AQA GCSE Combined Science: Trilogy Biology Paper 1 (Higher), 2023: Question 4

9 marks · Standard Demand difficulty · Short Answer

Identify leaf tissues, explain adaptations of spongy mesophyll and xylem, and describe how guard cells open the stoma.

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Question

A biology exam question sheet shows Figure 4, a diagram of a leaf cross-section with tissues labelled A, B, C and D. The top layer is the epidermis, below it are elongated palisade cells, then irregular spongy mesophyll cells with large air spaces, and a vascular bundle in the middle; guard cells are shown at the lower surface. Question 04.1 asks which tissue is the epidermis and provides four tick boxes A, B, C and D. Question 04.2 asks how the spongy mesophyll is adapted for its function, using Figure 4. Question 04.3 asks how xylem is adapted to transport water through a plant. Figure 5 shows a pair of guard cells around a stoma, with labels for the thinner part of the cell wall, thicker part of the cell wall, and the stoma. Question 04.4 asks how an increase in glucose concentration in the guard cells causes the stoma to open.
Question text

04 A leaf is a plant organ.

Figure 4 shows tissues in a leaf.

Figure 4

04.1 Which tissue is the epidermis?

[1 mark]

Tick ( ) one box.

A B 13C D

04.2 Explain how the spongy mesophyll is adapted for its function.

Use Figure 4.

[3 marks]

04.3 The xylem is adapted to transport water through a plant.

Explain one way that xylem is adapted for its function.

[2 marks]

04.4 Figure 5 shows a pair of guard cells around a stoma.

Figure 5

During the day, glucose is made in the guard cells.

*13* Describe how an increase in glucose concentration in the guard cells causes the

stoma to open.

[2 marks]

Mark scheme

Show the mark scheme Mark scheme for AQA GCSE Combined Science: Trilogy Biology Paper 1 (Higher), 2023: Question 4

Question 4

AO /

Question Answers Extra information Mark

Spec. Ref.

04.1 A 1 AO1

4.2.1

4.2.3.1

AO /

Spec. Ref.

04.2 has (large) air spaces (between allow has (large) gaps (between 1 AO1

cells) cells) 4.2.1

4.2.3.1

(so) gases can diffuse / move allow carbon dioxide or oxygen 1

through the leaf or water vapour for gases

to / from the photosynthetic / allow to / from the upper 1

palisade / B layer mesophyll

if named gas given for mp 2

direction of movement must be

correct

if no other marks awarded allow

1 mark for cells have

chloroplasts for photosynthesis

AO /

Spec. Ref.

reason must correctly match the

04.3 structure AO1

4.2.1

composed of hollow tubes / cells allow cells are empty 1 4.2.3.1

allow no cytoplasm / nucleus / 4.2.3.2

sub-cellular structures

(so) no obstruction to water flow allow (so) water flows easily if 1

linked to hollow tubes

allow to maintain the

transpiration stream

OR

has lignin (1) ignore has thick walls

(to) hold tubes open (1) allow (to) keep water inside

or xylem

(to) strengthen the tubes allow (to) make tubes rigid

OR

no end walls (1)

(so) water flows easily (1) must be linked to an adaptation

or

(so) water flows continuously allow (so) maintains a constant

water flow

allow to maintain the

transpiration stream

AO /

Spec. Ref.

04.4 water enters (guard) cells by allow water enters (guard) cells 1 AO2

osmosis because the concentration of

water is higher outside the cell

than inside the cell

cells swell and curve (to open allow cells become turgid and 1 AO3

the stoma) curve (to open the stoma) 17

4.1.1.2

allow inner wall of (guard) cell is 4.1.1.3

thicker / stronger or less flexible 4.1.3.2

than outer wall so cells curve (to 4.2.3.1

open stoma) 4.2.3.2

Total Question 4 8

How to answer it

Leaf Tissues, Xylem and Stomata

What this question tests

This question tests your ability to: identify leaf tissues from a diagram, explain how spongy mesophyll helps gas exchange, explain how xylem is adapted for water transport, and describe how guard cells open a stoma by osmosis and cell turgor.

It is mainly recall + explanation with simple biology vocabulary. Marks are awarded for clear matching of structure to function.

Question 04.1–04.4

Leaf tissues and stomata

Short title: Identifying leaf tissues and explaining transport and gas exchange

Part (a) 04.1 — Which tissue is the epidermis?

✅ Correct answer

A

1 mark for correctly identifying the epidermis in Figure 4.

💡 Key knowledge

  • The upper epidermis is the outer layer at the top of the leaf.
  • It is above the palisade mesophyll.
  • In diagrams, it is usually shown as the top row of cells.

🧠 Exam technique

  • Look for the top layer of cells.
  • If the question says “tick one box”, only one answer is needed.
  • Use the diagram labels carefully — the examiner wants the tissue, not the function.

❌ Common errors

  • Choosing the palisade layer because it is also near the top.
  • Confusing epidermis with guard cells.
  • Not matching the label to the actual position in the figure.

Part (b) 04.2 — Explain how the spongy mesophyll is adapted for its function

✅ Correct answer

Any three of the following ideas score the 3 marks:

  • It has large air spaces between the cells.
  • This allows gases to diffuse/move through the leaf.
  • This helps gases move to and from the photosynthetic palisade layer.
Marks are for linking the adaptation to gas exchange and photosynthesis.

