AQA GCSE Combined Science: Trilogy Biology Paper 1 (Higher), 2024: Question 4

10 marks · Standard Demand difficulty · Short Answer

Answer questions about photosynthesis, including the symbol equation, the effect of temperature on rate, enzyme denaturation at high temperature, improving experimental accuracy, greenhouse heating disadvantages, and meristem tissue in plant stem cuttings.

Practise this question

Question

An exam page titled 'This question is about photosynthesis' with six parts. Part 04.1 asks for the symbol equation for photosynthesis and shows four tick-box multiple-choice equations, including the correct equation with carbon dioxide and water forming glucose and oxygen. Part 04.2 gives Table 2 with temperature in degrees Celsius and volume of gas produced in 1 hour in cubic centimetres: 10, 1; 15, 2; 20, 4; 25, 8; 30, 16; 35, 16; 40, 2; 45, 0, then asks to describe the effect of increasing temperature on the rate of photosynthesis using the data. Part 04.3 asks why no gas was produced at 45 degrees Celsius. Part 04.4 asks how to improve the accuracy of the results and gives four tick-box options. Part 04.5 describes tomatoes grown in a greenhouse at mean temperature 15 degrees Celsius and asks for two reasons against using a heater set at 25 degrees Celsius, with lined answer space. Part 04.6 says a stem from one tomato plant was cut and placed in soil to grow new roots, then asks which tissue in the cut stem will differentiate into new root cells, with tick-box options epidermis, meristem, mesophyll, and phloem.
Question text

04 This question is about photosynthesis.

04.1 What is the symbol equation for photosynthesis?

[1 mark]

Tick ( ) one box.

6CO2 + C6H12O6 ⟶ 6H2O + 6O2

C6H12O6 + 6O2 ⟶ 6CO2 + 6H2O

6CO2 + 6H2O ⟶ C6H12O6 + 6O2

6O2 + 6H2O ⟶ 6CO2 14+ C6H12O6

A student investigated the relationship between temperature and the rate

of photosynthesis.

The student measured the volume of gas produced by an aquatic plant in 1 hour.

The student collected the gas in a measuring cylinder.

Table 2 shows the results.

Table 2

Temperature Volume of gas produced

in °C in 1 hour in cm3

10 1

15 2

20 4

25 8

30 16

35 16

40 2

45 0

*0134.*2 Describe the effect of increasing temperature on the rate of photosynthesis.

Use data from Table 2.

[3 marks]

04.3 Explain why no gas was produced by the plant at 45 °C.

[2 marks]

04.4 How could the student increase the accuracy of the results?

[1 mark]

Tick ( ) one box.

Repeat the investigation, collecting the gas for 24 hours.

Repeat the investigation, measuring the volume of gas to 0.5 cm3.

Repeat the investigation using a different aquatic plant.

Repeat the investigation, using temperatures of 516 °C and 50 °C.

A person grows tomatoes in a greenhouse.

04.5 The mean temperature of the greenhouse is 15 °C.

A heater would keep the temperature of the greenhouse at 25 °C.

Suggest two reasons against using a heater set at 25 °C in the greenhouse.

Do not refer to cost in your answer.

[2 marks]

04.6 The person cut a stem from one of the tomato plants.

The cut stem was placed in soil to grow new roots.

Which tissue in the cut stem will differentiate into new root cells?

[1 mark]

Tick ( ) one box.

Epidermis

Meristem

Mesophyll

Phloem

Mark scheme

Show the mark scheme Mark scheme for AQA GCSE Combined Science: Trilogy Biology Paper 1 (Higher), 2024: Question 4

Question 4

AO /

Question Answers Extra information Mark

Spec. Ref.

04.1 6CO2 + 6H2O ⟶ C6H12O6 + 6O2 1 AO2

4.4.1.1

AO /

Spec. Ref.

04.2 (as temperature increases) the ignore references to volume 1 AO3

rate of photosynthesis increases unqualified 4.4.1.2

then (the rate of

photosynthesis) decreases

fastest rate between 30 °C and allow a fastest rate at a 1

35 °C temperature above 30 °C and

below 35 °C

allow reaches an optimum

temperature between 30 °C and

35 °C

up to 30 °C the rate doubles allow up to 30 °C the rate 1

every 5 °C increase increases at an increasing rate

allow up to 30 °C the rate

– increases exponentially – –

AO /

Spec. Ref.

