AQA GCSE Combined Science: Trilogy Biology Paper 1 (Higher), 2022: Question 4
9 marks · Standard Demand difficulty · Short Answer
Answer questions about photosynthesis, including why light is needed, the word equation, planning valid measurements for pondweed, interpreting graph presentation, and estimating the wavelength range of green light.
Practise this questionQuestion
Question text
04 Photosynthesis is an important chemical reaction in plants.
04.1 Why is light needed for photosynthesis?
[1 mark]
04.2 What is the equation for photosynthesis?
[1 mark]
Tick ( ) one box.
C6H12O6 + CO2 → 6 O2 + 6 H2O
CO2 + H2O → CH2O + O2
6 CO2 + 6 H2O → C6H12O6 + 6 O2
6 O2 + 6 H2O → C6H12O6 + 6 CO172
04.3 A student investigated the effect of different colours of light on the rate
of photosynthesis at room temperature.
The student used pondweed in water.
A piece of pondweed was placed in red light, then in blue light and then in green light.
Each colour of light was the same intensity.
Describe how the student should make accurate measurements to obtain valid results
for the rate of photosynthesis.
[4 marks]
*16* 18
A scientist investigated the effect of different wavelengths of light on the rate of
photosynthesis.
The wavelength of light determines the colour of the light.
Figure 8 shows the student’s results.
Figure 9 shows the scientist’s results.
Figure 8
Figure 9
04.4 Why are the results for the two investigations presented differently?
[2 marks]
04.5 Suggest the range in wavelength of green light.
Use Figure 8 and Figure 9.
[1 mark]
Range in wavelength of green light = from nm to nm
Mark scheme
Show the mark scheme
Question 4
AO /
Question Answers Extra information Mark
Spec. Ref.
04.1 to transfer energy allow idea that light is the source 1 AO1
of energy (for the reaction) 4.4.1.1
or do not accept to produce / make
/ create / use energy
photosynthesis is an
endothermic reaction
AO /
Spec. Ref.
04.2 6CO2 + 6H2O → C6H12O6 + 6O2 1 AO1
– OMBINED SCIENCE: TRILOGY – – 4.4.1.1
AO /
Spec. Ref.
04.3 allow the pondweed to allow leave the pondweed in the 1 AO3
equilibrate in the light light (for a few minutes) before
taking measurements
allow use the same
concentration of sodium
hydrogen carbonate solution
ignore control carbon dioxide
concentration unless method
described
ignore use same intensity /
distance of light
ignore control temperature
ignore use same pondweed
use a gas syringe do not accept carbon dioxide 1 AO1
or ignore references to counting
use a (measuring) cylinder to bubbles
18 measure / collect the oxygen /
gas produced
measure time oxygen / gas is 1 AO1
collected for using a timer /
stopwatch / stopclock
repeat the measurements and allow repeat the measurements 1 AO1
calculate a mean and discard anomalies
4.4.1.1
4.4.1.2
– OMBINED SCIENCE: TRILOGY – – RPA5
AO /
Spec. Ref.
04.4 ignore answers relating to the AO2
scale on the y-axis 4.4.1.2
RPA5
independent variable in allow colour of light is a 1
student’s investigation is categoric / discrete variable
categoric / discrete ignore discontinuous
independent variable in allow wavelength is a 1
scientist’s investigation is continuous variable
continuous
AO /
Spec. Ref.
04.5 492 to 577 (nm) allow answers in ranges 1 AO3
475 to 525 and 575 to 650 (nm) 4.4.1.1
4.4.1.2
RPA5
Total Question 4 9
How to answer it
Photosynthesis: light, equation, and investigating rate
Knowing why light is needed for photosynthesis, recognising the correct symbol equation, and describing a fair method for measuring the rate of photosynthesis in pondweed. It also tests how to interpret graphs, identify variables as categorical or continuous, and read a wavelength range from a graph.
Photosynthesis and light
💡 Key knowledge
- Light provides the energy needed for photosynthesis.
- Photosynthesis is an endothermic reaction.
- The word and symbol equation must be memorised.
- Blue and red light usually give a higher rate than green light.
🧠 Exam technique
- Use short, exact science phrases for 1-mark answers.
- For practical methods, give a sequence: set up, measure, time, repeat, mean.
- When reading graphs, use the labelled scale carefully and give the full range asked for.
Part 04.1 — Why is light needed for photosynthesis? [1 mark]
✅ Correct answer
Light transfers energy to the plant for photosynthesis.
