AQA GCSE Combined Science: Trilogy Biology Paper 2 (Foundation), 2024: Question 5

11 marks · Standard Demand difficulty · Short Answer

Answer questions about limpets on a sea shore, including constructing a food chain, calculating quadrat area and sample number, evaluating random sampling, planning random quadrat placement, identifying hazards, and suggesting a type of water pollution affecting limpet populations.

Practise this question

Question

The question page is headed Question 05 and introduces limpets as small animals with shells that attach to rocks on sea shores. Figure 9 is a black-and-white photograph of several limpets attached to rocks, with arrows labeling some of them as 'Limpets'. The page then asks seven short questions: give the food chain for algae, crabs and limpets; calculate the area of a square quadrat shown in Figure 10; calculate how many times the quadrat must be used to sample 2% of a 1800 square metre sea shore; explain why throwing a quadrat is not a random sampling method; describe how to plan random placement of the quadrat; suggest one hazard on the sea shore; and suggest one type of water pollution that may affect limpet populations. Figure 10 is a square quadrat drawn as a 5 by 5 grid with a vertical label showing each side is 50 cm.
Question text

05 Limpets are small animals with shells.

Limpets attach to rocks on sea shores.

Figure 9 shows limpets on rocks.

Figure 9

05.1 Limpets eat algae.

Limpets are prey for crabs.

Give the food chain for algae, crabs and limpets.

[1 mark]

Students estimated the population of limpets on a sea shore.

The students were given a square quadrat.

Figure 10 shows the quadrat.

Figure 10

05.2 Calculate the area of the quadrat in m2.

[2 marks]

Area of quadrat =27 m2

05.3 The total area of the sea shore was 1800 m2.

The students sampled 2% of the total area of the sea shore.

Calculate the number of times the students needed to use the quadrat for

the 2% sample.

Use your answer from Question 05.2

[2 marks]

*26* Number of times =

05.4 Explain why throwing a quadrat is not a random method to estimate population size.

Do not refer to safety in your answer.

[2 marks]

05.5 Describe one method the students could use to plan where the quadrat should be

randomly placed each time.

[2 marks]

05.6 Suggest one hazard the students should be aware of when collecting data on

the sea shore.

*27* Do not refer to throwing quadrats in your answer.

[1 mark]

05.7 Populations of limpets are monitored to assess the impact of pollution in water.

Suggest one type of pollution in water that may affect the population of limpets.

[1 mark]

Mark scheme

Show the mark scheme The mark scheme is presented as a series of tables for Question 5 with answer, extra information, mark allocation, and AO/specification reference columns. It gives the food chain 'algae to limpets to crabs', calculates the quadrat area as 2500 square centimetres or 0.25 square metres, and calculates 2% of 1800 square metres as 36 square metres, leading to 144 quadrats using 36 divided by 0.25. Further tables credit explanations that throwing a quadrat causes biased sampling and unrepresentative results, methods using coordinates or a grid with a random number generator for random placement, named sea-shore hazards such as tides, rocks or sewage, and types of pollution such as fertiliser, sewage or toxic chemicals affecting limpet populations.

Question 5

AO /

Question Answers Mark

Spec. Ref.

05.1 alga(e) → limpet(s) → crab(s) 1 AO2

4.7.2.1

direction of arrows must be correct

AO /

Question Answers Extra information Mark

Spec. Ref.

05.2 (50 (cm) × 50 (cm) =) 2500 (cm2) allow conversion cm to m 1 AO2

(50 ÷ 100 =) 0.5 (m) 4.7.2.1

RPA7

2500 allow ¼ (m2) 1

( =) 0.25 (m2)

100 × 100

allow

(0.5 × 0.5 =) 0.25 (m2) for 2

marks

allow (½ × ½ =) ¼ (m2) for 2

marks

AO /

Spec. Ref.

05.3 2

=) 36 (m2) 2

(1800 × allow (1800 × 0.02 =) 36 (m ) 1 AO2

4.7.2.1

RPA7

36 allow (36 × 4 =) 144 (quadrats) 1

( =) 144 (quadrats)

0.25 allow a correct calculation of

number of quadrats with an

incorrect calculation of 2% of

1800

allow ecf from question 05.2

AO /

Spec. Ref.

