AQA GCSE Combined Science: Trilogy Biology Paper 2 (Higher), 2019: Question 1
13 marks · Standard Demand difficulty · Short Answer
Investigate the effect of drinking different amounts of caffeine on reaction time using a ruler-drop test, including method, data interpretation, control variables and why the response is not a reflex action.
Practise this questionQuestion
Question text
01 Some students investigated the effect of drinking caffeine on reaction time.
They used a drink containing 32.25 mg of caffeine per 100 cm3
This is the method used.
1. Divide the students into four groups, A, B, C and D.
2. Measure and record the reaction time of each student using the ruler-drop test.
3. Students in:
• group A drink 200 cm3 of water
• group B drink 200 cm3 of the caffeine drink
• group C drink 400 cm3 of the caffeine drink
• group D drink 600 cm3 of the caffeine drink.
4. Repeat step 2 after 15 minutes.
01.1 Describe how to do the ruler-drop test.
[3 marks]
01.2 Table 1 shows the mass of caffeine taken in by each student.
Table 1
Group Mass of caffeine in mg
A 0
B 64.5
C 129.0
D X
Calculate value X.
*03* [1 mark]
X = mg
01.3 Why did group A drink water instead of the caffeine drink?
[1 mark]
Table 2 was used to convert the results of the ruler-drop test into reaction times.
Table 2
Distance in cm Reaction time in s Distance in cm Reaction time in s
2 0.064 28 0.239
4 0.090 30 0.247
6 0.111 32 0.256
8 0.128 34 0.263
10 0.143 36 0.271
12 0.156 38 0.278
14 0.169 40 0.286
16 0.181 42 0.293
18 0.192 44 0.300
*04* 20 0.202 46 0.306
22 0.212 48 0.313
24 0.221 50 0.319
26 0.230 52 0.326
01.4 Estimate the reaction time for a student who recorded a distance of 23 cm
[1 mark]
Reaction time = s
Students calculated the decrease in their reaction time after the drink compared with
before the drink.
Figure 1 shows the results for each student.
Figure 1
01.5 Describe the effect of the mass of caffeine taken in on the decrease in reaction time.
[1 mark]
01.6 For three students the decrease in reaction time was negative.
Give the reason why the value was negative.
[1 mark]
01.7 What is the range of results for group C?
[1 mark]
01.8 Suggest two variables that should have been controlled in this investigation.
[2 marks]
01.9 Explain why the ruler-drop test does not involve a reflex action.
[2 marks]
Mark scheme
Show the mark scheme
AO /
Question Answers Extra information Mark
Spec. Ref.
01.1 hold a ruler (just) above the ignore near the hand 1 AO1
(open) hand of the other student 4.5.2
drop the ruler and other do not accept give verbal signal 1
student catches it
record where the ruler is caught 1
ignore timing
01.2 193.5 1 AO2
4.5.2
01.3 to compare the effect of no allow as a control (group) 1 AO3
caffeine allow to show the effect of 4.5.2
caffeine
do not accept control variable
01.4 0.217 (s) allow any value in the range 1 AO2
0.2150 to 0.2180 4.5.2
01.5 as mass of caffeine increases allow converse 1 AO3
the decrease / change in 4.5.2
reaction time increases
ignore caffeine decreases
reaction time
do not accept the greater the
increase in reaction time the
greater the mass of caffeine
01.6 their reaction time was greater allow converse 1 AO3
(after the drink) 4.5.2
allow slower / longer for greater
do not accept anomalous result
AO /
Spec. Ref.
01.7 0.01(0) to 0.18(0) 1 AO2
allow values in range 4.5.2
or 0.008 to 0.012
and
0.18(0) to 0.01(0) 0.178 to 0.182
or
0.17(0) 7
allow correct calculation from
values in range
if no values are given, allow
answers in the range 0.166 to
0.174
allow 0.01 ≤ C ≤ 0.18
ignore units
01.8 any two from: 2 AO3
• (same range of) age 4.5.2
• (same) sex / gender
• (same) height / weight / BMI
• all had no
caffeine / medication / drugs
earlier that day
• equally tired or (same)
amount of sleep
• practice of the ruler drop test
• starting point of ruler / hand allow height ruler dropped from
• same point to take
measurement above / below
the thumb / finger
• using the same hand
• (same) number of students in
each group
do not accept volume /
concentration of caffeine
01.9 not automatic allow it is a voluntary action 1 AO2
4.5.2
(because) it involves the allow because it involves 1
(conscious part of the) brain thinking / decision or conscious
action
Total 13
How to answer it
Caffeine and Reaction Time
What this question tests
You need to describe a simple practical method, use a table to calculate caffeine mass, read values from a conversion table, interpret a graph of results, explain the pattern, identify control variables, and give a scientific reason why the ruler-drop test is not a reflex.
Describe how to do the ruler-drop test
✅ Correct answers
- Hold a ruler just above the open hand of the other student.
- Drop the ruler without warning.
- The other student catches it.
- Record where the ruler is caught.
💡 Key knowledge
- The test measures reaction time.
- A shorter catching distance means a faster reaction.
- The same method should be repeated to improve reliability.
🧠 Exam technique
- Write the steps in order.
- Use simple practical language: hold → drop → catch → measure .
