AQA GCSE Combined Science: Trilogy Biology Paper 1 (Higher), 2024: Question 4
10 marks · Standard Demand difficulty · Short Answer
Answer questions about photosynthesis, including the symbol equation, the effect of temperature on rate, enzyme denaturation at high temperature, improving experimental accuracy, greenhouse heating disadvantages, and meristem tissue in plant stem cuttings.
Practise this questionQuestion
Question text
04 This question is about photosynthesis.
04.1 What is the symbol equation for photosynthesis?
[1 mark]
Tick ( ) one box.
6CO2 + C6H12O6 ⟶ 6H2O + 6O2
C6H12O6 + 6O2 ⟶ 6CO2 + 6H2O
6CO2 + 6H2O ⟶ C6H12O6 + 6O2
6O2 + 6H2O ⟶ 6CO2 14+ C6H12O6
A student investigated the relationship between temperature and the rate
of photosynthesis.
The student measured the volume of gas produced by an aquatic plant in 1 hour.
The student collected the gas in a measuring cylinder.
Table 2 shows the results.
Table 2
Temperature Volume of gas produced
in °C in 1 hour in cm3
10 1
15 2
20 4
25 8
30 16
35 16
40 2
45 0
*0134.*2 Describe the effect of increasing temperature on the rate of photosynthesis.
Use data from Table 2.
[3 marks]
04.3 Explain why no gas was produced by the plant at 45 °C.
[2 marks]
04.4 How could the student increase the accuracy of the results?
[1 mark]
Tick ( ) one box.
Repeat the investigation, collecting the gas for 24 hours.
Repeat the investigation, measuring the volume of gas to 0.5 cm3.
Repeat the investigation using a different aquatic plant.
Repeat the investigation, using temperatures of 516 °C and 50 °C.
A person grows tomatoes in a greenhouse.
04.5 The mean temperature of the greenhouse is 15 °C.
A heater would keep the temperature of the greenhouse at 25 °C.
Suggest two reasons against using a heater set at 25 °C in the greenhouse.
Do not refer to cost in your answer.
[2 marks]
04.6 The person cut a stem from one of the tomato plants.
The cut stem was placed in soil to grow new roots.
Which tissue in the cut stem will differentiate into new root cells?
[1 mark]
Tick ( ) one box.
Epidermis
Meristem
Mesophyll
Phloem
Mark scheme
Show the mark scheme
Question 4
AO /
Question Answers Extra information Mark
Spec. Ref.
04.1 6CO2 + 6H2O ⟶ C6H12O6 + 6O2 1 AO2
4.4.1.1
AO /
Spec. Ref.
04.2 (as temperature increases) the ignore references to volume 1 AO3
rate of photosynthesis increases unqualified 4.4.1.2
then (the rate of
photosynthesis) decreases
fastest rate between 30 °C and allow a fastest rate at a 1
35 °C temperature above 30 °C and
below 35 °C
allow reaches an optimum
temperature between 30 °C and
35 °C
up to 30 °C the rate doubles allow up to 30 °C the rate 1
every 5 °C increase increases at an increasing rate
allow up to 30 °C the rate
– increases exponentially – –
AO /
Spec. Ref.
04.3 enzymes (in the plant / allow enzymes become 1 AO2
chloroplast) become destroyed denatured
do not accept enzymes are
killed AO3
do not accept cells / plants are
denatured 4.2.2.1
(because) active site changes 1 4.4.1.2
shape
OR
the active site changes shape
(1)
(so) the substrate no longer fits /
binds (1)
OR
enzymes (in the plant / allow enzymes become
chloroplast) become destroyed denatured
(1) do not accept enzymes are
killed
14 do not accept cells / plants are
denatured
(so) the substrate no longer fits /
binds (1)
ignore the plant is killed / dead
AO /
Spec. Ref.
04.4 repeat the investigation, 1 AO3
measuring the volume of gas to 4.4.1.2
0.5 cm3 – – –
AO /
Spec. Ref.
