AQA GCSE Combined Science: Trilogy Biology Paper 1 (Higher), 2018: Question 3

14 marks · Standard Demand difficulty · Extended Answer

Answer a series of questions about photosynthesis, including the balanced equation, reaction type, improving a pondweed practical, explaining temperature control, using the inverse square law, predicting bubble counts, and modifying the method to test carbon dioxide concentration.

Practise this question

Question

The question page is a multi-part biology exam item about photosynthesis worth 14 marks in total. It includes two multiple-choice questions asking for the balanced symbol equation for photosynthesis and the type of reaction, followed by a diagram labelled Figure 5 showing a test tube of pondweed in 0.2% sodium hydrogencarbonate solution placed at measured distances from an LED light source along a horizontal scale in centimetres. The method states that the student places pondweed 5 cm from the light source, counts bubbles produced in 1 minute, and repeats at 10 cm and 20 cm; later parts ask how to improve accuracy, explain why constant temperature matters, use the inverse square law I proportional to 1 over d squared to explain light intensity changes, interpret a results table with bubble counts of 129 at 5 cm, 31 at 10 cm, and 8 at 20 cm, and describe how to change the method to investigate carbon dioxide concentration while controlling other variables.
Question text

03 This question is about photosynthesis.

03.1 What is the correct balanced equation for photosynthesis?

[1 mark]

Tick one box.

C6H12O6 + 6O2 6CO2 + 6H2O

O2 + H2O C6H12O6 + CO2

6CO2 + 6H2O C6H12O6 + 6O2

6O2 + 6CO2 6H2O + C6H12O6

03.2 What type of reaction is photosynthesis?

[1 mark]

Tick one box.

Aerobic

Endothermic

Exothermic

Oxidation 9

A student investigated the effect of light intensity on the rate of photosynthesis.

Figure 5 shows the apparatus used.

Figure 5

Sodium hydrogencarbonate solution releases carbon dioxide gas for the pondweed.

This is the method used.

1. Place the pondweed at 5 cm from the light source.

2. Measure the rate of photosynthesis by counting the number of bubbles produced

in 1 minute.

3. Repeat with the pondweed at 10 cm and at 20 cm from the light source.

03.3 Counting the number of bubbles produced in 1 minute is not an accurate way to

measure the rate of photosynthesis.

Suggest two ways the method could be improved to measure the rate of

photosynthesis more accurately.

[2 marks]

03.4 The LED light source does not get hot.

Explain why it is important that the pondweed remains at a constant temperature.

[2 marks]

03.5 Light intensity can be calculated using the inverse square law:

I ∝ 2

d

Where I is light intensity and d is the distance of the pondweed from the light source.

The student placed the pondweed at 5, 10 and 20 cm from the light source.

Explain how light intensity changes as the distance of the pondweed from the light

source is doubled.

You must include calculations in your answer.

[3 marks]

03.6 The student’s results are shown in Table 1

Table 1

Distance of the Number of bubbles

pondweed from the produced in 1 minute

light source in cm

5 129

10 31

20 8

Predict how many bubbles of gas would be produced in 1 minute if the pondweed was

placed 40 cm from the light source.

Give a reason for your prediction.

[2 marks]

03.7 Describe how the student could change the method to investigate the effect of

carbon dioxide concentration on the rate of photosynthesis.

You should include:

• how to change the independent variable

• two control variables.

Use Figure 5 on page 9 to help you answer this question.

[3 marks]

Mark scheme

Show the mark scheme The mark scheme is a table listing answers for questions 03.1 to 03.7 with marks and specification references. It gives the correct photosynthesis equation as 6CO2 + 6H2O yielding C6H12O6 + 6O2, identifies photosynthesis as endothermic, awards marks for improving the practical by measuring volume of gas and increasing the time or repeating and averaging, and explains that temperature affects the rate because photosynthesis is enzyme-controlled. For the inverse square law part it credits showing that doubling distance squares d and quarters light intensity, for the prediction part it accepts 2 bubbles with a reason of very little light energy for photosynthesis, and for the final method question it requires using different concentrations of sodium hydrogencarbonate solution and controlling variables such as distance from light source, temperature, same plant, or equilibration time.

AO /

Question Answers Extra information Mark

Spec. Ref.

