AQA GCSE Combined Science: Trilogy Biology Paper 1 (Higher), 2020: Question 1

9 marks · Standard Demand difficulty · Short Answer

Investigate osmosis in potato pieces placed in different sugar solution concentrations by identifying the independent variable, explaining mass increase, completing a graph, estimating the isotonic concentration, and calculating percentage change in mass.

Practise this question

Question

The question page shows a biology investigation into the effect of different concentrations of sugar solution on pieces of potato. It includes a six-step method: cut five pieces of potato, record starting mass, place each in a different sugar concentration, remove after 24 hours, record final mass, and calculate change in mass. Part 01.1 asks for the independent variable with four tick-box options; part 01.2 asks why the potato in 0.0 mol/dm³ sugar solution increased in mass. A results table lists sugar concentrations from 0.0 to 0.5 mol/dm³, starting mass, mass after 24 hours, and change in mass; later parts ask students to plot missing points on a graph of change in mass against concentration, draw a best-fit curve, read the concentration where change in mass is zero, and calculate percentage change in mass for the potato in 0.2 mol/dm³ using a given equation.
Question text

01 A student investigated the effect of different concentrations of sugar solution on

pieces of potato.

This is the method used.

1. Cut five pieces of potato.

2. Record the starting mass of each piece of potato.

3. Place each piece of potato in a different concentration of sugar solution.

4. After 24 hours remove the pieces of potato from the solutions.

5. Record the final mass of each piece of potato.

6. Calculate the change in mass for each piece of potato.

01.1 What is the independent variable?

[1 mark]

Tick ( ) one box.

Change in mass of the pieces of potato

Concentration of the sugar solution

Length of time the pieces of potato are in the solution

Starting mass of the pieces of potato4

Table 1 shows the results.

Table 1

Concentration of Mass of potato at Mass of potato Change in mass

sugar solution start in grams after 24 hours in in grams

in mol/dm3 grams

0.0 7.94 10.14 2.20

0.1 7.95 9.10 1.15

0.2 7.96 8.21 0.25

0.3 7.93 7.53 –0.40

0.4 7.93 7.18 –0.75

0.5 7.95 7.00 –0.95

01.2 Explain why the potato in 0.0 mol/dm3 sugar solution increased in mass.

[2 marks]

01.3 Complete Figure 1.

Some of the results have been plotted for you.

You should:

• plot the data from Table 1

• draw a line of best fit through all the points.

[2 marks]

Figure 1

01.4 The mass of a piece of potato does not change when:

concentration of solution inside cells = concentration of solution outside cells

Determine the concentration of sugar solution inside the potato cells.

Use Figure 1.

[1 mark]

Concentration = mol/dm

Table 1 is repeated below.

Table 1

Concentration of Mass of potato at Mass of potato Change in mass

*05* sugar solution start in grams after 24 hours in in grams

in mol/dm3 grams

0.0 7.94 10.14 2.20

0.1 7.95 9.10 1.15

0.2 7.96 8.21 0.25

0.3 7.93 7.53 –0.40

0.4 7.93 7.18 –0.75

0.5 7.95 7.00 –0.95

01.5 Calculate the percentage change in mass for the potato in 0.2 mol/dm3 sugar solution.

Use Table 1.

Use the equation:

change in mass

percentage change in mass = × 100

mass of potato at start

Give your answer to 3 significant figures.

[3 marks]

Percentage change in mass (3 significant figures) = %

Mark scheme

Show the mark scheme The mark scheme is presented in a table with columns for question number, answers, extra information, mark, and AO/specification reference. It gives 01.1 as concentration of the sugar solution; 01.2 awards marks for gained water and osmosis, or an equivalent explanation comparing water concentration inside and outside the potato cells. For 01.3 it awards one mark for correctly plotted points and one for a best-fit curve through all points; 01.4 accepts a graph reading around 0.23 to 0.24 mol/dm³; 01.5 shows the calculation 0.25 × 100 ÷ 7.96 = 3.14% with allowance for equivalent working and correct rounding to 3 significant figures.

AO /

Question Extra information Mark

Answers Spec. Ref.

01.1 concentration of the sugar 1 AO1

solution 4.1.3.2

RPA 2

01.2 gained water 1 AO2

(water moves) by osmosis 1 AO1

or 4.1.3.2

allow converse statements RPA 2

(because) concentration of (because) concentration (of

water outside the potato is sugar solution) inside the potato

greater than inside the cells / is greater than outside the

potato potato / cells

01.3 all points correctly plotted allow ± ½ a square 1 AO2

4.1.3.2

RPA 2

line of best fit drawn as a curve ignore extrapolation of curve 1

through all the points

01.4 correct reading from their graph allow ± ½ a square 1 AO3

4.1.3.2

allow answer in range 0.23 to RPA 2

0.24 (mol/dm3) if no line drawn

0.25 × 100 (8.21-7.96) × 100

01.5 allow 1 AO2

7.96

7.96 4.1.3.2

RPA 2

= 3.14(070352) 1

3.14 (%) allow correct rounding to 3 sig 1

figs of an incorrectly calculated

percentage change

Total 9

How to answer it

Potato Osmosis Investigation

What this question tests

This question checks your understanding of osmosis, identifying the independent variable, reading and plotting data on a graph, finding a value from a graph, and using a formula to calculate percentage change. You must also use correct scientific language and units.

Question 01 overview

This is a standard GCSE Biology practical-style question on osmosis in potato tissue.