Also accepted: photosynthesis is an endothermic reaction.
💡 Key knowledge
- Light is not a raw material in the equation — it is the energy source.
- Endothermic means energy is taken in.
❌ Common errors
- “Light makes glucose” — too vague and not the marking point.
- “Light is used up” — not accepted unless linked to energy transfer.
- “Light creates energy” — incorrect wording.
Part 04.2 — What is the equation for photosynthesis? [1 mark]
✅ Correct answer
6CO₂ + 6H₂O → C₆H₁₂O₆ + 6O₂
💡 Key knowledge
- Reactants: carbon dioxide and water.
- Products: glucose and oxygen.
- This is the balanced symbol equation.
❌ Common errors
- Using the wrong equation with oxygen on the left.
- Missing the coefficients 6.
- Writing CH₂O instead of glucose.
- Forgetting subscripts: use CO₂, H₂O, C₆H₁₂O₆, O₂.
Part 04.3 — Describe how to make accurate measurements and obtain valid results [4 marks]
✅ Correct answer points
- Allow the pondweed to equilibrate in the light before taking measurements.
- Use a gas syringe or a measuring cylinder to measure or collect the oxygen produced.
- Measure the time for which oxygen is collected using a timer/stopwatch.
- Repeat the measurements and calculate a mean.
🧠 Exam technique
- To get full marks, include the idea of measurement of oxygen, time, and repeats.
- Use clear practical language: “collect oxygen”, “measure time”, “calculate a mean”.
- If you mention a control variable, it must be linked to the method to count.
💡 Key knowledge
- Equilibration means letting the plant settle in the new light conditions.
- More oxygen produced in a fixed time = higher rate of photosynthesis.
📐 Suggested 4-mark model answer
Leave the pondweed in the light for a few minutes so it equilibrates. Use a gas syringe or measuring cylinder to collect and measure the oxygen produced. Use a stopwatch to measure the time for each trial. Repeat the measurements several times and calculate a mean.
❌ Common errors
- Only saying “count bubbles” — not the best method here and not what the mark scheme wanted.
- Forgetting to mention timing.
- Not saying repeat/mean.
- Giving general control ideas without describing how measurements are taken.
Part 04.4 — Why were the two investigations presented differently? [2 marks]
✅ Correct answer
- In the student’s investigation, the independent variable is categoric / discrete because the light colour is in separate groups.
- In the scientist’s investigation, the independent variable is continuous because wavelength can take many values.
💡 Key knowledge
- Colour is a category: red, blue, green.
- Wavelength is measured in nm and changes smoothly across a range.
❌ Common errors
- Talking about the y-axis scale — ignored by the mark scheme.
- Saying “discontinuous” instead of discrete; the mark scheme allowed categorical/discrete.
- Calling wavelength a category — incorrect.
Part 04.5 — Suggest the range in wavelength of green light [1 mark]
✅ Correct answer
492 nm to 577 nm
Also accepted as ranges: 475 to 525 nm and 575 to 650 nm when using the graph evidence correctly.
🧠 Exam technique
- Use both Figure 8 and Figure 9 together.
- Read off the wavelength values where green light fits the low-rate region / green band.
- Give units: nm.
❌ Common errors
- Giving only one number instead of a range.
- Forgetting units.
- Using the y-axis values instead of wavelength on the x-axis.
- Mixing up colour names and wavelength values.
📐 How to read the range
- Look at the wavelength axis on Figure 9.
- Identify the region associated with green light.
- Read the lower and upper bounds from the graph.
- Write the answer as a clear range, with units.
What strong answers had in common
💡 Key knowledge
- Light provides energy for photosynthesis.
- The balanced equation is essential recall.
- Practical measurements should be repeatable and fair.
- Colour is categorical; wavelength is continuous.
🧠 Exam technique
- Be precise: use “oxygen produced”, not just “gas”.
- Always include units when reading graphs.
- For practicals, write enough steps to show how valid data is obtained.
❌ Final traps to avoid
- Wrong equation order.
- No repeat or mean in the method.
- Mixing up colour and wavelength.
- Not matching the graph to the question asked.
Topics
Biology · Required Practicals · B4: Bioenergetics · Biology Required Practicals
Question and mark scheme from the AQA GCSE Combined Science: Trilogy examination, Biology Paper 1 (Higher), 2022. QuestionVault is an independent revision resource; questions remain the copyright of the awarding body.