03.1 6CO2 + 6H2O C6H12O6 + 6O2 1 AO2

4.4.1.1

03.2 endothermic 1 AO1

4.4.1.1

03.3 measure the volume of gas allow use a measuring cylinder / 1 AO3

released capillary tube / (gas) syringe 4.4.1.2

increase length of time allow sensible length of time 1

allow video the investigation so

you could re-count the bubbles

later

allow repeat the measurement

at each distance several times

and calculate a mean

ignore references to using other

distances

03.4 temperature affects rate of allow correct description of 1 AO3

photosynthesis effect of temperature on rate 4.4.1.2

or

temperature affects rate of

bubble production

(because) reaction / allow high temperatures 1

photosynthesis is controlled by denature enzymes

enzymes

enzymes being denatured must

be linked to high temperature

AO /

Spec. Ref.

03.5 evidence of squaring for two 1 AO2

distances that double: 4.4.1.2

25 and 100

or

100 and 400

calculate 1/d2 for two distances allow 2 marks for these 1 AO2

that double: calculations without working 4.4.1.2

0.04 and 0.01

or ignore calculations for a third

1/25 and 1/100 distance as long as two where

or the distance doubles are correct 12

0.01 and 0.0025

or

1/100 and 1/400

(therefore as distance doubles)

light intensity is quartered 1 AO3

4.4.1.2

03.6 2 (bubbles would be produced) 1 AO3

4.4.1.2

(as) very little light / energy for do not accept no light 1 AO2

photosynthesis to occur 4.4.1.2

allow 2 marks for a quarter of

the bubbles are produced as

light distance doubles so 2

bubbles would be expected

AO /

Spec. Ref.

03.7 (independent variable) 1 AO3

use different concentrations of allow three concentration values 4.4.1.2

sodium hydrogencarbonate ignore different concentrations

solution of carbon dioxide

ignore different amounts of

sodium hydrogen carbonate

solution

(control variables) max 2 marks for control

variables

any two from: 2

• distance from light source allow light intensity

ignore light unqualified

ignore same lamp

• temperature of solution

• same plant allow type / size of plant 13

• time for plant to equilibrate allow time for plant to adjust to

different solution

ignore time unqualified

Total 14

How to answer it

Photosynthesis, Rates and Practical Method

What this question tests

This question checks your knowledge of the balanced photosynthesis equation, the idea that photosynthesis is endothermic, and how to improve a practical investigation so it is more reliable and valid. It also tests interpreting results using the inverse square law, making a prediction from a pattern in data, and describing a method to change one variable while keeping others controlled.

Question 03.1 to 03.7 — Photosynthesis Investigation

Marks available: 14 | The best answers are short, precise, and linked to the mark scheme wording.

03.1 Balanced equation for photosynthesis

Choose the correct symbol equation

✅ Correct answer

6CO₂ + 6H₂O → C₆H₁₂O₆ + 6O₂

This is the balanced equation for photosynthesis.

💡 Key knowledge

  • Photosynthesis uses carbon dioxide and water.
  • It produces glucose and oxygen.
  • It must be balanced, so the number of each type of atom is the same on both sides.

🧠 Exam technique

  • Look for the equation with the correct products and the correct balancing.
  • One mark means you only need the exact correct option.

❌ Common errors

  • Choosing the equation with the wrong arrow direction.
  • Forgetting the coefficient 6 in front of CO₂, H₂O, and O₂.
  • Writing glucose as just CH₂O or similar.

03.2 Type of reaction

Tick the correct box

✅ Correct answer

Endothermic

Photosynthesis absorbs energy from light.

💡 Key knowledge

  • Endothermic = energy taken in from the surroundings.
  • In photosynthesis, light energy is absorbed by chlorophyll.

🧠 Exam technique

Use the meaning of the word: endo = in, so energy goes in.

❌ Common errors

  • Choosing exothermic because “energy is involved”.
  • Choosing aerobic or oxidation because they sound science-like.

03.3 Improving the method

“Counting bubbles in 1 minute is not accurate” — suggest two improvements

✅ Correct answers

  • Measure the volume of gas released instead of counting bubbles.
  • Increase the length of time the bubbles are counted/measured.
  • Repeat the measurement at each distance several times and calculate a mean.
  • Use a measuring cylinder, gas syringe or capillary tube.
  • Video the investigation so bubbles can be recounted later.

💡 Key knowledge

  • Bubble size can vary, so counting bubbles alone is less reliable.
  • Repeated measurements improve reliability.
  • Measuring gas volume is usually more accurate than counting bubbles.

🧠 Exam technique

For 2 marks, give two separate improvements. Make sure each one clearly improves the measurement.

❌ Common errors

  • Saying “use different distances” — this changes the variable, not the accuracy.
  • Saying “count faster” or “watch more carefully” without explaining how that improves accuracy.

03.4 Why keep the pondweed at a constant temperature?

Explain why temperature must be controlled

✅ Correct answer

Temperature affects the rate of photosynthesis / the rate of bubble production.

