AQA GCSE Combined Science: Trilogy Biology Paper 1 (Higher), 2019: Question 3
13 marks · Standard Demand difficulty · Extended Answer
Interpret and use data from a potometer investigation to explain transpiration, calculate rate of water uptake, plot results and predict the effect of different environmental conditions.
Practise this questionQuestion
Question text
03 A student used a potometer to investigate the rate of water uptake in a plant shoot.
Figure 3 shows a potometer.
Figure 3
As the shoot takes in water the air bubble moves.
The rate of water uptake is the distance the air bubble moves in a given time.
This is the method used.
1. Place the potometer in moist air at 25 °C
2. Position the air bubble at 0 mm in the capillary tube.
3. Record the position of the air bubble in the capillary tube every minute
for 5 minutes. 11
4. Repeat steps 2 and 3 with the potometer in different conditions.
Table 3 shows the conditions used.
Table 3
Investigation Conditions
A Moist air at 25 °C
B Dry air at 15 °C
C Dry air at 25 °C
03.1 After investigation A the air bubble had moved part way along the capillary tube.
*10* Suggest how the student moved the air bubble back to 0 mm for the start of
investigation B.
[1 mark]
03.2 Capillary tubing is very narrow.
Explain why narrow tubing was used.
[2 marks]
Figure 4 shows the results for investigation A.
Figure 4
The cross-sectional area of the capillary tube was 0.8 mm2
03.3
Calculate the rate of water uptake for investigation A in mm3/min
[3 marks]
13 Rate = mm3/min
03.4 Table 4 shows the results from investigation B.
Table 4
Time in minutes Position of air bubble in mm
*12* 0 0
2 16
3 22
4 30
5 42
Plot the data from Table 4 on Figure 4.
You should:
• draw a line of best fit
• label the line B.
[3 marks]
03.5 Investigation C was carried out in dry air at 25 °C
Draw a line on Figure 4 to show the results you would expect for investigation C.
Label the line C.
[1 mark]
03.6 The investigations were carried out in daylight.
The air bubble would not move if the investigations were done in the dark.
Explain why.
[3 marks]
Mark scheme
Show the mark scheme
AO /
Question Answers Extra information Mark
Spec. Ref.
AO3
03.1 opened and closed the tap (so allow opened the tap (carefully / 1 4.2.3.2
water enters from reservoir) gently)
03.2 air bubble moves further (in a allow air bubble moves faster 1 AO3
given time) 4.2.3.2
(so) resolution is improved allow it is easier to see a small 1
change (in volume)
ignore is easier to measure
unqualified
allow measurements are more
accurate
ignore to make test more
accurate
ignore references to precision or
validity
an answer of 10.56 (mm3/min) AO2
03.3 scores 3 marks 4.2.3.2
66 allow tolerance of ± ½ square 1
5 allow full marks from calculation
from other correct pairs of
or readings
13.2 (mm/min) allow value in range 13 to 13.4
for only
ignore or 12.6
13.2 × 0.8 allow their calculated value in the 1
range from 12 to 14 x 0.8
10.56 (mm3/min) allow 10.6 or 11 1
AO2
03.4 points plotted correctly allow +/- ½ a square 2 4.2.3.2
allow 1 mark for 4/5 correct plots
10 suitable line of best fit 1
AO3
03.5 straight line starting at 0,0 with a 1 4.2.3.2
steeper gradient than A
03.6 no photosynthesis allow plants need light for 1 AO1
photosynthesis
(so) stomata closed (as no 1 AO2
carbon dioxide needed)
(so) no transpiration allow very little transpiration or 1 AO2
little water lost 4.2.3.2
4.4.1.2
Total 13
How to answer it
Potometer and Water Uptake
Using a potometer to measure transpiration indirectly, understanding why the apparatus is set up the way it is, reading and using graph data, calculating rate, plotting points accurately, and explaining how light affects transpiration.
Question overview
Investigating the rate of water uptake in a plant shoot using a potometer
This question is mainly about practical biology and data skills. You need to: explain a simple method, understand why certain features of the apparatus are needed, calculate a rate from a graph, and compare different environmental conditions.
Part (a) 03.1 — Resetting the bubble for investigation B
✅ Correct answer
Open and close the tap so that water from the reservoir moves the air bubble back to 0 mm.
💡 Key knowledge
- The reservoir contains water that can be used to push the bubble back.
- This lets each investigation start from the same position.
🧠 Exam technique
For 1 mark, give the action clearly. Words like open the tap and water enters from the reservoir match the mark scheme.
❌ Common errors
- Only saying “reset it” without explaining how.
