AQA GCSE Biology Biology Paper 2 (Foundation), June 2023: Question 4

11 marks · Low Demand difficulty · Short Answer

Evaluate energy efficiency in indoor versus outdoor cattle farming and analyze antibiotic resistance development and spread in livestock.

Practise this question

Question

Question 4 begins with photographs comparing cows kept indoors in a shed versus cows kept outdoors in a field, alongside Table 3 showing energy inputs and outputs in kJ/m²/year for indoors and outdoors. Subquestions 04.1 to 04.4 ask for calculation of total energy input outdoors, percentage efficiency indoors, reasons for higher efficiency indoors (multiple choice checkboxes), and why diseases spread faster indoors. Subquestion 04.5 introduces Figure 7, a diagram of a Petri dish with antibiotic discs A to H and bacterial inhibition zones, asking to identify an antibiotic the bacteria are resistant to. Subquestions 04.6 to 04.8 ask candidates to complete sentences about DNA mutations and bacterial reproduction rate, and suggest why antibiotic resistance is problematic for farmers.
Question text

04 Some farmers keep cows indoors in large sheds.

Other farmers keep cows outdoors in fields of grass.

Figure 6 shows cows being kept indoors and outdoors.

Figure 6

Table 3 shows the energy inputs and energy outputs for keeping cows.

Table 3

Energy in kJ/m2/year

Indoors Outdoors

Input as food 10 000 5 950

Input as fossil fuel 6 000 50

Output as meat and milk 40 2

04.1 Calculate the total energy input for keeping cows outdoors.

Use data from Table 3.

[1 mark]

19 2

Total energy input = kJ/m /year

04.2 The total energy input for keeping cows indoors is 16 000 kJ/m2/year.

*18* Calculate the percentage efficiency of keeping cows indoors.

Use the equation:

energy output

percentage efficiency = × 100

total energy input

[2 marks]

Percentage efficiency = %

04.3 The percentage efficiency of keeping cows outdoors is 0.03%.

Why is it more energy efficient to keep cows indoors than to keep cows outdoors?

[2 marks]

Tick ( ) two boxes.

Cows are more stressed indoors.

Cows move less indoors.

It is noisier indoors.

It is warmer indoors.

There is less light indoors.

Diseases in cows can cause problems for farmers.

04.4 Suggest why diseases spread more quickly when the cows are kept indoors.

[1 mark]

One species of bacterium causes a disease in cows.

Scientists investigated the effect of eight different antibiotics on the growth of this

*19* species of bacterium.

The scientists put discs containing the different antibiotics onto a Petri dish containing

the bacteria.

Antibiotics A to H were used in the investigation.

Figure 7 shows what the Petri dish looked like after 2 days.

Figure 7

04.5 This species of bacterium is resistant to some of the antibiotics.

Give the letter of one antibiotic the bacterium is resistant to.

[1 mark]

04.6 Complete the sentence.

Choose the answer from the box.

[1 mark]

*20* carbohydrate DNA lipid

Antibiotic resistance in a single bacterium is caused by a change in the

bacterium’s .

04.7 Complete the sentence.

Choose the answer from the box.

[1 mark]

excretion feeding reproduction

A change in one bacterium can cause millions of bacteria to become resistant to

the antibiotic.

This is because bacteria have a high rate of .

04.8 Suggest why the production of millions of antibiotic-resistant bacteria is a problem

for farmers.

[2 marks]

Mark scheme

Show the mark scheme Mark scheme for Question 4 provides answers for each part: 04.1 requires 6000 kJ/m²/year (1 mark); 04.2 allows 1 mark for the working (40 / 16 000) × 100 and 1 mark for 0.25% (2 marks total); 04.3 awards 1 mark each for ticking 'cows move less indoors' and 'it is warmer indoors' (2 marks); 04.4 gives 1 mark for cows being closer together; 04.5 gives 1 mark for any one of D, G, or H; 04.6 gives 1 mark for DNA; 04.7 gives 1 mark for reproduction; and 04.8 awards 2 marks for points relating to animal illness/death, disease spread, lack of cure, reduced productivity, or financial loss.