04.3 enzymes (in the plant / allow enzymes become 1 AO2

chloroplast) become destroyed denatured

do not accept enzymes are

killed AO3

do not accept cells / plants are

denatured 4.2.2.1

(because) active site changes 1 4.4.1.2

shape

OR

the active site changes shape

(1)

(so) the substrate no longer fits /

binds (1)

OR

enzymes (in the plant / allow enzymes become

chloroplast) become destroyed denatured

(1) do not accept enzymes are

killed

14 do not accept cells / plants are

denatured

(so) the substrate no longer fits /

binds (1)

ignore the plant is killed / dead

AO /

Spec. Ref.

04.4 repeat the investigation, 1 AO3

measuring the volume of gas to 4.4.1.2

0.5 cm3 – – –

AO /

Spec. Ref.

04.5 any two from: 2 AO3

• may have too many tomatoes allow the idea of a glut or all 4.4.1.2

to eat / sell ripen at the same time

• other factors could limit allow other named limiting factor

photosynthesis could limit photosynthesis

allow factors could limit tomato

production

• the optimum temperature

might be above / below 25 °C

• greenhouses are poorly 15

insulated

• pollution from the heater allow increase carbon dioxide

emissions

allow increase global warming

• increase the rate of allow increase the water loss

transpiration from the plant

• increased spread of plant

disease(s)

if no other mark awarded allow

it is a fire hazard for 1 mark

AO /

Spec. Ref.

04.6 meristem 1 AO1

4.1.2.3

Total Question 4 10

How to answer it

Photosynthesis and Temperature

AQA GCSE Combined Science: Trilogy — exam-focused study guide

What this question tests

This question tests your knowledge of the photosynthesis equation, how temperature affects the rate of photosynthesis, why high temperatures stop enzyme-controlled reactions, how to improve accuracy in an investigation, how to evaluate greenhouse growing conditions, and which plant tissue contains cells that can differentiate.

Question 04.1

Part (a): Symbol equation for photosynthesis

✅ Correct answer

6CO₂ + 6H₂O → C₆H₁₂O₆ + 6O₂

1 mark: choose the correct symbol equation.

💡 Key knowledge

  • Carbon dioxide and water are the reactants.
  • Glucose and oxygen are the products.
  • The numbers in front must balance the atoms on both sides.

🧠 Exam technique

In a multiple-choice question, check both the substances and the balancing. A common quick check is:

  • 6 carbons on each side
  • 12 hydrogens on each side
  • 18 oxygens on each side

❌ Common errors

  • Choosing the respiration equation instead.
  • Putting oxygen and glucose on the wrong side.
  • Forgetting the big 6 in front of CO₂, H₂O, and O₂.
Question 04.2

Part (b): Effect of temperature on rate of photosynthesis

Use data from Table 2

✅ Correct answer

A full-mark answer could be:

As temperature increases, the rate of photosynthesis increases and then decreases. The fastest rate is between 30 °C and 35 °C. Up to 30 °C, the rate doubles for each 5 °C increase (1, 2, 4, 8, 16 cm³).
3 marks: one mark for the general trend, one for identifying the fastest rate/optimum region, and one for using the data pattern accurately.

💡 Key knowledge

  • The volume of gas produced in 1 hour is being used as a measure of rate.
  • More gas in the same time means a faster rate of photosynthesis.
  • The results rise from 10 °C to 30 °C, stay highest at 30–35 °C, then fall sharply.

🧠 Exam technique

  • Use the word rate, not just “more gas”. The mark scheme says unqualified references to volume alone should be ignored.
  • Quote data to support your point.
  • Describe the whole pattern: increase → highest → decrease.

📐 Using the data step-by-step

  1. At 10 °C, gas volume is 1 cm³ in 1 hour.
  2. At 15 °C, it is 2 cm³ — this is double.
  3. At 20 °C, it is 4 cm³ — doubles again.
  4. At 25 °C, it is 8 cm³ — doubles again.
  5. At 30 °C, it is 16 cm³ — doubles again.
  6. At 35 °C, it stays at 16 cm³ — still the fastest rate.
  7. Then it drops to 2 cm³ at 40 °C and 0 cm³ at 45 °C.

❌ Common errors

  • Saying the rate keeps increasing all the way to 45 °C.
  • Only saying “the volume increases” without linking it to rate.
  • Missing the fact that the highest rate is between 30 °C and 35 °C.
  • Ignoring the decrease after 35 °C.
Question 04.3

Part (c): Why was no gas produced at 45 °C?

✅ Correct answer

A full 2-mark answer could be:

At 45 °C, enzymes involved in photosynthesis become denatured. Their active site changes shape, so the substrate no longer fits or binds.
2 marks: one mark for enzyme destruction/denaturing, and one mark for the active site/substrate idea.

💡 Key knowledge

  • Photosynthesis is controlled by enzymes in the plant/chloroplast.
  • At high temperatures, enzymes become denatured.
  • When the active site changes shape, the substrate cannot bind, so the reaction stops.

