AQA GCSE Biology Biology Paper 1 (Higher), June 2025: Question 6

9 marks · Standard Demand difficulty · Short Answer

Define diffusion, explain gill adaptations for gas exchange in fish, and explain why metabolic rate increases during swimming.

Practise this question

Question

Question 6 consists of three parts. Part 06.1 asks to define the term 'diffusion' for 1 mark. Part 06.2 presents Figure 8 showing a fish with a magnified diagram of its gills, labeling a blood vessel, gill filament, direction of water flow, and blood flow; it asks to explain how gills are adapted to maximise the exchange of oxygen between the water and the blood for 4 marks. Part 06.3 states that some fish rest on the sea floor and asks to explain why the metabolic rate of a fish is higher when swimming compared with when resting for 4 marks.
Question text

06 Diffusion is important for gas exchange.

06.1 Define the term ‘diffusion’.

[1 mark]

06.2 Fish exchange gases through gills.

Oxygen from the water enters the blood of a fish through the gills.

Figure 8 shows part of a gill.

Figure 8

Explain how gills are adapted to maximise the exchange of oxygen between the water

and the blood.

[4 marks]

06.3 Some fish rest on the sea floor.

*26* Explain why the metabolic rate of a fish is higher when swimming compared with

when resting.

[4 marks]

Mark scheme

Show the mark scheme Mark scheme for Question 6: 06.1 awards 1 mark for movement or spreading of particles from an area of higher concentration to lower concentration. 06.2 awards 4 marks for adaptations: large surface area for diffusion, good blood supply/capillary network to maintain concentration gradient, thin walls of filaments/capillaries for short diffusion distance, and water in close proximity to blood supply for short diffusion distance. 06.3 awards 4 marks for linking increased movement to requiring more energy, needing more respiration, for increased muscle contraction.

Question 6

AO /

Question Answers Extra information Mark

Spec Ref.

06.1 movement / spreading of allow movement / spreading of 1 AO1

particles from (an area of) substances / chemicals / gases / 4.1.3.1

high(er) concentration to low(er) liquids from (an area of) high(er)

concentration concentration to low(er)

concentration

allow movement / spreading of

substances / chemicals / gases /

liquids down a concentration

gradient

ignore reference to membrane

do not accept reference to

against the concentration

gradient

AO /

Spec Ref.

06.2 allow blood vessels for AO1

capillaries 4.1.3.1

do not accept arteries / veins for

capillaries

(gills / filaments) provide a large 1

surface area for (maximum)

diffusion / absorption

good blood supply to maintain 1

concentration gradient

or

has a capillary network to

maintain concentration gradient

filaments / capillaries have thin ignore membranes / filaments / 1

walls for a short diffusion capillaries are one cell thick

distance / time

or do not accept reference to cell

filaments / capillaries have walls walls

that are one cell thick for a short

diffusion distance / time

water in close proximity to blood allow blood supply is close to gill 1

supply for a short diffusion surface for a short diffusion

distance / time distance / time

allow a counter-current flow to

maintain a concentration

gradient

AO /

Question Answers Extra information Mark 23

Spec Ref.

06.3 allow converse if clearly AO2

describing resting fish 4.4.2.3

4.4.2.1

(increased) movement ignore swimming 1

(which) needs (more) energy do not accept energy produced 1

or / made / created

(more) energy provided (by do not accept energy for

respiration) respiration

(so) more respiration needs to 1

take place

(for increased) muscle 1

contraction

Total Question 6 9

How to answer it

Gas Exchange in Fish & Metabolic Rate

📋 What This Question Tests

This question assesses your understanding of fundamental transport processes and bioenergetics:

  • Recall: The exact scientific definition of diffusion (Specification 4.1.3.1).
  • Application & Explanation: How specialised exchange surfaces (fish gills) are adapted to maximise the rate of diffusion (Specification 4.1.3.1).
  • Linking Concepts: Explaining changes in metabolic rate during physical activity by linking muscle contraction, energy demand, and cellular respiration (Specification 4.4.2.1 & 4.4.2.3).
Question 06.1 · 1 Mark

Define the term 'diffusion'

AO1 Recall · Transport in Cells

✅ Acceptable Answers (1 Mark)

Any precise definition stating movement down a concentration gradient:

  • The movement (or spreading out) of particles from an area of higher concentration to an area of lower concentration.
  • Spreading of particles / substances down a concentration gradient.

