AQA GCSE Combined Science: Trilogy Biology Paper 1 (Higher), 2022: Question 4

9 marks · Standard Demand difficulty · Short Answer

Answer questions about photosynthesis, including why light is needed, the word equation, planning valid measurements for pondweed, interpreting graph presentation, and estimating the wavelength range of green light.

Practise this question

Question

The question page is labelled Question 04 and contains five parts about photosynthesis. Part 04.1 asks why light is needed for photosynthesis for 1 mark, and part 04.2 asks students to tick the correct balanced equation for photosynthesis from four options, including the correct option 6CO2 + 6H2O → C6H12O6 + 6O2. Part 04.3 describes a pondweed experiment in red, blue and green light of the same intensity and asks how accurate measurements should be made to obtain valid results for the rate of photosynthesis, with lined space for a 4-mark written response. Below this, Figure 8 is a bar chart of rate of photosynthesis against colour of light, showing red at about 7.5 units, blue at about 9 units and green near 0.5 units, and Figure 9 is a line graph of rate of photosynthesis against wavelength from 400 to 700 nm, with peaks in the blue and red regions and a trough around the green region; parts 04.4 and 04.5 ask why the two investigations are presented differently and to suggest the wavelength range of green light using both figures.
Question text

04 Photosynthesis is an important chemical reaction in plants.

04.1 Why is light needed for photosynthesis?

[1 mark]

04.2 What is the equation for photosynthesis?

[1 mark]

Tick ( ) one box.

C6H12O6 + CO2 → 6 O2 + 6 H2O

CO2 + H2O → CH2O + O2

6 CO2 + 6 H2O → C6H12O6 + 6 O2

6 O2 + 6 H2O → C6H12O6 + 6 CO172

04.3 A student investigated the effect of different colours of light on the rate

of photosynthesis at room temperature.

The student used pondweed in water.

A piece of pondweed was placed in red light, then in blue light and then in green light.

Each colour of light was the same intensity.

Describe how the student should make accurate measurements to obtain valid results

for the rate of photosynthesis.

[4 marks]

*16* 18

A scientist investigated the effect of different wavelengths of light on the rate of

photosynthesis.

The wavelength of light determines the colour of the light.

Figure 8 shows the student’s results.

Figure 9 shows the scientist’s results.

Figure 8

Figure 9

04.4 Why are the results for the two investigations presented differently?

[2 marks]

04.5 Suggest the range in wavelength of green light.

Use Figure 8 and Figure 9.

[1 mark]

Range in wavelength of green light = from nm to nm

Mark scheme

Show the mark scheme The mark scheme is presented as tables with columns for Question, Answers, Extra information, Mark, and AO/Spec Ref. For 04.1 it gives ‘to transfer energy’ or that photosynthesis is an endothermic reaction; for 04.2 it gives the balanced equation 6CO2 + 6H2O → C6H12O6 + 6O2. For 04.3 it awards marks for allowing pondweed to equilibrate in the light, measuring oxygen produced using a gas syringe or measuring cylinder, timing collection with a stopwatch or timer, and repeating measurements to calculate a mean; extra notes allow sodium hydrogencarbonate and reject counting bubbles. For 04.4 it states the student’s independent variable is categorical or discrete while the scientist’s is continuous, and for 04.5 it gives a green light wavelength range of 492 to 577 nm, with some alternative ranges allowed; the total for Question 4 is 9 marks.

Question 4

AO /

Question Answers Extra information Mark

Spec. Ref.

04.1 to transfer energy allow idea that light is the source 1 AO1

of energy (for the reaction) 4.4.1.1

or do not accept to produce / make

/ create / use energy

photosynthesis is an

endothermic reaction

AO /

Spec. Ref.

04.2 6CO2 + 6H2O → C6H12O6 + 6O2 1 AO1

– OMBINED SCIENCE: TRILOGY – – 4.4.1.1

AO /

Spec. Ref.

04.3 allow the pondweed to allow leave the pondweed in the 1 AO3

equilibrate in the light light (for a few minutes) before

taking measurements

allow use the same

concentration of sodium

hydrogen carbonate solution

ignore control carbon dioxide

concentration unless method

described

ignore use same intensity /

distance of light

ignore control temperature

ignore use same pondweed

use a gas syringe do not accept carbon dioxide 1 AO1

or ignore references to counting

use a (measuring) cylinder to bubbles

18 measure / collect the oxygen /

gas produced

measure time oxygen / gas is 1 AO1

collected for using a timer /

stopwatch / stopclock

repeat the measurements and allow repeat the measurements 1 AO1

calculate a mean and discard anomalies

4.4.1.1

4.4.1.2

– OMBINED SCIENCE: TRILOGY – – RPA5

AO /

Spec. Ref.

04.4 ignore answers relating to the AO2

scale on the y-axis 4.4.1.2

RPA5

independent variable in allow colour of light is a 1

student’s investigation is categoric / discrete variable

categoric / discrete ignore discontinuous

independent variable in allow wavelength is a 1

scientist’s investigation is continuous variable

continuous

AO /

Spec. Ref.

04.5 492 to 577 (nm) allow answers in ranges 1 AO3

475 to 525 and 575 to 650 (nm) 4.4.1.1

4.4.1.2

RPA5

Total Question 4 9

How to answer it

Photosynthesis: light, equation, and investigating rate

What this question tests
Knowing why light is needed for photosynthesis, recognising the correct symbol equation, and describing a fair method for measuring the rate of photosynthesis in pondweed. It also tests how to interpret graphs, identify variables as categorical or continuous, and read a wavelength range from a graph.
Question 04 overview

Photosynthesis and light

💡 Key knowledge

  • Light provides the energy needed for photosynthesis.
  • Photosynthesis is an endothermic reaction.
  • The word and symbol equation must be memorised.
  • Blue and red light usually give a higher rate than green light.

