AQA GCSE Combined Science: Trilogy Biology Paper 1 (Higher), 2024: Question 2
9 marks · Standard Demand difficulty · Short Answer
Investigate how the concentration of salt solution affects the change in mass of tubing in an osmosis experiment and use a graph to determine the concentration of solution Z.
Practise this questionQuestion
Question text
02 A student investigated the concentration of salt in solution Z.
The student used a method involving osmosis.
The student used tubing made of partially permeable membrane.
This is the method used.
1. Cut six pieces of tubing to the same length.
2. Tie one end of each piece of tubing.
3. Put the same volume of solution Z into each piece of tubing.
4. Tie the other end of each piece of tubing to form a sealed tube.
5. Record the mass of each tube.
6. Place each tube into a different concentration of salt solution.
7. After 2 hours, remove each tube from the salt solutions.
8. Record the mass of each tube.
Figure 1 shows one of the sealed tubes in a salt solution.
Figure 1
02.1 What was the independent variable for the investigation?
[1 mark]
Tick ( ) one box.
Change in mass of tube
Concentration of salt solution
Time in salt solution
Volume of solution Z
02.2 The student dried the outside of each tube with a paper towel before
recording the mass.
Why was it important to dry the tubes?
[1 mark]
Table 1 shows the results.
Table 1
Concentration of Mass of tube in grams
Percentage (%)
salt solution
3 change in mass
in mol/dm At start After 2 hours Change
0.0 15.54 16.50 0.96 X
0.2 15.16 15.78 0.62 4.1
0.4 15.00 15.35 0.35 2.3
0.6 15.29 15.37 0.08 0.5
0.8 14.95 14.75 –0.20 –1.3
1.0 14.77 14.40 –0.37 –2.5
02.3 Calculate value X in Table 1.
*07* Give your answer to 1 decimal place.
[3 marks]
Value X (1 decimal place) = %
02.4 Complete Figure 2.
You should:
• plot the percentage change in mass from Table 1 for salt concentrations of
only 0.2 mol/dm3 to 1.0 mol/dm3
• draw a line of best fit.
One of the results has been plotted for you.9
[3 marks]
Figure 2
02.5 Determine the concentration of salt in solution Z.
Use Figure 2.
[1 mark]
Concentration = mol/dm3
Mark scheme
Show the mark scheme
Question 2
AO /
Question Answers Extra information Mark
Spec. Ref.
02.1 concentration of salt solution 1 AO2
4.1.3.2
RPA2
AO /
Spec. Ref.
02.2 (water / solution on the tube / allow the results would not be 1 AO2
tubing) would affect / increase valid 4.1.3.2
the mass RPA2
AO /
Spec. Ref.
02.3 0.96 1 AO2
× 100 4.1.3.2
15.54
RPA2
6.17 (760618…) (%) 1
6.2 (%) allow answer written in Table 1 1
allow correct conversion to one
decimal place from students’
incorrect percentage change
calculation using figures from
0.0 Concentration of salt
solution in mol/dm3
AO /
Spec. Ref.
02.4 all 4 points plotted correctly allow a tolerance of ± ½ a small 2 AO2
square
allow 1 mark for 3 points plotted
correctly
ignore attempt to plot a point for
0 mol/dm3
line of best fit ignore extrapolation 1 AO3
4.1.3.2
– – – RPA2
AO /
Spec. Ref.
02.5 value from student’s line of best allow a tolerance of ± ½ a small 1 AO3
fit square 4.1.3.2
RPA2 9
if no line of best fit drawn allow 1
mark for an answer in the range
0.64 to 0.66
Total Question 2 9
How to answer it
Osmosis in Visking Tubing
What this question tests
This question tests your understanding of osmosis, variables in an investigation, why fair measurements matter, how to calculate percentage change in mass, how to plot a graph correctly, and how to use a line of best fit to estimate the concentration of a solution.
This is a practical-style biology question about osmosis using partially permeable tubing. To score well, you need to:
- identify the independent variable
- explain how to make the results valid
- calculate percentage change in mass
- plot points accurately and draw a line of best fit
- find where the graph crosses 0% change in mass
Part (a) – Independent variable
Question 02.1
✅ Correct answer
Concentration of salt solution
💡 Key knowledge
- The independent variable is the one the student changes.
- Here, each tube was placed in a different concentration of salt solution.
- The dependent variable was the change in mass of the tube.
🧠 Exam technique
Look for what is deliberately changed between trials. If the question says “placed each tube into a different concentration”, that is your clue.
❌ Common errors
- Choosing change in mass – that is the result measured, so it is the dependent variable.
- Choosing time in salt solution – this stayed the same at 2 hours.
- Choosing volume of solution Z – the same volume was put in each tube.
Part (b) – Why dry the tubes?
Question 02.2
✅ Correct answer
Any water / salt solution on the outside of the tube would affect or increase the mass.
A clear alternative: so the mass measured is only the mass of the tube and its contents.
💡 Key knowledge
- You must remove liquid from the outside before weighing.
- Otherwise, the balance measures the tube plus extra liquid stuck on the surface.
- That makes the results not valid.
🧠 Exam technique
For a 1-mark practical question, make the point directly: liquid on the outside would change the mass reading.