05.4 (location of sample will be) allow students may aim for 1 AO1

22 biased / chosen limpets 4.7.2.1

allow students may aim to avoid RPA7

limpets

allow students may stand / start

near groups of limpets

or

(location of sample will be)

limited by how far students can

throw

(therefore) results will not be allow population size will be too 1

representative (of (actual) high / low

abundance)

AO /

Spec. Ref.

05.5 tape measure along two 1 AO1

(perpendicular) sides (of sea

4.7.2.1

shore)

or RPA7

allocate co-ordinates (to sea

shore)

or

divide area into a grid

use random number generator allow description of generating 1

random number(s) for example

pulling numbers from container

do not accept throwing quadrats

AO /

Spec. Ref.

allow named example of a

05.6 hazard

any one from: 1 AO3

• water allow sea 4.7.2.1

• tide allow waves RPA7

• rocks allow slippery / uneven surfaces

• quicksand

• named hazardous material on

the sea shore eg needles,

glass

• sewage

• animals allow appropriate named animal 23

for example crab / jellyfish

AO /

Spec. Ref.

05.7 any one from: 1 AO2

• fertiliser 4.7.3.2

• sewage

• toxic chemicals allow named toxic chemical eg

herbicide / pesticide / insecticide

/ DDT / (crude) oil

allow (micro)plastics

allow (dissolved) carbon dioxide

allow heavy metals

allow thermal pollution

Total Question 5 11

How to answer it

Limpets on the sea shore

What this question tests

This question checks your ability to: identify a simple food chain, calculate area, scale up a sample to a whole habitat, explain why a method is not random, describe a random sampling method, and name hazards/pollution. Most marks come from clear GCSE biology facts and correct working.

Question overview

Key skills

  • Food chains: producer → primary consumer → secondary consumer
  • Area calculations using cm and m
  • Percentages and scaling up a sample
  • Sampling methods and fairness/randomness
  • Hazards and water pollution examples

How to score well

  • Use the correct arrow direction in food chains
  • Show units in calculations
  • Use the answer from the previous part if asked
  • Give one clear reason for 1-mark questions

Part (a) / 05.1 — Food chain

Limpets eat algae. Limpets are prey for crabs. Give the food chain for algae, crabs and limpets.

✅ Correct answer

algae → limpets → crabs

1 mark for the correct order and arrow direction.

💡 Key knowledge

  • Algae are the producer.
  • Limpets eat algae, so they are the primary consumer.
  • Crabs eat limpets, so they are the secondary consumer.

🧠 Exam technique

  • Always use arrows to show the direction of energy transfer.
  • The arrow goes from the food to the animal that eats it.

❌ Common errors

  • Reversing the arrows
  • Writing crabs → limpets → algae
  • Leaving out one organism

Part (b) / 05.2 — Area of the quadrat

Calculate the area of the quadrat in m².

📐 Calculations step-by-step

  1. Side length = 50 cm
  2. Area of a square = side × side
  3. 50 × 50 = 2500 cm²
  4. Convert cm² to m²: divide by 10,000
  5. 2500 ÷ 10,000 = 0.25 m²

Answer: 0.25 m²

✅ Correct answer

0.25 m²

2 marks: 1 for calculating area in cm², 1 for converting to m².

🧠 Exam technique

  • Write the units at each stage.
  • You can also use 0.5 m × 0.5 m = 0.25 m² .
  • Either method earns full marks if the working is clear.

❌ Common errors

  • Giving 2500 m² instead of converting units
  • Using 50 × 50 = 100 or another incorrect square area
  • Forgetting that cm² must be converted using squared units

Part (c) / 05.3 — Number of quadrat samples

The total area of the sea shore was 1800 m². The students sampled 2% of the total area. Calculate the number of times the students needed to use the quadrat.

📐 Calculations step-by-step

  1. Find 2% of 1800 m²: 1800 × 2/100 = 36 m²
  2. Each quadrat covers 0.25 m² from part (b)
  3. Number of quadrats = 36 ÷ 0.25 = 144

Answer: 144 quadrats

✅ Correct answer

144 times

2 marks: 1 for finding the sampled area, 1 for dividing by quadrat area.

💡 Key knowledge

  • 2% means 2/100 or 0.02 .
  • Using the answer from part (b) correctly is part of the method.