Question 4

AO /

Question Answers Extra information Mark

Spec Ref.

04.1 6000 (kJ/m2/year) 1 AO2

4.7.5.2

AO /

Spec Ref.

04.2 40 1 AO2

× 100 4.7.5.2

16 000

0.25 (%) 1

AO /

Spec Ref.

04.3 cows move less indoors 1 AO1

4.7.5.2

it is warmer indoors

AO /

Spec Ref.

04.4 cows are close(r) together allow cows are in an enclosed 1 AO2

space 4.7.5.2

4.3.1.1

AO /

Spec Ref.

04.5 any one from: 1 AO3

• D 4.6.3.7

4.1.1.6

• G

4.3.1.8

• H

AO /

Spec Ref.

04.6 DNA 1 AO1

4.6.3.7

AO /

Spec Ref.

04.7 reproduction 1 AO2

4.6.3.7

4.3.1.1

AO / 13

Spec Ref.

ignore reference to crops AO2

04.8 any two from: 2

4.3.1.1

• many animals will become ill allow many animals may die 4.6.3.7

4.7.5.2

• resistant bacteria passed from allow disease passed from

animal to animal animal to animal or other

animals infected

• animals cannot be cured (by allow antibiotic will not work

the antibiotic)

• animals may be less allow less meat / milk produced

productive / efficient allow less growth

• farmer may lose profit / allow cannot sell infected

money produce / animals

Total Question 4 11

How to answer it

Farming Efficiency & Antibiotic Resistance in Cattle

What This Question Tests

This question assesses knowledge from Ecology (Food Production) and Infection and Response (Antibiotics & Genetics):

  • Energy transfers in agriculture: Calculating total energy inputs and percentage efficiency from data tables.
  • Intensive vs. free-range farming: Explaining why restricting movement and maintaining temperature conserves biomass and energy.
  • Pathogen transmission: Understanding how high-density indoor housing affects the spread of infectious disease.
  • Interpreting agar plates (clear zones): Identifying antibiotic resistance from inhibition zones.
  • Bacterial genetics & evolution: Recalling that mutations occur in DNA, rapid bacterial reproduction multiplies resistant strains, and explaining the economic and veterinary consequences for farmers.
Question 04.1 • 1 Mark

Total Energy Input Calculation (Outdoors)

Extracting and summing energy inputs from tabular data

📐 Step-by-Step Calculation

  1. Locate the Outdoors column in Table 3.
  2. Identify all input rows:
    • Input as food = 5 950 kJ/m²/year
    • Input as fossil fuel = 50 kJ/m²/year
  3. Sum both inputs:
    5 950 + 50 = 6 000

✅ Correct Answer

6 000 (kJ/m²/year)

1 Mark: Correct value only.

❌ Common Errors

  • Including the output: Adding the output value (2 kJ/m²/year) to get 6 002. Read the prompt carefully—it asks only for energy input.
  • Using the wrong column: Reading values from the "Indoors" column instead of "Outdoors".
Question 04.2 • 2 Marks

Percentage Efficiency Calculation (Indoors)

Using the standard efficiency formula with indoor cow data

📐 Step-by-Step Calculation

  1. Identify the formula:
    Percentage efficiency = (Energy output ÷ Total energy input) × 100
  2. Substitute values for Indoors:
    • Energy output (meat and milk) = 40
    • Total energy input = 16 000 (given in the question prompt)
  3. Calculate:
    (40 ÷ 16 000) × 100 = 0.0025 × 100 = 0.25%

✅ Correct Answer & Mark Scheme

  • Step 1: Correct substitution: (40 ÷ 16 000) × 100 [1 mark]
  • Step 2: Final answer: 0.25 (%) [1 mark]
An answer of 0.25 scores 2 marks directly.

🧠 Exam Technique: Use Given Values

The question conveniently gave you the total energy input ( 16 000 kJ/m²/year ). Always check if the question provides totals before spending time recalculating them yourself.

