the difference between biotic and abiotic factors, explain how light intensity affects plant growth, identify fieldwork equipment, do a simple area calculation, and interpret a results table using scientific evidence.
Question overview
GCSE AQA Combined Science: Trilogy – Ecology fieldwork and plant distribution
Key topic
- Biotic vs abiotic factors
- Light intensity and photosynthesis
- Sampling with a quadrat
- Reading tables and spotting patterns
How marks are gained
- Recall correct science facts
- Use data from the table
- Give reasons, not just statements
- Show calculations clearly
Part (a) – Biotic or abiotic factors
Q1.1: Identify the type of each factor
✅ Correct answers
- Diseases → biotic
- Herbivores → biotic
- Temperature → abiotic
- Water → abiotic
💡 Key knowledge
- Biotic = living factors, or things caused by living organisms.
- Abiotic = non-living environmental factors.
- Diseases and herbivores involve living organisms, so they are biotic.
- Temperature and water are part of the environment, so they are abiotic.
🧠 Exam technique
- There are 2 marks, so you can get credit for any 2 or 3 correct labels.
- Tick carefully in the correct column.
- If you are unsure, think: living = biotic, non-living = abiotic.
❌ Common errors
- Calling water biotic because plants need it.
- Calling temperature biotic because it affects living things.
- Mixing up the meaning of “biotic” and “abiotic”.
Part (b) – Why the hypothesis makes sense
Q1.2: Explain why moving away from the tree could increase plant growth
✅ Full-mark answer
Leaves block light near the tree, so there is more light as you move outwards. More light means more photosynthesis. Photosynthesis makes more glucose, which plants use for growth.
💡 Key knowledge
- Under the tree canopy, less light reaches the plants.
- Light is needed for photosynthesis.
- More photosynthesis means more glucose is produced.
- Glucose is used to make biomass for growth, including proteins and other cell materials.
🧠 Exam technique
This is a 3-mark explain question. A strong answer links the ideas in a chain:
less light → more photosynthesis → more glucose → more growth
To get the marks, explain the cause and the effect, not just one word like “light”.
❌ Common errors
- Just writing “plants grow better in sunlight” without explaining why.
- Talking about the Sun instead of the light level under the tree.
- Saying “photosynthesis makes growth” without mentioning glucose or biomass.
Part (c) – Fieldwork equipment
Q1.3: Name the scientific equipment
✅ Correct answers
- A 0.5 m × 0.5 m square frame = quadrat
- An electronic device to measure light intensity = light meter
💡 Key knowledge
- A quadrat is used to sample plants in a fixed area.
- A light meter measures light intensity in a habitat.
- “Lux meter” or “light intensity meter” may also be accepted.
🧠 Exam technique
- Check the object’s purpose, then name it.
- Spelling matters for 1-mark recall answers.
- Answers must be in the correct order given on the paper.
❌ Common errors
- Writing “square” instead of quadrat.
- Writing “sensor” or “meter” without the right scientific name.
- Confusing the quadrat with the tape measure.
Part (d) – Area sampled
Q1.4: Calculate the total area sampled
📐 Calculation
Step 1: Find the area of one quadrat
0.5 m × 0.5 m = 0.25 m²
Step 2: Count how many quadrats were used
They were placed every 2 m from 2 m to 10 m:
2, 4, 6, 8, 10 → 5 quadrats
Step 3: Multiply
0.25 × 5 = 1.25 m²
Answer: 1.25 m²
🧠 Exam technique
- Write the unit clearly: m².
- Method marks are based on the correct area of one quadrat and the correct number of samples.
- Even if your arithmetic is weak, show the working for credit.
❌ Common errors
- Using 0.5 + 0.5 instead of multiplying.
- Forgetting that area is in m², not m.
- Counting the wrong number of quadrats.
- Giving 12500 cm² without showing the conversion clearly.
Part (e) – Why the investigation was done quickly
Q1.5: Suggest one reason
✅ Correct answer
To keep the light intensity as similar as possible.
