AQA GCSE Combined Science: Trilogy Biology Paper 2 (Higher), 2019: Question 1

13 marks · Standard Demand difficulty · Short Answer

Investigate the effect of drinking different amounts of caffeine on reaction time using a ruler-drop test, including method, data interpretation, control variables and why the response is not a reflex action.

Practise this question

Question

The question page describes an investigation into the effect of drinking caffeine on reaction time. Students are split into four groups, measure initial reaction time with a ruler-drop test, then drink either 200 cm^3 water, 200 cm^3 caffeine drink, 400 cm^3 caffeine drink, or 600 cm^3 caffeine drink, and repeat the test after 15 minutes. The page includes a table of caffeine mass for groups A to D with one missing value to calculate, a conversion table from ruler-drop distance in centimetres to reaction time in seconds, a scatter graph showing decrease in reaction time for groups A to D, and short questions about controls, estimating from the table, interpreting the graph, range, variables to control, and explaining why the test is not a reflex action.
Question text

01 Some students investigated the effect of drinking caffeine on reaction time.

They used a drink containing 32.25 mg of caffeine per 100 cm3

This is the method used.

1. Divide the students into four groups, A, B, C and D.

2. Measure and record the reaction time of each student using the ruler-drop test.

3. Students in:

• group A drink 200 cm3 of water

• group B drink 200 cm3 of the caffeine drink

• group C drink 400 cm3 of the caffeine drink

• group D drink 600 cm3 of the caffeine drink.

4. Repeat step 2 after 15 minutes.

01.1 Describe how to do the ruler-drop test.

[3 marks]

01.2 Table 1 shows the mass of caffeine taken in by each student.

Table 1

Group Mass of caffeine in mg

A 0

B 64.5

C 129.0

D X

Calculate value X.

*03* [1 mark]

X = mg

01.3 Why did group A drink water instead of the caffeine drink?

[1 mark]

Table 2 was used to convert the results of the ruler-drop test into reaction times.

Table 2

Distance in cm Reaction time in s Distance in cm Reaction time in s

2 0.064 28 0.239

4 0.090 30 0.247

6 0.111 32 0.256

8 0.128 34 0.263

10 0.143 36 0.271

12 0.156 38 0.278

14 0.169 40 0.286

16 0.181 42 0.293

18 0.192 44 0.300

*04* 20 0.202 46 0.306

22 0.212 48 0.313

24 0.221 50 0.319

26 0.230 52 0.326

01.4 Estimate the reaction time for a student who recorded a distance of 23 cm

[1 mark]

Reaction time = s

Students calculated the decrease in their reaction time after the drink compared with

before the drink.

Figure 1 shows the results for each student.

Figure 1

01.5 Describe the effect of the mass of caffeine taken in on the decrease in reaction time.

[1 mark]

01.6 For three students the decrease in reaction time was negative.

Give the reason why the value was negative.

[1 mark]

01.7 What is the range of results for group C?

[1 mark]

01.8 Suggest two variables that should have been controlled in this investigation.

[2 marks]

01.9 Explain why the ruler-drop test does not involve a reflex action.

[2 marks]

Mark scheme

Show the mark scheme The mark scheme is a table listing answers and extra guidance for questions 01.1 to 01.9. It gives key points for the ruler-drop method, the calculated caffeine value 193.5 mg, the estimate of 0.217 s from the conversion table, the trend that greater caffeine mass gives a greater decrease in reaction time, the reason negative decrease means reaction time was greater after the drink, the range for group C as about 0.17 s, acceptable control variables such as age, sex, sleep and using the same hand, and that the response is not automatic because it involves the conscious part of the brain. The total mark shown is 13.

AO /

Question Answers Extra information Mark

Spec. Ref.

