AQA GCSE Biology Biology Paper 2 (Foundation), June 2025

Every question from AQA GCSE Biology Biology Paper 2 (Foundation), June 2025 (8461): 10 questions, 100 marks, each with its mark scheme and topic.

Original question paper

  1. Question 1 9 marks

    Define a dominant allele, determine phenotypes and genotypes in rabbits using a Punnett square, and predict sex chromosome distribution and offspring ratios.

    Biology · B6: Inheritance, Variation and Evolution

  2. Question 2 12 marks

    Describe reflex actions, identify neurones in a reflex arc, and analyze reaction time data from a ruler-drop investigation.

    Biology · Required Practicals · B5: Homeostasis and Response · Required Practicals

  3. Question 3 10 marks

    Identify features of sperm formation, sexual and asexual reproduction in plants, and the advantages of each reproductive method.

    Biology · B1: Cell Biology · B2: Organisation · B6: Inheritance, Variation and Evolution

  4. Question 4 10 marks

    Plot and interpret water balance data, identify kidney processes, and evaluate kidney transplants versus dialysis.

    Biology · B5: Homeostasis and Response

  5. Question 5 8 marks

    Identify DNA bases, calculate the number of amino acids coded by a DNA sequence, identify mutations, and calculate the rate of increase of MRSA cases from a graph.

    Biology · B6: Inheritance, Variation and Evolution

  6. Question 6 8 marks

    Answer questions about a freshwater food web, including identifying energy sources, trophic levels, pyramids of biomass, percentage biomass transfer, and tallying sample data.

    Biology · B7: Ecology

  7. Question 7 11 marks

    Analyze data on biogas production from animal manure and evaluate the effect of adding fish fat.

    Biology · B7: Ecology

  8. Question 8 9 marks

    Explain fossil formation, calculate real organism length from image scale, complete a classification table, and suggest causes of extinction.

    Biology · B6: Inheritance, Variation and Evolution

  9. Question 9 10 marks

    Analyze an investigation into daisy distribution and soil water content along a transect from a tree, calculate a percentage, and evaluate abiotic factors.

    Biology · Required Practicals · B7: Ecology · Required Practicals

  10. Question 10 13 marks

    Plan an investigation into the effect of the direction of light on plant seedling growth and explain how plant tropisms and hormones support plant survival.

    Biology · Required Practicals · B5: Homeostasis and Response · B4: Bioenergetics · Required Practicals

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