🧠 Exam technique

  • Use short, exact science phrases for 1-mark answers.
  • For practical methods, give a sequence: set up, measure, time, repeat, mean.
  • When reading graphs, use the labelled scale carefully and give the full range asked for.

Part 04.1 — Why is light needed for photosynthesis? [1 mark]

✅ Correct answer

Light transfers energy to the plant for photosynthesis.

Also accepted: photosynthesis is an endothermic reaction.

💡 Key knowledge

  • Light is not a raw material in the equation — it is the energy source.
  • Endothermic means energy is taken in.

❌ Common errors

  • “Light makes glucose” — too vague and not the marking point.
  • “Light is used up” — not accepted unless linked to energy transfer.
  • “Light creates energy” — incorrect wording.
Examiner note: Students gained the mark by stating that light transfers energy or by naming photosynthesis as an endothermic reaction. The key idea is energy input.

Part 04.2 — What is the equation for photosynthesis? [1 mark]

✅ Correct answer

6CO₂ + 6H₂O → C₆H₁₂O₆ + 6O₂

💡 Key knowledge

  • Reactants: carbon dioxide and water.
  • Products: glucose and oxygen.
  • This is the balanced symbol equation.

❌ Common errors

  • Using the wrong equation with oxygen on the left.
  • Missing the coefficients 6.
  • Writing CH₂O instead of glucose.
  • Forgetting subscripts: use CO₂, H₂O, C₆H₁₂O₆, O₂.
Examiner note: Only one option was correct. The best responses recognised the balanced equation exactly, including the correct placement of oxygen as a product.

Part 04.3 — Describe how to make accurate measurements and obtain valid results [4 marks]

✅ Correct answer points

  • Allow the pondweed to equilibrate in the light before taking measurements.
  • Use a gas syringe or a measuring cylinder to measure or collect the oxygen produced.
  • Measure the time for which oxygen is collected using a timer/stopwatch.
  • Repeat the measurements and calculate a mean.

🧠 Exam technique

  • To get full marks, include the idea of measurement of oxygen, time, and repeats.
  • Use clear practical language: “collect oxygen”, “measure time”, “calculate a mean”.
  • If you mention a control variable, it must be linked to the method to count.

💡 Key knowledge

  • Equilibration means letting the plant settle in the new light conditions.
  • More oxygen produced in a fixed time = higher rate of photosynthesis.

📐 Suggested 4-mark model answer

Leave the pondweed in the light for a few minutes so it equilibrates. Use a gas syringe or measuring cylinder to collect and measure the oxygen produced. Use a stopwatch to measure the time for each trial. Repeat the measurements several times and calculate a mean.

❌ Common errors

  • Only saying “count bubbles” — not the best method here and not what the mark scheme wanted.
  • Forgetting to mention timing.
  • Not saying repeat/mean.
  • Giving general control ideas without describing how measurements are taken.
Examiner note: Top answers had a clear method for measuring oxygen, a time measurement, and a repeat-and-mean step. The mark scheme also allowed equilibration in the light before measuring.

Part 04.4 — Why were the two investigations presented differently? [2 marks]

✅ Correct answer

  • In the student’s investigation, the independent variable is categoric / discrete because the light colour is in separate groups.
  • In the scientist’s investigation, the independent variable is continuous because wavelength can take many values.

💡 Key knowledge

  • Colour is a category: red, blue, green.
  • Wavelength is measured in nm and changes smoothly across a range.

❌ Common errors

  • Talking about the y-axis scale — ignored by the mark scheme.
  • Saying “discontinuous” instead of discrete; the mark scheme allowed categorical/discrete.
  • Calling wavelength a category — incorrect.
Examiner note: The key distinction was whether the independent variable was a set of labels (colour) or a continuous measurement (wavelength). This is why the results were shown differently.

Part 04.5 — Suggest the range in wavelength of green light [1 mark]

✅ Correct answer

492 nm to 577 nm

Also accepted as ranges: 475 to 525 nm and 575 to 650 nm when using the graph evidence correctly.

🧠 Exam technique

  • Use both Figure 8 and Figure 9 together.
  • Read off the wavelength values where green light fits the low-rate region / green band.
  • Give units: nm.

❌ Common errors

  • Giving only one number instead of a range.
  • Forgetting units.
  • Using the y-axis values instead of wavelength on the x-axis.
  • Mixing up colour names and wavelength values.

📐 How to read the range

  1. Look at the wavelength axis on Figure 9.
  2. Identify the region associated with green light.
  3. Read the lower and upper bounds from the graph.
  4. Write the answer as a clear range, with units.
Examiner note: The mark was for a sensible wavelength range in nanometres. Exact wording mattered less than using the graph correctly and giving a valid interval.

What strong answers had in common

💡 Key knowledge

  • Light provides energy for photosynthesis.
  • The balanced equation is essential recall.
  • Practical measurements should be repeatable and fair.
  • Colour is categorical; wavelength is continuous.

🧠 Exam technique

  • Be precise: use “oxygen produced”, not just “gas”.
  • Always include units when reading graphs.
  • For practicals, write enough steps to show how valid data is obtained.

❌ Final traps to avoid

  • Wrong equation order.
  • No repeat or mean in the method.
  • Mixing up colour and wavelength.
  • Not matching the graph to the question asked.

Topics

Biology · Required Practicals · B4: Bioenergetics · Biology Required Practicals

Question and mark scheme from the AQA GCSE Combined Science: Trilogy examination, Biology Paper 1 (Higher), 2022. QuestionVault is an independent revision resource; questions remain the copyright of the awarding body.