AQA GCSE Combined Science: Trilogy Biology Paper 1 (Higher), June 2025: Question 5

10 marks ยท Standard Demand difficulty ยท Extended Answer

Answer questions on plant anaerobic respiration, identify and describe adaptations of palisade mesophyll, and describe the transport of water from the soil to the atmosphere.

Practise this question

Question

Question 5 is divided into four parts. 05.1 asks to identify the two products of anaerobic respiration in plants by ticking two checkboxes from carbon dioxide, ethanol, glucose, lactic acid, and water. Figure 6 displays a cross-sectional diagram of a leaf showing different cell layers with a line pointing to the palisade layer labelled A. 05.2 asks to name tissue A, and 05.3 asks to explain how tissue A is adapted to its function. 05.4 is a 5-mark question asking to describe how water is transported from the soil through plant tissues to the atmosphere.
Question text

05 This question is about plants.

05.1 Some plants can respire anaerobically.

Which are the two products of anaerobic respiration in plants?

[2 marks]

Tick ( ) two boxes.

Carbon dioxide

Ethanol

Glucose

Lactic acid

Water 18

Figure 6 shows the structure of part of a leaf

Figure 6

05.2 What is the name of tissue A?

[1 mark]

05.3 Explain how tissue A is adapted to its function.

[2 marks]

05.4 Plants absorb water from the soil.

Describe how water is transported from the soil through plant tissues to

the atmosphere.

[5 marks]

Extra space

Mark scheme

Show the mark scheme Mark scheme for Question 5 detailing acceptable answers. 05.1 accepts carbon dioxide and ethanol for 1 mark each. 05.2 accepts palisade mesophyll layer for 1 mark. 05.3 gives 1 mark for cells containing many chloroplasts and 1 mark for absorbing more light for photosynthesis. 05.4 awards up to 5 marks for: absorption through root hair cells by osmosis, transport up through xylem to the leaf, movement into spongy mesophyll by osmosis/diffusion, loss through stomata, and evaporation or transpiration to the atmosphere.

Question 5

AO /

Question Answers Extra information Mark

Spec. Ref.

05.1 carbon dioxide 1 AO1

4.4.2.1

ethanol 1

AO /

Spec. Ref.

ignore reference to cells

05.2 palisade (mesophyll layer) 1 AO1

4.2.3.1

AO /

Spec. Ref.

ignore name of tissue

05.3 1 AO1

(cells / tissue) contain many allow (cells) are closely packed 4.2.3.1

chloroplasts together

allow cells / tissue contain a lot

of chlorophyll

(to) absorb more light for 1

photosynthesis

allow for 2 marks (cells / tissue)

contain many chloroplasts to

absorb light for photosynthesis

AO /

Spec. Ref.

05.4 (water absorbed from soil by 1 AO1

osmosis / diffusion) into /

through root hair (cells)

(water) travels up through xylem do not accept water travels up 1 AO1

(vessels) through the phloem

or

(water) through xylem (vessels)

to the leaf

(water) moves into the spongy 1 AO2

mesophyll / layer by diffusion /

osmosis

(water) lost through stomata 1 AO1

(water) lost by evaporation / do not accept translocation 1 AO1 17

transpiration (to atmosphere)

if no other mark awarded allow 1 4.2.3.2

mark for transpiration stream

Total Question 5 10

How to answer it

Plant Respiration, Leaf Structure & Transpiration

๐Ÿ“‹ What This Question Tests

Key GCSE Biology Topics: B4.4.2 (Respiration) and B4.2.3 (Plant Tissues, Organs and Systems)

  • Anaerobic respiration in plants: Recalling the products of fermentation in plant and yeast cells.
  • Leaf organ structure: Identifying the palisade mesophyll layer from a cross-sectional diagram.
  • Tissue adaptations: Explaining how palisade cells are adapted to maximise light absorption for photosynthesis.
  • The Transpiration Stream: Writing a structured, sequential account of how water moves from soil through plant tissues into the atmosphere.
Question 05.1 โ€ข 2 Marks

Products of Anaerobic Respiration in Plants

Tick two boxes to identify the products

โœ… Correct Options

  • Carbon dioxide [1 mark]
  • Ethanol [1 mark]

๐Ÿ’ก Key Knowledge

The word equation for anaerobic respiration in plant cells and yeast is:

glucose โ†’ ethanol + carbon dioxide

In yeast cells, this process is known as fermentation and is economically important in bread-making and alcohol production.

โŒ Common Errors

  • Selecting "Lactic acid": Lactic acid is produced during anaerobic respiration in animal muscles, not in plants or yeast.
  • Selecting "Water": Water is a product of aerobic respiration, not anaerobic respiration.
  • Selecting "Glucose": Glucose is the reactant, not a product.

๐Ÿง  Exam Technique

Notice the prompt explicitly says "Tick (โœ“) two boxes". If you tick more than two, examiners subtract incorrect marks from correct ones, reducing your score.

