AQA AS Level Biology Paper 2, June 2025

Every question from AQA AS Level Biology Paper 2, June 2025 (7401): 9 questions, 75 marks, each with its mark scheme and topic.

Original question paper

  1. Question 1 8 marks

    Identify blood vessels in mammalian circulation, explain differences in haemoglobin tertiary structure, and explain mountain goat adaptation using an oxyhaemoglobin dissociation curve.

    Biology · 3.1 Biological molecules · 3.3 Organisms exchange substances with their environment

  2. Question 2 10 marks

    Identify eukaryotic cell organelles and their roles in enzyme secretion, interpret a graph comparing glucose uptake mechanisms with and without oxygen, and calculate an uptake ratio.

    Biology · Practical skills · 3.2 Cells · Data analysis

  3. Question 3 9 marks

    Identify constituent elements of biological molecules, describe the emulsion test for lipids, identify an unsaturated fatty acid, and evaluate the effectiveness of cholestyramine in reducing blood cholesterol concentration.

    Biology · Practical skills · 3.1 Biological molecules · Data analysis · Experimental design

  4. Question 4 7 marks

    Calculate stomatal density from a microscopic field of view, suggest an improvement for reliability, explain a xerophytic adaptation to reduce water loss, and draw conclusions on transpiration rates from a graph.

    Biology · Practical skills · 3.3 Organisms exchange substances with their environment · Experimental design · Data analysis

  5. Question 5 7 marks

    Identify the components of ribosomes, compare the eukaryotic cell cycle with binary fission using provided tabular data, and explain the advantage of checking DNA replication accuracy.

    Biology · 3.1 Biological molecules · 3.2 Cells · 3.4 Genetic information, variation and relationships between organisms

  6. Question 6 10 marks

    Explain enzyme action using the induced-fit model, suggest safety precautions, calculate the surface area comparison of potato discs, and explain the resulting rate of reaction.

    Biology · Practical skills · Required Practicals · 3.1 Biological molecules · Experimental design · Data analysis · AS practicals (1–6)

  7. Question 7 9 marks

    Answer questions on the structure of Vibrio cholerae, protein toxin binding, the mechanism of diarrhoea via osmosis, and calculate cholera mortality from graphical data.

    Biology · Practical skills · 3.1 Biological molecules · 3.2 Cells · Data analysis

  8. Question 8 5 marks

    Explain why infections are more likely after HIV infection based on helper T cell data, and suggest and justify an aseptic technique during bacterial culture.

    Biology · Required Practicals · 3.2 Cells · AS practicals (1–6)

  9. Question 9 10 marks

    Describe how meiosis produces haploid cells and generates genetic variation, and explain how tissue fluid is formed and returned to the circulatory system.

    Biology · 3.3 Organisms exchange substances with their environment · 3.4 Genetic information, variation and relationships between organisms

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