💡 Key knowledge
- Environmental conditions can change during an investigation.
- Light intensity can change as the time of day changes or clouds move.
- Doing the sampling quickly helps make the results fairer.
🧠 Exam technique
The mark scheme focuses on control of light intensity. A short, clear reason is enough for 1 mark.
❌ Common errors
- Talking only about temperature or weather, which the mark scheme does not require here.
- Saying “to save time” without linking it to fair testing.
Part (f) – How to improve the investigation
Q1.6: Give one way the investigation could be improved
✅ Acceptable improvements
- Repeat the investigation around the tree.
- Repeat the investigation for other trees or areas.
- Sample every one metre.
- Count the number of each species present rather than percentage cover.
💡 Key knowledge
- Repeating gives more reliable results.
- Sampling more often gives a better picture of distribution.
- Using multiple sites helps check whether the pattern is general.
🧠 Exam technique
- Give a practical improvement, not just “do more science”.
- Strong answers often mention repeat or more frequent sampling.
❌ Common errors
- “Use a bigger quadrat” — not accepted here.
- “Random sampling” — not accepted for this mark scheme.
- Repeats without explaining what is repeated.
Part (g) – Species at high light intensity
Q1.7: Which plant species will only grow at high light intensity?
✅ Correct answer
Daisy
💡 Key knowledge
- Daisy cover is 0 at low light levels and appears more at higher light.
- This suggests it needs more light to grow well.
🧠 Exam technique
Use the table to pick the species that only appears where light is high. One word is enough for the mark.
Part (h) – Relationship between light intensity and plant cover
Q1.8: What conclusion can be made from Table 2?
✅ Full-mark conclusion
As light intensity increased, the total percentage cover of plants increased, up to 100% at 175 arbitrary units.
💡 Key knowledge
- From the table, total plant cover rises from 16% at 37 units to 100% at 175 units.
- At 200+ units, plant cover stays at 100%.
- This shows a positive correlation.
🧠 Exam technique
- Always support the conclusion with data.
- Use comparative words: as... increased..., from... to...
- Do not say “directly proportional” unless the pattern is perfectly proportional.
❌ Common errors
- Saying “plants increase because of distance” without mentioning light intensity.
- Using only one value with no trend.
- Ignoring the plateau at 100%.
Part (i) – Another factor that could affect the pattern
Q1.9: Suggest a different factor and explain why
✅ Acceptable answers
- Water / rain / soil moisture – because the tree canopy or roots affect how much water reaches the area.
- Minerals / ions – because the tree roots absorb nutrients from the soil.
- Temperature – because it is cooler under the tree canopy.
- pH / acidity – because fallen leaves can make the soil more acidic.
💡 Key knowledge
- Plants need water and mineral ions for growth.
- Tree roots compete with other plants for resources.
- The canopy can reduce sunlight and also change local conditions like temperature and moisture.
🧠 Exam technique
This is a 2-mark answer: you need
- one factor, and
- one clear reason linked to the tree or habitat.
A good structure is: Factor: water then Reason: less rain reaches under the tree because the leaves block it .
❌ Common errors
- Choosing carbon dioxide — not accepted here.
- Choosing oxygen — not accepted.
- Giving a factor with no explanation.
- Writing an unrelated factor like “pollution” without linking it to the data.
Quick revision summary
Biotic vs abiotic
- Biotic = living things or their effects
- Abiotic = non-living factors
Light and growth
- More light → more photosynthesis
- More photosynthesis → more glucose
- More glucose → more growth
Fieldwork
- Quadrat = sampling frame
- Light meter = measures light intensity
- Repeat samples for reliability
Topics
Biology · Required Practicals · B7: Ecology · B4: Bioenergetics · Biology Required Practicals
Question and mark scheme from the AQA GCSE Combined Science: Trilogy examination, Biology Paper 2 (Higher), 2018. QuestionVault is an independent revision resource; questions remain the copyright of the awarding body.