❌ Common errors

  • Using 1800 × 0.2 instead of 0.02
  • Forgetting to divide by the quadrat area
  • Mixing up area sampled with number of samples

Part (d) / 05.4 — Why throwing a quadrat is not random

Explain why throwing a quadrat is not a random method to estimate population size. Do not refer to safety.

✅ Correct answer

Throwing the quadrat is not random because the students may choose where to throw it, or the throw may be limited by how far they can throw. This means the sample location is biased and not representative.

2 marks: 1 for explaining bias/chosen location, 1 for saying results would not be representative.

💡 Key knowledge

  • A random sample should give every area an equal chance of being chosen.
  • Non-random sampling can overestimate or underestimate the population.

🧠 Exam technique

  • Use science words like biased and representative.
  • Explain the consequence, not just the method.

❌ Common errors

  • Only saying “it is not random” without explaining why
  • Talking about safety, which the question says not to do
  • Describing the throw but not linking it to bias

Part (e) / 05.5 — How to place the quadrat randomly

Describe one method the students could use to plan where the quadrat should be randomly placed each time.

✅ Correct answers

  • Measure two perpendicular sides of the sea shore with a tape measure and use coordinates
  • Divide the area into a grid and use a random number generator
2 marks: 1 for describing the positioning system, 1 for the random method.

💡 Key knowledge

  • Random sampling means each grid square or coordinate has an equal chance.
  • Coordinates or grid references help locate the quadrat fairly.

🧠 Exam technique

  • Give a full method, not just “use randomness”.
  • Examples: random numbers from a generator, numbers drawn from a container.

❌ Common errors

  • “Throw the quadrat” — this does not count here
  • Only saying “use random numbers” without saying what they are used for
  • No clear link to coordinates/grid

Part (f) / 05.6 — One hazard on the sea shore

Suggest one hazard the students should be aware of when collecting data on the sea shore. Do not refer to throwing quadrats in your answer.

✅ Correct answers

  • Water
  • Tide
  • Rocks
  • Quicksand
  • Sewage
  • Animals, e.g. crabs or jellyfish
  • Slippery or uneven surfaces
1 mark for any one valid hazard.

💡 Key knowledge

In GCSE fieldwork, shore habitats can be dangerous because conditions change quickly, surfaces are uneven, and natural hazards may be present.

❌ Common errors

  • Giving an answer too vague to be a hazard
  • Writing “the quadrat” or “throwing” when the question excludes it
  • Not naming a real hazard

Part (g) / 05.7 — Pollution affecting limpets

Populations of limpets are monitored to assess the impact of pollution in water. Suggest one type of pollution in water that may affect the population of limpets.

✅ Correct answers

  • Fertiliser
  • Sewage
  • Toxic chemicals
  • Herbicides / pesticides / insecticides
  • Crude oil
  • Microplastics
  • Heavy metals
  • Thermal pollution
1 mark for one suitable type of water pollution.

💡 Key knowledge

  • Pollution can reduce survival or reproduction of shore animals.
  • Some pollutants poison organisms directly; others change the water conditions.

🧠 Exam technique

  • Name a specific pollutant or pollution type.
  • One clear example is enough for the mark.

❌ Common errors

  • Saying just “pollution” without a type
  • Giving land pollution instead of water pollution
  • Writing vague answers like “dirty water”

Examiner insight: what top answers did

High-scoring features

  • Used the correct biological order in the food chain
  • Showed the area calculation clearly and converted units correctly
  • Used the previous answer from part (b) to scale up the sample
  • Explained why a method was biased and not representative
  • Gave a realistic random sampling method using coordinates or a grid

Where marks were lost

  • Incorrect arrow direction in the food chain
  • Forgetting that 50 cm = 0.5 m
  • Using 2% as 0.2 instead of 0.02
  • Describing a method without saying why it is non-random
  • Giving an example too vague to count as a hazard or pollutant

Quick memory check

Remember

Food chain: algae → limpets → crabs

Quadrat area: 0.25 m²

2% of 1800 m²: 36 m²

Number of quadrats: 144

Best words to use

biased, representative, random, coordinates, grid, pollution

Topics

Biology · B7: Ecology

Question and mark scheme from the AQA GCSE Combined Science: Trilogy examination, Biology Paper 2 (Foundation), 2024. QuestionVault is an independent revision resource; questions remain the copyright of the awarding body.