🧠 Exam technique

For 2 marks, make two linked points:

  1. What happens to the enzyme.
  2. How that stops the reaction.

This is what stronger answers did in the mark scheme.

❌ Common errors

  • Do not say “the enzymes are killed”. The mark scheme does not accept this.
  • Do not say “the plant is denatured” or “cells are denatured”.
  • “The plant died” is too vague and would not get the full explanation marks.
Question 04.4

Part (d): How could the student increase the accuracy of the results?

✅ Correct answer

Tick:

Repeat the investigation, measuring the volume of gas to 0.5 cm³
1 mark for selecting the option that improves measurement accuracy.

💡 Key knowledge

Accuracy means being closer to the true value. Measuring to a smaller scale division, such as 0.5 cm³, gives a more precise reading and can improve the recorded result.

🧠 Exam technique

Read the key word carefully:

  • Accuracy = better measurement/detail
  • Reliability = repeats and averages

Here, the best option is the one using a finer measurement scale.

❌ Common errors

  • Choosing “repeat the investigation for 24 hours” — this changes the method rather than improving measurement accuracy.
  • Choosing a different plant — this adds variation.
  • Choosing temperatures of 5 °C and 50 °C — this gives more data points, not more accurate measurements.
Question 04.5

Part (e): Reasons against keeping the greenhouse at 25 °C

Suggest two reasons and do not refer to cost

✅ Correct answers

Any two from the mark scheme:

  • There may be too many tomatoes to eat or sell.
  • Other factors could limit photosynthesis.
  • The optimum temperature might be above or below 25 °C.
  • Greenhouses are poorly insulated.
  • Pollution from the heater.
  • Increase in the rate of transpiration.
  • Increased spread of plant disease(s).
2 marks: one mark for each valid reason.

💡 Key knowledge

  • Temperature is only one factor affecting photosynthesis.
  • Light intensity and carbon dioxide concentration may still limit the rate.
  • Warmer conditions can also increase water loss by transpiration.

🧠 Exam technique

Because the question says against, give disadvantages only.

Good 2-mark responses gave two separate reasons, not one reason explained twice.

Examiner insight: top answers moved beyond “it gets hotter” and linked the temperature to biological or practical disadvantages.

❌ Common errors

  • Talking about cost — the question explicitly says not to.
  • Saying “25 °C increases photosynthesis” — that is a reason for, not against.
  • Assuming 25 °C must be the optimum temperature.
  • Giving vague answers like “it is bad for plants” without a clear biological reason.
Question 04.6

Part (f): Tissue that differentiates into new root cells

✅ Correct answer

Meristem

1 mark for choosing meristem.

💡 Key knowledge

  • Meristem tissue contains unspecialised cells.
  • These cells can divide and differentiate into new plant cells.
  • This is why roots and shoots can grow from meristem tissue.

🧠 Exam technique

In plant tissue questions, look for the tissue linked with growth and differentiation. That is meristem.

❌ Common errors

  • Epidermis protects the plant.
  • Mesophyll is mainly for photosynthesis in leaves.
  • Phloem transports sugars.

Quick full-mark model answers

04.2 model answer

As temperature increases, the rate of photosynthesis increases and then decreases. The fastest rate is between 30 °C and 35 °C. Up to 30 °C, the rate doubles every 5 °C increase.

04.3 model answer

At 45 °C the enzymes involved in photosynthesis are denatured. Their active site changes shape so the substrate no longer fits, so no photosynthesis happens and no gas is produced.

04.5 model answer

Other factors, such as light intensity or carbon dioxide concentration, could limit photosynthesis, so 25 °C may not increase yield much. Also, the higher temperature could increase transpiration, causing more water loss from the plants.

Final examiner tips for this question

🧠 How to score well

  • Use exact scientific terms: rate, denatured, active site, meristem.
  • When asked to use data, actually quote numbers or a clear pattern from the table.
  • For “suggest” questions, give distinct points, not repeated versions of the same idea.

❌ Biggest traps in this question

  • Mixing up photosynthesis and respiration equations.
  • Describing gas volume instead of the rate of photosynthesis.
  • Using incorrect enzyme language such as “enzymes are killed”.
  • Ignoring command words like “against” and “do not refer to cost”.

Topics

Biology · Required Practicals · B4: Bioenergetics · B1: Cell Biology · B2: Organisation · Biology Required Practicals

Question and mark scheme from the AQA GCSE Combined Science: Trilogy examination, Biology Paper 1 (Higher), 2024. QuestionVault is an independent revision resource; questions remain the copyright of the awarding body.