❌ Common Errors

  • Forgetting to multiply by 100 (giving 0.0025).
  • Inverting the division: 16 000 ÷ 40 = 400. Remember efficiency can never exceed 100%!
Question 04.3 • 2 Marks

Why Indoor Farming is More Energy Efficient

Biological principles of intensive livestock farming (Tick two boxes)

✅ Correct Selections

  • ☑ Cows move less indoors. [1 mark]
  • ☑ It is warmer indoors. [1 mark]
Tick exactly two boxes. If three boxes are ticked, marks will be deducted.

💡 Key Knowledge: Biomass Conservation

Intensive farming maximises efficiency by reducing energy losses at each trophic level:

  • Reduced movement: Less muscle contraction means less aerobic respiration, saving glucose.
  • Warmer environment: Less heat energy lost maintaining body temperature (thermoregulation).
  • More remaining energy is converted into biomass (growth/meat and milk).
Question 04.4 • 1 Mark

Spread of Disease Indoors

Linking housing density to pathogen transmission

✅ Correct Answer

Any one point:

  • Cows are close(r) together.
  • Cows are in an enclosed space.
1 Mark: Clear reference to physical proximity or shared enclosed air/space.

🧠 Exam Technique

Use comparative words where relevant (e.g. "closer together" rather than just "they are together"). Avoid vague answers like "it is dirty" or "germs like warmth" unless supported by clear biological mechanism.

Question 04.5 • 1 Mark

Identifying Antibiotic Resistance

Interpreting agar plates and zones of inhibition

✅ Correct Answer

Any one letter from:

  • D
  • G
  • H
1 Mark: Only one letter required.

💡 Key Knowledge: Clear Zones (Inhibition Zones)

  • Clear area (no bacterial lawn): Bacteria were killed or inhibited → bacteria are susceptible/sensitive to this antibiotic.
  • No clear area (lawn grows right up to disc): Bacteria were unaffected and continued to grow → bacteria are resistant to this antibiotic.
  • Discs D, G, and H have no clear rings around them.

❌ Common Errors

Choosing C because it has the largest clear zone. Disc C is the most effective antibiotic against the bacteria, not the one the bacteria are resistant to!

Questions 04.6 & 04.7 • 2 Marks Total

Causes and Spread of Resistance

Genetics and binary fission in bacteria

04.6 • 1 Mark

Sentence Completion: Cause of Resistance

"Antibiotic resistance in a single bacterium is caused by a change in the bacterium's ________."

✅ Correct word: DNA

💡 Explanation: A random mutation alters the bacterial DNA, producing a new gene that provides resistance (e.g. producing an enzyme that breaks down the antibiotic).

04.7 • 1 Mark

Sentence Completion: Spread of Resistance

"This is because bacteria have a high rate of ________."

✅ Correct word: reproduction

💡 Explanation: Bacteria divide very quickly by binary fission (often every 20 minutes under ideal conditions), rapidly passing the mutated resistance gene to offspring.

Question 04.8 • 2 Marks

Consequences of Resistant Bacteria for Farmers

Evaluating veterinary and financial impacts

✅ Mark Scheme: Any TWO points

  • Many animals will become ill / many animals may die.
  • Resistant bacteria / disease can be passed from animal to animal (or other animals infected).
  • Animals cannot be cured (by that antibiotic) / the antibiotic will no longer work.
  • Animals become less productive / efficient (less meat or milk produced; reduced growth).
  • Farmer loses profit / money (due to lost stock, reduced yield, or unmarketable produce).
2 Marks: 1 mark per distinct valid reason.

❌ Examiner Guidance & Exclusions

  • Ignore references to crops: This context is strictly dairy/beef farming.
  • Avoid generic single words: Writing just "death" or "money" is too vague. State clearly: "cows may die" or "farmer loses money through reduced milk yield".
  • Human health: While resistant bacteria can spread to humans, the question specifically asks why it is a problem for farmers.

Topics

Biology · B3: Infection and Response · B6: Inheritance, Variation and Evolution · B7: Ecology

Question and mark scheme from the AQA GCSE Biology examination, Biology Paper 2 (Foundation), June 2023. QuestionVault is an independent revision resource; questions remain the copyright of the awarding body.