AQA GCSE Combined Science: Trilogy Biology Paper 2 (Higher), 2024: Question 1

11 marks · Standard Demand difficulty · Short Answer

Answer a set of questions about limpets on a sea shore, including a food chain, quadrat sampling calculations, evaluating random sampling methods, planning random placement of quadrats, identifying hazards, and naming water pollution that could affect limpet populations.

Practise this question

Question

The question page shows a sequence of short biology questions about limpets on a sea shore. Figure 1 is a black-and-white photograph of rocks on a shore with several shell-bearing limpets attached; arrows label some of the limpets. Figure 2 is a square quadrat drawn as a 50 cm by 50 cm grid, followed by questions asking for the food chain algae, limpets and crabs, the area of the quadrat in square metres, the number of quadrat samples needed to cover 2% of a 1800 m² shore, why throwing a quadrat is not random, how to place the quadrat randomly, one hazard on the sea shore, and one type of water pollution that may affect limpet populations.
Question text

01 Limpets are small animals with shells.

Limpets attach to rocks on sea shores.

Figure 1 shows limpets on rocks.

Figure 1

01.1 Limpets eat algae.

Limpets are prey for crabs.

Give the food chain for algae, crabs and limpets.

[1 mark]

Students estimated the population of limpets on a sea shore.

The students were given a square quadrat.

Figure 2 shows the quadrat.

Figure 2

01.2 Calculate the area of the quadrat in m2.

[2 marks]

Area of quadrat =5 m2

01.3 The total area of the sea shore was 1800 m2.

The students sampled 2% of the total area of the sea shore.

Calculate the number of times the students needed to use the quadrat for

the 2% sample.

Use your answer from Question 01.2

[2 marks]

*04* Number of times =

01.4 Explain why throwing a quadrat is not a random method to estimate population size.

Do not refer to safety in your answer.

[2 marks]

01.5 Describe one method the students could use to plan where the quadrat should be

randomly placed each time.

[2 marks]

01.6 Suggest one hazard the students should be aware of when collecting data on

the sea shore.

*05* Do not refer to throwing quadrats in your answer.

[1 mark]

01.7 Populations of limpets are monitored to assess the impact of pollution in water.

Suggest one type of pollution in water that may affect the population of limpets.

[1 mark]

Mark scheme

Show the mark scheme Mark scheme for AQA GCSE Combined Science: Trilogy Biology Paper 2 (Higher), 2024: Question 1

Question 1

AO /

Question Answers Mark

Spec. Ref.

01.1 alga(e) → limpet(s) → crab(s) 1 AO2

4.7.2.1

direction of arrows must be correct

AO /

Question Answers Extra information Mark

Spec. Ref.

01.2 (50 (cm) × 50 (cm) =) 2500 (cm2) allow conversion cm to m 1 AO2

(50 ÷ 100 =) 0.5 (m) 4.7.2.1

RPA7

2500 allow ¼ (m2) 1

( =) 0.25 (m2)

100 × 100

allow

(0.5 × 0.5 =) 0.25 (m2) for 2

marks

allow (½ × ½ =) ¼ (m2) for 2

marks

AO /

Spec. Ref.

01.3 2

=) 36 (m2) 2

(1800 × allow (1800 × 0.02 =) 36 (m ) 1 AO2

4.7.2.1

RPA7

36 allow (36 × 4 =) 144 (quadrats) 1

( =) 144 (quadrats)

0.25 allow a correct calculation of

number of quadrats with an

incorrect calculation of 2% of

1800

allow ecf from question 01.2

AO /

Spec. Ref.

01.4 (location of sample will be) allow students may aim for 1 AO1

biased / chosen limpets 4.7.2.1 7

allow students may aim to avoid RPA7

limpets

allow students may stand / start

near groups of limpets

or

(location of sample will be)

limited by how far students can

throw

(therefore) results will not be allow population size will be too 1

representative (of (actual) high / low

abundance)

AO /

Spec. Ref.

01.5 tape measure along two 1 AO1

(perpendicular) sides (of sea

4.7.2.1

shore)

or RPA7

allocate co-ordinates (to sea

shore)

or

divide area into a grid

use random number generator allow description of generating 1

random number(s) for example

pulling numbers from container

do not accept throwing quadrats

AO /

Spec. Ref.

allow named example of a

01.6 hazard

any one from: 1 AO3

• water allow sea 4.7.2.1

• tide allow waves RPA7

• rocks allow slippery / uneven surfaces

• quicksand

• named hazardous material on

the sea shore eg needles,

glass

• sewage

8 • animals allow appropriate named animal

for example crab / jellyfish

AO /

Spec. Ref.

01.7 any one from: 1 AO2

• fertiliser 4.7.3.2

• sewage

• toxic chemicals allow named toxic chemical eg

herbicide / pesticide / insecticide

/ DDT / (crude) oil

allow (micro)plastics

allow (dissolved) carbon dioxide

allow heavy metals

allow thermal pollution

Total Question 1 11

How to answer it

Limpets, Quadrats and Sampling

What this question tests

Food chains, area calculation, scaling up a sample to a larger area, and understanding why random sampling matters. You also need to describe a valid random method and identify a sea-shore hazard or pollution source.

Question 01.1 — Food chain

✅ Correct answer

algae → limpet → crab

1 mark for the correct organisms in the correct order. The arrows must point the right way to show energy flow.

💡 Key knowledge

  • Food chains show who eats whom.
  • Arrows mean “is eaten by” or show energy transfer.
  • Algae are the producer; limpets eat algae; crabs eat limpets.

🧠 Exam technique

  • Put the living things in a line from food source to predator.
  • Check the direction of each arrow carefully.

