AQA GCSE Combined Science: Trilogy Biology Paper 2 (Higher), 2024: Question 5

9 marks · Standard Demand difficulty · Short Answer

Define ecosystem, describe how animal species contribute to community stability, calculate a ratio from river pollution data, evaluate a claimed proportional relationship between bacteria and dissolved oxygen, and explain how reduced dissolved oxygen affects otter populations.

Practise this question

Question

The question page is labelled Question 05 and begins with the sentence that otters are mammals that live in river ecosystems, followed by a black-and-white photograph labelled Figure 5 showing an otter standing on grass. Part 05.1 asks for a definition of the term ecosystem for 1 mark, and part 05.2 asks for two ways animal species may be important for the stability of a whole community for 2 marks. A passage then explains that sewage was accidentally added to a river and affected the number of bacteria and the concentration of dissolved oxygen; Table 2 lists distance from where sewage was added in kilometres, number of bacteria multiplied by 1000 per cubic millimetre, and dissolved oxygen concentration in milligrams per cubic decimetre, with values from 0.0 km to 5.0 km showing bacteria increasing from 4 to 216 and dissolved oxygen decreasing from 5.4 to 2.7. Part 05.3 asks students to calculate how many times greater the number of bacteria is at 5.0 km than at the point where sewage was added, part 05.4 asks why the conclusion 'number of bacteria is proportional to concentration of dissolved oxygen' is not correct using Table 2, and part 05.5 states that otters live in water and on land and eat mainly fish, then asks how decreased dissolved oxygen in river water will affect the otter population.
Question text

05 Otters are mammals that live in river ecosystems.

Figure 5 shows an otter.

Figure 5

05.1 Define the term ‘ecosystem’.

[1 mark]

05.2 Otters are an important species for the stability of the river community.

Describe two ways animal species may be important for the stability of a

whole community.

[2 marks]

Sewage was accidentally added to a river.

The sewage moved with the river water and affected:

• the number of bacteria in the water

• the concentration of dissolved oxygen in the water.

Samples of river water were analysed at different distances from where the

sewage was added.

Table 2 shows the results.

Table 2

Distance from where Number of bacteria Concentration of dissolved

sewage was added in km × 1000/mm3 oxygen in mg/dm3

0.0 4 5.4

1.0 75 4.8

2.0 125 4.3

3.0 145 3.8

4.0 160 3.3

5.0 216 2.7

05.3 The number of bacteria at 5.0 km was greater than the number of bacteria where the

sewage was added.

Calculate how many times greater.

[1 mark]

Number of times greater21 =

05.4 A student concluded:

‘number of bacteria ∝ concentration of dissolved oxygen’

Explain why the student’s conclusion is not correct.

*20* Use Table 2.

[2 marks]

05.5 Otters:

• live in water and on the land

• eat mainly fish.

The concentration of dissolved oxygen has decreased in a river where otters live.

Explain how the decrease in the concentration of dissolved oxygen in the river water

will affect the population of otters.

[3 marks]

Mark scheme

Show the mark scheme Mark scheme for AQA GCSE Combined Science: Trilogy Biology Paper 2 (Higher), 2024: Question 5

Question 5

AO /

Question Answers Extra information Mark

Spec. Ref.

05.1 the interaction of (a community allow the interaction of (a 1 AO1

of living) organisms and (the community of) biotic parts and 4.7.1.1

non-living parts of) their the abiotic parts of their

environment / habitat environment / habitat

allow the interaction of a

community and (the non-living

parts of) their environment /

habitat

AO /

Spec. Ref.

allow named examples

05.2 throughout AO2

4.7.1.1

any two from: 2

• may be a predator therefore allow may be predator so keep 4.7.2.1

control prey population prey population (fairly) constant 4.7.3.1

• may be prey so provide ignore may be prey so provide

energy to predator food to predator

• may be a primary consumer

therefore control plant / algae

population

• may alter habitat for other

species (to survive)

• may provide shelter for other

species (to survive)

• may maintain biodiversity

• may disperse seed / pollen

AO /

Spec. Ref.