04.5 any two from: 2 AO3
• may have too many tomatoes allow the idea of a glut or all 4.4.1.2
to eat / sell ripen at the same time
• other factors could limit allow other named limiting factor
photosynthesis could limit photosynthesis
allow factors could limit tomato
production
• the optimum temperature
might be above / below 25 °C
• greenhouses are poorly 15
insulated
• pollution from the heater allow increase carbon dioxide
emissions
allow increase global warming
• increase the rate of allow increase the water loss
transpiration from the plant
• increased spread of plant
disease(s)
if no other mark awarded allow
it is a fire hazard for 1 mark
AO /
Spec. Ref.
04.6 meristem 1 AO1
4.1.2.3
Total Question 4 10
How to answer it
Photosynthesis and Temperature
What this question tests
This question tests your knowledge of the photosynthesis equation, how temperature affects the rate of photosynthesis, why high temperatures stop enzyme-controlled reactions, how to improve accuracy in an investigation, how to evaluate greenhouse growing conditions, and which plant tissue contains cells that can differentiate.
Part (a): Symbol equation for photosynthesis
✅ Correct answer
6CO₂ + 6H₂O → C₆H₁₂O₆ + 6O₂
💡 Key knowledge
- Carbon dioxide and water are the reactants.
- Glucose and oxygen are the products.
- The numbers in front must balance the atoms on both sides.
🧠 Exam technique
In a multiple-choice question, check both the substances and the balancing. A common quick check is:
- 6 carbons on each side
- 12 hydrogens on each side
- 18 oxygens on each side
❌ Common errors
- Choosing the respiration equation instead.
- Putting oxygen and glucose on the wrong side.
- Forgetting the big 6 in front of CO₂, H₂O, and O₂.
Part (b): Effect of temperature on rate of photosynthesis
Use data from Table 2
✅ Correct answer
A full-mark answer could be:
As temperature increases, the rate of photosynthesis increases and then decreases. The fastest rate is between 30 °C and 35 °C. Up to 30 °C, the rate doubles for each 5 °C increase (1, 2, 4, 8, 16 cm³).💡 Key knowledge
- The volume of gas produced in 1 hour is being used as a measure of rate.
- More gas in the same time means a faster rate of photosynthesis.
- The results rise from 10 °C to 30 °C, stay highest at 30–35 °C, then fall sharply.
🧠 Exam technique
- Use the word rate, not just “more gas”. The mark scheme says unqualified references to volume alone should be ignored.
- Quote data to support your point.
- Describe the whole pattern: increase → highest → decrease.
📐 Using the data step-by-step
- At 10 °C, gas volume is 1 cm³ in 1 hour.
- At 15 °C, it is 2 cm³ — this is double.
- At 20 °C, it is 4 cm³ — doubles again.
- At 25 °C, it is 8 cm³ — doubles again.
- At 30 °C, it is 16 cm³ — doubles again.
- At 35 °C, it stays at 16 cm³ — still the fastest rate.
- Then it drops to 2 cm³ at 40 °C and 0 cm³ at 45 °C.
❌ Common errors
- Saying the rate keeps increasing all the way to 45 °C.
- Only saying “the volume increases” without linking it to rate.
- Missing the fact that the highest rate is between 30 °C and 35 °C.
- Ignoring the decrease after 35 °C.
Part (c): Why was no gas produced at 45 °C?
✅ Correct answer
A full 2-mark answer could be:
At 45 °C, enzymes involved in photosynthesis become denatured. Their active site changes shape, so the substrate no longer fits or binds.💡 Key knowledge
- Photosynthesis is controlled by enzymes in the plant/chloroplast.
- At high temperatures, enzymes become denatured.
- When the active site changes shape, the substrate cannot bind, so the reaction stops.
🧠 Exam technique
For 2 marks, make two linked points:
- What happens to the enzyme.
- How that stops the reaction.
This is what stronger answers did in the mark scheme.
❌ Common errors
- Do not say “the enzymes are killed”. The mark scheme does not accept this.