💡 Key knowledge

  • Spongy mesophyll cells are loosely packed.
  • The gaps let oxygen, carbon dioxide and water vapour move easily.
  • Gas movement happens by diffusion.
  • The gases need to reach the palisade mesophyll where most photosynthesis happens.

🧠 Exam technique

  • Use the phrase “large air spaces” — this is the key structural feature.
  • Then explain the function: “so gases can diffuse through the leaf”.
  • Finish by linking to photosynthesis: “to/from the palisade layer”.
  • That structure → function chain is exactly what earns marks.

❌ Common errors

  • Saying only “it has air spaces” without explaining why they matter.
  • Talking about chloroplasts here — that is not the main marking point.
  • Writing “oxygen goes in for photosynthesis” instead of carbon dioxide.
  • Missing the direction: gases move to and from the palisade layer.

📐 Model 3-mark response

“The spongy mesophyll has large air spaces between the cells, so gases can diffuse through the leaf, to and from the palisade mesophyll where photosynthesis happens.”

This would typically gain all 3 marks because it states the adaptation, the process, and the link to function.

Part (c) 04.3 — Explain one way xylem is adapted for its function

✅ Correct answer

Any one developed explanation from the mark scheme can score 2 marks:

  • Xylem is composed of hollow tubes/cells with no cytoplasm or nucleus, so there is no obstruction to water flow.
  • OR xylem contains lignin, which holds tubes open / strengthens them.
  • OR xylem has no end walls, so water flows easily/continuously.

💡 Key knowledge

  • Xylem transports water and mineral ions from roots to leaves.
  • It is made of dead cells joined end to end to form tubes.
  • Lignin is a strong waterproof material that supports the vessel.

🧠 Exam technique

  • You need structure + reason for full marks.
  • For example: hollow tubes → less resistance → water flows easily .
  • Or: lignin → strengthens walls → keeps xylem open .
  • The examiner will not award both marks for just saying “it transports water”.

❌ Common errors

  • Saying only “xylem carries water” — too vague for 2 marks.
  • Writing “thick walls” without mentioning lignin or why thick walls help.
  • Forgetting to link the feature to easy water flow or support.
  • Mixing up xylem with phloem.

📐 Full-mark examples

Example 1: “Xylem is made of hollow tubes with no cytoplasm, so water can flow through with no obstruction.”

Example 2: “Xylem contains lignin, which strengthens the tubes and keeps them open.”

Each example gives a structure and a function, which is exactly what the mark scheme wants.

Part (d) 04.4 — How glucose causes the stoma to open

✅ Correct answer

  • Water enters the guard cells by osmosis.
  • The cells become turgid, so they swell and curve, opening the stoma.
2 marks: one for osmosis, one for the guard cells swelling/curving to open the pore.

💡 Key knowledge

  • During the day, glucose is made in guard cells by photosynthesis.
  • More glucose means a lower water potential in the guard cells.
  • Water moves in by osmosis from a region of higher water concentration.
  • The inner wall of each guard cell is thicker/less flexible, so the cells bend apart when turgid.

🧠 Exam technique

  • Use the sequence: glucose increases → water enters by osmosis → cells become turgid → stoma opens.
  • If you mention the wall thickness, make it clear that this causes the cells to curve apart.
  • Keep the process in the correct order — this is how marks are awarded.

❌ Common errors

  • Saying “glucose opens the stomata” without explaining how.
  • Forgetting osmosis.
  • Saying water enters by diffusion — this is wrong here.
  • Not mentioning that the guard cells swell/curve.

📐 Step-by-step answer

  1. Glucose concentration increases in the guard cells.
  2. This lowers the water concentration in the guard cells.
  3. Water enters the guard cells by osmosis.
  4. The guard cells become turgid.
  5. Because the inner walls are thicker, the cells curve and the stoma opens.
A clear sequence like this shows cause and effect, which is ideal for 2-mark explanation questions.

Examiner insight: what got the marks?

💡 What top answers did well

  • Used accurate biology terms: diffuse, osmosis, turgid, lignin, hollow tubes.
  • Linked structure to function clearly.
  • Kept answers short but specific.

❌ Where students lost marks

  • Being too vague: “helps transport” without saying how.
  • Mixing up tissues and functions.
  • Missing the key words that the mark scheme expects.

🧠 Best exam habit

  • Always ask: What is the structure? What is the function?
  • For 2 marks, try to give one structure + one explanation.
  • For 3 marks, give a complete chain of reasoning.

Quick revision summary

✅ Answers in one line

04.1: A

04.2: Large air spaces → gases diffuse through leaf → to/from palisade mesophyll.

04.3: Xylem is hollow / lignified / has no end walls, so water flows easily.

04.4: Water enters by osmosis, guard cells become turgid and curve, opening the stoma.

🧠 Final tip

In GCSE Biology, the examiner is usually rewarding clear biological explanation, not long paragraphs. Use the key terms, stay focused on the question, and match the tissue to its job.

Topics

Biology · B2: Organisation · B4: Bioenergetics

Question and mark scheme from the AQA GCSE Combined Science: Trilogy examination, Biology Paper 1 (Higher), 2023. QuestionVault is an independent revision resource; questions remain the copyright of the awarding body.