05.3 216 allow 1 AO2

( =) 54

4 216 000 4.7.3.2

( =) 54

4000

AO /

Spec. Ref.

18 ignore references to inversely

05.4 proportional

as number of bacteria allow as bacteria increases, 1 AO3

increases, the concentration of oxygen decreases 4.7.3.2

dissolved oxygen decreases

allow there is a negative

correlation

allow converse

(the relationship is) allow (the relationship is) not as 1

not number of bacteria bacteria increases, oxygen

increases and the concentration increases

of oxygen increases (in the

same ratio) allow whereas proportional

(relationship) would mean as

one increases the other

increases (in the same ratio)

allow converse

AO /

Spec. Ref.

05.5 (decrease in oxygen allow (decrease in oxygen 1 AO3

concentration causes a) concentration means) more fish

decrease in the number of fish die

(because) fish need oxygen to allow invertebrates need oxygen 1 AO2

respire (aerobically) to respire and fish eat

invertebrates

do not accept implications that

otters require oxygen from water

do not accept energy produced

/ made / created by respiration

(therefore) otter population allow rate of reproduction (in 1 AO2

decreases due to less food otter population) is lower due to

less food 4.7.2.1

allow otters migrate to another 4.7.3.2

river / area / habitat 4.4.2.1

ignore decrease in oxygen

causes decrease in otter

population unqualified

alternative answer

(decrease in oxygen

concentration means) more 19

bacteria are present (1)

(so) more fish die (from bacterial

infection) (1)

(therefore) otter population

decreases due to less food

or

rate of reproduction (in otter

population) is lower due to less

food

or

otters migrate to another river /

area / habitat

(1)

Total Question 5 9

How to answer it

Otters in River Ecosystems

What this question tests

You need to define ecosystem, explain why species can be important in a community, read data from a table, do a simple ratio calculation, spot that two variables can be linked without being directly proportional, and explain how a change in dissolved oxygen affects an animal population through the food chain.

Overall focus: ecology, data handling, and explanation

Question 05 overview

This question is mostly about clear recall and using the data correctly. Most marks come from giving the exact ecological meaning, using the table evidence, and writing cause-and-effect explanations.

Part 05.1 — Define the term “ecosystem”

✅ Correct answer

An ecosystem is the interaction of a community of living organisms and the non-living parts of their environment.

You could also say: the interaction of a community and its habitat.

💡 Key knowledge

  • Biotic = living parts.
  • Abiotic = non-living parts.
  • An ecosystem includes both, and how they interact.

🧠 Exam technique

  • Use the word interaction for full marks.
  • Don’t just write “a habitat” or “a community” on its own — that is not complete enough.

❌ Common errors

  • Only saying “a place where organisms live”.
  • Leaving out the non-living/abiotic part.
  • Not mentioning the relationship between the parts.
Mark breakdown: 1 mark for a correct definition.

Part 05.2 — Two ways animal species may be important for the stability of a whole community

✅ Correct answers

Any two of the following:

  • They may be predators, so they control the prey population.
  • They may be prey, providing energy/food to another species.
  • They may be a primary consumer, controlling plant or algae populations.
  • They may alter the habitat for other species to survive.
  • They may provide shelter for other species to survive.
  • They may maintain biodiversity.
  • They may disperse seeds or pollen.

💡 Key knowledge

  • In ecology, species can affect population size and food webs.
  • Otters are a good example of a predator that can help keep populations stable.
  • Stable communities often have balanced feeding relationships.

🧠 Exam technique

  • Write two separate points — one mark for each.
  • Develop each point slightly: say how it helps stability.
  • Named examples are allowed, but not needed.

❌ Common errors

  • Writing vague statements like “they keep the ecosystem healthy” without explaining why.
  • Repeating the same idea twice in different words.
  • Saying only “they eat other animals” without linking to stability.
Mark breakdown: 2 marks total, usually 1 mark per valid way.