❌ Common errors
- Saying only “to make it fair” without explaining how.
- Talking about stopping osmosis – drying the outside does not affect osmosis inside the tube.
- Forgetting to mention mass or validity.
Part (c) – Calculate value X
Question 02.3
📐 Calculations
You need the percentage change in mass for the 0.0 mol dm⁻³ row.
- Find the change in mass: 0.96 g
- Find the starting mass: 15.54 g
- Use the formula:
percentage change = (change ÷ starting mass) × 100 - Substitute the values:
(0.96 ÷ 15.54) × 100 - Calculate:
6.1776... - Round to 1 decimal place: 6.2%
✅ Correct answer
Value X = 6.2%
1 mark for using 0.96 ÷ 15.54 × 100
1 mark for getting about 6.17...
1 mark for rounding correctly to 6.2%
🧠 Exam technique
- Use the starting mass, not the mass after 2 hours.
- Always read the instruction about decimal places.
- Include the % symbol in your final answer.
Examiner insight: many students lost marks by dividing by the wrong mass or forgetting to round to 1 decimal place.
❌ Common errors
- Using 16.50 instead of 15.54 in the denominator.
- Doing 15.54 ÷ 0.96 the wrong way round.
- Writing 6.17% when the question asks for 1 decimal place.
- Forgetting that this is percentage change, so you must multiply by 100.
Part (d) – Complete the graph
Question 02.4
💡 Key knowledge
You had to plot the percentage change in mass against concentration of salt solution for 0.2 mol dm⁻³ to 1.0 mol dm⁻³ only, then draw a line of best fit.
✅ Correct plotting points
The four points to plot were:
| Concentration / mol dm⁻³ | Percentage change in mass / % |
|---|---|
| 0.2 | 4.1 |
| 0.4 | 2.3 |
| 0.6 | 0.5 |
| 1.0 | −2.5 |
One result at 0.8, −1.3 had already been plotted for you.
📐 How to score full marks on the graph
- Read each coordinate carefully from the table.
- Plot all 4 missing points accurately.
- Use small neat crosses.
- Draw a line of best fit through the trend of the points.
- Do not join dot-to-dot with straight lines.
2 marks for all 4 points plotted correctly
1 mark for a suitable line of best fit
The mark scheme allows a tolerance of ± ½ a small square.
1 mark can be given for 3 points plotted correctly.
🧠 Exam technique
Top answers were accurate and tidy. The best students:
- checked each x-value and y-value separately
- noticed that 0.0 mol dm⁻³ should not be plotted because the question said only 0.2 to 1.0
- drew a smooth best-fit line showing the overall downward trend
❌ Common errors
- Plotting the 0.0 mol dm⁻³ value when the question said not to.
- Using the wrong sign and plotting −1.3 or −2.5 above zero.
- Joining points dot-to-dot instead of drawing a line of best fit.
- Misreading 0.5 as 5.
Part (e) – Determine the concentration of solution Z
Question 02.5
✅ Correct answer
About 0.65 mol dm⁻³
Acceptable answers depend on the student’s own line of best fit. The mark scheme allows about 0.64 to 0.66 mol dm⁻³ if no line of best fit was drawn.
💡 Key knowledge
- The concentration of solution Z is where there is 0% change in mass.
- At this point, there is no net movement of water by osmosis.
- This means solution Z and the surrounding solution have the same concentration.
📐 How to read it from the graph
- Find 0 on the y-axis (percentage change in mass).
- Draw or imagine a horizontal line to your line of best fit.
- Drop down to the x-axis.
- Read the concentration value, which is about 0.65 mol dm⁻³.
🧠 Exam technique
This is a classic graph-interpretation question. You are not taking a value directly from a table; you are estimating from the line of best fit. Read carefully and give a sensible value from your graph.
❌ Common errors
- Choosing the nearest plotted point instead of the x-value where the line crosses 0%.
- Reading from the wrong axis.
- Giving a value with unreasonable precision, such as lots of decimal places from a graph.
Examiner insight for the whole question
💡 What separated stronger answers
- Knowing exactly what an independent variable is.
- Explaining validity in practical work clearly.
- Using the correct formula for percentage change.
- Plotting graphs accurately and drawing a sensible line of best fit.
- Understanding that the isotonic point is where percentage change = 0.
❌ Where students lost marks
- Confusing independent and dependent variables.
- Giving vague practical answers like “for accuracy” without saying why.
- Making calculation errors or not rounding correctly.
- Poor graph skills: missing points, wrong signs, or no line of best fit.
- Not using the graph properly to estimate the concentration.
🧠 Fast revision takeaway
- Osmosis = movement of water through a partially permeable membrane from dilute to more concentrated solution.
- Independent variable = what is changed.
- Percentage change = (change ÷ original) × 100.
- Line of best fit = smooth trend line, not dot-to-dot.
- 0% change in mass tells you the concentration inside solution Z.
Topics
Biology · Required Practicals · B1: Cell Biology · Biology Required Practicals
Question and mark scheme from the AQA GCSE Combined Science: Trilogy examination, Biology Paper 1 (Higher), 2024. QuestionVault is an independent revision resource; questions remain the copyright of the awarding body.