❌ Common errors

  • Reversing the arrows.
  • Writing the organisms in the wrong order.
  • Missing out one organism.

Question 01.2 — Area of the quadrat

📐 Calculations: step by step

  1. The quadrat side length is 50 cm.
  2. Area = length × width
    50 cm × 50 cm = 2500 cm²
  3. Convert cm² to m²:
    2500 ÷ 10000 = 0.25 m²

Area of quadrat = 0.25 m²

✅ Correct answer

Both stages are needed for full marks:

  • 2500 cm²
  • 0.25 m²

The mark scheme allows alternative methods, such as converting 50 cm to 0.5 m first, then doing 0.5 × 0.5 = 0.25 m².

💡 Key knowledge

  • 1 m = 100 cm
  • 1 m² = 10,000 cm²
  • Area of a square = side × side

❌ Common errors

  • Using 100 instead of 10,000 when converting cm² to m².
  • Giving the answer in cm² only.
  • Doing 50 ÷ 100 = 0.5 but forgetting to square it.

Question 01.3 — How many quadrats were needed?

📐 Calculations: step by step

  1. Find 2% of the total sea shore area:
    1800 × 2 ÷ 100 = 36 m²
  2. Use the area of one quadrat from 01.2:
    36 ÷ 0.25 = 144
  3. So the number of quadrats is 144.

Number of times = 144

✅ Correct answer

Marks were awarded for:

  • working out the sample area = 36 m²
  • dividing by the area of one quadrat = 0.25 m²

🧠 Exam technique

  • Always use your answer from the previous part if the question says so.
  • Show both the percentage step and the division step.
  • Include units: m² for area and no unit for the number of quadrats.

❌ Common errors

  • Finding 2% incorrectly.
  • Dividing by 1800 instead of by the quadrat area.
  • Forgetting that 2% means 2 ÷ 100.

The mark scheme allows follow-through marks, so a method can still gain credit if part 01.2 was wrong, as long as the rest is consistent.

Question 01.4 — Why throwing a quadrat is not random

✅ Correct answer

  • The sample location may be biased or chosen.
  • Throwing is limited by how far students can throw, so the results may not be representative.

💡 Key knowledge

  • A random sample should give each area an equal chance of being chosen.
  • If students throw, they may hit areas they prefer or avoid areas with limpets.

🧠 Exam technique

  • Use the word biased and explain why that makes the estimate unreliable.
  • For 2 marks, give one point about the method and one point about the result.

❌ Common errors

  • Only saying “it is not random” without explaining why.
  • Talking about safety, which was not allowed here.
  • Not linking the method to the sample being unrepresentative.

Question 01.5 — How to place the quadrat randomly

✅ Correct answer

Any one valid method:

  • Use a tape measure along two perpendicular sides of the sea shore and choose coordinates using random numbers.
  • Allocate co-ordinates to the sea shore and use a random number generator.
  • Divide the area into a grid and choose grid squares at random.

💡 Key knowledge

  • Random sampling means every location should have an equal chance of being chosen.
  • A random number generator can be a computer, calculator, or numbers drawn from a container.

🧠 Exam technique

  • Describe the full method, not just “make it random”.
  • Names of tools matter: tape measure, grid, coordinates, random numbers.

❌ Common errors

  • Saying “throw the quadrat” — this was specifically not accepted.
  • Giving only one vague word like “randomly” with no method.
  • Forgetting to explain how the random location is chosen.

Question 01.6 — One hazard on the sea shore

✅ Correct answer

Any one hazard, for example:

  • water
  • tide
  • rocks
  • quicksand
  • slippery or uneven surfaces
  • hazardous material such as needles or glass
  • sewage
  • animals such as crabs or jellyfish

💡 Key knowledge

  • Sea shores are naturally risky because of moving water, unstable ground, and sharp objects.
  • The mark scheme accepts a named example of a hazard.

🧠 Exam technique

  • Give one clear hazard only.
  • If you name an animal, make it obvious why it is a hazard, e.g. jellyfish.

❌ Common errors

  • Repeating the instruction about throwing quadrats.
  • Giving something too vague, such as “danger”.

Question 01.7 — Type of water pollution

✅ Correct answer

Any one from:

  • fertiliser
  • sewage
  • toxic chemicals

Other accepted examples include pesticide, herbicide, insecticide, DDT, crude oil, microplastics, dissolved carbon dioxide, heavy metals, or thermal pollution.

💡 Key knowledge

  • Pollution can reduce food supply, poison organisms, or damage habitats.
  • Any valid named pollutant that could affect limpets gets the mark.

🧠 Exam technique

  • Use a named pollutant, not a description like “dirty water”.
  • One correct example is enough for 1 mark.

❌ Common errors

  • Giving a type of land pollution instead of water pollution.
  • Writing something too general, such as “waste”.

Big revision takeaway

💡 What top answers did well

  • Gave the exact food chain order.
  • Showed area conversion carefully.
  • Used the 2% sample correctly to scale up.
  • Explained bias clearly for the non-random method.
  • Used precise fieldwork language for random sampling and hazards.

🧠 Final exam tips

  • Check units in every calculation.
  • For ecology questions, think: sample, random, representative, bias.
  • If the question says “use your answer”, always carry it forward.
  • For 1-mark questions, keep the answer short and exact.

Topics

Biology · Required Practicals · B7: Ecology · Biology Required Practicals

Question and mark scheme from the AQA GCSE Combined Science: Trilogy examination, Biology Paper 2 (Higher), 2024. QuestionVault is an independent revision resource; questions remain the copyright of the awarding body.