Part 05.3 — Calculate how many times greater

📐 Calculation: step-by-step

Data:

  • Bacteria at 5.0 km = 216 (× 1000/mm³)
  • Bacteria where sewage was added = 4 (× 1000/mm³)
  1. Write the ratio: 216 ÷ 4
  2. Calculate: 216 ÷ 4 = 54
  3. State the answer clearly: 54 times greater

✅ Correct answer

54

So the number of bacteria at 5.0 km was 54 times greater.

🧠 Exam technique

  • Use the numbers exactly as given in the table.
  • This is a ratio question, so divide the larger number by the smaller number.
  • Because the question asks “how many times greater”, a plain number is enough.

❌ Common errors

  • Adding the numbers instead of dividing.
  • Using the wrong row from the table.
  • Forgetting to answer in words: “times greater”.
Mark breakdown: 1 mark for the correct ratio/calculation.

Part 05.4 — Explain why the student’s conclusion is not correct

✅ Correct answer

As the number of bacteria increases, the concentration of dissolved oxygen decreases.

The relationship is not proportional because the two variables do not increase in the same ratio.

💡 Key knowledge

  • The table shows a negative correlation: more bacteria, less oxygen.
  • At 0.0 km there are 4 bacteria and 5.4 mg dm⁻³ oxygen.
  • At 5.0 km there are 216 bacteria and 2.7 mg dm⁻³ oxygen.
  • These values show the numbers change in opposite directions, not in a fixed ratio.

🧠 Exam technique

  • Use the table to support your answer.
  • To show something is not proportional, say the two quantities do not increase/decrease in the same ratio.
  • A strong answer compares at least two points from the table.

❌ Common errors

  • Saying “they are inversely proportional” — this was not the wording the mark scheme wanted.
  • Writing only “bacteria increases and oxygen decreases” without saying why this disproves proportionality.
  • Confusing “correlation” with “proportional”.
Mark breakdown: 2 marks total — one for describing the trend, one for explaining why it is not proportional.

Part 05.5 — Explain how decreased dissolved oxygen affects otter population

✅ Correct answer

1. Decreased oxygen means fewer fish survive because fish need oxygen for aerobic respiration.

2. Otters then have less food because they eat mainly fish.

3. Therefore, the otter population decreases (or otters may move to another habitat).

💡 Key knowledge

  • Fish need dissolved oxygen to respire aerobically.
  • If oxygen levels fall, fish may die or become fewer in number.
  • Otters depend on fish as a food source, so fewer fish means less food for otters.
  • Less food usually leads to fewer otters surviving or reproducing.

🧠 Exam technique

  • Use a chain of reasoning: oxygen ↓ → fish ↓ → food for otters ↓ → otter population ↓.
  • There are 3 marks, so give three linked ideas.
  • You can also mention that more bacteria may be present, leading to more fish dying from infection.

❌ Common errors

  • Saying otters “need oxygen from the water” — the mark scheme does not accept that.
  • Talking only about bacteria and forgetting the fish–otter link.
  • Stopping at “fish die” without explaining the effect on otters.
  • Saying “energy from respiration decreases” — not acceptable here.
Mark breakdown: 3 marks for a linked explanation: oxygen affects fish, fish affect otters, so otter numbers fall.

What top answers did well

💡 Top-level features

  • Used precise biology language: biotic, abiotic, predator, prey, proportional.
  • Read the table carefully and used the data in explanations.
  • Wrote cause-and-effect chains rather than isolated facts.
  • Gave just enough detail for the mark scheme, without overcomplicating it.

🧠 Final exam tips

  • For definition questions, learn the exact wording.
  • For “explain” questions, always link the steps together.
  • For data questions, quote numbers from the table.
  • Check whether the question wants a trend, a calculation, or a reason.

Topics

Biology · B7: Ecology

Question and mark scheme from the AQA GCSE Combined Science: Trilogy examination, Biology Paper 2 (Higher), 2024. QuestionVault is an independent revision resource; questions remain the copyright of the awarding body.