- Talking about changing the bubble with air instead of using the reservoir.
Part (b) 03.2 — Why narrow capillary tubing was used
✅ Correct answer
The air bubble moves further in a given time, so the resolution is improved and small changes are easier to measure.
💡 Key knowledge
- Narrow tubing makes movement of the bubble easier to see.
- A small change in water uptake gives a bigger movement on the scale.
- This makes measurements more accurate.
🧠 Exam technique
The mark scheme rewards a cause and effect answer: narrow tube → bubble moves more → easier to measure / better resolution.
❌ Common errors
- Saying “it is more precise” without explaining why.
- Using vague phrases like “better” with no science point.
- Talking about validity, which the mark scheme does not credit here.
Part (c) 03.3 — Calculating the rate of water uptake in investigation A
📐 Calculations: step-by-step
- Read the graph carefully: at 5 minutes, the air bubble has moved to about 66 mm.
- Find the rate in mm/min: rate = distance ÷ time = 66 ÷ 5
- 66 ÷ 5 = 13.2 mm/min
- Convert to mm³/min using the cross-sectional area of the tube: 13.2 × 0.8 = 10.56 mm³/min
Final answer: 10.56 mm³/min
Accept 10.6 or 11.
✅ Correct answer
Rate = 10.56 mm³/min
💡 Key knowledge
- Rate = distance ÷ time.
- Use the graph reading correctly before calculating.
- Because the answer is in volume per minute, multiply by the tube area.
🧠 Exam technique
- Show working clearly to get method marks.
- Include units at each stage: mm/min, then mm³/min.
- If your graph reading is slightly off, you can still gain marks for the correct method.
❌ Common calculation traps
- Using 63 ÷ 5 instead of 66 ÷ 5.
- Forgetting to multiply by 0.8.
- Leaving the answer in mm/min instead of mm³/min.
- Writing the wrong units or no units at all.
Part (d) 03.4 — Plotting the results for investigation B
✅ Correct answer
Plot these points accurately and draw a suitable line of best fit:
- (0, 0)
- (1, 6)
- (2, 16)
- (3, 22)
- (4, 30)
- (5, 42)
🧠 Exam technique
- Points must be plotted correctly, usually within about half a square.
- Then draw a sensible line of best fit — not join-the-dots.
- Label the line B.
💡 Key knowledge
A line of best fit should show the overall trend and pass close to the points. It should be smooth and suitable for the data.
❌ Common errors
- Joining each point with straight line segments.
- Missing the origin point (0,0).
- Labeling the wrong line or forgetting the label.
Part (e) 03.5 — Predicting the line for investigation C
✅ Correct answer
Draw a straight line starting at 0,0 with a steeper gradient than A, and label it C.
💡 Key knowledge
Dry air at 25 °C causes faster transpiration than moist air, so the air bubble should move more quickly.
🧠 Exam technique
You do not need exact points here — just the correct shape, start, and relative steepness.
❌ Common errors
- Starting the line above 0,0.
- Drawing a curve instead of a straight line.
- Making line C less steep than A.
Part (f) 03.6 — Why the bubble would not move in the dark
✅ Correct answer
- No photosynthesis happens in the dark.
- So the stomata close because carbon dioxide is not needed.
- So there is no transpiration, or very little water loss.
💡 Key knowledge
In daylight, stomata are open for gas exchange. In the dark, photosynthesis stops, so the stomata close and water vapour loss from the leaves is reduced.
🧠 Exam technique
This is a 3-mark chain of reasoning. Try to link the ideas in order: dark → no photosynthesis → stomata close → little/no transpiration → bubble does not move.
❌ Common errors
- Saying only “plants need light” without linking it to transpiration.
- Confusing transpiration with photosynthesis.
- Forgetting the stomata connection.
Overall examiner advice
💡 What examiners wanted
- Accurate practical knowledge of a potometer.
- Clear use of units and correct graph reading.
- Reasoned explanations, especially for light/dark and transpiration.
🧠 How to get full marks
- Answer exactly what is asked: action, reason, calculation, or graph feature.
- Use scientific vocabulary: transpiration, stomata, resolution.
- Show working in calculations and always include units.
❌ Biggest traps
- Not reading the graph carefully.
- Giving vague statements with no biology link.
- Forgetting that the potometer measures water uptake indirectly, not transpiration directly.
Topics
Biology · B2: Organisation · B4: Bioenergetics
Question and mark scheme from the AQA GCSE Combined Science: Trilogy examination, Biology Paper 1 (Higher), 2019. QuestionVault is an independent revision resource; questions remain the copyright of the awarding body.