💡 Key Knowledge

Diffusion is a passive process driven by the random motion of particles in liquids or gases. It requires no energy input from respiration.

❌ Common Errors

  • Saying "against the concentration gradient" (this describes active transport and loses the mark!).
  • Forgetting to mention where they move from and to (e.g., just writing "particles spreading out").

🧠 Exam Technique

Learn this definition verbatim! You don't need to mention membranes here, but if you do, do not confuse it with osmosis (osmosis is water only across a partially permeable membrane).

Mark Scheme Guidance: Allow movement/spreading of particles/substances/chemicals/gases/liquids. Do not accept reference to "against the concentration gradient".
Question 06.2 · 4 Marks

Gills Adaptations for Oxygen Exchange

AO1 Demonstration of Knowledge · Gas Exchange in Organisms

✅ Full Mark Breakdown (Any 4 of the following):

  • Mark 1 (Surface Area): (Many gill filaments / gills) provide a large surface area for (maximum) diffusion.
  • Mark 2 (Concentration Gradient): Has a good blood supply / dense capillary network to maintain a steep concentration gradient.
  • Mark 3 (Diffusion Distance): Filaments / capillaries have thin walls (or are one cell thick) for a short diffusion distance / time.
  • Mark 4 (Proximity / Flow): Water is in close proximity to the blood supply (short diffusion distance) OR counter-current flow of water and blood to maintain concentration gradient.

🧠 The "Feature + Reason" Rule

Notice how every adaptation pairs a structural feature with its functional purpose:

  • Large surface area ➔ increases rate of diffusion.
  • Thin walls (one cell thick) ➔ short diffusion pathway.
  • Rich capillary network ➔ maintains steep concentration gradient.

Always write both halves to guarantee the marks!

💡 Fick's Law of Diffusion

Rate of Diffusion ∝ (Surface Area × Concentration Difference) ÷ Thickness of Membrane.

Gills maximise diffusion by making Surface Area large, Concentration Difference large (via blood and water movement), and Thickness minimal.

❌ Common Misconceptions

  • "Cell walls": Animal cells DO NOT have cell walls! Saying "the filaments have thin cell walls" is rejected. Say "thin walls that are one cell thick".
  • Vague vessels: Do not use "arteries" or "veins" when explaining exchange surfaces—oxygen enters via capillaries or blood vessels.
  • Writing only the feature without the mechanism (e.g. just writing "large surface area" with no reference to why).
Mark Scheme Guidance: Allow "blood vessels" for capillaries. Do not accept arteries/veins for capillaries. Ignore "membranes/filaments are one cell thick" unless specifying walls are one cell thick.
Question 06.3 · 4 Marks

Metabolic Rate During Swimming vs. Resting

AO2 Application of Knowledge · Respiration & Muscle Contraction

✅ Complete 4-Step Chain of Explanation:

  1. Point 1: Swimming involves increased movement. [1 mark]
  2. Point 2: This requires increased / more muscle contraction. [1 mark]
  3. Point 3: Which requires more energy (provided by respiration). [1 mark]
  4. Point 4: Therefore, more respiration must take place (increasing overall metabolic rate). [1 mark]

🧠 Building an Unbroken Logic Chain

Examiners love cause-and-effect chains for exercise questions. Link them step-by-step:

Movement ➔ Muscle Contraction ➔ Needs Energy ➔ More Respiration

If you miss any link in the chain, you miss a mark.

❌ Major Examiner Traps

  • "Energy is produced / created / made": According to the law of conservation of energy, energy cannot be created. Respiration transfers or releases energy. Writing "energy is produced" loses the mark!
  • "Energy for respiration": Respiration does not need energy—it supplies energy for muscle contraction.
  • Forgetting to explicitly mention muscle contraction. Swimming is just the observable action; the physiological cause is contracting muscles.

💡 What is Metabolic Rate?

Metabolic rate is the rate at which all the chemical reactions in the body are carried out. Since respiration is a continuous cellular chemical reaction, an increase in respiration directly increases the metabolic rate.

Mark Scheme Guidance: Allow the converse if clearly describing a resting fish (e.g. less movement = less contraction = less energy needed = less respiration). Do not accept "energy produced / made / created".

Topics

Biology · B1: Cell Biology · B4: Bioenergetics

Question and mark scheme from the AQA GCSE Biology examination, Biology Paper 1 (Higher), June 2025. QuestionVault is an independent revision resource; questions remain the copyright of the awarding body.