AQA GCSE Combined Science: Trilogy Biology Paper 1 (Foundation), 2023: Question 3

8 marks · Standard Demand difficulty · Short Answer

Analyze water loss in plants through transpiration, including calculating mass loss from a table, interpreting a rate from a graph, and predicting the effect of environmental factors.

Practise this question

Question

The image shows a multi-part GCSE biology question. Figure 4 depicts a potted plant with a plastic bag sealed around the pot, sitting on a digital balance reading 510.7g. Table 1 lists time in hours (0 to 5), mass in grams, and total mass of water lost, with one missing mass value 'X' at 5 hours. Figure 5 is a line graph showing a linear relationship between time and total mass of water lost, reaching 9.0g at 5 hours. The questions ask for the source of water for photosynthesis, the term for water evaporation from leaves, a calculation for mass X, identifying the rate of water loss, drawing a new line for a lower temperature, and suggesting a way to increase the rate of water loss.
Question text

03 Plants need water for photosynthesis.

03.1 Where do plants obtain water for photosynthesis from?

[1 mark]

Plants lose water from their leaves through small pores called stomata.

03.2 What is the evaporation of water from leaves called?

[1 mark]

Tick ( ) one box.

Active transport

Respiration

Transpiration 14

A student investigated the mass of water lost from a plant.

Figure 4 shows the apparatus.

Figure 4

*13* This is the method used.

1. Seal a plastic bag around the pot of a potted plant.

2. Place the plant on a balance in a room at 20 °C.

3. Record the mass.

4. Record the mass every hour for 5 hours.

5. Calculate the total mass of water lost from the plant after each hour.

Table 1 shows the results.

Table 1

Total mass of water

Time in hours Mass in grams

lost in grams

0 510.7 0.0

1 508.9 1.8

2 507.1 3.6

3 505.3 5.4

4 503.5 7.2

5 X 9.0

03.3 Calculate mass X in Table 1.

[2 marks]

Mass X = grams

Figure 5 shows the results.

Figure 5

03.4 What was the rate of water loss from the plant?

[1 mark]

Tick ( ) one box.

0.9 grams/hour

1.8 grams/hour

9.0 grams/hour

03.5 The investigation was repeated at a lower temperature.

Draw one line on Figure 5 to show how the results would be different at a

lower temperature.

[2 marks]

03.6 Suggest one change to the investigation that would increase the rate of water loss

from the plant.

Do not refer to temperature in your answer.

[1 mark]

Mark scheme

Show the mark scheme The mark scheme provides the following answers: 03.1 accepts 'soil' or 'ground'; 03.2 is 'transpiration'; 03.3 requires the calculation 510.7 - 9.0 to get 501.7 grams; 03.4 is '1.8 grams/hour'; 03.5 requires a straight line from the origin (0,0) to 5 hours with a less steep gradient than the original; 03.6 accepts 'increase air movement', 'increase light intensity', or 'decrease humidity'. Specification references 4.4.1.1 and 4.2.3.2 are cited.

Question 3

AO /

Question Answers Extra information Mark

Spec. Ref.

03.1 soil allow ground 1 AO1

ignore air 4.4.1.1

ignore rain 4.2.3.2

ignore roots

AO /

Spec. Ref.

03.2 transpiration 1 AO1

4.2.3.2

AO /

Spec. Ref.

03.3 510.7 – 9(.0) 1 AO2

or 4.2.3.2

503.5 – 1.8

501.7 (grams) 1

AO /

Spec. Ref.

03.4 1.8 grams/hour 1 AO2

4.2.3.2

AO /

Spec. Ref.

03.5 straight line drawn from 0,0 to 5 ignore any extrapolations 1 AO2

hours

at a less steep gradient below 1 AO3

the line on Figure 5

4.2.3.2

AO /

Spec. Ref.

03.6 ignore references to AO3

temperature 4.2.3.2

ignore references to the bag

any one from: 1

• increase air movement allow descriptions of how

• increase light intensity changes could be achieved

• decrease humidity experimentally eg use a fan

Total Question 3 8

How to answer it

Plant Transport and Transpiration

What this question tests

This question assesses your understanding of the transpiration stream. You need to know how plants obtain water, the process of evaporation from leaves, how to interpret experimental data from a balance, and the factors that change the rate of water loss.

Part 03.1

Water Uptake

Correct Answer

Soil (or ground)

Key Knowledge

  • Water is absorbed from the soil by root hair cells.
  • This happens via osmosis.

Common Errors

Do not say "rain" or "the air". While rain provides water to the soil, the plant specifically obtains it from the soil.

Part 03.2

Defining the Process

Correct Answer

Transpiration

Exam Technique

This is a "Tick one box" question. If you tick more than one, you will get 0 marks even if one of them is correct. Read the options carefully!

What is Transpiration?

It is the evaporation of water from the surface of the leaf (mostly through stomata) and the subsequent movement of water through the plant in the xylem.

Part 03.3

Calculating Mass Loss

Finding the value of X in Table 1

Step-by-Step Calculation

  1. Identify the starting mass: At 0 hours, the mass was 510.7 g .
  2. Identify the total loss: At 5 hours, the "Total mass of water lost" is 9.0 g .
  3. Subtract: 510.7 - 9.0 = 501.7

Mass X = 501.7 grams

Mark Breakdown

1 Mark for the correct subtraction method.

1 Mark for the correct final answer.

Common Trap

Students often try to subtract the previous hour's mass (503.5) from the starting mass. Always check the column headings to see if you are looking at "Total Loss" or "Mass in grams".

Part 03.4

Rate of Water Loss

Correct Answer

1.8 grams/hour

How to find it

Look at the "Total mass of water lost" column. Every hour, the loss increases by exactly 1.8g (e.g., 1.8, 3.6, 5.4...). This is the rate.

Part 03.5

Graphing Variables

Effect of Lower Temperature

What to draw

You must draw a straight line starting at the origin (0,0) that is less steep than the original line (it should sit below the original line on the graph).

The Science

Lower temperatures mean water molecules have less kinetic energy, so evaporation happens more slowly. A slower rate of loss results in a shallower gradient on the graph.

Examiner Tip

Use a ruler! Many students lose marks for "sketchy" or curved lines when the data shows a constant rate (a straight line).

Part 03.6

Increasing Transpiration

Correct Answers (Choose one)

  • Increase air movement (e.g., use a fan)
  • Increase light intensity (e.g., move the lamp closer)
  • Decrease humidity

The "Forbidden" Answer

The question explicitly says: "Do not refer to temperature". If you write "increase temperature", you will get 0 marks, even though it is scientifically true.

Why these work

Light causes stomata to open wider for photosynthesis, allowing more water to escape. Wind moves water vapour away from the leaf surface, maintaining a steep concentration gradient.

Topics

Biology · B4: Bioenergetics · B2: Organisation

Question and mark scheme from the AQA GCSE Combined Science: Trilogy examination, Biology Paper 1 (Foundation), 2023. QuestionVault is an independent revision resource; questions remain the copyright of the awarding body.