AQA GCSE Biology Biology Paper 1 (Higher), 2024: Question 4

12 marks · Standard Demand difficulty · Short Answer

Identify and explain the structures and functions of plant tissues involved in transport and differentiation, including xylem, phloem and specialised leaf tissues.

Practise this question

Question

A full exam page for Question 4 on plant tissues, divided into parts 04.1 to 04.6 with a total of 12 marks. Part 04.1 asks students to complete three sentences naming the leaf tissue with most chloroplasts, the leaf tissue with many air spaces, and the plant tissue that can differentiate throughout life; parts 04.2 and 04.3 ask for the strengthening substance in xylem and the name of sugar transport in phloem. A missing Figure 3 is described as a photograph of two cells from phloem tissue, and later parts ask students to name part Y in the figure, explain how sub-cellular structures move dissolved sugars from cell X into the phloem cell when the phloem has a higher sugar concentration, and describe one structural change when an unspecialised cell differentiates into a phloem cell using Figure 3.
Question text

04 Plants contain many different tissues.

04.1 Complete the sentences.

[3 marks]

The leaf tissue that contains the most chloroplasts is

the .

The leaf tissue that contains many air spaces is

the .

The plant tissue that can differentiate throughout the life of the plant is

the .

04.2 Xylem tissue transports water through a plant.

The walls of xylem cells contain cellulose.

Name one other substance that strengthens xylem tissue.

[1 mark]

04.3 Phloem tissue transports dissolved sugars around a plant.

Name the process that transports dissolved sugars around a plant.

[1 mark]

Figure 3 shows two plant cells.

Figure 3

Figure 3 cannot be reproduced here due to third-party copyright restrictions.

It is a photograph showing two cells from phloem tissue from page numbers 111-120 of the

following publication:

Cytochemical Localization of Adenosine Triphosphatase in the Phloem of Pisum sativum

and its Relation to the Function of Transfer Cells, Planta Vol. 2 by B J Bentwood and J

Cronshaw

04.4 Name part Y in Figure 3.

[1 mark]

04.5 The phloem tissue transports sugars to other parts of the plant.

The concentration of dissolved sugars in the phloem cell in Figure 3 is higher than

in cell X.

Explain how sub-cellular structures help to move dissolved sugars from cell X into the

phloem cell.

[5 marks]

04.6 New phloem cells form when unspecialised plant cells differentiate and

become specialised.

Describe one change in structure that occurs when an unspecialised cell differentiates

to form a phloem cell.

Use Figure 3.

[1 mark]

Mark scheme

Show the mark scheme The mark scheme is a table for Question 4 showing accepted answers, extra information and mark allocations for each sub-question. It awards: palisade mesophyll, spongy mesophyll and meristem for 04.1; lignin for 04.2; translocation for 04.3; vacuole for 04.4; and for 04.5 credit for mitochondria, aerobic respiration, release of energy, moving sugars against the concentration gradient, and active transport. For 04.6 it accepts one structural change such as loss of cytoplasm, nucleus, mitochondria, ribosomes or other sub-cellular structures, or perforated end walls forming sieve plates; total marks for Question 4 are 12.

Question 4

AO /

Question Answers Extra information Mark

Spec Ref.

04.1 must be in this order AO1

4.1.2.3

palisade (mesophyll / layer / 1 4.2.3.1

cells)

do not accept reference to

spongy mesophyll / layer a single cell, once only 1

meristem 1

AO /

Spec Ref.

04.2 lignin ignore cellulose 1 AO1

4.1.1.3

4.2.3.1

4.2.3.2

AO /

Spec Ref.

04.3 translocation ignore active transport 1 AO1

4.2.3.2

AO /

Spec Ref.

04.4 (permanent) vacuole 1 AO3

4.1.1.2

AO /

Spec Ref.

04.5 (cell X contains) mitochondria 1 AO3

for (aerobic) respiration do not accept anaerobic 1 AO2

respiration

(mitochondria / respiration) do not accept energy produced 1 AO2

releases energy / made / created

energy needed to move allow energy needed to move 1 AO2

(dissolved) sugar(s) against / (dissolved) sugar(s) from a low

up the concentration gradient concentration to a high

concentration

by active transport 1 AO2 17

4.1.3.3

4.1.1.2

4.4.2.1

AO /

Spec Ref.

04.6 ignore description of a phloem AO2

cell 4.1.2.3

any one from: 1 4.1.1.4

• loss of cytoplasm 4.2.3.2

• loss of nucleus

• loss of mitochondria

• loss of ribosomes

• loss of sub-cellular structures

• end walls become perforated allow reference to sieve plate

formation

allow cell walls form

allow (larger) vacuole forms

ignore reference to change in

size / shape

Total Question 4 12

How to answer it

Plant Tissues and Transport

What this question tests
Recall of key plant tissues and their functions, plus basic explanations of how substances move in plants. You need to know: palisade mesophyll, spongy mesophyll, meristem, lignin, translocation, phloem structure, and how mitochondria provide energy for active transport.
Question 4 overview

This is a short-answer GCSE Biology question with simple recall parts and one longer explanation. Most marks come from using the correct technical term and giving clear, linked biology points.

Part 4.1 — Complete the sentences

3 marks: naming plant tissues

✅ Correct answers

  • The leaf tissue that contains the most chloroplasts is the palisade mesophyll.
  • The leaf tissue that contains many air spaces is the spongy mesophyll.
  • The plant tissue that can differentiate throughout the life of the plant is the meristem.