This is because photosynthesis is controlled by enzymes, and high temperatures can denature them.

💡 Key knowledge

  • Enzymes control reactions in photosynthesis.
  • Temperature changes the enzyme activity and therefore the rate.
  • If temperature changes, it becomes a fair test problem.

🧠 Exam technique

You need a cause and effect answer: temperature changes enzyme action, so the rate changes.

❌ Common errors

  • Just saying “so the plant is healthy” — too vague.
  • Only saying “temperature affects photosynthesis” without explaining why.
  • Mentioning denaturation but not linking it to high temperatures.

03.5 Inverse square law and light intensity

Explain how light intensity changes when the distance is doubled

📐 Calculations: step-by-step

  1. Use the formula pattern: I ∝ 1 / d²
  2. If the distance doubles, square the new distance.
  3. Example distances: 5 cm and 10 cm
  4. Calculate 1 / d²:
    • At 5 cm: 1 / 5² = 1 / 25 = 0.04
    • At 10 cm: 1 / 10² = 1 / 100 = 0.01
  5. Compare the values: 0.01 is one quarter of 0.04
  6. So when distance doubles, light intensity is quartered.

✅ Correct answer

When the distance from the light source is doubled, the light intensity becomes one quarter of its original value.

Using 5 cm and 10 cm: intensity changes from 0.04 to 0.01.

💡 Key knowledge

  • Inverse square law means intensity decreases with the square of distance.
  • If distance doubles, intensity does not halve — it becomes 1/4.
  • Using two doubling distances is enough to show the pattern.

❌ Common errors

  • Saying intensity halves when distance doubles.
  • Forgetting to square the distance.
  • Not showing any working.
  • Mixing up distance and intensity units — light intensity here is a relative value, not in cm.

03.6 Predicting the number of bubbles at 40 cm

Use the results table to make a prediction and explain it

✅ Correct answer

2 bubbles would be produced in 1 minute.

Reason: as the distance doubles, the light intensity is quartered, so the number of bubbles should also fall to about a quarter.

📐 Calculations: step-by-step

  1. From 10 cm to 20 cm, the distance doubles.
  2. 31 bubbles at 10 cm becomes about a quarter at 20 cm: 31 ÷ 4 ≈ 7.75, which matches the result of 8.
  3. From 20 cm to 40 cm, double the distance again.
  4. 8 ÷ 4 = 2

🧠 Exam technique

  • Use the pattern in the table to predict the next value.
  • Give a short reason linked to the inverse square law or the idea of less light for photosynthesis.
  • Round to a whole bubble, since you cannot produce a fraction of a bubble.

❌ Common errors

  • Predicting 0 bubbles — the mark scheme does not accept “no light”.
  • Saying 4 bubbles instead of 2.
  • Giving a prediction with no reason.
  • Ignoring the pattern in the data table.

03.7 Changing the method to investigate carbon dioxide concentration

Describe how to change the independent variable and name two control variables

✅ Correct answer

Independent variable: use different concentrations of sodium hydrogencarbonate solution.

Control variables: any two from:

  • distance from light source
  • temperature of solution
  • same plant
  • time for plant to equilibrate

💡 Key knowledge

  • Sodium hydrogencarbonate releases carbon dioxide.
  • Changing its concentration changes the carbon dioxide available for photosynthesis.
  • A fair test keeps other variables the same.

🧠 Exam technique

  • State exactly how to change the independent variable.
  • Then give two control variables only.
  • Use scientific wording such as “same plant” or “same distance from the lamp”.

❌ Common errors

  • Saying “use more sodium hydrogencarbonate” without mentioning different concentrations.
  • Writing “keep light the same” without stating distance from the light source.
  • Choosing the wrong control variables or repeating the same idea twice.

Examiner insight: what got marks

💡 What distinguished top responses

  • Precise scientific vocabulary: endothermic, enzymes, denature, inverse square.
  • Short, direct explanations linked to the question.
  • Clear calculations with the correct pattern: doubling distance → quarter intensity.
  • For practical questions, students named a better measurement method and a sensible way to improve reliability.

❌ Where students lost marks

  • Confusing endothermic with exothermic.
  • Not giving a reason for why temperature must be controlled.
  • Failing to explain the inverse square relationship.
  • Giving vague practical changes instead of specific methods.

Topics

Biology · Required Practicals · B4: Bioenergetics · Biology Required Practicals

Question and mark scheme from the AQA GCSE Combined Science: Trilogy examination, Biology Paper 1 (Higher), 2018. QuestionVault is an independent revision resource; questions remain the copyright of the awarding body.