- For full marks, describe what the person does, not just “do the test”.
❌ Common errors
- Giving a verbal signal — this was not allowed.
- Measuring the time instead of the distance.
- Not saying that the ruler is caught by the other student.
Calculate the mass of caffeine taken by group D
📐 Calculation
Given: 32.25 mg of caffeine in 100 cm³
Group D drank: 600 cm³
- Find the mass in 1 cm³: 32.25 ÷ 100 = 0.3225 mg
- Multiply by 600: 0.3225 × 600 = 193.5 mg
Answer: 193.5 mg
✅ Correct answer
X = 193.5 mg
🧠 Exam technique
- Use proportional reasoning carefully.
- Keep units with the calculation.
- Show the working even for 1 mark — it helps avoid slips.
❌ Common errors
- Forgetting to scale from 100 cm³ to 600 cm³.
- Writing the answer as 1935 mg or 19.35 mg.
- Losing the units.
Why did group A drink water instead of caffeine drink?
✅ Correct answer
To compare the effect of no caffeine.
💡 Key knowledge
- A control group gives a baseline to compare against.
- This shows whether caffeine changes reaction time.
🧠 Exam technique
- Link your answer to the purpose of a control.
- Use “compare” or “show the effect of caffeine” for full credit.
Estimate the reaction time for 23 cm
📐 Calculation
From Table 2:
- 22 cm = 0.212 s
- 24 cm = 0.221 s
- 23 cm is halfway between 22 cm and 24 cm.
- So the reaction time is halfway between 0.212 and 0.221.
- Estimate: about 0.217 s.
✅ Correct answer
0.217 s
🧠 Exam technique
- Use interpolation: choose the two nearest values.
- Make sure the answer is in seconds.
- For this table, a sensible estimate is enough.
❌ Common errors
- Using 20 cm and 24 cm instead of the nearest values.
- Not giving a sensible estimate between the two values.
- Writing the answer in cm instead of seconds.
Describe the effect of caffeine mass on decrease in reaction time
✅ Correct answer
As the mass of caffeine increases, the decrease in reaction time increases.
💡 Key knowledge
- Higher caffeine dose generally gives a bigger improvement in reaction time.
- The graph shows a positive trend overall.
🧠 Exam technique
- Use the data trend, not just one point.
- Use comparative words: as... increases, ... increases .
❌ Common errors
- Saying “caffeine decreases reaction time” without linking to the graph trend.
- Confusing decrease in reaction time with reaction time itself.
- Describing only group D or only group A.
Why was the decrease in reaction time negative for some students?
✅ Correct answer
Their reaction time was greater after the drink.
💡 Key knowledge
- A negative decrease means the value went up, not down.
- So the student reacted more slowly after drinking.
❌ Common errors
- Calling it an anomalous result — not enough for this mark.
- Saying “they improved” when the value was negative.
What is the range of results for group C?
📐 Calculation
From the scatter graph, group C results are about:
- lowest ≈ 0.01 s
- highest ≈ 0.18 s
Range = highest − lowest = 0.18 − 0.01 = 0.17 s
✅ Correct answer
0.17 s
🧠 Exam technique
- Range = highest value − lowest value.
- Read the points carefully from the graph.
- Units are usually not required here, but it is fine to include s .
❌ Common errors
- Subtracting the wrong way round is okay if you give the size of the range.
- Using the spread of all groups instead of just group C.
- Reading the graph inaccurately.
Suggest two variables that should be controlled
✅ Correct answers
Any two of the following:
- same age range
- same sex / gender
- same height / weight / BMI
- all had no caffeine / medication / drugs earlier that day
- equally tired or same amount of sleep
- practice of the ruler drop test
- starting point of ruler / hand
- same point to take measurement above / below the thumb / finger
- using the same hand
- same number of students in each group
💡 Key knowledge
- Control variables make it a fair test.
- Only caffeine should change between groups.
🧠 Exam technique
- Choose variables that could affect reaction time.
- Write them clearly as specific controls, not vague statements like “keep it the same”.
❌ Common errors
- Writing “volume of caffeine” or “concentration of caffeine” — these were not accepted as controls here.
- Repeating the independent variable.
Explain why the ruler-drop test does not involve a reflex action
✅ Correct answer
It is not automatic because it involves the conscious part of the brain.
💡 Key knowledge
- Reflexes are automatic.
- The ruler-drop test needs a decision to catch the ruler.
🧠 Exam technique
- Compare with a reflex: reflex = automatic, fast, not consciously controlled.
- Use the word conscious or voluntary for the mark.
❌ Common errors
- Saying “because it is quick” — speed alone does not make it a reflex.
- Saying “it uses muscles” — reflexes also use muscles.
- Calling it a reflex because it is a reaction test.
Quick score booster
💡 What top answers did well
- Used the correct scientific term: control group, voluntary action, range.
- Read the table and graph carefully.
- Gave clear, short answers matched to the marks.
🧠 Best exam habits
- Use the information given in the question.
- Show your calculation steps.
- For graph questions, quote values and describe the trend.
Topics
Biology · B5: Homeostasis and Response
Question and mark scheme from the AQA GCSE Combined Science: Trilogy examination, Biology Paper 2 (Higher), 2019. QuestionVault is an independent revision resource; questions remain the copyright of the awarding body.