Question 05.2 โ€ข 1 Mark

Identification of Leaf Tissue A

Identifying the tissue layer located directly beneath the upper epidermis

โœ… Correct Answer

Palisade mesophyll (or palisade layer) [1 mark]

๐Ÿ’ก Key Knowledge: Leaf Cross-Section

From top to bottom, a leaf is organised into:

  1. Waxy cuticle & Upper epidermis: Transparent to let light through.
  2. Palisade mesophyll (Tissue A): Column-shaped cells packed with chloroplasts near the top surface.
  3. Spongy mesophyll: Rounded cells with large air spaces for gas exchange.
  4. Lower epidermis: Contains stomata guarded by guard cells.

โŒ Common Errors

Confusing tissues with cells or organelles. Saying just "chloroplast" or "epidermis" receives 0 marks. The mark scheme notes: ignore reference to cells, but the correct tissue name is required.

๐Ÿง  Exam Technique

Look at the orientation of the cells in the diagram: Tissue A consists of regularly arranged, elongated columnar cells packed closely at the top of the leaf slice.

Question 05.3 โ€ข 2 Marks

Adaptation of Palisade Mesophyll

Explain how tissue A is adapted to its function

โœ… Correct Answer (Mark Scheme Breakdown)

  • Feature: (Contains) many chloroplasts / a lot of chlorophyll / cells are closely packed together [1 mark]
  • Function/Reason: To absorb more light for photosynthesis [1 mark]
Full 2 marks can be awarded in a single coherent sentence: "It contains many chloroplasts to absorb light for photosynthesis."

๐Ÿง  Exam Technique: Feature + Reason

Whenever an exam asks you to explain an adaptation, always structure your answer in two parts:

  1. Structural feature: What does it look like / contain?
  2. Functional benefit: Why does that feature help the plant?

โŒ Common Errors

  • Saying only "it carries out photosynthesis" without stating the structural adaptation (chloroplasts / chlorophyll).
  • Saying "absorbs sunlight" without linking it to the process of photosynthesis.

๐Ÿ’ก Top Tip

Chloroplasts are the organelles; chlorophyll is the green pigment inside them that absorbs light. Both terms are accepted by the mark scheme.

Question 05.4 โ€ข 5 Marks

The Pathway of Water Through a Plant

Describe how water is transported from the soil through plant tissues to the atmosphere

โœ… Full 5-Mark Model Answer (Step-by-Step Pathway)

  1. Step 1 (Uptake): Water is absorbed from the soil into root hair cells by osmosis. [1 mark]
  2. Step 2 (Stem transport): Water travels up the stem through the xylem vessels to the leaves. [1 mark]
  3. Step 3 (Internal leaf movement): Water moves into the spongy mesophyll cells by osmosis / diffusion. [1 mark]
  4. Step 4 (Evaporation): Water evaporates from the surface of mesophyll cells into the air spaces. [1 mark]
  5. Step 5 (Exit to air): Water vapour is lost to the atmosphere through the stomata by transpiration / diffusion. [1 mark]

๐Ÿ’ก The Transpiration Stream

Think of water movement as a continuous unbroken column pulled by evaporation:

Soil โ†’ Root hair โ†’ Xylem โ†’ Mesophyll โ†’ Stomata โ†’ Air

Evaporation from leaves creates tension (transpiration pull), drawing water up through xylem vessels.

โŒ Critical Examiner Traps

  • Xylem vs Phloem: The mark scheme explicitly states: do NOT accept water travels up through the phloem . Phloem transports dissolved sugars (translocation).
  • Transpiration vs Translocation: The mark scheme explicitly states: do NOT accept translocation for water loss.
  • Vague water loss: Stating water is "lost from the plant" is not enough; you must specify stomata and evaporation/transpiration.

๐Ÿง  Exam Strategy for 5-Mark Process Questions

  • Follow chronological order from the start point (soil) to the end point (atmosphere).
  • Always name both the tissue/structure (root hair cell, xylem, stomata) and the transport mechanism (osmosis, evaporation, transpiration).
  • Safety net mark: If a student fails to score other marks, writing "transpiration stream" awards 1 consolatory mark.

๐Ÿ“ Keyword Checklist for Full Marks

  • Root hair cells (absorption from soil)
  • Osmosis (movement across cell membranes)
  • Xylem (transport up stem to leaf)
  • Spongy mesophyll (movement inside leaf)
  • Stomata (exit pores)
  • Evaporation / Transpiration (loss to atmosphere)

Topics

Biology ยท B2: Organisation ยท B4: Bioenergetics

Question and mark scheme from the AQA GCSE Combined Science: Trilogy examination, Biology Paper 1 (Higher), June 2025. QuestionVault is an independent revision resource; questions remain the copyright of the awarding body.