💡 Key knowledge

  • Palisade mesophyll is packed with chloroplasts for photosynthesis.
  • Spongy mesophyll has air spaces to allow gases to move quickly through the leaf.
  • Meristem contains unspecialised cells that can divide and become different cell types.

🧠 Exam technique

  • These are one-word / one-phrase recall marks.
  • Write the exact tissue name in the right order.
  • Do not describe the tissue unless asked — the mark is for the correct term.

❌ Common errors

  • Writing just chloroplast instead of palisade mesophyll.
  • Writing spongy cell or one cell only — the mark scheme wants a tissue, not a single cell.
  • Confusing meristem with mature tissues that are already specialised.
How marks are awarded: 1 mark for each correct tissue. Answers must be in the correct order shown in the sentence.

Part 4.2 — Xylem strengthening

1 mark: one substance that strengthens xylem

✅ Correct answer

Lignin

💡 Key knowledge

Lignin is deposited in xylem walls and makes them thick, strong, and waterproof. This helps xylem vessels transport water and resist collapse.

🧠 Exam technique

If the question asks for one other substance, give just the named substance. The mark scheme accepts lignin.

❌ Common errors

  • Writing cellulose — this was already given in the question.
  • Writing vague phrases like “strong wall material”.

Part 4.3 — Moving sugars in phloem

1 mark: name the process

✅ Correct answer

Translocation

💡 Key knowledge

Translocation is the movement of dissolved sugars in the phloem around the plant.

🧠 Exam technique

Use the plant-transport word translocation, not the general term “transport”.

❌ Common errors

  • Answering active transport — the mark scheme says ignore this.
  • Writing transpiration — that is water movement in xylem, not sugar movement in phloem.

Part 4.4 — Identify part Y in Figure 3

1 mark: structure in a phloem cell

✅ Correct answer

(Permanent) vacuole

💡 Key knowledge

Plant cells often contain a large permanent vacuole, which helps maintain turgor and can be seen in cell diagrams or micrographs.

🧠 Exam technique

When a figure is used, match the label to a visible sub-cellular structure. Here, the mark scheme accepts vacuole or permanent vacuole.

❌ Common errors

  • Writing cell membrane or cell wall without checking the label.
  • Giving a description instead of the structure name.

Part 4.5 — Explain how sub-cellular structures help move sugars

5 marks: explanation of active transport into phloem

✅ Correct answer

  • Cell X contains mitochondria.
  • Mitochondria carry out aerobic respiration.
  • Respiration releases energy.
  • This energy is needed to move dissolved sugars against the concentration gradient from a low concentration to a high concentration.
  • The sugars are moved by active transport.

💡 Key knowledge

  • Active transport uses energy from respiration.
  • It moves substances from low concentration to high concentration.
  • Mitochondria are the site of aerobic respiration, so cells with lots of active transport need many mitochondria.

🧠 Exam technique

  • To get full marks, link the ideas in a chain: mitochondria → respiration → energy → active transport → against concentration gradient.
  • Each linked idea earns a mark when clearly stated.
  • Use the phrase against the concentration gradient for top credit.

❌ Common errors

  • Writing anaerobic respiration — this is not accepted.
  • Saying “mitochondria make energy” — the mark scheme rewards releases energy, not “make/produce”.
  • Forgetting to mention the concentration gradient.
  • Talking about diffusion alone — this question is about active transport because sugar moves from low to high concentration.

📐 Step-by-step answer structure

  1. Cell X contains mitochondria.
  2. Mitochondria carry out aerobic respiration.
  3. Respiration releases energy.
  4. This energy is used to move dissolved sugars.
  5. The sugars are moved against the concentration gradient by active transport.
Why some students lost marks: The examiner reward came from linking structure to function. Students often named mitochondria but did not explain how they help sugar movement. A strong answer clearly explains that energy from respiration powers active transport.

Part 4.6 — Change in structure during differentiation

1 mark: one structural change in a phloem cell

✅ Correct answers

  • Loss of cytoplasm
  • Loss of nucleus
  • Loss of mitochondria
  • Loss of ribosomes
  • Loss of sub-cellular structures
  • End walls become perforated

💡 Key knowledge

When a plant cell differentiates into a phloem cell, it becomes specialised for transport. Some internal structures are lost, and sieve plates form to help sugars move through the phloem.

🧠 Exam technique

Only one correct structural change is needed. Give a clear feature seen in mature phloem cells, such as loss of nucleus or perforated end walls.

❌ Common errors

  • Describing a phloem cell in general without stating a change.
  • Writing “bigger” or “different shape” — the mark scheme does not accept size/shape changes here.
  • Giving xylem features instead of phloem features.
Examiner insight: Top responses named a precise change, such as loss of nucleus or end walls become perforated. Weak answers used vague statements like “it changes” or “becomes specialised” without a structural detail.

Quick revision summary

✅ Must-know terms

  • Palisade mesophyll
  • Spongy mesophyll
  • Meristem
  • Lignin
  • Translocation
  • Active transport
  • Mitochondria
  • Permanent vacuole

🧠 How to get full marks

  • Use exact biology terms.
  • For explanations, link structure to function.
  • For transport questions, mention energy and concentration gradients.
  • Keep answers concise and focused on the mark scheme.

Topics

Biology · B1: Cell Biology · B2: Organisation · B4: Bioenergetics

Question and mark scheme from the AQA GCSE Biology examination, Biology Paper 1 (Higher), 2024. QuestionVault is an independent revision resource; questions remain the copyright of the awarding body.