- Do not say “the plant is denatured” or “cells are denatured”.
- “The plant died” is too vague and would not get the full explanation marks.
Part (d): How could the student increase the accuracy of the results?
✅ Correct answer
Tick:
Repeat the investigation, measuring the volume of gas to 0.5 cm³💡 Key knowledge
Accuracy means being closer to the true value. Measuring to a smaller scale division, such as 0.5 cm³, gives a more precise reading and can improve the recorded result.
🧠 Exam technique
Read the key word carefully:
- Accuracy = better measurement/detail
- Reliability = repeats and averages
Here, the best option is the one using a finer measurement scale.
❌ Common errors
- Choosing “repeat the investigation for 24 hours” — this changes the method rather than improving measurement accuracy.
- Choosing a different plant — this adds variation.
- Choosing temperatures of 5 °C and 50 °C — this gives more data points, not more accurate measurements.
Part (e): Reasons against keeping the greenhouse at 25 °C
Suggest two reasons and do not refer to cost
✅ Correct answers
Any two from the mark scheme:
- There may be too many tomatoes to eat or sell.
- Other factors could limit photosynthesis.
- The optimum temperature might be above or below 25 °C.
- Greenhouses are poorly insulated.
- Pollution from the heater.
- Increase in the rate of transpiration.
- Increased spread of plant disease(s).
💡 Key knowledge
- Temperature is only one factor affecting photosynthesis.
- Light intensity and carbon dioxide concentration may still limit the rate.
- Warmer conditions can also increase water loss by transpiration.
🧠 Exam technique
Because the question says against, give disadvantages only.
Good 2-mark responses gave two separate reasons, not one reason explained twice.
Examiner insight: top answers moved beyond “it gets hotter” and linked the temperature to biological or practical disadvantages.
❌ Common errors
- Talking about cost — the question explicitly says not to.
- Saying “25 °C increases photosynthesis” — that is a reason for, not against.
- Assuming 25 °C must be the optimum temperature.
- Giving vague answers like “it is bad for plants” without a clear biological reason.
Part (f): Tissue that differentiates into new root cells
✅ Correct answer
Meristem
💡 Key knowledge
- Meristem tissue contains unspecialised cells.
- These cells can divide and differentiate into new plant cells.
- This is why roots and shoots can grow from meristem tissue.
🧠 Exam technique
In plant tissue questions, look for the tissue linked with growth and differentiation. That is meristem.
❌ Common errors
- Epidermis protects the plant.
- Mesophyll is mainly for photosynthesis in leaves.
- Phloem transports sugars.
Quick full-mark model answers
04.2 model answer
As temperature increases, the rate of photosynthesis increases and then decreases. The fastest rate is between 30 °C and 35 °C. Up to 30 °C, the rate doubles every 5 °C increase.
04.3 model answer
At 45 °C the enzymes involved in photosynthesis are denatured. Their active site changes shape so the substrate no longer fits, so no photosynthesis happens and no gas is produced.
04.5 model answer
Other factors, such as light intensity or carbon dioxide concentration, could limit photosynthesis, so 25 °C may not increase yield much. Also, the higher temperature could increase transpiration, causing more water loss from the plants.
Final examiner tips for this question
🧠 How to score well
- Use exact scientific terms: rate, denatured, active site, meristem.
- When asked to use data, actually quote numbers or a clear pattern from the table.
- For “suggest” questions, give distinct points, not repeated versions of the same idea.
❌ Biggest traps in this question
- Mixing up photosynthesis and respiration equations.
- Describing gas volume instead of the rate of photosynthesis.
- Using incorrect enzyme language such as “enzymes are killed”.
- Ignoring command words like “against” and “do not refer to cost”.
Topics
Biology · Required Practicals · B4: Bioenergetics · B1: Cell Biology · B2: Organisation · Biology Required Practicals
Question and mark scheme from the AQA GCSE Combined Science: Trilogy examination, Biology Paper 1 (Higher), 2024. QuestionVault is an independent revision resource; questions remain the copyright of the awarding body.