01.1 hold a ruler (just) above the ignore near the hand 1 AO1

(open) hand of the other student 4.5.2

drop the ruler and other do not accept give verbal signal 1

student catches it

record where the ruler is caught 1

ignore timing

01.2 193.5 1 AO2

4.5.2

01.3 to compare the effect of no allow as a control (group) 1 AO3

caffeine allow to show the effect of 4.5.2

caffeine

do not accept control variable

01.4 0.217 (s) allow any value in the range 1 AO2

0.2150 to 0.2180 4.5.2

01.5 as mass of caffeine increases allow converse 1 AO3

the decrease / change in 4.5.2

reaction time increases

ignore caffeine decreases

reaction time

do not accept the greater the

increase in reaction time the

greater the mass of caffeine

01.6 their reaction time was greater allow converse 1 AO3

(after the drink) 4.5.2

allow slower / longer for greater

do not accept anomalous result

AO /

Spec. Ref.

01.7 0.01(0) to 0.18(0) 1 AO2

allow values in range 4.5.2

or 0.008 to 0.012

and

0.18(0) to 0.01(0) 0.178 to 0.182

or

0.17(0) 7

allow correct calculation from

values in range

if no values are given, allow

answers in the range 0.166 to

0.174

allow 0.01 ≤ C ≤ 0.18

ignore units

01.8 any two from: 2 AO3

• (same range of) age 4.5.2

• (same) sex / gender

• (same) height / weight / BMI

• all had no

caffeine / medication / drugs

earlier that day

• equally tired or (same)

amount of sleep

• practice of the ruler drop test

• starting point of ruler / hand allow height ruler dropped from

• same point to take

measurement above / below

the thumb / finger

• using the same hand

• (same) number of students in

each group

do not accept volume /

concentration of caffeine

01.9 not automatic allow it is a voluntary action 1 AO2

4.5.2

(because) it involves the allow because it involves 1

(conscious part of the) brain thinking / decision or conscious

action

Total 13

How to answer it

Caffeine and Reaction Time

What this question tests

You need to describe a simple practical method, use a table to calculate caffeine mass, read values from a conversion table, interpret a graph of results, explain the pattern, identify control variables, and give a scientific reason why the ruler-drop test is not a reflex.

Part 01.1

Describe how to do the ruler-drop test

✅ Correct answers

  • Hold a ruler just above the open hand of the other student.
  • Drop the ruler without warning.
  • The other student catches it.
  • Record where the ruler is caught.
Mark points are awarded for each clear step in the method.

💡 Key knowledge

  • The test measures reaction time.
  • A shorter catching distance means a faster reaction.
  • The same method should be repeated to improve reliability.

🧠 Exam technique

  • Write the steps in order.
  • Use simple practical language: hold → drop → catch → measure .
  • For full marks, describe what the person does, not just “do the test”.

❌ Common errors

  • Giving a verbal signal — this was not allowed.
  • Measuring the time instead of the distance.
  • Not saying that the ruler is caught by the other student.
Part 01.2

Calculate the mass of caffeine taken by group D

📐 Calculation

Given: 32.25 mg of caffeine in 100 cm³

Group D drank: 600 cm³

  1. Find the mass in 1 cm³: 32.25 ÷ 100 = 0.3225 mg
  2. Multiply by 600: 0.3225 × 600 = 193.5 mg

Answer: 193.5 mg

✅ Correct answer

X = 193.5 mg

1 mark for the correct final value.

🧠 Exam technique

  • Use proportional reasoning carefully.
  • Keep units with the calculation.
  • Show the working even for 1 mark — it helps avoid slips.

❌ Common errors

  • Forgetting to scale from 100 cm³ to 600 cm³.
  • Writing the answer as 1935 mg or 19.35 mg.
  • Losing the units.
Part 01.3

Why did group A drink water instead of caffeine drink?

✅ Correct answer

To compare the effect of no caffeine.

Also accepted: it acts as a control group.

💡 Key knowledge

  • A control group gives a baseline to compare against.
  • This shows whether caffeine changes reaction time.

🧠 Exam technique

  • Link your answer to the purpose of a control.
  • Use “compare” or “show the effect of caffeine” for full credit.
Part 01.4

Estimate the reaction time for 23 cm

📐 Calculation

From Table 2:

  • 22 cm = 0.212 s
  • 24 cm = 0.221 s
  1. 23 cm is halfway between 22 cm and 24 cm.
  2. So the reaction time is halfway between 0.212 and 0.221.
  3. Estimate: about 0.217 s.

✅ Correct answer

0.217 s

Accept any value in the range 0.2150 to 0.2180.

