AQA GCSE Biology Biology Paper 1 (Higher), June 2025

Every question from AQA GCSE Biology Biology Paper 1 (Higher), June 2025 (8461): 8 questions, 100 marks, each with its mark scheme and topic.

Original question paper

  1. Question 1 11 marks

    Answer questions about measles transmission, symptoms, plant origins of aspirin, herd immunity, and calculate values and interpret data from a bar chart comparing measles severity in vaccinated versus unvaccinated children.

    Biology · B3: Infection and Response

  2. Question 2 14 marks

    Answer questions on obesity as a risk factor, plot a graph of sugar intake versus obesity, interpret scatter graphs, and explain enzyme activity with temperature and pH.

    Biology · Required Practicals · B2: Organisation · Required Practicals

  3. Question 3 8 marks

    Evaluate an experimental method investigating osmosis in potato tissue to improve validity, explain mass increase in distilled water, and compare active transport with osmosis.

    Biology · Required Practicals · B1: Cell Biology · Required Practicals

  4. Question 4 16 marks

    Investigate the effect of temperature on the rate of photosynthesis in pondweed, perform rate calculations with units, and explain plant diseases and mineral deficiencies.

    Biology · Required Practicals · B3: Infection and Response · B4: Bioenergetics · Required Practicals

  5. Question 5 16 marks

    Answer questions on the human circulatory system, magnification calculations of red blood cells, blood vessels and heart anatomy, and cellular respiration during exercise.

    Biology · B1: Cell Biology · B2: Organisation · B4: Bioenergetics

  6. Question 6 9 marks

    Define diffusion, explain gill adaptations for gas exchange in fish, and explain why metabolic rate increases during swimming.

    Biology · B1: Cell Biology · B4: Bioenergetics

  7. Question 7 17 marks

    Explain treatments for severe liver disease including stem cells, monoclonal antibody production to prevent rejection, and the role of bile in fat digestion.

    Biology · B1: Cell Biology · B2: Organisation · B3: Infection and Response

  8. Question 8 9 marks

    Answer questions comparing bacterial and yeast cell structures, comparing antibiotic effectiveness from a growth curve graph, and estimating bacterial population density.

    Biology · B1: Cell Biology · B3: Infection and Response

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