💡 Key knowledge

  • Osmosis is the net movement of water through a partially permeable membrane from a region of higher water concentration to lower water concentration.
  • In potato cells, water can move in or out depending on the concentration of the surrounding sugar solution.
  • The independent variable is what the student changes on purpose.

🧠 Exam technique

  • For graph questions, use the data carefully and plot each point accurately.
  • If a line of best fit is needed, draw a smooth curve if the pattern is curved.
  • When calculating percentage change, always use the starting mass in the denominator.

Part (a) 01.1 — Independent variable

✅ Correct answer

Concentration of the sugar solution

1 mark for identifying the variable changed by the student.

💡 Key knowledge

The independent variable is the factor the student deliberately changes between tests. Here, each piece of potato is placed in a different concentration of sugar solution.

❌ Common errors

  • Change in mass is the dependent variable, not the independent variable.
  • Length of time is kept the same, so it is not the independent variable.
  • Starting mass is measured, not changed on purpose.

Part (b) 01.2 — Explain why the potato in 0.0 mol/dm³ increased in mass

✅ Correct answer

It gained water by osmosis because the concentration of water outside the potato was greater than inside the cells.

You could also say: the sugar solution outside was more dilute / had a higher water concentration than inside the potato.

💡 Key knowledge

  • In 0.0 mol/dm³ sugar solution, the outside solution is pure water, so it has a high water concentration.
  • Water moves into the potato cells through the partially permeable cell membranes.
  • As water enters, the potato’s mass increases.

🧠 Exam technique

  • For 2 marks, usually need what happened plus why.
  • Use the word osmosis for one mark and a correct explanation of water concentration for the other.
  • Examiner accepted reverse wording too, as long as the direction of water movement was correct.

❌ Common errors

  • Saying the potato absorbed sugar — the mark scheme is about water.
  • Saying “water moves from low to high concentration” without making clear it is water concentration.
  • Talking about diffusion of sugar instead of osmosis of water.

Part (c) 01.3 — Plot the graph and draw a line of best fit

✅ Correct answer

Plot all the points correctly from Table 1:

  • (0.0, 2.20)
  • (0.1, 1.15)
  • (0.2, 0.25)
  • (0.3, −0.40)
  • (0.4, −0.75)
  • (0.5, −0.95)

Then draw a curve of best fit through the points.

💡 Key knowledge

  • The graph shows a clear downward trend: as sugar concentration increases, change in mass decreases.
  • The line should be a smooth curve, not a zig-zag joining dot to dot.
  • Marks were awarded for accuracy of plotting and for the line of best fit.

🧠 Exam technique

  • Use the same scale as the graph paper and plot carefully to within about ± ½ a square.
  • Do not extrapolate beyond the data unless asked.
  • A good curve should pass close to all points and show the overall pattern.

❌ Common errors

  • Connecting the points with straight line segments instead of drawing a curve.
  • Swapping the axes: concentration is the x-axis, change in mass is the y-axis.
  • Missing a point or plotting it in the wrong place.

Part (d) 01.4 — Find the concentration inside the potato cells

✅ Correct answer

Concentration = 0.23–0.24 mol/dm³

1 mark for a correct reading from the graph. The exact value depends on the line drawn.

💡 Key knowledge

The mass does not change when the concentration inside the potato cells is equal to the concentration outside.

So you find the point where the line of best fit crosses change in mass = 0.

🧠 Exam technique

  • Read from the x-axis where the curve crosses the y = 0 line.
  • Use the graph carefully and quote the answer in mol/dm³.
  • The mark scheme allowed 0.23 or 0.24 mol/dm³ if no line was drawn, showing that a sensible estimate from the table was acceptable.

❌ Common errors

  • Giving the y-value instead of the x-value.
  • Forgetting units.
  • Choosing the wrong crossing point, or using the plotted points instead of the zero-crossing.

Part (e) 01.5 — Calculate the percentage change in mass

📐 Calculations — step by step

Use the values for 0.2 mol/dm³:

  • Change in mass = 0.25 g
  • Mass at start = 7.96 g

Step 1: Write the formula

percentage change in mass = (change in mass ÷ mass at start) × 100

Step 2: Substitute the values

(0.25 ÷ 7.96) × 100

Step 3: Calculate

= 3.14070352...

Step 4: Round to 3 significant figures

3.14%

✅ Correct answer

3.14%

Marks were available for the correct substitution, correct calculation, and correct rounding to 3 significant figures.

🧠 Exam technique

  • Always use the starting mass, not the final mass.
  • Keep the units clear: grams cancel in the calculation, so the final answer is a percentage.
  • Round the final answer to the number of significant figures asked for.

❌ Common errors

  • Using change in mass ÷ final mass instead of starting mass.
  • Forgetting to multiply by 100.
  • Giving a negative percentage when the mass increased.
  • Rounding too early and losing accuracy.

Examiner insight: how marks were awarded

💡 What top answers did well

  • Used precise biological language: osmosis, water concentration, partially permeable membrane.
  • Plotted the graph accurately and drew a smooth curve.
  • Showed calculation working clearly and gave the answer to 3 significant figures.

❌ Where students lost marks

  • Confusing the independent variable with the dependent variable.
  • Explaining mass change using sugar moving instead of water moving.
  • Reading the graph incorrectly or missing the zero-crossing.
  • Using the wrong value in the percentage change formula.

Topics

Biology · Required Practicals · B1: Cell Biology · Biology Required Practicals

Question and mark scheme from the AQA GCSE Combined Science: Trilogy examination, Biology Paper 1 (Higher), 2020. QuestionVault is an independent revision resource; questions remain the copyright of the awarding body.