🧠 Exam technique

  • Use interpolation: choose the two nearest values.
  • Make sure the answer is in seconds.
  • For this table, a sensible estimate is enough.

❌ Common errors

  • Using 20 cm and 24 cm instead of the nearest values.
  • Not giving a sensible estimate between the two values.
  • Writing the answer in cm instead of seconds.
Part 01.5

Describe the effect of caffeine mass on decrease in reaction time

✅ Correct answer

As the mass of caffeine increases, the decrease in reaction time increases.

You could also say the change in reaction time increases.

💡 Key knowledge

  • Higher caffeine dose generally gives a bigger improvement in reaction time.
  • The graph shows a positive trend overall.

🧠 Exam technique

  • Use the data trend, not just one point.
  • Use comparative words: as... increases, ... increases .

❌ Common errors

  • Saying “caffeine decreases reaction time” without linking to the graph trend.
  • Confusing decrease in reaction time with reaction time itself.
  • Describing only group D or only group A.
Part 01.6

Why was the decrease in reaction time negative for some students?

✅ Correct answer

Their reaction time was greater after the drink.

Allowed: their reaction time was slower / longer after the drink.

💡 Key knowledge

  • A negative decrease means the value went up, not down.
  • So the student reacted more slowly after drinking.

❌ Common errors

  • Calling it an anomalous result — not enough for this mark.
  • Saying “they improved” when the value was negative.
Part 01.7

What is the range of results for group C?

📐 Calculation

From the scatter graph, group C results are about:

  • lowest ≈ 0.01 s
  • highest ≈ 0.18 s

Range = highest − lowest = 0.18 − 0.01 = 0.17 s

✅ Correct answer

0.17 s

Accept values in the range 0.166 to 0.174, depending on reading from the graph.

🧠 Exam technique

  • Range = highest value − lowest value.
  • Read the points carefully from the graph.
  • Units are usually not required here, but it is fine to include s .

❌ Common errors

  • Subtracting the wrong way round is okay if you give the size of the range.
  • Using the spread of all groups instead of just group C.
  • Reading the graph inaccurately.
Part 01.8

Suggest two variables that should be controlled

✅ Correct answers

Any two of the following:

  • same age range
  • same sex / gender
  • same height / weight / BMI
  • all had no caffeine / medication / drugs earlier that day
  • equally tired or same amount of sleep
  • practice of the ruler drop test
  • starting point of ruler / hand
  • same point to take measurement above / below the thumb / finger
  • using the same hand
  • same number of students in each group

💡 Key knowledge

  • Control variables make it a fair test.
  • Only caffeine should change between groups.

🧠 Exam technique

  • Choose variables that could affect reaction time.
  • Write them clearly as specific controls, not vague statements like “keep it the same”.

❌ Common errors

  • Writing “volume of caffeine” or “concentration of caffeine” — these were not accepted as controls here.
  • Repeating the independent variable.
Part 01.9

Explain why the ruler-drop test does not involve a reflex action

✅ Correct answer

It is not automatic because it involves the conscious part of the brain.

Also accepted: it is a voluntary action / involves thinking or decision-making.

💡 Key knowledge

  • Reflexes are automatic.
  • The ruler-drop test needs a decision to catch the ruler.

🧠 Exam technique

  • Compare with a reflex: reflex = automatic, fast, not consciously controlled.
  • Use the word conscious or voluntary for the mark.

❌ Common errors

  • Saying “because it is quick” — speed alone does not make it a reflex.
  • Saying “it uses muscles” — reflexes also use muscles.
  • Calling it a reflex because it is a reaction test.

Quick score booster

💡 What top answers did well

  • Used the correct scientific term: control group, voluntary action, range.
  • Read the table and graph carefully.
  • Gave clear, short answers matched to the marks.

🧠 Best exam habits

  • Use the information given in the question.
  • Show your calculation steps.
  • For graph questions, quote values and describe the trend.

Topics

Biology · B5: Homeostasis and Response

Question and mark scheme from the AQA GCSE Combined Science: Trilogy examination, Biology Paper 2 (Higher), 2019. QuestionVault is an independent revision resource; questions remain the copyright of the awarding body.